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Effects of different concentrations of TiAl6V4 particles on MC3T3-E1 cells and bone in rats

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Medicine in Novel Technology and Devices 2020年第3期7卷 8-12页

作者： huiqin luan Zhanyong Yu Jian Li Jingfang Bi Zhongjun Mo Weiyan Ren Zengyong Li Key Laboratory of Intelligent Control and Rehabilitation Technology of the Ministry of Civil Affairs National Research Center for Rehabilitation Technical AidsBeijing100176China Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability Beijing100176China Key Laboratory of Rehabilitation Technical Aids Analysis and Identification of the Ministry of Civil Affairs Beijing100176China The Second Hospital of Tangshan HebeiChina Neurology Taian City Central HospitalTaianShandongChina

Three-dimensional(3D)-printed porous Ti6Al4V implants have good mechanical properties and excellent biocompatibility.As such,these implants are widely used in orthopedics.Particles adhere between the sintered and nonsintered interfaces of the porous samples during 3D printing.These excess particles can be cleaned by blowing the particles and via ultrasound,but the excess internal particles of complex structural parts are difficult to remove.During long-term cyclic loading,stress and strain can cause residual Ti6Al4V particles in the pores of the implant to shed.These detached Ti6Al4V particles are in extensive contact with osteoblasts and scattered around the implant.In this study,we examined the effects of different concentrations of Ti6Al4V particles on osteoblasts and bones.MC3T3-E1 cells were used to evaluate the effects of different concentrations of Ti6Al4V particles on cells after 72 h on the basis of the expression levels of genes,involving osteopontin,alkaline phosphatase,bone morphogenetic protein-2 and runt-related transcription factor-2.Microtubule-associated protein 1 light chain 3 was used to detect the autophagy of MC3T3-E1 with different concentrations of Ti6Al4V particles.The distal femoral defects of rats were examined to examine bone growth with different concentrations of Ti6Al4V particles.All rats were accepted by micro-CT and biochemical analyses after 12 weeks.The results indicated that 10 and 100μg/ml of Ti6Al4V particles may improve osteogenic differentiation.Micro-CT revealed that low concentrations of Ti6Al4V particles may improve the osteogenesis of the rats.However,the(cortical and trabecular)BMD of middle and high dose groups was no significant change compared with control group.In conclusion,low-dose residual particles do not inhibit osteoblast differentiation and do not decrease the bone mineral density of rats.

关键词： Three-dimensional printing Titanium alloy particles Osteogenesis Bone defect Bone mineral density Biochemical parameters

The bone alterations in hind limb amputation rats in vivo

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Medicine in Novel Technology and Devices 2020年第4期8卷 31-35页

作者： huiqin luan Huiru gu Zhongjun Mo Weiyan Ren Huan Guo Zhaowei Chu Yubo Fan Key Laboratory of Intelligent Control and Rehabilitation Technology of the Ministry of Civil Affairs National Research Center for Rehabilitation Technical AidsBeijing100176China Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability Beijing100176China Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical EngineeringBeihang UniversityBeijing100191China Key Laboratory of Rehabilitation Technical Aids Analysis and Identification of the Ministry of Civil Affairs Beijing100176China

Osteoporosis,which is characterized by bone loss,increases the risk of fractures and s and seriously affected the amputee’s health.Muscle mass is an important factor affecting bone mineral density(BMD).Muscular atrophy and decreased muscle mass can cause BMD to fall.The bone quantity and quality are used to assess by Microcomputer tomography(micro-CT)with non-destructive.To investigate the performance of micro-CT in evaluating amputation-induced osteopenia in rats,the micro-CT was used to evaluate the change of the microstructure in trabecular bone and cortical bone of amputation.Muscle morphology was analyzed by micro-CT and transmission electron microscopy.Female Sprague–Dawley rats(8 week old)were divided into two groups:control(CON)groups and left hind limb amputation(LHLA).At 35 days,microstructure and volumetric bone mineral density(vBMD)of the tibia and femur were detected through micro-CT.The results showed:(1)the trabecular and cortical vBMD of the proximal tibia and distal femur in the LHLA group were significantly lower than those in the CON group.(2)Compared with the CON group,the trabecular number,bone volume fraction and trabecular thickness of proximal tibia and distal femur in LHLA group decreased significantly,whereas the trabecular separation,structure model index and bone surface/bone volume increased significantly in LHLA group.(3)The whole cross-sectional area(CSA)of whole calf muscles of left limb in LHLA group decreased significantly compared with CON group.In general,hind limb amputation could cause bone loss,changes in the microstructure of trabecular bone and muscle atrophy.Micro-CT is sensitive in evaluating bone microstructure destruction caused by amputation,and can be used for early diagnosis or monitor the development of bone loss in amputation in vivo.

