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Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots

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Horticultural Plant Journal 2024年第2期10卷 450-460页

作者： huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun liu Xilin Hou State Key Laboratory of Crop Genetics&Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNanjingJiangsu 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNanjingJiangsu 210095China

Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin.The one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.

关键词： Agrobacterium rhizogenes Non-heading Chinese cabbage Transgenic roots Composite plant Transformation efficiency

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Modulation of reactive oxygen species to enhance sonodynamic therapy

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Particuology 2023年第4期75卷 199-216页

作者： Xiaoyu liu Xueting Pan Chaohui Wang huiyu liu Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBeijing Laboratory of Biomedical MaterialsBionanomaterials&Translational Engineering LaboratoryBeijing Key Laboratory of BioprocessBeijing University of Chemical TechnologyBeijing100029China

Reactive oxygen species(ROS),involving in many biological reactions,play an important role in disease treatment.Among the various ROS-based therapeutic modalities,sonodynamic therapy(SDT)stands out with its unique advantages.In turn,the SDT efficacy is mainly dependent on the ROS levels in the disease microenvironment.Therefore,in recent years,researchers have extensively investigated SDT with high ROS generation capacity.In this review,we focus on effective strategies to improve the therapeutic ef-ficiency of SDT by modulating ROS,overview the basic mechanisms of ROS generation by sonosensitizers,highlight the rational design of sonosensitizers,and summarize strategies to improve the SDT efficacy by modulating disease microenvironment.In addition,multiple ROS synergistic treatment modalities and the prospect of SDT are discussed.We believe that the understanding and exploration of SDT enhancement strategies will facilitate the clinical translation of SDT.

关键词： Reactive oxygen species Sonodynamic therapy Sonosensitizers Oxidative stress Combination therapy

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Multifunctional mesoporous silica nanoparticles for biomedical applications

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Signal Transduction and Targeted Therapy 2023年第12期8卷 5807-5834页

作者： Bolong Xu Shanshan Li Rui Shi huiyu liu Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBionanomaterials&Translational Engineering LaboratoryBeijing Key Laboratory of BioprocessBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical Technology100029 BeijingChina National Center for Orthopaedics Beijing Research Institute of Traumatology and OrthopaedicsBeijing Jishuitan Hospital100035 BeijingChina

Mesoporous silica nanoparticles(MSNs)are recognized as a prime example of nanotechnology applied in the biomedical field,due to their easily tunable structure and composition,diverse surface functionalization properties,and excellent biocompatibility.Over the past two decades,researchers have developed a wide variety of MSNs-based nanoplatforms through careful design and controlled preparation techniques,demonstrating their adaptability to various biomedical application scenarios.With the continuous breakthroughs of MSNs in the fields of biosensing,disease diagnosis and treatment,tissue engineering,etc.,MSNs are gradually moving from basic research to clinical trials.In this review,we provide a detailed summary of MSNs in the biomedical field,beginning with a comprehensive overview of their development history.We then discuss the types of MSNs-based nanostructured architectures,as well as the classification of MSNs-based nanocomposites according to the elements existed in various inorganic functional components.Subsequently,we summarize the primary purposes of surface-functionalized modifications of MSNs.In the following,we discuss the biomedical applications of MSNs,and highlight the MSNs-based targeted therapeutic modalities currently developed.Given the importance of clinical translation,we also summarize the progress of MSNs in clinical trials.Finally,we take a perspective on the future direction and remaining challenges of MSNs in the biomedical field.

关键词： nanoparticles breakthrough inorganic

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Microwave shock motivating the Sr substitution of 2D porous GdFeO_(3) perovskite for highly active oxygen evolution

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Journal of Energy Chemistry 2024年第1期88卷 232-241,I0006页

作者： Jinglin Xian huiyu Jiang Zhiao Wu Huimin Yu Kaisi liu Miao Fan Rong Hu Guangyu Fang Liyun Wei Jingyan Cai Weilin Xu Huanyu Jin Jun Wan State Key Laboratory of New Textile Materials and Advanced Processing Technologies Hubei Key Laboratory of Biomass Fibers and Eco-Dyeing&FinishingWuhan Textile UniversityWuhan 430200HubeiChina Future Industries Institute STEMUniversity of South AustraliaAdelaideSA 5095Australia Institute for Sustainability Energy and ResourcesThe University of AdelaideAdelaideSA 5005Australia

