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GhWRKY33 negatively regulates jasmonate-mediated plant defense to Verticillium dahliae

Plant Diversity 2023年第3期45卷 337-346页

作者： Yunrui Ji Minghui Mou huimin zhang Ruling Wang Songguo Wu Yifen Jing Haiyan zhang Lanxin Li Zhifang Li Ligang Chen CAS Key Laboratory of Tropical Plant Resources and Sustainable Use Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMenglunMenglaYunnan 666303China Center of Economic Botany Core Botanical GardensChinese Academy of SciencesMenglunMenglaYunnan 666303China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences Henan UniversityKaifeng 475004China

Verticillium wilt,caused by Verticillium dahliae,seriously restricts the yield and quality improvement of cotton.Previous studies have revealed the involvement of WRKY members in plant defense against V.dahliae,but the underlying mechanisms involved need to be further elucidated.Here,we demonstrated that Gossypium hirsutum WRKY DNA-binding protein 33(GhWRKY33) functions as a negative regulator in plant defense against V.dahliae.GhWRKY33 expression is induced rapidly by V.dahliae and methyl jasmonate,and overexpression of GhWRKY33 reduces plant tolerance to V.dahliae in Arabidopsis.Quantitative RT-PCR analysis revealed that expression of several JA-associated genes was significantly repressed in GhWRKY33 overexpressing transgenic plants.Yeast one-hybrid analysis revealed that GhWRKY33 may repress the transcription of both AtERF1 and GhERF2 through its binding to their promoters.Protein-protein interaction analysis suggested that GhWRKY33 interacts with G.hirsutum JASMONATE ZIM-domain protein 3(GhJAZ3).Similarly,overexpression of GhJAZ3 also decreases plant tolerance to V.dahliae.Furthermore,GhJAZ3 acts synergistically with GhWRKY33 to suppress both AtERF1 and GhERF2 expression.Our results imply that GhWRKY33 may negatively regulate plant tolerance to V.dahliae via the JA-mediated signaling pathway.

关键词： GhWRKY33 Verticillium dahliae Jasmonate acid GhJAZ3 Defense response

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Design of SiNridge-slot micro-ring resonator and generation of dual-comb based on orthogonally bicolor pumping

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Optoelectronics Letters 2024年

作者： Wen Jin Wang Qian Yu huimin Wu Zhengwei zhang Hui School of Science Xi'an Shiyou University Shaanxi Engineering Research Center of Oil and Gas Resource Optical Fiber Detection Shaanxi Key Laboratory of Measurement and Control Technology for Oil and Gas wells

A novel high quality-factor （Q） micro-ring resonator （MRR） structure based on the Si3N4ridge-slot waveguide is proposed， and the MRR is pumped by orthogonally polarized bicolor pumping to generate dual-comb. We optimized the structure of MRR by the finite element method and precise dispersion engineering， which finally obtained the suitable MRR geometry with negative dispersion characteristics at 1550 nm， having Q of 1.7×107and the absorption loss as low as 2.6×10-5dB/cm. The simulation model of generating dual-comb is established as coupled Lugiato-Lefever equation （LLE）， which takes into account the higher order dispersion， cross-phase modulation （XPM）， multiphoton absorption， and external pumping. Solved by the split-step Fourier method （SSFM） and the fourth-order Runge-Kutta （RK4） method， the numerical results show that the generated dual-comb is periodically equally spaced distribution but with slightly different intensities in the time domain. In the frequency domain， there are 64 comb teeth with intensities higher than -100 dBm with a bandwidth of 120 nm. Particularly， in the case of bicolor orthogonal polarization pumping， a smaller amount of detuning does not greatly affect the bandwidth of the dual-comb.

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Micro-Service-Based Radio Access Network

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China Communications 2022年第3期19卷 1-15页

作者： Na Li Guangyi Liu huimin zhang Quan Zhao Yun Zhao Zhou Tong Yingying Wang Junshuai Sun Future Research Lab China Mobile Research InstituteBeijing 100053China

The convergence of information,communication,and data technologies(ICDT)has been identified as one of the developing trends of the sixth generation(6G)network.Service-based architecture(SBA)as one of the promising information technology,has been preliminarily introduced into the fifth generation(5G)core network(CN)and successfully commercialized,which verifies its feasibility and effectiveness.However,SBA mainly focuses on the control plane of CN at present and the SBA-CN user plane is being studied by the industry.In addition to further evolving the SBA-CN,SBA radio access network(RAN)should also be considered to enable an end-toend SBA,so as to meet more comprehensive and extreme requirements of future applications,as well as support fast rollout requirements of RAN devices.

关键词： 6G micro-service SBA-RAN

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Iron-doped Ag/Ni_(2)(CO_(3))(OH)_(2)hierarchical microtubes for highly efficient water oxidation

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Carbon Energy 2022年第5期4卷 939-949页

作者： Huiwen zhang Shuxuan Liu Enlai Hu Yunfei Yang huimin zhang Yuting Zhu Lijing Yan Xuehui Gao Jing zhang Zhan Lin Key Laboratory of the Ministry of Education for Advanced Catalysis Materials Department of ChemistryZhejiang Normal UniversityJinhuaChina College of Materials and Chemistry China Jiliang UniversityHangzhouChina Guangzhou Key Laboratory of Clean Transportation Energy Chemistry Guangzhou Higher Education Mega CenterGuangdong University of TechnologyGuangzhouGuangdongChina

Doping of foreign atoms and construction of unique structures are considered as effective approaches to design high-activity and strongdurability electrocatalysts.Herein,we report Fe-doped nickel hydroxide carbonate hierarchical microtubes with Ag nanoparticles(denoted Ag/NiFeHC HMTs)through hydrolysis precipitation process.Experimental tests and density functional theory calculations reveal that Fe doping can tune the electron configuration to enhance the conductivity,markedly improve the electrochemical surface area to expose more active sites,and act as reactive centers to lower the free energy of the rate determination step.In addition,the unique hierarchical structure can also offer active sites and excellent cycling stability.Benefitting from these advantages,the as-obtained Ag/NiFeHC HMTs show excellent oxygen evolution reaction activity,with an overpotential of 208 mV at 10 mA cm^(−2)in 1.0M KOH.Also,it could achieve long-term stability at a current density of 20 mA cm^(−2)for 24 h.