关键词： Amputation Micro-CT Microstructure Bone loss BMD

The effect of pore size and porosity of Ti6Al4V scaffolds on MC3T3-E1 cells and tissue in rabbits

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Science China(Technological Sciences) 2019年第7期62卷 1160-1168页

作者： luan huiqin WANG LiTing REN WeiYan CHU Zhao Wei HUANG YunFei LU ChengLin FAN YuBo Key Laboratory of Intelligent Control and Rehabilitation Technology of the Ministry of Civil Affairs National Research Center for Rehabilitation Technical Aids Beijing 100176 China Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability Beijing 100176 China Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical Engineering Beihang University Beijing 100191 China Shandong Weigao Orthopedic Device Company Limited Weihai 264209 China

Electron beam melting(EBM) allows the fabrication of specific porous titanium implants, whereas their in vitro and in vivo biological performance should be further investigated. In this study, we examined the porous Ti6 Al4 V scaffolds(low, 334.1 μm pore size with 55.4% porosity;middle, 383.2 μm pore size with 65.2% porosity;and high, 401.6 μm pore size with 78.1%porosity) fabricated through EBM. The structural characterization and mechanical properties of porous Ti6 Al4 V scaffolds were measured through micro-computed tomography(micro-CT), scanning electron microscopy, and a material testing system.MC3 T3-E1 cells were used to assess the proliferation and differentiation of the cells on different scaffolds at day 7 and day 14 based on the expression levels of genes, including alkaline phosphatase, bone morphogenetic protein-2, osteopontin and runtrelated transcription factor-2. Rabbits with distal femoral defects were utilized to evaluate bone ingrowth in the porous titanium.All of the samples were subjected to micro-CT and histological analysis after 12 weeks. Results showed that compressive Young’s modulus of 0.3–1.1 GPa was similar to the trabecular bone. The three types of porous Ti6 Al4 V scaffolds were inclined to promote cell proliferation, whereas cell differentiation and bone ingrowth into the porous scaffolds were biased to the porous titanium with relatively large pores and porosity(middle and high). This study implied that the present porous implant design,which had the combined advantages of different pore sizes and porosity, might be meaningful and promising for trabecular bone defect restoration.

关键词： pore size porosity porous titanium alloy scaffolds osteogenesis bone defect

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假肢接受腔内衬套的细胞毒性及力学性能检测

中国康复理论与实践 2022年第4期28卷 479-483页

作者：栾会芹毕经芳吴赛男任韦燕刘红梅张莹莹李增勇国家康复辅具研究中心北京市100176 国家康复辅具研究中心康复辅具质量监督检验中心北京市100176 民政部康复辅具分析评定重点实验室北京市100176 山东省泰安市中心医院山东泰安市271000 国家康复辅具研究中心附属康复医院北京市100176

目的调查研究国内市售假肢接受腔内衬套的细胞毒性及力学性能现状。方法收集国产厚度6 mm泡沫内衬(A)、国产厚度5 mm EVA泡沫内衬(B)、德国产厚度5 mm EVA泡沫内衬(C)、德国产厚度12 mm PE泡沫内衬套(D)、冰岛产厚度3 mm硅胶内衬套(E)... 详细信息

