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Cloning and e.pre.sion of NS3 cDNA fragme.t of HCV ge.ome. of He.e. isolate. in ***

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World Journal of Gastroe.te.ology 1998年第2期4卷 73-76页

作者： Zhu, FL Lu, hy Li, Z Qi, ZT Se.ond Mil Me. Univ Dept Microbiol Shanghai 200433 Peoples R China

AIM To obtain gre.te. antige.icity of HCV NS3 prote.n. Me.HODS The.HCV NS3 cDNA fragme.t was amplifie. by re.e.se.transcription polyme.ase.chain re.ction from the.se.a of the.HCV infe.te. patie.ts. The.DNA se.ue.ce.was de.e.mine. by dide.xy me.iate. chain te.mination me.hod using T7 polyme.ase. HCV NS3 prote.n was e.pre.se. in e. coli . Re.ULTS Se.ue.ce.analysis indicate. that the.HCV isolate.of this study be.ongs to HCV Ⅱ; SDS PAGe.de.onstrate. an M r 23800 and an M r 22000 re.ombinant prote.n band which amount to 14% and 11% of the.total bacte.ial prote.ns se.arate.y. We.te.n blotting and e.ISA showe. NS3 prote.n posse.se. gre.te. antige.icity. CONCLUSION Re.ombinant HCV NS3 prote.n was e.pre.se. succe.sfully, which provide. the.basis for de.e.oping HCV diagnostic re.ge.ts.

关键词： he.atitis C virus NS3 ge.e ge.e e.pre.sion DNA, viral viral prote.ns se.ue.ce analysis polyme.ase chain re.ction e.zyme linke. immunosorbe.t assay e.che.ichia coli

Little.girl who conque.e. the."ALPPS''

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World Journal of Gastroe.te.ology 2014年第29期20卷 10208-10211页

作者： Albe.t Chan Patrick hy Chung Ronnie.TP Poon Division of He.atobiliary and Pancre.tic Surge.y and Live. Transplantation Department of SurgeryQueen Mary HospitalThe University of Hong Kong Division of Pe.iatric Surge.y Department of SurgeryQueen Mary Hospitalthe University of Hong Kong

An insufficie.t future.live. re.nant(FLR)is associate. with post-he.ate.tomy live. *** live. partition and portal ve.n ligation for stage.he.ate.tomy(ALPPS)has be.n shown to be.e.fe.tive.for the.induction of rapid FLR hype.trophy so as to improve.the.re.e.tability in patie.ts with insufficie.t *** he.e.y re.ort our e.pe.ie.ce.of this nove. approach for a 6-ye.r-old patie.t with *** tomography showe. a he.atoblastoma me.suring12.5 cm×9.9 cm×11.7 cm in the.right live.(Couinaud se.me.tⅣ,ⅤandⅧ).Volume.ric asse.sme.t of the.FLR i.e.,le.t late.al se.tion was 112.6 mL i.e.,21.2%of the.e.timate. total live. *** vie. of the.small-for-size.FLR,ALPPS was *** ante.ior approach was adopte. for the.in-situ pare.chymal split without mobilisation of the.right *** volume.ry on the.se.e.th postope.ative.day was 160.7 mL,which re.re.e.te. a 46.1%gain in volume.and a FLR/e.LV ratio of 30.2%.A right trise.tione.tomy was pe.forme. on the.e.ghth postope.ative.*** re.ove.y was *** was discharge. on day 16 afte. the.first *** our knowle.ge.this was the.first re.ort that showe. the.applicability of ALPPS to a pae.iatric patie.t.

关键词： Associating live. partition and portal ve.n ligation for stage.he.ate.tomy He.ate.tomy Portal ve.n He.atoblastoma In-situ split

What e.e.Move.e.ts Te.l About Ide.tifying Compound Words in Re.ding and Top-Down e.fe.ts in Re.ding Long Te.ts

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心理与行为研究 2004年第3期2卷 497-504页

作者： Jukka hyn De.artme.t of Psychology University of Turku FIN-20014 Turku Finland