The incorporation of partial A-site substitution in perovskite oxides represents a promising strategy for precisely controlling the electronic configuration and enhancing its intrinsic catalytic activity.Conventional methods for A-site substitution typically involve prolonged high-temperature processes.While these processes promote the development of unique nanostructures with highly exposed active sites,they often result in the uncontrolled configuration of introduced elements.Herein,we present a novel approach for synthesizing two-dimensional(2D)porous GdFeO_(3) perovskite with A-site strontium(Sr)substitution utilizing microwave shock method.This technique enables precise control of the Sr content and simultaneous construction of 2D porous structures in one step,capitalizing on the advantages of rapid heating and cooling(temperature~1100 K,rate~70 K s^(-1)).The active sites of this oxygen-rich defect structure can be clearly revealed through the simulation of the electronic configuration and the comprehensive analysis of the crystal structure.For electrocatalytic oxygen evolution reaction application,the synthesized 2D porous Gd_(0.8)Sr_(0.2)FeO_(3) electrocatalyst exhibits an exceptional overpotential of 294 mV at a current density of 10 mA cm^(-2)and a small Tafel slope of 55.85 mV dec^(-1)in alkaline electrolytes.This study offers a fresh perspective on designing crystal configurations and the construction of nanostructures in perovskite.

关键词： 2D materials Perovskite Microwave Electrocatalysis Oxygen evolution reaction

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Caloric restriction, Sirtuins, and cardiovascular diseases

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中华医学杂志(英文版) 2024年第8期137卷 2024 Mar 25页

作者： Wei Ziyu Yang Bo Wang huiyu Lv Shuangjie Chen Houzao liu Depei State Key Laboratory of Common Mechanism Research for Major Diseases Department of Biochemistry and Molecular Biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China

Caloric restriction (CR) is a well-established dietary intervention known to extend healthy lifespan and exert positive effects on aging-related diseases, including cardiovascular conditions. Sirtuins, a family of nicotinamide adenine dinucleotide (NAD+)-dependent histone deacetylases, have emerged as key regulators of cellular metabolism, stress responses, and the aging process, serving as energy status sensors in response to CR. However, the mechanism through which CR regulates Sirtuin function to ameliorate cardiovascular disease remains unclear. This review not only provided an overview of recent research investigating the interplay between Sirtuins and CR, specifically focusing on their potential implications for cardiovascular health, but also provided a comprehensive summary of the benefits of CR for the cardiovascular system mediated directly via Sirtuins. CR has also been shown to have considerable impact on specific metabolic organs, leading to the production of small molecules that enter systemic circulation and subsequently regulate Sirtuin activity within the cardiovascular system. The direct and indirect effects of CR offer a potential mechanism for Sirtuin modulation and subsequent cardiovascular protection. Understanding the interplay between CR and Sirtuins will provide new insights for the development of interventions to prevent and treat cardiovascular diseases.

关键词： Caloric restriction Sirtuins Cardiovascular diseases Healthy lifestyle Dietary

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Microwave-assisted exploration of the electron configuration-dependent electrocatalytic urea oxidation activity of 2D porous NiCo_(2)O_(4) spinel

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Journal of Energy Chemistry 2024年第4期91卷 226-235页

作者： Jun Wan Zhiao Wu Guangyu Fang Jinglin Xian Jiao Dai Jiayue Guo Qingxiang Li Yongfei You Kaisi liu Huimin Yu Weilin Xu huiyu Jiang Minggui Xia Huanyu Jin State Key Laboratory of New Textile Materials and Advanced Processing Technologies Hubei Key Laboratory of Biomass Fibers and Eco-Dyeing&FinishingWuhan Textile UniversityWuhan 430200HubeiChina Institute for Sustainability Energy and ResourcesThe University of AdelaideAdelaideSA 5005Australia Future Industries Institute University of South AustraliaMawson Lakes CampusAdelaideSA 5095Australia

