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基于色谱-质谱联用技术检测尿液中类固醇激素及代谢物的研究进展

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环境科学研究 2024年第6期37卷 1363-1377页

作者：马晓查玉娥刘娟任惠敏赵力常红唐宋山东大学公共卫生学院山东济南250061 中国疾病预防控制中心环境与人群健康重点实验室中国疾病预防控制中心环境与健康相关产品安全所北京100021 北京林业大学环境科学与工程学院北京100083 南京医科大学公共卫生学院全球健康中心江苏南京211166

类固醇激素在人体新陈代谢、免疫调节、生长发育、维持生命功能等方面具有重要作用,但其同时又作为一类新型有机污染物,干扰人体正常的内分泌机能从而对人体产生危害,因此类固醇激素的研究已逐渐成为国际上环境和健康领域的研究热点。... 详细信息

类固醇激素在人体新陈代谢、免疫调节、生长发育、维持生命功能等方面具有重要作用,但其同时又作为一类新型有机污染物,干扰人体正常的内分泌机能从而对人体产生危害,因此类固醇激素的研究已逐渐成为国际上环境和健康领域的研究热点。尿液具有无创收集、前处理简单、能敏感地反映生理变化等优势,更适用于定期、重复监测。色谱-质谱联用技术灵敏度高、选择性好、通量高,在尿液类固醇激素检测中得到了广泛应用。本文基于色谱-质谱联用技术综述了检测类固醇激素的尿样的采集和储存条件、前处理技术、色谱-质谱分析方法、分析软件和数据库,以及部分类固醇激素在我国不同健康人群尿液中的检出情况等研究进展,指出尿液中类固醇激素及其代谢物的检测基质复杂、种类繁多以及含量较低,仍需进行广泛而深入的研究,实现对多种类固醇激素及其代谢物的精确定量仍面临挑战。未来研究需建立标准化采集和储存方法,开发更高效和环境友好型的前处理技术以及高灵敏度、高可靠性和高通量的色谱-质谱仪器,合成更多类固醇激素及代谢物标准品和同位素,同时不断完善数据库信息以满足精确定量的需求,为尿液中类固醇激素检测及人群健康风险评估等研究提供参考。

关键词：尿液类固醇激素代谢物前处理技术色谱-质谱分析

Molecular mechanisms governing plant responses to high temperatures

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Journal of Integrative Plant Biology 2018年第9期60卷 757-779页

作者： Bingjie Li Kang Gao huimin ren Wenqiang Tang Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Collaboration Innovation Center for Cell SignalingHebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang 050024China

The increased prevalence of high temperatures （HTs） around the world is a major global concern, as they dramatically affect agronomic productivity. Upon HT exposure, plants sense the temperature change and initiate cellular and metabolic responses that enable them to adapt to their new environmental *** the mechanisms by which plants cope with HT will facilitate the development of molecular markers to enable the production of plants with improved thermotolerance. In recent decades, genetic, physiological, molecular, and biochemical studies have revealed a number of vital cellular components and processes involved in thermoresponsive growth and the acquisition of thermo- tolerance in plants. This review summarizes the major mechanisms involved in plant HT responses, with a special focus on recent discoveries related to plant thermosensing, heat stress signaling, and HT-regulated gene expression networks that promote plant adaptation to elevated environmental temperatures.

关键词： Molecular mechanisms governing plant responses to high temperatures HT CDPKs ZIP

校园英语文化建设背景下高职院校英语社团作用研究与探索

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英语教师 2024年第12期24卷 21-23,32页

作者：于浩任惠敏王梦蝶山东药品食品职业学院山东威海264210

分析高职院校校园英语文化建设的现状,提出校园英语文化作为高职院校教育的重要方面,在英语教学的具体实施过程中往往存在与英语学科教学联动不明晰、对教育主体培养认知不具体、整体文化导向单一等问题。阐述高职院校英语社团的重要性... 详细信息

