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大蒜MtN3/saliva/SWEET基因家族的全基因组鉴定及表达分析

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青海大学学报 2024年第3期42卷 47-55页

作者：许凯路海玲杨雪莲王帅封烁梁健王乐青海大学省部共建三江源生态与高原农牧业国家重点实验室青海西宁810016 青海大学生态环境工程学院青海西宁810016

探究大蒜AsSWEETs基因家族特征和生物学功能,可为大蒜鳞茎发育调控的分子机制提供参考。本研究基于大蒜“二水早”全基因组数据,通过HMM搜索对AsSWEETs成员进行筛选和鉴定,并利用实时荧光定量PCR分析其在不同激素处理下的表达模式。结... 详细信息

探究大蒜AsSWEETs基因家族特征和生物学功能,可为大蒜鳞茎发育调控的分子机制提供参考。本研究基于大蒜“二水早”全基因组数据,通过HMM搜索对AsSWEETs成员进行筛选和鉴定,并利用实时荧光定量PCR分析其在不同激素处理下的表达模式。结果表明:在大蒜基因组中共鉴定到35个AsSWEETs基因,均为疏水蛋白,含2~7个不等的跨膜螺旋,除AsSWEET 1、AsSWEET 2位于scaffold外,其余均分布于染色体上,成员间差异显著。综合系统进化和表达模式发现,AsSWEET5、14、21、23、26、28(第III支系,转运蔗糖)可能是参与鳞茎膨大的主要调控基因;AsSWEET 2、30、33(IV分支)的表达量在鳞茎发育不同阶段变化较大,可能参与鳞茎发育过程中的果糖代谢调节。qRT-PCR定量表明,在不同激素处理下,AsSWEET5、11、16呈现出不同的表达模式,预示AsSWEETs家族成员功能存在分化和差异。综上,大蒜AsSWEET s基因的糖转运活性参与了鳞茎的生长发育,有助于解释鳞茎膨大发育过程。

关键词：大蒜(Allium sativum L.) AsSWEETs基因家族生物信息学分析表达分析

Cr3和Cr5系支承辊大型锻件脱氧工艺研究

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江西冶金 2024年第1期44卷 25-33页

作者：何川张延玲王冠博戴乐张威风石如星北京科技大学钢铁冶金新技术国家重点实验室北京100083 洛阳中重铸锻有限责任公司洛阳471039

由于Cr3和Cr5系支承辊钢液中铬含量增加会抑制铝脱氧反应的进行。本文以Cr3和Cr5系支承辊冶金过程中脱氧工艺为研究背景,通过实验室脱氧实验,利用FactSage 8.1软件计算Cr3和Cr5系轧辊钢中氧含量,绘制铝脱氧平衡曲线,将Wagner模型与炉渣... 详细信息

由于Cr3和Cr5系支承辊钢液中铬含量增加会抑制铝脱氧反应的进行。本文以Cr3和Cr5系支承辊冶金过程中脱氧工艺为研究背景,通过实验室脱氧实验,利用FactSage 8.1软件计算Cr3和Cr5系轧辊钢中氧含量,绘制铝脱氧平衡曲线,将Wagner模型与炉渣共存理论相结合,建立了钢渣反应模型,探讨铬含量对钢液中铝脱氧效果的影响。结果表明,钢液中铬含量在一定范围内增加会抑制铝脱氧反应进行;在精炼过程中,当Cr3系支承辊中铝含量控制在0.03%左右、Cr5系支承辊中铝含量控制在0.05%左右时,可将氧含量有效控制在10^(-5)以下;而与之相匹配的最佳炉渣成分的碱度为2.5~3.0,w(Al_(2)O_(3))>20%。

关键词：轧辊钢脱氧工艺铬含量钢渣反应模型炉渣共存理论

First-principle study of Ba isotopic fractionation during ion exchange processes

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Acta Geochimica 2022年第1期41卷 121-131页

作者： Xin-Yue Ji Yan-Fang Wang le-Cai Xing Jian Liu Peng-Dong Wang Tian-Di Zhang Hao-Nan Zhao School of Earth Science and Engineering Hebei University of EngineeringHandan 056038China Key Laboratory of Resource Survey and Research of Hebei Province Hebei University of EngineeringHandan 056038China

