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The selection and stability analysis of stable and high Taxol-producing cell lines from Taxus cuspidata

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Journal of Forestry Research 2018年第1期29卷 65-71页

作者： Shujie wang hujun wang Tong Li Chun Li Yajun Zhou Xiangmei Zhong College of Biological and Agricultural Engineering Jilin University Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University College of Food Science and Engineering Jilin University

In this study to screen for stable, high Taxolproducing cell lines（CL5, CL12, and CL21） of Taxus cuspidata, stem tissues were used to induce calli, which were then subcultured nine times to establish suspension cell cultures. From 97 cell lines obtained from conditioned cultures, 10 cell lines with high Taxol content were selected. Stability analyses on solid and liquid B5 media were then used to obtain lines that stably produced high levels of Taxol. Fresh biomass and Taxol production of the ninth generation became stable. Taxol content of selected CL5, CL12, and CL21 samples was 0.0448, 0.0477, and0.0428% of dry mass（DW）, respectively. Proliferation of CL5, CL12 and CL21 was 346.3, 382.5, and 409.2%,respectively. From work over about 2 years, the three cell lines appear suitable for mass production of Taxol,promoting the industrialisation and commercial-scale production of Taxol using cell culture.

关键词： Paclitaxel Taxus cuspidata Taxol Cell lines Stability analysis

Bioinspired directional structures for inhibiting wetting on super-melt-philic surfaces above 1200°C

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International Journal of Extreme Manufacturing 2024年第4期6卷 337-346页

作者： hujun wang Xiuyuan Zhao Zhengcan Xie Biao Yang Jing Zheng Kai Yin Zhongrong Zhou Tribology Research Institute State Key Laboratory of Rail Transit Vehicle SystemSchool of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031People's Republic of China Hunan Key Laboratory of Nanophotonics and Devices School of PhysicsCentral South UniversityChangsha 410083People's Republic of China

Over the past two decades,superhydrophobic surfaces that are easily created have aroused considerable attention for their superior performances in various applications at room ***,there is a growing demand in special fields for the development of surfaces that can resist wetting by high-temperature molten droplets(>1200°C)using facile design and fabrication ***,bioinspired directional structures(BDSs)were prepared on Y2O3-stabilized ZrO2(YSZ)surfaces using femtosecond laser *** from the anisotropic energy barriers,the BDSs featured with no additional modifiers showed a remarkable increase from 9.2°to 60°in the contact angle of CaO–MgO–Al2O3–SiO2(CMAS)melt and a 70.1%reduction in the spreading area of CMAS at 1250°C,compared with polished super-CMAS-melt-philic YSZ ***,the BDSs demonstrated exceptional wetting inhibition even at 1400°C,with an increase from 3.3°to 31.3°in contact angle and a 67.9%decrease in spreading *** work provides valuable insight and a facile preparation strategy for effectively inhibiting the wetting of molten droplets on super-melt-philic surfaces at extremely high temperatures.

关键词： directional structures bioinspired design inhibiting wetting super-melt-philic superhydrophobic

Enamel matrix proteins in promoting saliva lubrication

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Friction 2024年第10期12卷 2298-2312页

作者： hujun wang Yue TANG Haonan QIU Jingyang HU Yuan SU Jing ZHENG Zhongrong ZHOU Tribology Research Institute School of Mechanical Engineering and Key Laboratory of Advanced Technologies of Materials(Ministry of Education)Southwest Jiaotong UniversityChengdu 610031China

Anti-wear performance of human enamel in the mouth is closely related to the lubrication of salivary *** is well known that the inorganic hydroxyapatite(HA)of the enamel plays an important role in the adsorption and pellicle-forming of salivary proteins on the enamel,but the role of enamel matrix proteins remains *** this study,the adsorption and lubrication behavior of salivary proteins on original,heated,and deproteinated enamel surfaces was comparatively investigated using an atomic force microscopy and nano-indentation/scratch *** with that on the original enamel surface,the adsorption and lubrication behavior of salivary proteins remains almost unchanged on the heated enamel surface(where the enamel matrix proteins are denatured but the size of HA crystalline nanoparticles keeps constant)but exhibits an obvious compromise on the deproteinated enamel surface(where the enamel matrix proteins are removed and agglomeration of HA crystallites occurs).The HA agglomeration weakens the electrostatic interaction of enamel surfaces with salivary proteins to cause a distinct negative influence on the adsorption and pellicle-forming of salivary ***,the negative effect is confirmed with a quartz crystal microbalance with *** summary,by regulating enamel nanostructure for appropriate electrostatic interactions between salivary proteins and enamel surfaces,the enamel matrix proteins play an essential role in the adsorption and pellicle-forming of salivary proteins on human enamel,and then contribute to saliva lubrication,which provides the enamel with an anti-wear *** findings will promote and assist the design of enamel-inspired anti-wear materials.

