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Cellular preconditioning and mesenchymal stem cell ferroptosis

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World Journal of Stem Cells 2024年第2期16卷 64-69页

作者： Doaa Hussein Zineldeen Mazhar Mushtaq Khawaja husnain Haider Basic Sciences Sulaiman AlRajhi UniversityAlbukairiyah 52736AlQaseemSaudi Arabia Department of Medical Biochemistry and Molecular Biology Faculty of MedicineTanta UniversityTanta 6632110Egypt

In this editorial,we comment on the article published in the recent issue of the World Journal of Stem *** focus on stem cell preconditioning to prevent ferroptosis by modulating the cystathionineγ-lyase/hydrogen sulfide(H_(2)S)pathway as a novel approach to treat vascular disorders,particularly pulmonary *** stem cells are gaining popularity in regenerative medicine due to their unique ability to survive by resisting the harsh,unfavorable microenvironment of the injured *** also secrete various paracrine factors against apoptosis,necrosis,and ferroptosis to enhance cell ***,a regulated form of cell death characterized by iron accumulation and oxidative stress,has been implicated in various pathologies encompassing dege-nerative disorders to *** lipid peroxidation cascade initiates and sustains ferroptosis,generating many reactive oxygen species that attack and damage multiple cellular *** these intertwined mechanisms provi-des significant insights into developing therapeutic modalities for ferroptosis-related *** editorial primarily discusses stem cell preconditioning in modulating ferroptosis,focusing on the cystathionase gamma/H_(2)S ferroptosis *** presents a significant challenge in mesenchymal stem cell(MSC)-based therapies;hence,the emerging role of H_(2)S/cystathionase gamma/H_(2) S signaling in abrogating ferroptosis provides a novel option for therapeutic *** research into understanding the precise mechanisms of H_(2)S-mediated cytoprotection against ferroptosis is warranted to enhance the thera-peutic potential of MSCs in clinical settings,particularly vascular disorders.

关键词： Cell survival Cell therapy Hydrogen sulfide Ferroptosis Preconditioning Stem cells Umbilical cord

High glucose microenvironment and human mesenchymal stem cell behavior

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World Journal of Stem Cells 2024年第3期16卷 237-244页

作者： Muhammad Abdul Mateen Nouralsalhin Alaagib Khawaja husnain Haider Basic Sciences Sulaiman AlRajhi UniversityAlQaseem 52736Saudi Arabia Cellular and Molecular Pharmacology Sulaiman AlRajhi Medical SchoolAl Bukairiyah 51941Saudi Arabia

High glucose(HG)culture conditions in vitro and persistent exposure to hyperglycemia in diabetes patients are detrimental to stem cells,analogous to any other cell type in our *** interferes with diverse signaling pathways,*** target of rapamycin(mTOR)-phosphoinositide 3-kinase(PI3K)-Akt signaling,to impact physiological cellular functions,leading to low cell survival and higher cell apoptosis *** elucidating the underlying mechanism responsible for the apoptosis of adipose tissue-derived mesenchymal stem cells(MSCs),a recent study has shown that HG culture conditions dysregulate mTORPI3K-Akt signaling in addition to mitochondrial malfunctioning due to defective mitochondrial membrane potential(MtMP)that lowers ATP *** organelle-level dysfunction energy-starves the cells and increases oxidative stress and ultrastructural *** of the mitochondrial electron transport chain produces an altered mitochondrial NAD+/NADH redox state as evidenced by a low NAD+/NADH ratio that primarily contributes to the reduced cell survival in *** previous studies have also reported altered mitochondrial membrane polarity(causing hyperpolarization)and reduced mitochondrial cell mass,leading to perturbed mitochondrial *** hostile microenvironment created by HG exposure creates structural and functional changes in the mitochondria,altering their bioenergetics and reducing their capacity to produce *** are significant data,as MSCs are extensively studied for tissue regeneration and restoring their normal functioning in cell-based ***,MSCs from hyperglycemic donors should be cautiously used in clinical settings for cell-based therapy due to concerns of their poor sur-vival rates and increased rates of post engraftment *** hypergly-cemia alters the bioenergetics of donor MSCs,rectifying the loss of MtMP may be an excellent target for future research to restore the normal functioning of MSCs i

关键词： Adipose tissue Apoptosis Bioenergetics Cell survival Cell therapy Hyperglycemia Mitochondria Mesenchymal stem cells Stem cells

Quantitative Behaviour of Guar (Cymopsis tetragnolobus L.) to Various Tillage Systems and Mulches and Soil Physical Properties

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American Journal of Plant Sciences 2016年第7期7卷 1040-1045页