Urea holds promise as an alternative water-oxidation substrate in electrolytic cells.High-valence nickelbased spinel,especially after heteroatom doping,excels in urea oxidation reactions(UOR).However,traditional spinel synthesis methods with prolonged high-temperature reactions lack kinetic precision,hindering the balance between controlled doping and highly active two-dimensional(2D)porous structures design.This significantly impedes the identification of electron configuration-dependent active sites in doped 2D nickel-based spinels.Herein,we present a microwave shock method for the preparation of 2D porous NiCo_(2)O_(4)spinel.Utilizing the transient on-off property of microwave pulses for precise heteroatom doping and 2D porous structural design,non-metal doping(boron,phosphorus,and sulfur)with distinct extranuclear electron disparities serves as straightforward examples for investigation.Precise tuning of lattice parameter reveals the impact of covalent bond strength on NiCo_(2)O_(4)structural stability.The introduced defect levels induce unpaired d-electrons in transition metals,enhancing the adsorption of electron-donating amino groups in urea molecules.Simultaneously,Bode plots confirm the impact mechanism of rapid electron migration caused by reduced band gaps on UOR activity.The prepared phosphorus-doped 2D porous NiCo_(2)O_(4),with optimal electron configuration control,outperforms most reported spinels.This controlled modification strategy advances understanding theoretical structure-activity mechanisms of high-performance 2D spinels in UOR.

关键词： 2D materials Spinel Microwave Electrocatalysis Urea oxidation reaction

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Ultrasmall Fe-doped carbon dots nanozymes for photoenhanced antibacterial therapy and wound healing

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Bioactive Materials 2022年第6期7卷 246-256页

作者： Yunhang liu Bolong Xu Mingzhu Lu Shanshan Li Juan Guo Fangzhou Chen Xiaolu Xiong Zhe Yin huiyu liu Dongsheng Zhou Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBionanomaterials&Translational Engineering LaboratoryBeijing Key Laboratory of BioprocessBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing100071China

Pathogenic bacteria pose a devastating threat to public health.However,because of the growing bacterial antibiotic resistance,there is an urgent need to develop alternative antibacterial strategies to the established antibiotics.Herein,iron-doped carbon dots(Fe-CDs,~3 nm)nanozymes with excellent photothermal conversion and photoenhanced enzyme-like properties are developed through a facile one-pot pyrolysis approach for synergistic efficient antibacterial therapy and wound healing.In particular,Fe doping endows CDs with photoenhanced peroxidase(POD)-like activity,which lead to the generation of heat and reactive oxygen species(ROS)for Gram-positive and Gram-negative bacteria killing.This study demonstrates Fe-CDs have significant wound healing efficiency of Fe-CDs by preventing infection,promoting fibroblast proliferation,angiogenesis,and collagen deposition.Furthermore,the ultrasmall size of Fe-CDs possesses good biocompatibility favoring clinical translation.We believe that the nanozyme-mediated therapeutic platform presented here is expected to show promising applications in antibacterial.

关键词： Carbon dots Nanozymes Photothermal effect Antibacterial therapy Wound healing

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A bioactive nanocomposite sponge for simultaneous hemostasis and antimicrobial therapy

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Nano Research 2023年第3期16卷 4004-4012页

作者： Jiani Lei Shanshan Li Shuang liu Qingyuan Wu Bolong Xu Zhijun Huang Nier Wu Xiaolu Xiong huiyu liu Dongsheng Zhou Beijing Advanced Innovation Center for Soft Matter Science and Engineering State Key Laboratory of Organic-Inorganic CompositesBionanomaterials&Translational Engineering LaboratoryBeijing Key Laboratory of BioprocessBeijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing 100029China Beijing National Laboratory of Molecular Sciences Key Laboratory of Green PrintingInstitute of ChemistryChinese Academy of SciencesBeijing 100190China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Uncontrollable bleeding and bacterial infections are the major reasons for the high mortality of post-traumatic.In this study,a composite hemostatic chitosan sponge CaO_(2)@SiO_(2)/CS was prepared by combining a novel core-shell inorganic nano hemostatic CaO_(2)@SiO_(2)nanoparticles with carboxylated chitosan,which presents a multi-layered structure with a rough and hydrophilic surface for rapid absorption of blood.When the CaO_(2)@SiO_(2)nanoparticles in the CaO_(2)@SiO_(2)/CS come into contact with blood,the silanol group on its surface and the released H_(2)O_(2)and Ca2+can recruit and activate platelets,while generating fibrin clots and activating the endo-exogenous coagulation cascade reaction to achieve rapid clotting.The H_(2)O_(2)released from CaO_(2)@SiO_(2)shows the antimicrobial capacity and stimulates the production of tissue factors by endothelial cells.Meanwhile,the silica coating reduces the cytotoxicity of bare CaO_(2),thus reducing the risk of secondary bleeding at the site of vascular injury.CaO_(2)@SiO_(2)/CS(48 s)showed a 1.83-and 2.52-fold reduction in hemostasis time compared to commercial gelfoam and CS in a femoral artery hemorrhage model.This study illustrates the hemostatic mechanism of CaO_(2)@SiO_(2)and provides a reference for the development of clinical biomedical inorganic hemostatic materials.