关键词： enamel matrix proteins hydroxyapatite(HA)crystalline nanoparticles salivary proteins adsorption lubrication

Effect of elicitors, precursors and metabolic inhibitors on paclitaxel production by Taxus cuspidata cell culture

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Journal of Forestry Research 2016年第6期27卷 1257-1263页

作者： Shujie wang Chun Li hujun wang Xiangmei Zhong Jing Zhao Yueying Tong Yajun Zhou College of Biological and Agricultural Engineering Jilin University Key Laboratory of Bionic Engineering (Ministry of Education) Jilin University College of Food Science and Engineering Jilin University

In this study, using Taxus cuspidata as a raw material, we obtained stable high-yielding cell lines by subculturing and quantified paclitaxel content using ultra- sonic extraction combined with TLC-UV spectrophotom- etry. In single factor and multiple factors tests to optimize design and study the effects of elicitors, precursors, and metabolic inhibitors on paclitaxel production by Taxus cuspidata cells, paclitaxel production reached 4.32 mg/L when 100 μmol/L methyl jasmonate, 20 mg/L salicylic acid, 400 mg/L phenylalanine and 2 mg/L gibberellin （GA3） were added to the culture medium of suspension cells. When adding metabolic adjustment factors on the 7th day of culture, extra- and intracellular paclitaxel production was the highest at 4.855 mg/L, paclitaxel release rate was 10.48 %, fresh mass and paclitaxel production of cell increased, respectively, by 6.08 and 11.57 %. By controlling the anabolism of paclitaxel, paclitaxel yield was significantly improved.

关键词： Elicitor Precursor Metabolic inhibitor Paclitaxel Cell culture Taxus cuspidata

Experimental investigation and feasibility analysis of a thermophotovoltaic cogeneration system in high-temperature production processes

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Frontiers in Energy 2013年第2期7卷 146-154页

作者： Jianxiang wang Hong YE Xi WU hujun wang Xiaojie XU Department of Thermal Science and Energy Engineering University ofScience and Technology of China Hefei 230027 China Department of Thermal Science and Energy Engineering University ofScience and Technology of China Hefei 230027 China School ofPhysics Science and Technology Soochow University Suzhou 215006China School of Physics Science and Technology Soochow UniversitySuzhou 215006 China

The experimental I- Vcharactefistics ofa Si cell module in a thermophotovoltaic （TPV） system were investigated using SiC or Yb203 radiator. The results demonstrate that the short-circuit current increases while the open-circuit voltage, along with the fill factor, decreases with the cell temperature when the radiator temperature increases from 1273 to 1573 K, leading to a suppressed increase of the output power of the system. The maximum output power density of the cell module is 0.05 W/cm2 when the temperature of the SiC radiator is 1573 K, while the electrical efficiency of the system is only 0.22%. The efficiency is 1.3% with a Yb203 radiator at the same temperature, however, the maximum output power density drops to 0.03 W/cm2. The values of the open-circuit voltage and the maximum output power obtained from the theoretical model conform to the experimental ones. But the theoretical short-circuit current is higher because of the existence of the contact resistance inside the cell module. In addition, the performance and cost of TPV cogeneration systems with the SiC or Yb203 radiator using industrial high-temperature waste heat were analyzed. The system electrical efficiency could reach 3.1% with a Yb203 radiator at 1573K. The system cost and investment recovery period are 6732 EUR/kWel and 14 years, respectively.

关键词： thermophotovoltaic (TPV) industrial wasteheat ytterbium oxide system efficiency

Adsorption characteristics of droplets applied on non-smooth leaf surface of typical crops

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International Journal of Agricultural and Biological Engineering 2016年第1期9卷 35-41页

作者： wang Shujie wang hujun Li Chun Zhong Xiangmei Huang Hui Zhou Yajun College of Biological and Agricultural Engineering Jilin UniversityChangchun 130025China Key Laboratory of Bionic Engineering(Ministry of Education China)Jilin UniversityChangchun 130025China College of Food Science and Engineering Jilin UniversityChangchun 130025China

To further understand the adsorption characteristics of different-type leaf surfaces adsorbing pesticide droplets and reveal the adsorption mechanism of pesticide droplets on non-smooth leaves,non-smooth leaves of 12 kinds of typical target plants were investigated in this *** parameters of surface morphological characteristics were measured,which include contact angle between leaves and water droplets,water holding capacity surface tension,polar component,dispersion component and other quantitative range of indicators and variation by modern *** relationships between the indicators and water holding capacity were investigated *** experimental results show that the number of trichomes,epidermal wax,morphology and distribution characteristics have influences on adsorption characteristics of the water *** is a negative correlation between free energy of obverse side and the water holding capacity(R=−0.447)while the free energy of reverse side and the water holding capacity show a positive correlation(R=0.212).Also,there is a negative correlation between polar component of obverse side and the water holding capacity(R=−0.357)while the polar component of reverse side and the water holding capacity in plant leaves show a positive correlation(R=0.149).The research can provide a scientific theory for reasonable spraying of pesticide in the agricultural production,and can be a reference for the development of pesticide adjuvants and bionic pesticides.