Two line. of re.e.rch on e.e.move.e.ts in re.ding are.summarize.. One.line.of re.e.rch e.amine. how adult re.de.s ide.tify compound words during re.ding. The.othe. line.of re.e.rch de.ls with how a spe.ific re.ding goal influe.ce. the.way long e.pository te.ts are.re.d. Both line. of re.e.rch are.conducte. using Finnish as the.source.language. With re.pe.t to the.first re.e.rch que.tion, it is de.onstrate. that compound words are.re.ognize. e.the. holistically or via the.r compone.ts, de.e.ding on the.le.gth of the.compound word. Re.de.s be.in to proce.s whate.e. information is re.dily available.in the.fove.l vision(i.e., e.the. the.whole.word form or the.initial compone.t). The.se.ond line.of re.e.rch de.onstrate. that(1)a spe.ific re.ding goal is capable.of e.e.ting an e.rly e.fe.t on re.de.s’ e.e.fixation patte.ns,(2)time.course.analyse. base. on e.e.move.e.t patte.ns can re.e.l inte.e.ting individual diffe.e.ce., and(3)working me.ory capacity is linke. to the.e.ficie.cy to strate.ically allocate.atte.tion as we.l as to e.code.information to and re.rie.e.it from the.long-te.m me.ory. It is conclude. that the.e.e.tracking te.hnique.is an e.ce.le.t re.e.rch tool to tap into the.workings of the.human mind during the.compre.e.sion of writte. te.ts.

关键词： e.e move.e.ts word re.ognition compound words te.t compre.e.sion working me.ory capacity.

Disturbance.cue. in fre.hwate. pre. fishe.： Doe. ure. function as an ＇e.rly warning cue. in juve.ile.convict cichlids and rainbow trout？

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Curre.t Zoology 2012年第2期58卷 250-259页

作者： Grant e. BROWN Christophe. D. JACKSON Patrick H. MALKA e.isa JACQUe. Marc-Andre.COUTURIe. De.artme.t of Biology Concordia University 7141 Sherbrooke St. West Montreal Quebec H4B 1R6 CANADA

Fre.hwate. ve.te.rate.and inve.te.rate.pre. spe.ie. commonly re.y on che.ose.sory information, including non-injury re.e.se. disturbance.cue., to asse.s local pre.ation thre.ts. We.conducte. laboratory studie. to （1） de.e.mine.if ure. can function as a disturbance.cue.in juve.ile.convict cichlids and rainbow trout and （2） de.e.mine.if the.background le.e. of ure. influe.ce. the.be.avioral re.ponse.to a subse.ue.t pulse.of ure. （＇background noise. hypothe.is）. In the.first se.ie. of trials, juve. nile.cichlids and trout we.e.e.pose. to ure. at varying conce.trations （0 to 0.5 mg L-1 for cichlids and 0 to 1.0 mg L1 for trout）. Our re.ults sugge.t that both cichilds and trout e.hibite. functionally similar re.ponse. to ure. and conspe.ific disturbance.cue. and that incre.sing the.conce.tration of ure. re.ults in an incre.se.inte.sity of antipre.ator be.aviour. In the.se.ond se.ie. of trials, we.pre.e.pose. cichlids or trout to inte.me.iate.or high conce.trations of ure. （or a distille. wate. control） and the. te.te. for the.re.ponse.to a se.ond pulse.of ure. at at inte.me.iate.or high conce.trations （ve.sus a distille. wate. control）. Our re.ults de.on- strate.that pre.e.posure.to ure. re.uce. or e.iminate. the.re.ponse.to a se.ond pulse.of ure., supporting the.background noise.hy- pothe.is. Toge.he., our re.ults sugge.t that pulse. of ure., re.e.se. by disturbe. or stre.se. individuals, may function as an e.rly warning signal in fre.hwate. pre. spe.ie.

关键词： Disturbance.cue Me.abolic waste. Che.ose.sory risk asse.sme.t Pre.ator-pre. inte.actions Convict cichlids Rainbow trout

Mammalian apoptotic ce.ls e.pre.s spe.ially a nove. Ache.isoform

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Mammalian apoptotic cells express specially a novel AchE-iso...

Pre.pe.ative.Atrial Fibrillation and T-Wave.Inve.sion Associate. with Postope.ative.Pulmonary e.e.a

中国细胞生物学学会第七次会议

作者： MO TW He.hy JP CAe. Shanghai Institute.of Ce.l Biology Chinese Academy of Sciences 200031 Institute.de. Vaisse.ux e. du Sang(IVS) ParisFrance

In this study,ce.l line. HLF,M07e.Me.-01, PC-3,He.A and HOS we.e.e.ploye. to analysis the.Ache.isoform *** re.ult showe. that although the.e.normal ce.ls we.e.nout de.e.te. by the.Ache.staining whe. the. we.e.unde.goi...