关键词： calcium peroxide coagulation mechanism composite sponge hemostasis antibacterial

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关系研究与本土传播:一种媒介化的视野

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中州学刊 2024年第3期 169-176页

作者：李红刘慧钰暨南大学新闻与传播学院广东广州510632 暨南大学铸牢中华民族共同体意识研究基地广东广州510632

“关系”概念是一个相对比较成熟的本土化理论成果,它与传播具有天然的亲缘关系,可以援用到传播学研究中。媒介作为基础设施可被视为关系研究的关键变量,以填补既有研究的缺失。在中国,关系并非一个本质化的概念,而是人们处理问题的方法... 详细信息

“关系”概念是一个相对比较成熟的本土化理论成果,它与传播具有天然的亲缘关系,可以援用到传播学研究中。媒介作为基础设施可被视为关系研究的关键变量,以填补既有研究的缺失。在中国,关系并非一个本质化的概念,而是人们处理问题的方法,其内涵充满开放性、经验性和历史感。它可被视为传播学得以本土化的有效通道,而媒介正是其支点。媒介可通过组织时空、培养惯习、重构权力等方式逐步实现对关系形态和关系文化的重塑。因此,传播学本土化可从媒介实践入手,关注媒介对于关系形态及其文化的影响,并将其对接到诸如乡土社会的维系和变迁以及社会治理等议题的讨论中。

关键词：关系研究媒介化本土化

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同质相吸与差异偏好机制驱动的智慧群体结构演化

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系统工程理论与实践 2024年第1期44卷 372-386页

作者：刘鹏王一凡刘惠宇王慧蓉江苏科技大学经济管理学院镇江212100 江苏科技大学深蓝研究院镇江212100

智慧群体所展现出兼具局部聚集性与全局整体性的结构状态引发了学界对其潜在机制的探查.现有主流解释是基于社会资本的结构镶嵌和结构洞机制的组合,对于基于个体属性的同质相吸和差异偏好机制的探讨相对不足.此外,相关工作往往采用多主... 详细信息

智慧群体所展现出兼具局部聚集性与全局整体性的结构状态引发了学界对其潜在机制的探查.现有主流解释是基于社会资本的结构镶嵌和结构洞机制的组合,对于基于个体属性的同质相吸和差异偏好机制的探讨相对不足.此外,相关工作往往采用多主体建模方法,在一定程度上脱离了真实网络且不具备关系形成的预测能力.对此,本文运用数据分析与深度学习相结合的方法,探查了同质相吸和差异偏好机制对于智慧群体结构演化的驱动作用.研究结果表明,本文所考察的两个不同领域智慧群体的协作网络经历三个阶段的演化后(即松散聚簇、链状结构、小世界状态),展现出较好的局部聚集性与全局整体性.其中,同质相吸机制推动相似个体汇聚形成聚簇;差异偏好机制促使聚簇间连接的形成.研究结果不仅有助于进一步认识智慧群体结构演化的内在动力机制,也为复杂网络结构演化机制的分析工作从深度学习视角提供了一个新的思路;同时,对于利用社会智力资源实施开放式创新的管理实践也具有一定的借鉴意义.

关键词：智慧群体结构演化深度学习同质相吸差异偏好

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