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Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots

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Horticultural Plant Journal 2024年第2期10卷 450-460页

作者： huiyu wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou State Key Laboratory of Crop Genetics&Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNanjingJiangsu 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNanjingJiangsu 210095China

Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.

关键词： Agrobacterium rhizogenes Non-heading Chinese cabbage Transgenic roots Composite plant Transformation efficiency

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Synthesis of photostable near-infrared sulfone-rhodamines for photoacoustic imaging-guided photothermal therapy

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Chinese Chemical Letters 2024年第3期35卷 369-372页

作者： huiyu Si Dongjuan wang Xianfa Du Xin Zhou Department of Chemistry College of Chemistry and Chemical EngineeringQingdao UniversityQingdao 266071China Department of Orthopedics the Affiliated Hospital of Qingdao UniversityQingdao 266071China

Photothermal therapy(PTT)is a cutting-edge cancer treatment that can kill cancer cells in hypoxic environments without relying on oxygen.Seeking of the ideal photothermal agents with a high absorption coefficient in the near-infrared region,and a high excellent photothermal conversion efficiency is of great significance.Sulfone-Rhodanmine dye has showed an impressive absorption wavelength over 700 nm,but suffered from a stability issue.In this study,we synthesized five sulfone rhodamines and investigated the substitution effects on stability.SO_(2)R2 showed high stability and strong absorbance at 714 nm with an excellent photothermal conversion efficiency of 53.06%,making it suitable for accurate photoacoustic imaging-guided photothermal therapy in vivo.

关键词： Photothermal therapy Sulfone rhodamine Near-Infrared Photoacoustic Imaging

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Monitoring seismicity in the southern Sichuan Basin using a machine learning workflow

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Earthquake Research Advances 2024年第1期4卷 59-66页

作者： Kang wang Jie Zhang Ji Zhang Zhangyu wang huiyu Zhu School of Earth and Space Sciences University of Science and Technology of ChinaHefei230026China

Monitoring seismicity in real time provides significant benefits for timely earthquake warning and analyses.In this study,we propose an automatic workflow based on machine learning(ML)to monitor seismicity in the southern Sichuan Basin of China.This workflow includes coherent event detection,phase picking,and earthquake location using three-component data from a seismic network.By combining Phase Net,we develop an ML-based earthquake location model called Phase Loc,to conduct real-time monitoring of the local seismicity.The approach allows us to use synthetic samples covering the entire study area to train Phase Loc,addressing the problems of insufficient data samples,imbalanced data distribution,and unreliable labels when training with observed data.We apply the trained model to observed data recorded in the southern Sichuan Basin,China,between September 2018 and March 2019.The results show that the average differences in latitude,longitude,and depth are 5.7 km,6.1 km,and 2 km,respectively,compared to the reference catalog.Phase Loc combines all available phase information to make fast and reliable predictions,even if only a few phases are detected and picked.The proposed workflow may help real-time seismic monitoring in other regions as well.

关键词： Earthquake monitoring Machine learning Local seismicity Gaussian waveform Sparse stations

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Caloric restriction, Sirtuins, and cardiovascular diseases

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中华医学杂志(英文版) 2024年第8期137卷 2024 Mar 25页

作者： Wei Ziyu Yang Bo wang huiyu Lv Shuangjie Chen Houzao Liu Depei State Key Laboratory of Common Mechanism Research for Major Diseases Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China

Caloric restriction (CR) is a well-established dietary intervention known to extend healthy lifespan and exert positive effects on aging-related diseases, including cardiovascular conditions. Sirtuins, a family of nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases, have emerged as key regulators of cellular metabolism, stress responses, and the aging process, serving as energy status sensors in response to CR. However, the mechanism through which CR regulates Sirtuin function to ameliorate cardiovascular disease remains unclear. This review not only provided an overview of recent research investigating the interplay between Sirtuins and CR, specifically focusing on their potential implications for cardiovascular health, but also provided a comprehensive summary of the benefits of CR for the cardiovascular system mediated directly via Sirtuins. CR has also been shown to have considerable impact on specific metabolic organs, leading to the production of small molecules that enter systemic circulation and subsequently regulate Sirtuin activity within the cardiovascular system. The direct and indirect effects of CR offer a potential mechanism for Sirtuin modulation and subsequent cardiovascular protection. Understanding the interplay between CR and Sirtuins will provide new insights for the development of interventions to prevent and treat cardiovascular diseases.