作者： M. ShahidIbni Zamir Muhammad Aamir khan Mazhar Hussain Ihtishamul Haq M. Kamran khan Qamaruz Zaman Usman Afzal Naveed Islam M. Asim Ihtisham Ali husnain khan Khalid Iqbal Department of Agronomy University of Agriculture Faislalabad Pakistan

A field study was conducted to evaluate the effect of organic mulches and tillage practices on growth, yield of cluster bean and soil physical properties. Experiment was comprised of two factors: A (Tillage), B (Mulches). Factor A was assigned to main plot and consisted of two treatments (Minimum tillage and Conventional tillage). Factor B was assigned to sub plot and consisted of four treatments (no mulch, wheat straw mulch, grass clipping mulch and saw dust mulch). The mulching materials were partially incorporated in the field after germination of crop. The experiment was laid out in Randomized Complete Block Design (RCBD) with split plot arrangement having three replications. Control treatment was kept for comparison. All other agronomic practices were kept standardized and consistent for all the treatments. Data regarding growth and yield components were collected and analyzed statistically by fisher analysis of variance and treatment significance was measured by significant difference test at 5% probability level. The factors, tillage and mulches significantly affected growth, yield, yield parameters and soil physical properties. Maximum plant population (31.7 m2), plant height (159 cm), branches per plant (18.9), cluster per plant (15.88), grains per pod (7.3), 1000-grain weight (34.6 g), grain yield (1.9 t•ha-1), biological yield (9.91 t•ha-1) and harvest index (19.15) was recorded in conventional tillage comparative to minimum tillage. Mulches also affected grain yield, and maximum grain yield was recorded in wheat straw mulch (1.88 t•ha-1) followed by grass clipping mulch (1.81 t•ha-1) and saw dust mulch (1.76 t•ha-1) while minimum grain yield was recorded in control without mulch application (1.67 t•ha-1). Tillage and mulches interactively affect pH, soil organic matter contents, electrical conductivity and soil bulk density. Mulches and minimum till

关键词： Guar Mulch Tillage Soil Properties

Mesenchymal stem cells’“garbage bags”at work:Treating radial nerve injury with mesenchymal stem cell-derived exosomes

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World Journal of Stem Cells 2024年第5期16卷 467-478页

作者： Mazhar Mushtaq Doaa Hussein Zineldeen Muhammad Abdul Mateen Khawaja husnain Haider Department of Basic Sciences Sulaiman AlRajhi UniversityAlbukairiyah 52736AlQaseemSaudi Arabia Medical Biochemistry and Molecular Biology Department Faculty of MedicineTanta UniversityTanta 6632110Egypt

Unlike central nervous system injuries,peripheral nerve injuries(PNIs)are often characterized by more or less successful axonal ***,structural and functional recovery is a senile process involving multifaceted cellular and molecular *** contemporary treatment options are limited,with surgical intervention as the gold-standard method;however,each treatment option has its associated limitations,especially when the injury is severe with a large *** advancements in cell-based therapy and cell-free therapy approaches using stem cell-derived soluble and insoluble components of the cell secretome are fast-emerging therapeutic approaches to treating acute and chronic *** recent pilot study is a leap forward in the field,which is expected to pave the way for more enormous,systematic,and well-designed clinical trials to assess the therapeutic efficacy of mesenchymal stem cell-derived exosomes as a bio-drug either alone or as part of a combinatorial approach,in an attempt synergize the best of novel treatment approaches to address the complexity of the neural repair and regeneration.

关键词： Exosome Mesenchymal stem cells Nerve injury Stem cells Secretome Regeneration

Establishing delivery route-dependent safety and efficacy of living biodrug mesenchymal stem cells in heart failure patients

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World Journal of Cardiology 2024年第6期16卷 339-354页

作者： Muhammad Candragupta Jihwaprani Idris Sula Mohamed Ahmed Charbat Khawaja husnain Haider Basic Sciences SRCAl Bukayriyah 52736AlQaseemSaudi Arabia