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Ope. Journal of Ane.the.iology 2016年第10期6卷 181-185页

作者： Thomas Hong Be.son Ge.rge.Mohamad Hashim De.nis e. Fe.e.man De.artme.t of Ane.the.iology Maimonides Medical Center Brooklyn NY USA

Background: Atrial fibrillation (AF) and T wave.inve.sion (TWI) are.occasionally found on pre.ope.ative.e.e.trocardiograms (e.G) of patie.ts with long standing hy-pe.te.sion (HTN) associate. with le.t ve.tricular hype.trophy (LVH). e.ch of the.e.two pathologie., AF and LVH, can impose.ne.ative.he.odynamic e.fe.ts on the.car-diac function. Case. We.pre.e.t a case.of a patie.t with AF, TWI and long standing HTN that we.t into post-ope.ative.pulmonary e.e.a. Conclusion: e.e. re.urning to base.line.poorly controlle. hype.te.sion, and e.e. more.so during a hype.te.sive.cri-sis, the.r concurre.t pre.e.ce.may inflict e.e. more.de.rime.tal e.fe.t manife.te. by e.e.ation of pulmonary ve.ous pre.sure.and pulmonary e.e.a.

关键词： Pulmonary e.e.a Atrial Fibrillation T Wave.Inve.sion

Mode. for se.wate. fouling and e.fe.ts of te.pe.ature.flow ve.ocity and surface.fre. e.e.gy on se.wate. fouling

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Chine.e.Journal of Che.ical e.gine.ring 2016年第5期24卷 658-664页

作者： Dazhang Yang Jianhua Liu Xiaoxue.e.Linlin Jiang School of e.e.gy and Powe. e.gine.ring University of Shanghai for Science and TechnologyShanghai 200093China Shanghai Ke. Laboratory of Multi-phase.Flow and He.t Transfe. Shanghai 200093China

A kine.ic mode. was propose. to pre.ict the.se.wate. fouling proce.s in the.se.wate. he.t *** ne. mode. adopte. an e.pre.sion combining de.ositional and re.oval be.aviors for se.wate. fouling base. on the.Ke.n–Se.ton *** pre.e.t mode. parame.e.s include.the.inte.rate. kine.ic rate.of de.osition(k d)and the.inte.rate. kine.ic rate.of re.oval(k r),which have.cle.r physical signi ficance.A se.wate.-fouling monitoring de.ice.was e.tablishe. to validate.the.*** e.pe.ime.tal data we.e.we.l fitte. to the.mode.,and the.parame.e.s we.e.obtaine. in diffe.e.t *** and e.X analyse. we.e.pe.forme. afte. the.e.pe.ime.ts,and the.re.ults show that the.main compone.ts of se.wate. fouling are.magne.ium hydroxide.and aluminum *** e.fe.ts of surface.te.pe.ature.flow ve.ocity and surface.fre. e.e.gy we.e.asse.se. by the.mode. and the.e.pe.ime.tal *** re.ults indicate.that the.se.wate. fouling be.ome. aggravate. as the.surface.te.pe.ature.incre.se. in a ce.tain range.and the.se.wate. fouling re.istance.re.uce. as the.flow ve.ocity of se.wate. ***,the.e.fe.t of the.surface.fre. e.e.gy of me.als was analyze.,showing that the.lowe. surface.fre. e.e.gy mitigate. the.se.wate. fouling accumulation.

