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Why mosaic? Gene expression profiling of African cassava mosaic virus-infected cassava reveals the effect of chlorophyll degradation on symptom development

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Journal of Integrative Plant biology 2014年第2期56卷 122-132页

作者： Jiao Liu Jun Yang huiping bi Peng Zhang Shanghai Chenshan Plant Science Research Center the Chinese Academy of Sciences Chenshan Botanical Garden National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research (Shanghai) Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences the Chinese Academy of Science Key Laboratory of Systems Microbial biotechnology Tianjin Institute of Industrial Biotechnology the Chinese Academy of Sciences Tianjin Airport Economic Park

Cassava mosaic disease, caused by cassava bego- moviruses, is the most serious disease for cassava in Africa. However, the pathogenesis of this disease is poorly under- stood. We employed high throughput digital gene expression profiling based on the Illumina Solexa sequencing technology to investigate the global transcriptional response of cassava to African cassava mosaic virus infection. We found that 3,21o genes were differentially expressed in virus-infected cassava leaves. Gene ontology term and Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that genes implicated in photosynthesis were most affected, consistent with the chlorotic symptoms observed in infected leaves. The upregu- lation of chlorophyll degradation genes, including the genes encoding chlorophyUase, pheophytinase, and pheophorbide a oxygenase, and downregulation of genes encoding the major apoproteins in light-harvesting complex II were confirmed by qRT-PCR. These findings, together with the reduction of chlorophyll b content and fewer grana stacks in the infected leaf cells, reveal that the degradation of chlorophyll plays an important role in A^rican cassava mosaic virus symptom development. This study will provide a road map for future investigations into viral pathogenesis.

关键词： Afriican cassava mosaic virus cassava chlorophylldegradation digital gene expression mosaic symptom

A Controllable Synthetic Route for Preparing Graphene-Cu and Graphene-Cu_2O Nanocomposites Using Graphene Oxide-CuO as a Precursor

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Journal of Wuhan University of Technology(Materials Science) 2015年第5期30卷 947-950页

作者：陈丽朱俊武 bi huiping MENG Xiaoqian YAO Pengcheng HAN Qiaofeng Key Laboratory for Soft Chemistry and Functional Materials Nanjing University of Science and Technology Ministry of Education School of Materials Science and Engineering Tianjin University

The development of convenient method to obtain graphene-based nanocomposites is a key issue for their application. Herein, we described a facile route for synthesizing graphene-Cu and graphene-Cu2 O nanocomposites using graphene oxide-Cu O as a precursor. Remarkably, the different nanocomposites could be formed just by varying the reaction temperature and time. This work provides a feasible route for the preparation of graphene-based nanocomposites with various constituents.

关键词： graphene-based nanocomposites various constituents precursor

Rod-like bi_(4)O_(5)I_(2)/bi_(4)O_(5)Br_(2) step-scheme heterostructure with oxygen vacancies synthesized by calcining the solid solution containing organic group

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Chinese Journal of Catalysis 2022年第2期43卷 288-302页

作者： Xuemei Jia Zichen Shen Qiaofeng Han huiping bi Key Laboratory for Soft Chemistry and Functional Materials Ministry of EducationNanjing University of Science and TechnologyNanjing 210094JiangsuChina

To improve separation efficiency of the photogenerated electron-hole pairs,constructing a heterojunction is considered to be a promising ***,the fabrication of heterojunction via a facile route to achieve a substantial improvement in photocatalytic performance is still *** this work,a well-designed nanosheet-based rodlike step-scheme(S-scheme)heterojunction bi_(4)O_(5)I_(2)/bi_(4)O_(5)Br_(2) with rich oxygen vacancies(OVs)(bi_(4)O_(5)I_(2)/bi_(4)O_(5)Br_(2)-OV)was easily synthesized by calcining biOAc0.6Br0.2I0.2(Ac-=CH3 COO-)*** as-prepared bi4O5I2/bi4O5Br2-OV exhibited excellent visible light photocatalytic performance towards antibiotic tetracycline(TC)and dye rhodamine B(Rh B)degradation and removal rate reached 90.2% and 97.0%within 120 min,respectively,which was higher than those of bi4O5I2-OV(56.8% and 71.8%),bi4O5Br2-OV(47.4%and 68.4%),solid solution biOAc0.6Br0.2I0.2(67.0% and 84.0%)and bi_(4)O_(5)I_(2)/bi_(4)O_(5)Br_(2) with poor oxygen vacancies(bi4O5I2/bi4O5Br2-P)(30.6%and 40.4%).Owing to the release of heat and generation of reducing carbon during calcining the precursor with Ac-,it could not only reduce the generation temperature of bi-rich bismuth oxyhalides,which thus decreased particle size and increased surface areas,but also introduce surface OVs,which could trap photoelectrons and inhibit the recombination of *** addition,the calcination of single solid solution precursor benefited to the formation of well-alloyed interfaces with larger contact areas between 2D/2D nanosheet-like materials,which facilitates charge carriers transfer at the *** bi4O5I2/bi4O5Br2-OV also shows the desirable removal rate for TC and Rh B in actual wastewater or in the presence of some *** study provides an effective and simple strategy for designing OVs modified bi-rich oxyhalides heterojunctions.