BACKGROUND Mesenchymal stem cells(MSCs)as living biopharmaceuticals with unique properties,i.e.,stemness,viability,phenotypes,paracrine activity,etc.,need to be administered such that they reach the target site,maintaining these properties unchanged and are retained at the injury site to participate in the repair *** of delivery(RoD)remains one of the critical determinants of safety and *** study elucidates the safety and effectiveness of different RoDs of MSC treatment in heart failure(HF)based on phase II randomized clinical trials(RCTs).We hypothesize that the RoD modulates the safety and efficacy of MSCbased therapy and determines the outcome of the *** To investigate the effect of RoD of MSCs on safety and efficacy in HF *** RCTs were retrieved from six *** endpoints included mortality and serious adverse events(SAEs),while efficacy outcomes encompassed changes in left ventricular ejection fraction(LVEF),6-minute walk distance(6MWD),and pro-B-type natriuretic peptide(pro-BNP).Subgroup analyses on RoD were performed for all study *** Twelve RCTs were ***,MSC therapy demonstrated a significant decrease in mortality[relative risk(RR):0.55,95%confidence interval(95%CI):0.33-0.92,P=0.02]compared to control,while SAE outcomes showed no significant difference(RR:0.84,95%CI:0.66-1.05,P=0.11).RoD subgroup analysis revealed a significant difference in SAE among the transendocardial(TESI)injection subgroup(RR=0.71,95%CI:0.54-0.95,P=0.04).The pooled weighted mean difference(WMD)demonstrated an overall significant improvement of LVEF by 2.44%(WMD:2.44%,95%CI:0.80-4.29,P value≤0.001),with only intracoronary(IC)subgroup showing significant improvement(WMD:7.26%,95%CI:5.61-8.92,P≤0.001).Furthermore,the IC delivery route significantly improved 6MWD by 115 m(WMD=114.99 m,95%CI:91.48-138.50),*** biochemical efficacy outcomes,only the IC subgroup showed a significant reduction in pro-

关键词： Clinical trial Heart failure Mesenchymal stem cells Living biodrug Meta-analysis Stem cells Systematic review

Barley chitinase genes expression revamp resistance against whitefly (Bemisia Tabaci) in transgenic cotton (Gossypium hirsutum L.)

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Journal of Cotton Research 2024年第1期7卷 90-100页

作者： BASHIR Samina YAQOOB Amina BASHIR Rohina BUKHARI Shanila SHAHID Naila AZAM Saira BAKHSH Allah husnain Tayyab SHAHID Ahmad Ali RAO Abdul Qayyum Centre of Excellence in Molecular Biology University of the PunjabLahore 53700Pakistan Department of Botany Kinnaird College for Women UniversityLahore 54000Pakistan Department of Botany Government College UniversityFaisalabad 38000Pakistan Institute of Botany University of the PunjabLahore 54590Pakistan.

Background Chitinase is an enzyme that hydrolyzes chitin,a major component of the exoskeleton of insects,including plant pests like *** present study aimed to investigate the expression of chemically synthesized barley ch1 and chi2 genes in cotton(Gossypium hirsutum)through Agrobacterium-mediated ***-five putative transgenic cotton plants were obtained,out of which fifteen plants successfully survived and were shifted to the *** gene-specific primers,amplification of 447 bp and 401 bp fragments confirmed the presence of the ch1 and chi2 genes in five transgenic cotton plants of the T0 *** five plants were further evalu-ated for their mRNA expression *** T0 transgenic cotton plants with the highest mRNA expression level and better yield performance in field,were selected to raise their subsequent *** The T1 cotton plants showed the highest mRNA expression levels of 3.5-fold in P10(2)for the ch1 gene and 3.7-fold in P2(1)for the chi2 *** in situ hybridization(FISH)confirmed a single copy number of ch1 and chi2(hemizygous)on chromosome ***,the efficacy of transgenes on whitefly was evaluated through an insect bioassay,where after 96 h of infestation,mortality rates of whitefly were calculated to be 78%–80%in transgenic cotton *** number of eggs on transgenic cotton plants were calculated to be 0.1%–0.12 per plant compared with the non-transgenic plants where egg number was calculated to be 0.90–1.00 per *** Based on these findings,it can be concluded that the chemically synthesized barley chitinase genes(ch1 and chi2)have the potential to be effective against insects with chitin exoskeletons,including *** transgenic cotton plants expressing these genes showed increased resistance to whiteflies,resulting in reduced egg numbers and higher mortality rates.

关键词： Chitinase Cotton White fly Transgene Bioassay

Tissue-source effect on mesenchymal stem cells as living biodrugs for heart failure:Systematic review and meta-analysis

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World Journal of Cardiology 2024年第8期16卷 469-483页

作者： Moaz Safwan Mariam Safwan Bourgleh Mohamed Aldoush Khawaja husnain Haider Department of Basic Sciences Sulaiman Al Rajhi UniversityAl Bukairiyah 51941Saudi Arabia