关键词： Fouling Se.wate. Mode. Surface.te.pe.ature Flow ve.ocity Surface.fre. e.e.gy

On the.Possible.Come.ary Nature.of the.Uchur Cosmic Body(Fall 3.08.1993)

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Advance. in Ge.logical and Ge.te.hnical e.gine.ring Re.e.rch 2023年第3期5卷 16-24页

作者： Tse.movich Vladimir A Ame.in Ivan I Gusiakov Viache.lav K Kirillov Vadim e. Kurazhkovskii Ale.ande. Yu. Ge.physical Obse.vatory“Borok” Branch of the Schmidt Institute of Physics of the EarthRussian Academy of Sciences(GO“Borok”IPE RAS)Yaroslavl regionBorok142152742Russia Institute.of Computational Mathe.atics and Mathe.atical Ge.physics of the.Russian Acade.y of Scie.ce.(IVMiMG SO RAN) 6 Ak.Lavrentieva Ave.Novosibirsk630090Russia Institute.of Te.tonics and Ge.physics name. afte. Yu.Kosygin Russian Academy of Sciencesul.Kim Yu Chen 65Khabarovsk680000Russia

An e.pe.itionary study of the.are. of the.alle.e. impact e.e.t that occurre. on 3.08.1993 in the.are. of the.Lowe. Konkuli Rive.(southe.st of the.Aldan Highlands,Lurikan Range.Russia)was carrie. *** to the.mate.ials of re.ote.se.sing,the.place. of collision with the.e.rth of a cosmic body are.*** the.are. of the.impact of the.shock wave.on the.e.rth’s surface.pe.t sample. we.e.se.e.te.,the.micro probe.analysis of which showe. the.pre.e.ce.of a cosmoge.ic substance.in conce.trations 6-8 time. highe. than the.*** and magne.ite.micro sphe.e.,native.iron,moissanite.and carbon micro tube. coate. with a film consisting of pure.nicke. we.e.*** particular inte.e.t we.e.the.findings of spe.ific Ni film micro structure. that allow us to make.an assumption about the.come.ary nature.of the.Uchur cosmic *** re.e.rche.s associate.the.obse.ve. flights of fire.alls with the.subse.ue.t fall of *** are.trying to find the.massive.body of the.falle. space.***,ofte.,e.e. afte. many ye.rs of se.rching,a massive.cosmic body cannot be.*** happe.e. whe. studying the.site.of the.fall of the.Tunguska cosmic *** this case.it re.ains to be.assume. that the.cosmic body containe. microscopic dust *** structure.and composition of such particle. can only be.studie. using microscopic re.e.rch *** studying the.Uchur cosmic body,the.authors conclude. that it could be.of a come.ary nature.due.to the.findings of spe.ific particle.-thin films of pure.nicke. on the.surface.of plant re.ains of te.re.trial *** hypothe.is arose.from the.re.e.t discove.y of atomic nicke. vapors in come.s.

关键词： Uchur cosmic body Impact e.e.t Cosmoge.ic matte. Microtube. Nicke. films Microstructure. Come.ary nature

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沸石催化甘蔗渣裂解制备多壁碳纳米管

燃料化学学报 2021年第10期49卷 1421-1434页

作者： ABOUL-e.e.N Ate.ya A AWADALLAH Ahme. e.e.-De.OUKI Doaa S ABOUL-GHe.T Noha AK Proce.s De.e.opme.t Division Egyptian Petroleum Research InstituteCairo 11727Egypt e.RI-Nanote.hnology Ce.te. Egyptian Petroleum Research InstituteCairo 11727Egypt

通过催化热解农业废弃物甘蔗渣(SCB),采用两步工艺生产具有显著应用价值的碳纳米管(CNTs)。主要研究沸石催化剂(HZSM-5、HMOR和hy)、热解温度(450−700℃)和SCB/ZSM-5的比例(3−12)对SCB催化热解的影响,确定最佳工艺参数。随后,将第一步... 详细信息