分析高职院校校园英语文化建设的现状,提出校园英语文化作为高职院校教育的重要方面,在英语教学的具体实施过程中往往存在与英语学科教学联动不明晰、对教育主体培养认知不具体、整体文化导向单一等问题。阐述高职院校英语社团的重要性及建设作用,并从丰富学生组织框架、耦合第一第二课堂、发挥校园的精神建设场地作用等三个方面进行高职院校英语社团在校园英语文化建设过程中的实践探索。认为作为校园英语文化建设的重要组织框架载体,英语社团发挥着重要作用,能以“润物细无声”的方式对学生进行思想教育。

关键词：高职院校校园文化校园英语文化英语社团

毛竹SLAC家族基因鉴定及PheSLAC1功能分析

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园艺学报 2024年第3期51卷 545-559页

作者：刘晋红王峥于昊辛依睿亓果宁柳参奎任慧敏浙江农林大学林业与生物技术学院亚热带森林培育国家重点实验室杭州311300

为了研究S型阴离子通道蛋白(slow type anion channel,SLAC)基因家族在毛竹(Phyllostachys edulis)响应干旱胁迫中的作用,利用生物信息方法以拟南芥和水稻中的SLAC/SLAH家族蛋白序列为模板对毛竹中SLAC家族成员进行鉴定,对其基因结构、... 详细信息

为了研究S型阴离子通道蛋白(slow type anion channel,SLAC)基因家族在毛竹(Phyllostachys edulis)响应干旱胁迫中的作用,利用生物信息方法以拟南芥和水稻中的SLAC/SLAH家族蛋白序列为模板对毛竹中SLAC家族成员进行鉴定,对其基因结构、进化关系和组织表达等进行分析,并对PheSLAC1的保守功能位点以及在干旱胁迫中的功能进行初步研究。结果表明:毛竹中包含15个SLAC家族基因,其中PheSLAC1.1(PH02Gene01933.t1)和Phe SLAC1.2(PH02Gene00592.t1)在叶片中表达量最高,基因编码区(coding sequence,CDS)序列长度分别为1 716和1 677 bp,分别编码572和559个氨基酸,且包含保守的关键功能位点。亚细胞定位结果表明,PheSLAC1.1和Phe SLAC1.2均定位于细胞质膜中。PheSLAC1.1和Phe SLAC1.2在拟南芥slac1-3突变体中的表达结果表明,其能够部分减轻突变体的干旱敏感表型,表明两者均能够通过调控气孔关闭在毛竹响应干旱胁迫中发挥重要作用。

关键词：毛竹 SLAC基因家族干旱胁迫功能鉴定

High Fluorescence Porous Organic Cage for Sensing Divalent Palladium Ion and Encapsulating Fine Palladium Nanoparticles

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Chinese Journal of Chemistry 2022年第3期40卷 385-391页

作者： huimin ren Chao Liu Xu Ding Xianzhang Fu Hailong Wang Jianzhuang Jiang Beijing Advanced Innovation Center for Materials Genome Engineering Beijing Key Laboratory for Science and Application of Functional Molecular and Crystalline MaterialsDepartment of Chemistry and Chemical EngineeringSchool of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijing 100083China

Porous organic cages(POCs)as an innovative type of porous molecular materials enable multifunctional ***,a fluorescence POC(denoted as 1)has been constructed by means of 5/5'-((2/5-dimethoxy-l/4-phenylene)bis(ethyne-2/1-diyl))diiso-phthalaldehyde condensing with cyclohexanediamine enantiomer with the aid of trifluoroacetic acid.1 exhibits the permanent void and prominent fluorescence with relative quantum yield of 73%confirmed by gas sorption and emission experiments,***,this organic cage is capable of sensing diavalent Pd2+according to the fluorescence response to the addition of various metal *** dispersion of 1 in methanol containing palladium acetate was stirred at 25℃and then 80℃to reduce Pd2+ions into nanoparticles(NPs),leading to the composite(Pd@l)composed of fine Pd NPs with the size of co.1.8 *** catalytic nature of Pd@l over NaBH4 promoted the degeneration of nitrobenzene and congo *** work introduces one new case of POCs with versatile properties and functions,including ion fluorescence recognition,fine Pd NPs stabilizer,and gas adsorption,enriching the family of POC-based materials.