The potential utilization and development of the Ba isotope tool depend on an accurateδ^(137/134)Ba determination of the *** the chemical purification,whether the adsorption process on the surface of the ionexchange resin could lead to the Ba isotopic fractionation and the degree of fractionation directly influence the accurateδ^(137/134)Ba *** the present work,first-principles calculations based on the density functional theory were used to quantify the Ba isotopic equilibrium fractionation factor between the aqueous solution and the resin in the acid leaching *** constructing and optimizing the geometric configurations of Ba-containing species,Ba(H_(2)O)_(n)^(2+),Ba(H_(2)O)_(n)Cl_(2),Ba(H_(2)O)_(n)(NO_(3))2,and the adsorbed Ba^(2+)on the surface of the resin,extracting the harmonic vibrational frequencies,we finally at 298 K obtained the fractionations,Δ^(137/134)Ba_(soln-ads)=0.07‰,Δ^(137/134)Ba_(Ba(H_(2)O)_(n)Cl_(2)-ads)=0.05‰,andΔ^(137/134)-Ba^(Ba(H_(2)O)_(n)(NO_(3))2-ads)=0.02‰.Overall,there were almost no Ba isotope fractionations during *** the Ba isotope fractionation can be magnified by the Rayleigh fractionation process in purification,the difference inδ137/134Ba between the initial and final stages did not exceed0.060‰(or 0.045‰)when leaching the standard sample with HCl or HNO_(3),which is equal to or less than the accuracy of Ba isotopic *** a common yield of89.75%,Ba isotopic fractionation induced by incomplete recovery was 0.015‰for HCl(or 0.011‰for HNO_(3)).Finally,if the influence of an incomplete recovery on theδ137/134Ba determination needs to be ignored,the recovery is suggested to be not less than 67%for HCl(or 46%for HNO_(3)).

关键词： Ion exchange Barium Equilibrium isotopic fractionation First-principles calculations Rayleigh fractionation

Research on Cheating Detection Method of Programming Source Codes by Self-Organizing Maps

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Increasing angiotensin-converting enzyme(ACE)2/ACE axes ratio alleviates early pulmonary vascular remodeling in a porcine model of acute pulmonary embolism with cardiac arrest

中国电子商情·通信市场 2013年第1期 53-58页

作者： Guojin Zhu le Liu

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World Journal of Emergency Medicine 2022年第3期13卷 208-214页

作者： Hong-li Xiao Lian-xing Zhao Jun Yang Nan Tong le An Guo-xing Wang Miao-rong Xie Chun-sheng Li Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of Emergency Internal Medicine the Affiliated Hospital of Qingdao UniversityQingdao 266000China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100050China

BACKGROUND:Acute pulmonary embolism(APE)with cardiac arrest(CA)is characterized by high mortality in emergency due to pulmonary arterial hypertension(PAH).this study aims to determine whether early pulmonary artery remodeling occurs in PAH caused by massive APE with CA and the protective effects of increasing angiotensin-converting enzyme(ACE)2-angiotensin(Ang)(1-7)-Mas receptor axis and ACE-Ang II-Ang II type 1 receptor(AT1)axis(ACE2/ACE axes)ratio on pulmonary artery lesion after return of spontaneous circulation(ROSC).METHODS:To establish a porcine massive APE with CA model,autologous thrombus was injected into the external jugular vein until mean arterial pressure dropped below 30 mmHg(1 mmHg=0.133 kPa).Cardiopulmonary resuscitation and thrombolysis were delivered to regain spontaneous *** were divided into four groups of five pigs each:control group,APE-CA group,ROSC-saline group,and ROSC-captopril group,to examine the endothelial pathological changes and expression of ACE2/ACE axes in pulmonary artery with or without ***:Histological analysis of samples from the APE-CA and ROSC-saline groups showed that pulmonary arterioles were almost completely occluded by accumulated endothelial *** blotting analysis revealed a decrease in the pulmonary arterial ACE2/ACE axes ratio and increases in angiopoietin-2/angiopoietin-1 ratio and expression of vascular endothelial growth factor(VEGF)in the APE-CA group compared with the control *** significantly suppressed the activation of angiopoietin-2/angiopoietin-1 and VEGF in plexiform lesions formed by proliferative endothelial cells after *** also alleviated endothelial cell apoptosis by increasing the B-cell lymphoma-2(Bcl-2)/Bcl-2-associated X(Bax)ratio and decreasing cleaved caspase-3 ***:Increasing the ACE2/ACE axes ratio may ameliorate pulmonary arterial remodeling by inhibiting the apoptosis and proliferation of endothelial cells after ROSC i

关键词： Acute pulmonary embolism Cardiac arrest Early pulmonary vascular remodeling Angiotensin-converting enzyme

基于多元感知信息融合的电抗器故障声纹诊断研究

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环境技术 2024年第2期42卷 121-126页

作者：李子彬王理丽王子乐包正红陈尧国网青海省电力公司电力科学研究院西宁810008

为提高电抗器故障诊断准确率,提出一种基于多元感知信息融合的诊断方法。方法通过采用PSO(Particle SwarmOptimization)算法优化LSTM(LongShort-TermMemory)网络的神经元个数与dropout值改进LSTM网络,并利用改进LSTM网络对电抗器故障声... 详细信息