BACKGROUND Mesenchymal stem cells(MSCs),as living biodrugs,have entered advanced phases of clinical assessment for cardiac function restoration in patients with myocardial infarction and heart *** MSCs are available from diverse tissue sources,bone-marrow-derived MSCs(BM-MSCs)remain the most wellstudied cell type,besides umbilical-cord-derived MSCs(UC-MSCs).The latter offers advantages,including noninvasive availability without ethical *** To compare the safety and efficacy of BM-MSCs and UC-MSCs in terms of left ventricular ejection fraction(LVEF),6-min walking distance(6MWD),and major adverse cardiac events(MACEs).METHODS Five databases were systematically searched to identify randomized controlled trials(RCTs).Thirteen RCTs(693 patients)were included using predefined eligibility *** mean differences and odds ratio(OR)for the changes in the estimated treatment *** UC-MSCs significantly improved LVEF vs controls by 5.08%[95%confidence interval(CI):2.20%-7.95%]at 6 mo and 2.78%(95%CI:0.86%-4.70%)at 12 ***,no significant effect was observed for BM-MSCs vs *** significant changes were observed in the 6MWD with either of the two cell ***,no differences were observed for MACEs,except rehospitalization rates,which were lower only with BM-MSCs(odds ratio 0.48,95%CI:0.24-0.97)vs *** UC-MSCs significantly improved LVEF compared with *** advant-Safwan M et ***-source and MSCs as living biodrugs ageous characteristics position them as a promising alternative to MSC-based therapy.

关键词： Cardiovascular disease Heart disease Mesenchymal stem cells Umbilical cord stem cells

Forecast the Influenza Pandemic Using Machine Learning

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Computers, Materials & Continua 2021年第1期66卷 331-340页

作者： Muhammad Adnan khan Wajhe Ul husnain Abidi Mohammed A.Al Ghamdi Sultan H.Almotiri Shazia Saqib Tahir Alyas Khalid Masood khan Nasir Mahmood Department of Computer Science Lahore Garrison UniversityLahore54792Pakistan Systems Limited Lahore54792Pakistan Computer Science Department Umm Al-Qura UniversityMakkah City715Saudi Arabia Department of Computer Science University of Engineering and TechnologyLahore54000Pakistan

Forecasting future outbreaks can help in minimizing their *** is a disease primarily found in animals but transferred to humans through *** 1918,influenza became a pandemic and spread rapidly all over the world becoming the cause behind killing one-third of the human population and killing one-fourth of the pig ***,that influenza became a pandemic several times on a local and global *** 2009,influenza‘A’subtype H1N1 again took many human *** disease spread like in a pandemic *** paper proposes a forecasting modeling system for the influenza pandemic using a feed-forward propagation neural network(MSDII-FFNN).This model helps us predict the outbreak,and determines which type of influenza becomes a pandemic,as well as which geographical area is *** collection for the model is done by using IoT *** model is divided into 2 phases:The training phase and the validation phase,both being connected through the *** the training phase,the model is trained using FFNN and is updated on the *** the validation phase,whenever the input is submitted through the IoT devices,the system model is updated through the cloud and predicts the pandemic *** our dataset,the data is divided into an 85%training ratio and a 15%validation *** applying the proposed model to our dataset,the predicted output precision is 90%.

关键词： Influenza pandemic machine learning prediction influenza influenza pandemic prediction forecast pandemic influenza

Adversarial Training Against Adversarial Attacks for Machine Learning-Based Intrusion Detection Systems

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Computers, Materials & Continua 2022年第11期73卷 3513-3527页

作者： Muhammad Shahzad Haroon husnain Mansoor Ali Department of Computer Science Shaheed Zulfikar Ali Bhutto Institute of Science and TechnologyKarachi75600Pakistan

Intrusion detection system plays an important role in defending networks from security ***-to-end machine learning-based intrusion detection systems are being used to achieve high detection ***,in case of adversarial attacks,that cause misclassification by introducing imperceptible perturbation on input samples,performance of machine learning-based intrusion detection systems is greatly *** such problems have widely been discussed in image processing domain,very few studies have investigated network intrusion detection systems and proposed corresponding *** this paper,we attempt to fill this gap by using adversarial attacks on standard intrusion detection datasets and then using adversarial samples to train various machine learning algorithms(adversarial training)to test their defence *** is achieved by first creating adversarial sample based on Jacobian-based Saliency Map Attack(JSMA)and Fast Gradient Sign Attack(FGSM)using NSLKDD,UNSW-NB15 and CICIDS17 *** study then trains and tests JSMA and FGSM based adversarial examples in seen(where model has been trained on adversarial samples)and unseen(where model is unaware of adversarial packets)*** experiments includes multiple machine learning classifiers to evaluate their performance against adversarial *** performance parameters include Accuracy,F1-Score and Area under the receiver operating characteristic curve(AUC)Score.

关键词： Intrusion detection system adversarial attacks adversarial training adversarial machine learning