关键词： Caloric restriction Sirtuins Cardiovascular diseases Healthy lifestyle Dietary

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Mutation N856K in spike reduces fusogenicity and infectivity of Omicron BA.1

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Signal Transduction and Targeted Therapy 2023年第3期8卷 908-911页

作者： Chunyun Sun huiyu wang Ji Yang Desheng Kong Yuning Chen Haiyue wang Lingling Sun Jianbo Lu Min Teng Liangzhi Xie Beijing Engineering Research Center of Protein and Antibody Sinocelltech Ltd.Beijing 100176China Beijing Key Laboratory of Monoclonal Antibody Research and Development Sino Biological Inc.Beijing 100176China Cell Culture Engineering Center Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100005China

Dear Editor,COVID-19 lung pathology is characterized by interstitial pneumonia with cell-cell fusion-induced syncytia and extensive tissue damage.1 The correlation between viral fusogenicity and pathogenicity has been reported in SARS-CoV-2 variants.2 Compared with the previous Delta or D614G variants,Omicron BA.1 has been proven to be less fusogenic and pathogenic.

关键词： lung pathology damage

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数字技术赋能高校思想政治理论课教学论析

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马克思主义理论学科研究 2024年第3期10卷 29-38页

作者：王易朱惠羽中国人民大学中国人民大学马克思主义学院北京高校思想政治理论课高精尖创新中心北京100872

数字技术赋能高校思想政治理论课教学不是数字技术和高校思想政治理论课的简单相加,而是两者的深度融通。党的十八大以来,特别是学校思想政治理论课教师座谈会召开五年来,数字技术赋能高校思想政治理论课教学取得显著成效。数字技术赋... 详细信息

数字技术赋能高校思想政治理论课教学不是数字技术和高校思想政治理论课的简单相加,而是两者的深度融通。党的十八大以来,特别是学校思想政治理论课教师座谈会召开五年来,数字技术赋能高校思想政治理论课教学取得显著成效。数字技术赋能贯穿高校思想政治理论课教育教学始终,存在着三个关键点需要重点把握和突破:教学场景的数智化打造、教学内容的个性化供给、教学评价的科学化建构。在总结成效、把握关键的同时,也要警惕技术应用的潜在风险,即“算法失当”削弱高校思想政治理论课的价值引领功能、“技术失衡”导致高校思想政治理论课的主体关系异化、“数据失范”威胁高校师生的数据隐私和安全。为有效规避风险,加速推进数字技术赋能高校思想政治理论课教学改革创新,必须把握好“变”与“不变”的前提认知、培育师生数字素养、强化技术运行规范、善用“大思政课”铸魂育人。

关键词：思想政治理论课思想政治理论课教师数字技术赋能

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同质相吸与差异偏好机制驱动的智慧群体结构演化

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系统工程理论与实践 2024年第1期44卷 372-386页

作者：刘鹏王一凡刘惠宇王慧蓉江苏科技大学经济管理学院镇江212100 江苏科技大学深蓝研究院镇江212100

智慧群体所展现出兼具局部聚集性与全局整体性的结构状态引发了学界对其潜在机制的探查.现有主流解释是基于社会资本的结构镶嵌和结构洞机制的组合,对于基于个体属性的同质相吸和差异偏好机制的探讨相对不足.此外,相关工作往往采用多主... 详细信息

智慧群体所展现出兼具局部聚集性与全局整体性的结构状态引发了学界对其潜在机制的探查.现有主流解释是基于社会资本的结构镶嵌和结构洞机制的组合,对于基于个体属性的同质相吸和差异偏好机制的探讨相对不足.此外,相关工作往往采用多主体建模方法,在一定程度上脱离了真实网络且不具备关系形成的预测能力.对此,本文运用数据分析与深度学习相结合的方法,探查了同质相吸和差异偏好机制对于智慧群体结构演化的驱动作用.研究结果表明,本文所考察的两个不同领域智慧群体的协作网络经历三个阶段的演化后(即松散聚簇、链状结构、小世界状态),展现出较好的局部聚集性与全局整体性.其中,同质相吸机制推动相似个体汇聚形成聚簇;差异偏好机制促使聚簇间连接的形成.研究结果不仅有助于进一步认识智慧群体结构演化的内在动力机制,也为复杂网络结构演化机制的分析工作从深度学习视角提供了一个新的思路;同时,对于利用社会智力资源实施开放式创新的管理实践也具有一定的借鉴意义.