为提高电抗器故障诊断准确率,提出一种基于多元感知信息融合的诊断方法。方法通过采用PSO(Particle SwarmOptimization)算法优化LSTM(LongShort-TermMemory)网络的神经元个数与dropout值改进LSTM网络,并利用改进LSTM网络对电抗器故障声纹进行分类识别,实现了电抗器故障诊断。仿真结果表明,采用PSO算法改进LSTM网络,可提高LSTM网络的收敛速度和分类识别精度;采用PSO算法改进LSTM网络模型对电抗器故障声纹进行分类识别,可有效诊断额定预紧力松动、单边夹件松动、双边夹件松动等不同类型的电抗器故障,且具有较高的准确率,平均准确率约为98%;相较于SVM、CNN、BP模型以及标准LSTM网络模型,所提PSO算法改进的LSTM网络模型对电抗器故障声纹诊断的准确率更高,具有明显优势。

关键词：电抗器故障声纹诊断 LSTM网络 PSO算法

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Un oiseau alle mand

法语国家与地区研究 2003年第1期 3-8页

作者： Michèle Kahn

Deuteration triggered downward shift of dielectric phase transition temperature in a hydrogen-bonded molecular crystal

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Chinese Chemical letters 2022年第3期33卷 1422-1424页

作者： Bei-Dou Liang Tong Jin le-Ping Miao Chao-Yang Chai Chang-Chun Fan Xiang-Bin Han Wen Zhang Jiangsu Key Laboratory for Science and Applications of Molecular Ferroelectrics and School of Chemistry and Chemical Engineering Southeast UniversityNanjing 211189China

Deuteration of hydrogen-bonded phase transition crystals can increase the transition temperatures due to the isotope effect. But rare examples show the opposite trend that originates from the structural changes of the hydrogen bond, known as the geometric H/D isotope effect. Herein, we report an organic crystal, diethylammonium hydrogen 1,4-terephthalate, exhibits a reversible structural phase transition and dielectric switching. Structural study shows the cations reside in channels formed by one-dimensional hydrogen-bonded anionic chains and undergo an order-disorder transition at around 206 K. The deuterated counterpart shows an elongation of the O…O hydrogen bond by about 0.005 A. this geometric isotope effect releases the internal pressure of the anionic host on the cation guests and results in a downward shift of the phase transition temperature by 10 K.

关键词： Dielectric phase transition Stimulus responsive material Deuteration Geometric isotope effect Downward shift of transition temperature

靶向人CD70的嵌合抗原受体巨噬细胞制备及其对肾癌的杀伤作用的研究

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肿瘤预防与治疗 2024年第3期37卷 195-203页

作者：张雅婷杜玉菲汪乐肖漪周一鸣中山大学孙逸仙纪念医院基础与转化医学研究中心广州510120

目的:本研究旨在探讨稳定感染靶向人CD70蛋白的嵌合抗原受体巨噬细胞(chimeric antigen receptor ma-cophages,CAR-M)对人肾癌细胞系(786-O、OS-RC-2和ACHN)的体外杀伤作用,开发靶向人CD70的肾癌细胞的新型细胞疗法。方法:将含人CD70抗... 详细信息

目的:本研究旨在探讨稳定感染靶向人CD70蛋白的嵌合抗原受体巨噬细胞(chimeric antigen receptor ma-cophages,CAR-M)对人肾癌细胞系(786-O、OS-RC-2和ACHN)的体外杀伤作用,开发靶向人CD70的肾癌细胞的新型细胞疗法。方法:将含人CD70抗体scFv片段的CAR序列插入含有GFP序列的慢病毒质粒中,制备CD70-scFV-CAR-GFP的慢病毒。通过慢病毒感染小鼠巨噬细胞RAW264.7制备CD70-CAR-M,通过流式细胞仪分选出含有绿色荧光标记的CD70-CAR-M细胞并在体外筛选稳定表达细胞株。将CD70-CAR-M细胞与不同类型的肾癌类器官按照1∶1的比例分别共培养后,利用流式细胞术检测CD70-CAR-M对肾癌细胞的杀伤作用。qRT-PCR检测CD70-CAR-M在和肾癌细胞系共培养后TNF-α和TGF-β的表达水平改变情况。结果:通过测序确认了CD70-scFV序列,并成功制备了相应的慢病毒。利用该慢病毒感染巨噬细胞RAW264.7,通过qRT-PCR技术证明CD70-CAR分子稳定表达在RAW264.7。通过和不同类型的肾癌细胞系共培养后,流式细胞术结果显示,与对照组相比,CD70-CAR-M共培养后的肿瘤细胞数量明显减少。同时发现CD70-CAR-M的TNF-α表达水平明显增加,而TGF-β的表达水平明显降低,从而对肿瘤产生较强的杀伤作用。结论:成功构建了靶向人CD70的CAR-M巨噬细胞,并且在体外证明其能对CD70阳性肾癌细胞产生杀伤作用。

关键词：肾癌 CAR-M细胞 CD70