关键词： Calcination Organic group-based solid solution Phase transformation Oxygen vacancy S-scheme heterojunction Photocatalysis

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大肠杆菌混菌发酵生产咖啡醇糖苷

食品与发酵工业 2020年第23期46卷 7-13页

作者：王帅庄以彬刘浩毕慧萍刘涛天津科技大学生物工程学院天津300457 中国科学院天津工业生物技术研究所天津300308

利用大肠杆菌混菌发酵生物合成咖啡醇糖苷。通过在酪氨酸高产大肠杆菌BTAL中表达异源基因酪氨酸解氨酶(tyrosine ammonia-lyase from Rhodotorula glutinis,RgTAL),对羟基肉桂酰辅酶A连接酶(hydroxycinnamate:CoA ligase from Petroselinum crispum,Pc4CL)和肉桂酰辅酶A还原酶(cinnamyl-CoA reductase from Arabidopsis thaliana,AtCCR),与大肠杆菌BL21(DE3)中表达对羟基苯乙酸3-羟化酶HpaBC和糖基转移酶(UGT73C5 from ***,AtUGT73C5)混合培养发酵生产了3种咖啡醇葡萄糖苷,其中2种为非天然化合物。优化共培养比例至3∶1,咖啡醇-4-O-葡萄糖苷的最高产量达到(141.63±3.42)mg/L。这是首次微生物异源合成咖啡醇糖苷的报道,该工作扩大了咖啡醇糖苷的结构多样性,为后续的构效分析提供了新的候选化合物,有望得到具有较好生物活性的咖啡醇糖苷化合物。

关键词：咖啡醇糖苷生物合成大肠杆菌混菌培养发酵生产

Genetic Engineering of Cassava and Sweet Potato for Industrial Applications

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3个植物源苯甲醇酰基转移酶合成乙酸肉桂酯的研究

2010全国植物生物学研讨会

作者： Jun Yang Shanshan Zhao Dong An huiping bi Jia Xu Qiuxiang Ma Min Zhang Jia Liu Peng Zhang Institute of Plant Physiology & Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences

Cassava(Manihot esculenta Crantz)and sweetpotato(Ipomoea batatas)accumulate a lot of starch in their storage roots which can be processed to food,feed and *** high yield potential and robustness against unfavorable en... 详细信息

关键词： Cassava sweetpotato genetic improvement transgenesis industrialization

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微生物学通报 2021年第7期48卷 2365-2373页

作者：胡田东殷华毕慧萍刘浩庄以彬刘涛天津科技大学生物工程学院天津300457 中国科学院天津工业生物技术研究所天津300308

[背景]乙酸肉桂酯是一种重要的香料化合物,在化妆品和食品工业上具有广泛的应用,传统的生产方法主要依靠植物提取和化学合成。[目的]通过筛选不同植物源的酰基转移酶,利用大肠杆菌从头合成乙酸肉桂酯。[方法]首先,通过在苯丙氨酸高产菌B... 详细信息

[背景]乙酸肉桂酯是一种重要的香料化合物,在化妆品和食品工业上具有广泛的应用,传统的生产方法主要依靠植物提取和化学合成。[目的]通过筛选不同植物源的酰基转移酶,利用大肠杆菌从头合成乙酸肉桂酯。[方法]首先,通过在苯丙氨酸高产菌BPHE中表达异源基因苯丙氨酸解氨酶(Phenylalanine Ammonia-Lyase from Arabidopsis thaliana,AtPAL)、对羟基肉桂酰辅酶A连接酶(Hydroxycinnamate:CoA Ligase from Petroselinum crispum,Pc4CL)和肉桂酰辅酶A还原酶(Cinnamyl-CoA Reductase from Arabidopsis thaliana,AtCCR),并结合大肠杆菌自身的内源性醇脱氢酶(Alcohol Dehydrogenases,ADHs)或醛酮还原酶(Aldo-Keto Reductases,AKRs)的催化作用构建了从苯丙氨酸到肉桂醇的生物合成途径。然后,苯甲醇苯甲酰转移酶(Benzyl Alcohol O-Benzoyltransferase from Nicotiana tabacum,ANN09798;Benzyl Alcohol O-Benzoyltransferase from Clarkia breweri,ANN09796)或苯甲醇乙酰转移酶(Benzyl Alcohol Acetyltransferase from Clarkia breweri,BEAT)被引入到上述重组大肠杆菌中发酵培养生产乙酸肉桂酯。最后,在大肠杆菌中过表达乙酰辅酶A合成酶(Acetyl Coenzyme A Synthetase,ACS)来提高底物乙酰辅酶A的量。[结果]探讨了3个植物源苯甲醇酰基转移酶生物合成乙酸肉桂酯的能力,并应用于合成乙酸肉桂酯的细胞工厂,最终使乙酸肉桂酯最高产量达到166.9±6.6mg/L。[结论]植物源苯甲醇酰基转移酶具有一定的底物宽泛性,能以肉桂醇为底物催化合成乙酸肉桂酯。首次利用植物源的苯甲醇酰基转移酶合成乙酸肉桂酯,为微生物细胞工厂以葡萄糖作为碳源生产乙酸肉桂酯提供参考。