关键词：智慧群体结构演化深度学习同质相吸差异偏好

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In-Depth Metaproteomics Analysis of Oral Microbiome for Lung Cancer

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Research 2023年第1期2022卷 313-330页

作者： Xiaoteng Jiang Yan Zhang huiyu wang Zeyuan wang Shen Hu Chengxi Cao Hua Xiao state Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic Developmental SciencesSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghai 200240China School of Pharmacy Shanghai Jiao Tong UniversityShanghai 200240China School of Dentistry and Jonsson Comprehensive Cancer Center University of California-Los AngelesLos Angeles 90095USA Department of Instrument Science and Engineering School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

The human oral microbiome correlates with numerous diseases,including lung cancer.Identifying the functional changes by metaproteomics helps understand the disease-related dysbiosis,yet characterizing low-abundant bacteria is challenging.Here,we developed a free-flow isoelectric focusing electrophoresis-mass spectrometry-(FFIEF-MS-)based metaproteomics strategy to reduce host interferences and enrich low-abundant bacteria for in-depth interpretation of the oral microbiome.With our method,the number of interfering peptides decreased by 52.87%,whereas the bacterial peptides and species increased by 94.97%and 44.90%,respectively,compared to the conventional metaproteomics approach.We identified 3647 bacterial proteins,which is the most comprehensive oral metaproteomics study to date.Lung cancer-associated bacteria were validated among an independent cohort.The imbalanced Fusobacterium nucleatum and Prevotella histicola and their dysregulated functions in inhibiting immune response and maintaining cell redox homeostasis were revealed.The FFIEF-MS may serve as a valuable strategy to study the mechanisms between human diseases and microbiomes with broader applications.

关键词： Lung diseases homeostasis

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中国法律法规中语言权利的历时演进研究:1949—2022

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浙江大学学报（人文社会科学版） 2024年第4期54卷 86-99页

作者：王娅婷张慧玉浙江大学外国语学院/中华译学馆浙江杭州310058

中华人民共和国成立以来,语言权利的保障就备受重视。我国的语言权利保障工作可以分为四个阶段:起步期(1949—1976)、发展期(1977—1996)、完善期(1997—2017)、成熟期(2018年至今)。起步期的相关保障体系还不成熟,主要关注基础领域的... 详细信息

中华人民共和国成立以来,语言权利的保障就备受重视。我国的语言权利保障工作可以分为四个阶段:起步期(1949—1976)、发展期(1977—1996)、完善期(1997—2017)、成熟期(2018年至今)。起步期的相关保障体系还不成熟,主要关注基础领域的语言使用问题;进入到发展期,语言权利保障工作向教育、知识出版、弱势群体语言保障等领域扩展,开始强调语言学习问题;完善期与成熟期的语言权利保障工作涉及领域更广泛,并且开始强调语言的传播,在成熟期尤为重视语言的对外传播。从阶段性发展来看,我国语言权利保障工作涉及领域从基础领域向其他领域扩展,并且工作重心呈现“语言使用—语言学习—语言传播”的转移趋势。从各领域发展特点来看,各领域的语言权利保障工作通常以语言使用为起点,后期更加细化,不仅关注到微观层面的相关职业人员规范、文本规范,还注重结合实际情况对已有条文进行调整,扩展关注群体。

关键词：语言权利语言规划法律法规语言立法

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减税激励、企业投资与资本配置效率——来自小微企业的证据

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南开经济研究 2024年第3期 161-180页

作者：崔惠玉徐颖王宝珠东北财经大学财政税务学院 116025

小微企业是我国社会主义市场经济的重要参与主体,在促进经济发展、改善民生、增加就业等方面具有不可替代的作用。本文基于2010—2018年全国税收调查数据,采用双重差分方法,检验了企业所得税减半征收政策对小微企业投资行为的影响。研... 详细信息

小微企业是我国社会主义市场经济的重要参与主体,在促进经济发展、改善民生、增加就业等方面具有不可替代的作用。本文基于2010—2018年全国税收调查数据,采用双重差分方法,检验了企业所得税减半征收政策对小微企业投资行为的影响。研究表明,所得税减半征收通过增强小微企业的“投资动机”和“投资能力”促进企业固定资产投资的增加,并且所得税减半征收政策对非制造业、未享受政府补助企业和融资约束较强的小微企业投资的促进作用更为明显。进一步分析表明,所得税减半征收政策提升了小微企业的资本配置效率,增强了小微企业吸纳就业的能力,但并未增强小微企业的研发创新能力。总体而言,本文肯定了所得税减半征收政策对小微企业的积极影响,为完善小微企业税收优惠政策、推动经济社会高质量发展提供了有益参考。

关键词：小微企业所得税减半征收企业投资资本配置效率就业影响

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