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Tobacco plant: A possible key to Ebola vaccine

Journal of Coastal Life Medicine 2017年第5期5卷 206-211页

作者： Kulsoom Zahara Yamin Bibi Maryam Ajmal huma mehreen sadaf Fatima Bibi Nadia Sardar sadaf Laraib Iqra Riaz Department of Botany PirMehr Ali Shah Arid Agriculture UniversityRawalpindiPakistan

In 2014, the World Health Organization was notified of an outbreak of a communicable disease characterized by fever, severe diarrhea, vomiting, and a high fatality rate in Guinea. Virologic investigation identified Zaire ebolavirus (EBOV) as the causative agent. Approximately 30 years after the first epidemic, there is no vaccine or therapeutic medication approved to counter the Ebola virus. In this review, it emphasized that plant based antibodies can be an effective cure to Ebola hemorrhagic fever. Genetically modified Nicotiana benthamiana is used to manufacture ZMapp drug by Kentucky BioProcessing. ZMapp three-antibody cocktail binds to the Ebola virus to deactivate it and provides simulated immune response against sugar-tagged proteins on the outside of the Ebola virus, which had verified efficacy against Ebola infection in nonhuman primates and also successfully treated two American medical workers infected with Ebola virus disease.

关键词： Zaire ebolavirus Nicotiana benthamiana ZMapp

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Plantlets Regeneration via Somatic Embryogenesis from the Nucellus Tissues of Kinnow Mandarin (Citrus reticulata L.)

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American Journal of Plant Sciences 2016年第6期7卷 798-805页

作者： Mubashir Hussain Naveed Iqbal Raja Muhammad Iqbal Anam Iftikhar huma mehreen sadaf Sidra Sabir Muhammad Asim Sultan Muhammad Nasim Ashraf Faz Department of Botany PMAS Arid Agriculture University Rawalpindi Pakistan Department of Botany University of Sargodha Sargodha Pakistan Department of Botany Government College University Faisalabad Pakistan

Studies were initiated to explore the role of nucellus tissues and growth regulators in plantlets regeneration via somatic embryogenesis of Kinnow mandarin [Citrus reticulata L. (Blanco)]. Nucellus tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins, cytokinins and malt extract for primary callus induction. The best response for primary callus induction (90%) was obtained when MS medium was supplemented with 5 mg/l 2,4-D and 500 mg/l malt extract. Best results for embryogenic callus induction (80%) were obtained in C8 medium. The induction of somatic embryos was highest when MS medium was supplemented with 1 mg/l BAP and maturation of somatic embryos occurred when MS medium was supplemented with 5 mg/l 2,4-D and 1 mg/l BAP. Maximum plantlets were regenerated (92%) from the somatic embryos on half strength MS medium with no hormones. The plantlets were successfully acclimatized in different potting mixtures and highest survival rate (100%) was achieved in potting mixture containing sand and peat moss (2:1).

关键词： Tissue Culture MS Media Citrus Nucellus Tissue Callogenesis Somatic Embryos

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A probe into the medicinal potential of Viola canescens-A threatened medicinal plant from Himalaya

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Journal of Coastal Life Medicine 2016年第7期4卷 575-579页

作者： Sidra Sabir Abida Akram Naveed Iqbal Raja Zia-ur-Rehman Mashwani Sohail huma mehreen sadaf Mubashir Hussain Iqra Riaz Nabeela Ahmad Ejaz Ahmed Department of Botany PMAS-Arid Agriculture UniversityRawalpindiPakistan

Viola canescens *** *** a perennial herb belonging to family Violaceae,and it is almost cosmopolitan in *** plant is widely used in Ayurveda and Unani medicinal systems for curing various ailments,most commonly for cough and *** studies releaved that this plant is rich in secondary *** plant revealed significant antimicrobial,anti-inflamatory,antioxidant,hepatoprotective,laxative,analgesic as well as antitumor *** to all these important pharmacological activities,market demand of Viola canescens is increasing day by day and this plant is facing tremendous over exploitation and becomes a threatened plant according to International Union for Conservation of Nature and Natural *** present review compiles that the ethnobotanical,phytochemical and pharmacological aspects of the plant need to be conserved.

关键词： Viola canescens Medicinal systems Secondary metabolites Pharmacological activities

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