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Hepatitis C virus-induced prion protein expression facilitates hepatitis C virus replication

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Virologica Sinica 2017年第6期32卷 503-510页

作者： huixia zhang Shanshan Gao Rongjuan Pei Xinwen Chen Chaoyang Li State Key Laboratory of Virology Center for Molecular Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of the Chinese Academy of Sciences Beijing 100000China

Hepatitis C virus(HCV) infects approximately 180 million people worldwide. Significant progress has been made since the establishment of in vitro HCV infection models in cells. However, the replication of HCV is complex and not completely understood. Here, we found that the expression of host prion protein(Pr P) was induced in an HCV replication cell model. We then showed that increased Pr P expression facilitated HCV genomic replication. Finally, we demonstrated that the KKRPK motif on the N-terminus of Pr P bound nucleic acids and facilitated HCV genomic replication. Our results provided important insights into how viruses may harness cellular protein to achieve propagation.

关键词： hepatitis C virus(HCV) NS3 NS5B prion protein(PrP)

Behaviors analysis of on-coming cluster based on knowledge under cluster security requirements

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Chinese Journal of Aeronautics 2024年第9期37卷 448-462页

作者： huixia zhang Yan LIANG Ying SHI Yuedong WANG Chaoxiong MA Ran WANG School of Automation Northwestern Polytechnical UniversityXi'an 710129China Key Laboratory of Information Fusion Technology Ministry of EducationXi'an 710129China

Analysis of cluster behaviors of the on-coming cluster is an essential measure for high value locations on the *** target tracking and clustering analysis,cluster behaviors analysis under cluster security requirements remains an open issue,which is a joint analysis of clus-tering,intent reasoning and activity *** address this issue,a framework for cluster behav-iors analysis is proposed by incorporating expert knowledge and domain knowledge,and a knowledge-assisted score function with is designed to improve the accuracy of intent reasoning net-work,overcoming the effects of possible knowledge *** framework consists of three mod-ules for cluster analysis,intent reasoning and activity region analysis for typical tasks,in which an intent reasoning network is constructed to obtain cluster intents by using a hybrid knowledge and data driven ***,considering the complexity of the battlefield environment,dif-ferent tasks and corresponding activity region optimization functions are designed for cluster activ-ity region analysis,which are vital elements of cluster behaviors *** demonstrate the effectiveness of the proposed cluster behaviors analysis framework.

关键词： Cluster analysis Intent reasoning,Activity region analysis Cluster behavior Battlefield environment

Complex spatial patterns delay the discovery and location of food by rodents

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Integrative Zoology 2020年第4期15卷 307-313页

作者： Shiqiang zhang Meilin ZHU huixia zhang Nannan YAO Yingying WANG Yifeng zhang Zhenlong WANG School of Life Sciences Zhengzhou UniversityGaoxin DistrictZhengzhouHenan ProvinceChina College of Basic Medical Sciences Zhengzhou UniversityGaoxin DistrictZhengzhouHenan ProvinceChina State-owned Yugong Forest Farm JiyuanHenan ProvinceChina

Spatial patterns in heterogeneity are generally deemed a central causal factor influencing the physiology and behavior of animals in ecological ***,knowledge remains limited about how such patterns influence food discovery by *** inferred that spatial heterogeneity plays a key role in animal food discovery and *** prove this inference,we tested food locating parameters by 2 rodent species,Apodemus agrarius and Lasiopodomys brandtii,in different heterogeneous *** results showed that spatial heterogeneity significantly influenced the food locating time of rodents,with food locating time increasing with increasing spatial ***,spatial heterogeneity significantly influenced invalid excavations(digging in the wrong place).Finally,spatial heterogeneity significantly influenced the frequency that heterogeneous objects were *** our inference,our results indicate that spatial heterogeneity significantly influences the foraging behavior of ***,increased spatial heterogeneity will impair the food locating success of *** believe that this work will broaden our understanding of plant–animal interactions.

关键词： food locating invalid excavation rodents spatial heterogeneity

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无头轧制过程中工作辊热辊形的研究

中国重型装备 2025年第1期 11-14,21页

作者：马博张慧霞辛绍贺一重集团大连工程技术有限公司辽宁大连116000

基于交替差分的方法建立无头轧制温度场及热变形计算模型,用于实际生产中轧辊热辊形的预报,并结合某轧制计划,计算了轧制过程中工作辊的温度场与热辊形,计算结果与理论分析一致。模型计算结果还可以用于对轧辊冷却水的分析,即对水量、... 详细信息