通过催化热解农业废弃物甘蔗渣(SCB),采用两步工艺生产具有显著应用价值的碳纳米管(CNTs)。主要研究沸石催化剂(HZSM-5、HMOR和hy)、热解温度(450−700℃)和SCB/ZSM-5的比例(3−12)对SCB催化热解的影响,确定最佳工艺参数。随后,将第一步产生富含碳的热解产物用于第二步由Co-Mo/MgO催化生长CNTs。通过简单的两步工艺催化热解SCB生产CNTs。SCB的催化热解在半间歇式反应器(外径=4 cm,长度=90 cm)中进行,CNTs的生长在水平反应器(外径=2.5 cm,长度=100 cm)中进行。两个反应器通过玻璃管(直径0.6 cm,长度12 cm)连接并将热解产物从反应器I转移到反应器II。在半间歇式反应器中放入经过预处理的SCB和沸石催化剂,并以20℃/min加热到反应所需温度。随后,将0.5 g Co-Mo/MgO加入到水平反应器中作为CNTs的生长催化剂。在一个典型的实验中,将2 g沸石催化剂、24 g SCB放入半间歇式反应器,研究了不同种类沸石催化剂在500℃温度条件下对SCB热解的影响。接着,使用HZSM-5研究了不同温度(450−700℃)对SCB热解的影响。最后,研究了不同SCB/ZSM-5投料比例(SCB/ZSM-5=3、6、9、12)对SCB热解的影响。此外,水平反应器中的CNTs生长温度恒定为700℃。通过XRD、TPR和TPD进行催化剂表征。XRD谱图证实了催化剂存在MgO和Co_(3)O_(4),以及以小尺寸颗粒形式存在的CoMoO4和MgMoO4。使用XRD数据根据Sche.re.方程计算45%Co-5%Mo/MgO催化剂的平均晶粒尺寸为25.8 nm。TPR分析表明,Co-Mo/MgO催化剂是由未反应的Co_(3)O_(4)以及混合氧化物组成。使用XRD数据,计算得到HZSM-5、HMOR和hy的平均晶粒尺寸分别为43、41和51 nm。NH3-TPD分析表明沸石催化剂存在大量的酸性位点。催化热解SCB结果表明,由hy和HZSM-5催化剂在500℃得到的挥发性产品(生物油和气体)的总收率值分别为34.4%和32.8%,而使用HMOR产生的这些挥发性产品的收率值最低(28.8%)。用HZSM-5催化热解SCB获得了最高的炭产量(23.1%)。然而,使用HMOR和hy热解催化剂产生的炭产量值较低,分别为15.8%和9.8%。使用Te.表征通过两步法生产的碳纳米管,并且测量了CNTs的外径。数据显示,用HZSM-5进行SCB热解产生了直径分布范围较宽的CNTs(10−56 nm),而用HMOR和hy热解催化剂则分别获得了直径较窄的CNTs(13−44 nm,12−28 nm)。拉曼光谱分析表明,使用HZSM-5作为SCB热解的催化剂可以生产最优的CNTs。研究了不同热解温度对CNTs产量的影响。结果显示,将热解温度从450℃提高到500℃,炭产率值从6.7%达到最佳值23.1%,接着增加温度至700℃,炭产量下降至16.7%。随后对不同温度下催化热解形成的炭产品进行Te.表征,结果显示,随着温度的升高炭产品中CNTs的比例下降,CNTs的直径范围变窄。拉曼光谱分析结果表明,在500和700℃形成的CNMs具有最少的缺陷。研究了投料比例对炭产品产量的影响,结果表明,SCB/ZSM-5的比例从3提高到12,生物油和气体的总产量从36.4%下降到32.8%,而生物炭的产量分别从63.6%增加到67.2%。根据催化剂的质量计算得到炭产量的最佳值是使用SCB/ZSM-5比例为6。使用Te.表征不同投料比例生产的炭产品,分析表征结果可得SCB/ZSM-5的比例为6,是形成具有良好质量的致密CNTs的最佳选择。并且不同投料比例的拉曼光谱显示,SCB/ZSM-5比例为6可以生产最优的CNTs。沸石类型(HZSM-5、HMOR和hy)、热解温度(450−700℃)和SCB/ZSM-5比例(3−12)影响CNTs和热解产物(气体、生物油和生物炭)的产量。实验结果表明,获得最高CNTs产量的条件为:热解温度为500℃、SCB/ZSM-5比例为6;获得最高生物油和气体总产量(40%)的条件为:热解温度为700℃、SCB/ZSM-5比例为12。Te.分析表明,使用HZSM-5催化剂在热解温度450℃只生成竹节状碳纳米管(BCNTs),而CNTs和碳纳米洋葱(CNO)是在较高温度热解温度(500−700℃)下产生的。使用SCB/ZSM-5的比例为6时,生成CNTs的直径分布范围最大(7−76 nm)。拉曼光谱分析表明,使用SCB/ZSM-5的比例为6时,形成的CNTs最优。该研究显示,在半间歇式反应器中催化热解SCB是生产CNTs的有效技术,通过对工艺和反应器的优化可以达到更好的效果。

关键词：碳纳米管 Co-Mo/MgO 热解甘蔗渣 ZSM-5沸石