关键词： Catalysis Fluorescence recognition Nanoparticles Cage compounds Palladium

The carbon isotopic compositions of Non-methane Hydrocarbons in atmosphere

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Chinese Science Bulletin 2009年第8期54卷 1422-1425页

作者： PENG Lin ZHANG huimin ren ZhaoFang MU Ling SHI RuiLiang CHANG LiPing LI Fan Key Laboratory of Coal Science and Technology (Taiyuan University of Technology) Ministry of Education and Shanxi ProvinceTaiyuan 030024 China

Carbon isotopic compositions of atmospheric Non-methane Hydrocarbons(NMHCs) in the urban areas of Taiyuan and Lanzhou in summer were reported and the sources of NMHCs are *** isotopic ratios(δ 13C) of vehicle exhaust,coal-combustion exhaust,fuel volatiles and cooking exhaust were also measured with thermal desorption-gas chromatography-isotope ratio-mass spectrometry(TD-GC-IR-MS).δ 13C values of NMHCs in the urban areas of Lanzhou and Taiyuan range from-32.3‰ to-22.3‰ and from-32.8‰ to-18.1‰.δ 13C values of vehicle exhaust,coal-combustion exhaust,fuel volatiles and cooking exhaust are-32.6‰―-21.7‰,-24.5‰―-22.3‰,-32.5‰―-27.4‰ and-31.6‰―-24.5‰,*** data indicate that vehicle exhaust and cooking exhaust make a significant contribution to the atmospheric ***,to reduce emissions of vehicle exhaust and cook-ing exhaust is critical for controlling atmospheric NMHCs pollution in summer.

关键词：非甲烷碳氢化合物碳同位素组成碳同位素比值燃煤废气机动车尾气气氛大气污染城市地区

浅埋滴灌条件下氮肥基追比对甜菜生长、产量和含糖率的影响

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作物杂志 2024年第3期 186-191页

作者：李智郭晓霞黄春燕菅彩媛田露韩康任霄云任惠敏张鹏刘佳孔德娟王珍珍苏文斌内蒙古自治区农牧业科学院特色作物研究所内蒙古呼和浩特010031 乌兰察布市农林科学研究所内蒙古集宁012000

于2021-2022年连续2年研究浅埋滴灌条件下不同氮肥基追比对甜菜叶片厚度、单位长度内的叶脉数量、株高、叶面积指数(LAI)、根冠比、单株干物质量、产量和含糖率的影响。结果表明,随氮肥追肥比例的增加,含糖率、叶片厚度和根冠比呈降低... 详细信息

于2021-2022年连续2年研究浅埋滴灌条件下不同氮肥基追比对甜菜叶片厚度、单位长度内的叶脉数量、株高、叶面积指数(LAI)、根冠比、单株干物质量、产量和含糖率的影响。结果表明,随氮肥追肥比例的增加,含糖率、叶片厚度和根冠比呈降低的趋势,而产量、产糖量、株高、叶面积指数和单株干物质积累量显著增加,N3处理(氮肥按基追比6:4)比CK处理(氮肥全部基施)产量提高6.93%~8.04%、产糖量提高6.12%~8.94%。相关性分析表明,甜菜产量与株高、LAI、单株干物质量呈显著正相关关系,而与叶片厚度呈显著负相关关系。因此,浅埋滴灌条件下,氮肥按基追比6:4有利于甜菜产量和产糖量的提高。

关键词：甜菜浅埋滴灌生长指标产量含糖率

Super-resolution imaging for in situ monitoring sub-cellular micro-dynamics of small molecule drug

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1864-1877页

作者： huimin Chen Guiqian Fang Youxiao ren Weiwei Zou Kang Ying Zhiwei Yang Qixin Chen School of Pharmaceutical Sciences&Institute of Materia Medica Medical Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesJinan 250062China MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter School of PhysicsXi'an Jiaotong UniversityXi'an 710049China Reproductive Medicine Center Department of Obstetrics and Gynecologythe First Affiliated Hospital of Anhui Medical UniversityHefei 230022China