基于交替差分的方法建立无头轧制温度场及热变形计算模型,用于实际生产中轧辊热辊形的预报,并结合某轧制计划,计算了轧制过程中工作辊的温度场与热辊形,计算结果与理论分析一致。模型计算结果还可以用于对轧辊冷却水的分析,即对水量、温度和沿辊身不同部位所需冷却水的准确需求进行预测,为轧辊冷却水集管的布置形式和控制方式的优化提供理论依据。

关键词：换热系数温度场无头轧制

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Emerging tropane alkaloids:Global development and potential health threats

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Food Quality and Safety 2024年第1期8卷 12-26页

作者： Zile Wang Shiqi Chen Pimiao Zheng Zhenhui Ren huixia zhang Jing zhang Bing Shao Congming Wu Haiyang Jiang Department of Veterinary Pharmacology and Toxicology Beijing Key Laboratory of Detection Technology for Animal-Derived Food SafetyBeijing Laboratory for Food Quality and SafetyNational Key Laboratory of Veterinary Public Health and SafetyCollege of Veterinary MedicineChina Agricultural UniversityBeijingChina. College of Veterinary Medicine Shandong Agricultural UniversityTaianChina. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry College of Animal Science and Veterinary MedicineTianjin Agricultural UniversityTianjinChina Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning Beijing Center for Disease Prevention and ControlBeijingChina.

Tropane alkaloids(TAs)are a kind of plant secondary metabolite that mainly originate from *** have potent anticholinergic activity,and are well-known anticholinergic drugs,but have also been reported as plant *** studies have been conducted on TAs,but no scientometric research has been *** study aimed to expound the knowledge network and development of the feld of TAs and predict the emerging development topics based on *** particular,this research combined VOSviewer and CiteSpace for visualization and covered 1,298 related scientifc publications(1953–2022).The research on TAs is rapidly developing,with the participation of 86 countries,1,129 research institutions,and 4,087 ***,the University of Geneva,and *** are the most productive country,institution,and researcher,***,the main research topics on TAs include biosynthesis,in vitro synthesis,chemical synthesis,and determination *** 2018,the monitoring of TAs in food related to human health has shown citation burst characteristics,which is considered an emerging development trend in the *** fndings of this study will enable researchers to quickly tap into the knowledge background/structure of TAs from massive data to provide a reference for further research.

关键词： Tropane alkaloids bibliometrics biosynthesis in vitro synthesis chemical synthesis determination contamination food safety

MTBSTFA derivatization-LC-MS/MS approach for the quantitative analysis of endogenous nucleotides in human colorectal carcinoma cells

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Journal of Pharmaceutical Analysis 2022年第1期12卷 77-86页

作者： huixia zhang Yan Li Zheng Li Christopher Wai-Kei Lam Peng Zhu Caiyun Wang Hua Zhou Wei zhang State Key Laboratory of Quality Research in Chinese Medicines Macao Institute for Applied Research in Medicine and HealthMacao University of Science and TechnologyTaipaMacaoChina

Endogenous ribonucleotides(RNs)and deoxyribonucleotides(dRNs)are important metabolites related to the pathogenesis of many *** light of their physiological and pathological significances,a novel and sensitive pre-column derivatization method with N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide(MTBSTFA)was developed to determine RNs and dRNs in human cells using high-performance liquid chromatography tandem mass spectrometry(HPLC-MS/MS).A one-step extraction of cells with 85%methanol followed by a simple derivatization reaction within 5 min at room temperature contributed to shortened analysis *** derivatives of 22 nucleoside mono-,di-and tri-phosphates were retained on the typical C;column and eluted by ammonium acetate and acetonitrile in 9 *** these optimal conditions,good linearity was achieved in the tested calibration *** lower limit of quantitation(LLOQ)was determined to be 0.1-0.4μM for the tested RNs and 0.001-0.1μM for *** addition,the precision(CV)was<15%and the RSD of stability was lower than 10.4%.Furthermore,this method was applied to quantify the endogenous nucleotides in human colorectal carcinoma cell lines HCT 116 exposed to *** conclusion,our method has proven to be simple,rapid,sensitive,and *** may be used for specific expanded studies on intracellular pharmacology in vitro.