关键词： surface of plant leaves adsorption characteristics contact angle water holding capacity surface tension

Tribological performance of microstructured surfaces with different wettability from superhydrophilic to superhydrophobic

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Biosurface and Biotribology 2020年第4期6卷 118-123页

作者： Zhihui Zhang Zhenquan Cui hujun wang Chaorui Jiang Jie Zhao Luquan Ren The Key Laboratory of Bionic Engineering of Ministry of Education and the College of Biological and Agricultural Engineering Jilin UniversityChangchun 130022People’s Republic of China State Key Laboratory of Automotive Simulation and Control Jilin UniversityChangchun 130022People’s Republic of China College of Mechanical Engineering Xinjiang UniversityUrumqi 830047People’s Republic of China

The anti-friction function of superwetting surfaces with superhydrophobicity has been ***,the influence regularity of wettability to tribological performance,and the underlying mechanism are still ***,two kinds of microstructured surfaces with different wettability are fabricated on the substrate of steel by controlling surface chemical *** water contact angles on these surfaces range from 0°to 151°.The ball-plate tribological tests are performed under water *** results show that the tribological performance is closely related to surface *** friction coefficient increases with the increase of contact angles when the surfaces are hydrophilic rather than *** contrast,the friction coefficient on the hydrophobic surfaces decreases with the increase of contact ***,the best anti-friction capability is obtained on the superhydrophobic surfaces,and the anti-friction mechanism is *** lowest friction coefficient was 0.12 under the load of 10 *** work provides strong evidence of an association between tribological property and wettability,which may inspire the fabrication and application of special wetting surfaces in friction control.

关键词： friction microstructure steel

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基于高增益观测器的船舶航迹鲁棒跟踪控制

中国造船 2015年第3期56卷 146-153页

作者：王虎军王璐中国劳动关系学院高职学院北京100048 上海交通大学自动化系上海200240

为实现水面船舶的航迹精确跟踪控制,考虑了海浪干扰对控制系统的影响,提出了基于高增益观测器的鲁棒最优控制方法。首先,针对系统内部不确定性和外界扰动对控制精度带来的影响,建立了含有系统复合扰动的非线性航迹跟踪模型;进而,设计高... 详细信息

为实现水面船舶的航迹精确跟踪控制,考虑了海浪干扰对控制系统的影响,提出了基于高增益观测器的鲁棒最优控制方法。首先,针对系统内部不确定性和外界扰动对控制精度带来的影响,建立了含有系统复合扰动的非线性航迹跟踪模型;进而,设计高增益观测器对系统复合扰动进行估计和补偿,并设计一个带有前馈补偿器的最优控制器,获得系统的期望跟踪性能。最后针对某船舶进行了航迹跟踪控制的数值仿真试验,仿真结果表明设计的控制器对外界海浪干扰有较好的抑制作用,可以实现船舶航迹的精确跟踪。

关键词：船舶航迹跟踪高增益观测器最优控制 Lyapunov 稳定性理论

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用于密封液体的一种新型磁性液体密封结构

真空科学与技术学报 2018年第3期38卷 193-198页

作者：王虎军中国劳动关系学院北京100048

磁性液体密封具有"零"泄漏、寿命长等优点,在气体密封等领域得到了成熟的应用,然而,在液体环境中的密封性能较差。本文综述了磁性液体密封液体的发展现状,对传统磁性液体密封结构进行了改进,提出气体隔离式磁性液体密封,将原磁性液体密... 详细信息

磁性液体密封具有"零"泄漏、寿命长等优点,在气体密封等领域得到了成熟的应用,然而,在液体环境中的密封性能较差。本文综述了磁性液体密封液体的发展现状,对传统磁性液体密封结构进行了改进,提出气体隔离式磁性液体密封,将原磁性液体密封液体介质的问题转化为其密封气体介质的问题,从根本上解决了磁性液体与被密封液体界面的不稳定性问题。

关键词：磁性液体密封液体气体隔离密封性能