关键词：乙酸肉桂酯生物合成大肠杆菌苯甲醇酰基转移酶发酵生产

具有不同自由离子的离子交换膜对MFC性能的影响

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水处理技术 2016年第8期42卷 69-73,78页

作者：麻继臻耿慧毕慧平胡朝霞陈守文南京理工大学环境与生物工程学院江苏南京210094

将4种具有不同自由离子的离子交换膜,即阳离子交换膜的H+型(CEM-H)和Na+型(CEM-Na)、及阴离子交换膜的Cl-型(AEM-Cl)和OH-型(AEM-OH),作为分离膜应用于微生物燃料电池(MFC)。结果表明,4种膜的电导率呈顺序为CEM-H>CEM-Na>AEM-OH>AEM-Cl... 详细信息

将4种具有不同自由离子的离子交换膜,即阳离子交换膜的H+型(CEM-H)和Na+型(CEM-Na)、及阴离子交换膜的Cl-型(AEM-Cl)和OH-型(AEM-OH),作为分离膜应用于微生物燃料电池(MFC)。结果表明,4种膜的电导率呈顺序为CEM-H>CEM-Na>AEM-OH>AEM-Cl,与之相对应的MFC产电性能呈相同的顺序。采用CEM-H的MFC产电性能最好,最大功率密度可达899 m W/m2;同时膜性能的不同导致MFC的库伦效率、阳极p H等存在差异。电化学阻抗谱(EIS)研究表明,在电池运行过程中阴离子交换膜的污染造成了较大的内阻,严重影响了其MFC的产电性能。

关键词：微生物燃料电池离子交换膜膜污染

Genetic Engineering of Cassava and Sweet Potato for Industrial Applications

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磺化聚芳醚砜/磺化聚酰亚胺复合质子交换膜的制备与性能研究

植物分子生物学与现代农业——2010全国植物生物学研讨会

作者： Jun Yang,Shanshan Zhao,Dong An,huiping bi,Jia Xu,Qiuxiang Ma,Min Zhang, Jia Liu,Peng Zhang~* Institute of Plant Physiology & Ecology,Shanghai Institutes for biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China

Cassava(Manihot esculenta Crantz) and sweetpotato(Ipomoea batatas) accumulate a lot of starch in their storage roots which can be processed to food,feed and bio-ethanol. The high yield potential and robustness against unfavorable environmental conditions make them the suitable energy crops for margin land cultivation,which will not highly compete with other food crops on arable *** are several key biological

关键词： Cassava sweetpotato genetic improvement transgenesis industrialization

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高分子学报 2010年第8期20卷 966-972页

作者：毕慧平王佳力陈守文胡朝霞高智琳张轩王连军冈本健一南京理工大学化工学院南京210094 山口大学工学部日本宇部755-8611

利用溶液浇铸法制备了一系列双磺化型磺化聚芳醚砜/磺化聚酰亚胺(SPAES/SPI)复合质子交换膜.扫描电子显微镜(SEM)结果显示复合膜不存在明显的相分离,表明二者具有很好的相容性.由于SPI的引入,复合膜在甲醇中稳定性较纯SPAES具有大幅的提... 详细信息

利用溶液浇铸法制备了一系列双磺化型磺化聚芳醚砜/磺化聚酰亚胺(SPAES/SPI)复合质子交换膜.扫描电子显微镜(SEM)结果显示复合膜不存在明显的相分离,表明二者具有很好的相容性.由于SPI的引入,复合膜在甲醇中稳定性较纯SPAES具有大幅的提高,比Nafion112低得多的甲醇吸收率表明了这些复合膜具有比后者更低的甲醇透过率.复合膜显示了与单组分膜相类似的高温分解稳定性,磺酸基团的分解温度达到了290℃以上.复合膜显示出远高于纯SPAES膜的尺寸稳定性能,在130℃高温中200h处理后,所有的复合膜均保持了高的机械性能,而此时纯SPAES膜已经溶解于水中.而且由于两种磺化聚合物间的复合,复合膜维持了较高的IEC水平,显示了较高的质子导电率,在80%相对湿度时的质子导电率与Nafion112相近,而在水中的质子导电率均高于Nafion112.

关键词：复合质子交换膜磺化聚芳醚砜磺化聚酰亚胺燃料电池