关键词： Ribonucleotides Deoxyribonucleotides Derivatization N-(t-butyldimethylsilyl)-N-methyltrifluoroacetamide High-performance liquid chromatography tandem mass spectrometry

PRRSV degrades MDA5 via dual autophagy receptors P62 and CCT2 to evade antiviral innate immunity

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Virologica Sinica 2024年第2期39卷 264-276页

作者： Ruiqi Sun Yanyu Guo Lilin zhang huixia zhang Boxuan Yin Xiaoyang Li Changyan Li Liu Yang Lei zhang Zexing Li Jinhai Huang School of Life Sciences Tianjin UniversityTianjin300072China

Porcine reproductive and respiratory syndrome virus(PRRSV)is a major economically devastating pathogen that has evolved various strategies to evade innate *** of antiviral interferon largely promotes PRRSV immunoevasion by utilizing cytoplasmic melanoma differentiation-associated gene 5(MDA5),a receptor that senses viral *** this study,the downregulated transcription and expression levels of porcine MDA5 in PRRSV infection were observed,and the detailed mechanisms were *** found that the interaction between P62 and MDA5 is enhanced due to two factors:the phosphorylation modification of the autophagic receptor P62 by the upregulated kinase CK2αand the K63 ubiquitination of porcine MDA5 catalyzed by the E3 ubiquitinase TRIM21 in PRRSV-infected *** a result of these modifications,the classic P62-mediated autophagy is ***,porcine MDA5 interacts with the chaperonin containing TCP1 subunit 2(CCT2),which is enhanced by PRRSV *** interaction promotes the aggregate formation and autophagic clearance of MDA5-CCT2-nsp3 independently of *** summary,enhanced MDA5 degradation occurs in PRRSV infection via two autophagic pathways:the binding of MDA5 with the autophagy receptor P62 and the aggrephagy receptor CCT2,leading to intense innate immune *** research reveals a novel mechanism of immune evasion in PRRSV infection and provides fundamental insights for the development of new vaccines or therapeutic strategies.

关键词： Porcine reproductive and respiratory syndrome virus(PRRSV) nsp3 Autophagy MDA5

Recursive Nonlinear Filtering with Event-Triggered Data Transmission and Random Time Delays 36

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Recursive Nonlinear Filtering with Event-Triggered Data Tran...

Structure-tissue exposure/selectivity relationship(STR) correlates with clinical efficacy/safety

第36届中国控制会议

作者： Dongdong Yu Li Li Mengfei Niu huixia zhang Cui Zhu School of Electrical Engineering Yanshan University School of Information and Communication Engineering Beijing Information Science and Technology University

ISBN: (纸本)9781538629185

This paper concentrates on the nonlinear filtering for systems with event-triggered data transmission and random time delays.A stochastic event-triggered sensor schedule is introduced to reduce the excessive measurements,which achieves a lower communication rate with a good estimation *** time delay is modeled by a Poisson distribution,and a-length buffer is used to retrieve partly delayed ***,a nonlinear filter is designed for the state estimation with event-triggered data transmission and random time *** example is provided to verify the performance of the proposed algorithm.

关键词： Unscented Kalman filter Nonlinear systems Stochastic event-triggered sensor schedule Time delays

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Acta Pharmaceutica Sinica B 2022年第5期12卷 2462-2478页

作者： Wei Gao Hongxiang Hu Lipeng Dai Miao He Hebao Yuan huixia zhang Jinhui Liao Bo Wen Yan Li Maria Palmisano Mohamed Dit Mady Traore Simon Zhou Duxin Sun Department of Pharmaceutical Sciences College of PharmacyUniversity of MichiganAnn ArborMI 48109USA Translational Development and Clinical Pharmacology Bristol Myers SquibbSummitNJ 07920USA

Drug optimization, which improves drug potency/specificity by structure-activity relationship(SAR) and drug-like properties, is rigorously performed to select drug candidates for clinical trials. However,the current drug optimization may overlook the structure-tissue exposure/selectivity-relationship(STR) in disease-targeted tissues vs. normal tissues, which may mislead the drug candidate selection and impact the balance of clinical efficacy/toxicity. In this study, we investigated the STR in correlation with observed clinical efficacy/toxicity using seven selective estrogen receptor modulators(SERMs) that have similar structures, same molecular target, and similar/different pharmacokinetics. The results showed that drug’s plasma exposure was not correlated with drug’s exposures in the target tissues(tumor, fat pad, bone, uterus),while tissue exposure/selectivity of SERMs was correlated with clinical efficacy/safety. Slight structure modifications of four SERMs did not change drug’s plasma exposure but altered drug’s tissue exposure/*** SERMs with high protein binding showed higher accumulation in tumors compared to surrounding normal tissues, which is likely due to tumor EPR effect of protein-bound drugs. These suggest that STR alters drug’s tissue exposure/selectivity in disease-targeted tissues vs. normal tissues impacting clinical efficacy/toxicity. Drug optimization needs to balance the SAR and STR in selecting drug candidate for clinical trial to improve success of clinical drug development.

关键词： Structure-activity-relationship(SAR) Structure-tissue exposure/selectivity relationship(STR) Drug optimization Clinical efficacy/toxicity Drug development