目的调查研究国内市售假肢接受腔内衬套的细胞毒性及力学性能现状。方法收集国产厚度6 mm泡沫内衬(A)、国产厚度5 mm EVA泡沫内衬(B)、德国产厚度5 mm EVA泡沫内衬(C)、德国产厚度12 mm PE泡沫内衬套(D)、冰岛产厚度3 mm硅胶内衬套(E)、德国产厚度4 mm凝胶内衬套(F)。应用显微镜观察与噻唑蓝比色法,进行不同内衬套细胞毒性检测。采用高锰酸钾消耗测定有机小分子物质含量。采用材料力学试验机对内衬套材料进行拉伸强度、扯断伸长率(%)、100%定伸强度的检测。采用邵氏硬度计进行硬度测试。结果内衬套A、B、C和D的细胞毒性反应均为2级,内衬套E和F的细胞毒性反应均为0级。内衬套A、B、C高锰酸钾消耗量均超过150 mg/kg。除内衬套C外,其余5种产品硬度均≤70 HA。除内衬套D外,其余5种产品均拉伸强度>1 MPa,断裂伸长率>120.0%,100%定伸强度>0.9 MPa。结论因材料和生产工艺等原因6种样品的细胞毒性和力学性能有较大差异。

关键词：假肢接受腔内衬套细胞毒性力学性能

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被动运动对抗尾吊大鼠软骨退化的效果

航天医学与医学工程 2014年第3期27卷 172-175页

作者：栾会芹黄云飞樊瑜波孙联文北京航空航天大学生物与医学工程学院生物力学与力生物学教育部重点实验室北京100191

目的探寻后肢去负荷过程中,能够阻止软骨退化的对抗措施。方法 SD大鼠分3组(每组8只):对照组(Con)、尾吊组(TS)、尾吊+被动运动组(TSP)。尾吊21天期间,TSP组接受由训练装置提供的被动运动训练。利用μCT与组织切片检测大鼠股骨远端软骨... 详细信息

目的探寻后肢去负荷过程中,能够阻止软骨退化的对抗措施。方法 SD大鼠分3组(每组8只):对照组(Con)、尾吊组(TS)、尾吊+被动运动组(TSP)。尾吊21天期间,TSP组接受由训练装置提供的被动运动训练。利用μCT与组织切片检测大鼠股骨远端软骨的变化。结果μCT结果表明,TS与TSP组大鼠股骨远端的关节软骨厚度与Con比明显下降;TS的软骨体积较Con与TSP显著下降,而Con与TSP间没有显著差异;TS与TSP的软骨的衰减系数较Con明显升高。组织切片表明,TS与TSP的软骨厚度较Con明显下降,番红O—固绿染色显示TS与TSP的基质染色程度较Con下降,软骨细胞出现减少的现象。此外,μCT所测厚度与组织切片所测厚度高度相关。结论大鼠尾吊会导致关节软骨退化,然而被动运动不能有效的阻止尾吊引起的软骨退化。

关键词：模拟失重被动运动关节软骨软骨退化 μCT

高效提取富含多糖多酚的果实组织中总RNA的方法

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食品科学 2009年第13期30卷 29-32页

作者：栾福磊韩友生邵毅卢丞文陈安钧李玲田慧琴罗云波朱本忠中国农业大学食品科学与营养工程学院北京10083

果实组织中往往富含多糖和多酚,由此导致了果实组织比其他植物组织总RNA的提取要困难的多。本研究以番茄为模式材料,探索自制TRIZOL试剂法从富含多糖的果实组织中提取总RNA。以葡萄为模式材料探索PVP改进法提取富含多酚的果实组织的总RN... 详细信息

果实组织中往往富含多糖和多酚,由此导致了果实组织比其他植物组织总RNA的提取要困难的多。本研究以番茄为模式材料,探索自制TRIZOL试剂法从富含多糖的果实组织中提取总RNA。以葡萄为模式材料探索PVP改进法提取富含多酚的果实组织的总RNA的方法。经过纯度、得率检测证明所研究的两种新方法能够保证从富含多糖和多酚的果实组织中获得高得率和高质量的总RNA。

关键词：果实组织总RNA TRIZOL PVP