Small molecule drugs play a pivotal role in the arsenal of anticancer pharmacological agents. Nonetheless, their small size poses a challenge when directly visualizing their localization, distribution, mechanism of action (MOA), and target engagement at the subcellular level in real time. We propose a strategy for developing triple-functioning drug beacons that seamlessly integrate therapeutically relevant bioactivity, precise subcellular localization, and direct visualization capabilities within a single molecular entity. As a proof of concept, we have meticulously designed and constructed a boronic acid fluorescence drug beacon using coumarin–hemicyanine (CHB). Our CHB design includes three pivotal features: a boronic acid moiety that binds both adenosine triphosphate (ATP) and adenosine diphosphate (ADP), thus depleting their levels and disrupting the energy supply within mitochondria;a positively charged component that targets the drug beacon to mitochondria;and a sizeable conjugated luminophore that emits fluorescence, facilitating the application of structured illumination microscopy (SIM). Our study indicates the exceptional responsiveness of our proof-of-concept drug beacon to ADP and ATP, its efficacy in inhibiting tumor growth, and its ability to facilitate the tracking of ADP and ATP distribution around the mitochondrial cristae. Furthermore, our investigation reveals that the micro-dynamics of CHB induce mitochondrial dysfunction by causing damage to the mitochondrial cristae and mitochondrial DNA. Altogether, our findings highlight the potential of SIM in conjunction with visual drug design as a potent tool for monitoring the in situ MOA of small molecule anticancer compounds. This approach represents a crucial advancement in addressing a current challenge within the field of small molecule drug discovery and validation.

关键词： Mitochondrial Drug beacon Sub-cellular Small molecule Energy supply Anti-tumor

竹红素A联合LED红光照射通过YAP途径抑制瘢痕疙瘩成纤维细胞增殖

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安徽医学 2024年第2期45卷 142-146页

作者：陈雷刚任慧敏吴远慧安国芝景晓蕾赵同心河北北方学院附属第一医院皮肤科河北张家口075000 河北北方学院附属第一医院急诊科河北张家口075000 河北北方学院研究生院河北张家口075000

目的探究hypocrellin A(HA)联合LED红光照射通过Yes相关蛋白(YAP)途径抑制瘢痕疙瘩成纤维细胞(KFs)增殖的作用及机制。方法原代培养KFs并进行传代,第3代KFs分为对照组、H组(1.0μmol/L HA处理)、L组(3 J/cm~2红光照射)、H+L组、阴性对照... 详细信息

目的探究hypocrellin A(HA)联合LED红光照射通过Yes相关蛋白(YAP)途径抑制瘢痕疙瘩成纤维细胞(KFs)增殖的作用及机制。方法原代培养KFs并进行传代,第3代KFs分为对照组、H组(1.0μmol/L HA处理)、L组(3 J/cm~2红光照射)、H+L组、阴性对照(NC)质粒组、NC质粒+H+L组、YAP质粒+H+L组、检测细胞增殖的OD_(450)水平及BrdU阳性率,氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的含量,YAP、β-连环蛋白(β-catenin)的表达水平。结果H组、L组、H+L组KFs的OD_(450)、BrdU阳性率、SOD及GSH-Px的含量、YAP及β-catenin的表达水平均低于对照组,MDA的含量高于对照组(P<0.05),且H+L组上述指标的变化较H组、L组更明显(P<0.05);YAP质粒+H+L组KFs的OD_(450)、BrdU阳性率、SOD及GSH-Px的含量、YAP及β-catenin的表达水平均高于NC质粒+H+L组,MDA的含量低于质粒+H+L组(P<0.05)。结论HA联合LED红光照射通过抑制YAP/β-catenin途径抑制KFs增殖、激活KFs氧化应激。

关键词：瘢痕疙瘩成纤维细胞竹红素A 增殖氧化应激 Yes相关蛋白