关键词： density functional theory Fe doping Ni2(CO3)(OH)2 hierarchical microtubes water oxidation reaction

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A droplet‐based electricity generator with ultrahigh instantaneous output and short charging time

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Droplet 2022年第1期1卷 56-64页

作者： Nan zhang huimin zhang Wanghuai Xu Haojie Gu Shimeng Ye Huanxi Zheng Yuxin Song Zuankai Wang Xiaofeng Zhou Shanghai Key Laboratory of Multidimensional Information Processing School of Communication and Electronic EngineeringEast China Normal UniversityShanghaiChina Department of Mechanical Engineering City University of Hong KongHong KongChina

The past several years have witnessed the rapid development in effectively transforming randomly distributed water kinetic energy into electrical energy,especially triggered by the emergence of droplet‐based electricity generators(DEG).Despite this,it still suffers from relatively low average power density,which is also achieved at the cost of long charging time,the time to reach stable and saturated surface charge density either through continuous droplet impingement or precharging.Although the harvested energy per droplet in DEG remains as the dominant metric,ultrahigh instantaneous output and short charging time are equally important in some specialized applications such as instantaneous luminescence.Here,we conduct systematical modeling and optimization to build the link between the hydrodynamic and electrical systems,which enables us to determine ultrahigh instantaneous output and short charging time by tailoring parameters such as dielectric layer thickness,droplet ion concentration,and external load.We envision that this strategy in achieving ultrahigh instantaneous output as well as shortening charging time would provide insights and design routes for water energy harvesting.

关键词： specialized charging generator

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Diatomic active sites nanozymes:Enhanced peroxidase-like activity for dopamine and intracellular H2O2 detection

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Nano Research 2022年第5期15卷 4266-4273页

作者： Shan Wang Zunfu Hu Qiulian Wei huimin zhang Weina Tang Yunqiang Sun Haiqiang Duan Zhichao Dai Qingyun Liu Xiuwen Zheng Key Laboratory of Functional Nanomaterials and Technology in Universities of Shandong College of Chemistry and Chemical EngineeringLinyi UniversityLinyi 276000China School of Chemical and Biological Engineering Shandong University of Science and TechnologyQingdao 266510China School of Materials Science and Engineering Linyi UniversityLinyi 276000China

Considering intracellular hydrogen peroxide(H_(2)O_(2))plays pivotal roles in the regulation of serial biological processes,the in-situ detection of intracellular H2O2 has attracted an extensive attention.In the present work,an atomically dispersed diatomic active sites Nanozymes(FeN_(3)/PtN_(4)-single-atom nanozymes(SAzyme))was prepared exhibiting enhanced peroxidase-like activity.The obvious synergistic effect between Fe-Pt heteronuclear diatomic active sites was confirmed by series of characterization and density functional theory(DFT).The peroxidase-like activity of Fe-sites could be substantially enhanced by the bonded Pt-sites via the modulation effect.As a consequence,the gap between the d-band centre(εd)of Fe 3d orbitals and the Fermi energy level was narrowed and the electronic interaction could be strengthened,leading to a lower free energy barrier and a lower activation energy as well as fortified metal-O bonding in the kinetic pathway.Therefore,the constructed FeN3/PtN4-SAzyme exhibited higher peroxidase-like activity than that of FeN4-SAzyme.The FeN3/PtN4-SAzyme-assisted oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)facilitated the colorimetric detection of dopamine(DA),an important biomolecule.The linear detection range and limit of detection(LOD)of DA and H_(2)O_(2) were 1-10 μM,0.01-1.0 mM and 0.109 μM,7.97 μM,respectively.In addition,the constructed SAzymes were also applied for the in-situ detection of intracellular H2O2,expanding the application scope of the newborn SAzymes.

关键词： single-atom nanozymes heteronuclear diatomic active sites peroxidase-like dopamine intracellular H_(2)O_(2)

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A three-parameter single-step time integration method for structural dynamic analysis

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Acta Mechanica Sinica 2019年第1期35卷 112-128,I0004页

作者： huimin zhang Yufeng Xing Institute of Solid Mechanics Beihang University (BUAA)Beijing 100083China

The existing three-parameter single-step time integration methods, such as the Generalized-a method, improve numerical dissipation by modifying equilibrium equation at time points, which cause them to lose accuracy due to the interpolation of load vectors. Moreover, these three-parameter methods do not present an available formulation applied to a general secondorder non linear differential equatio n. To solve these problems, this paper proposes an innovative three-parameter single-step method by introducing an additional variable into update equations. Although the present method is spectrally identical to the Generalized-cx method for undamped systems, it possesses higher accuracy since it strictly satisfies the equilibrium equation at time points, and can be readily used to solve nonlinear equations. By the analysis of accuracy, stability, numerical dissipation and dispersion, the optimal second-order implicit and explicit schemes are generated, which can maximize low-frequency accuracy when high-frequency dissipation is specified. To check the performance of the proposed method, several numerical experiments are conducted and the proposed method is compared with a few up-to-date methods.

关键词： Three-parameter Single-step Optimal scheme Higher accuracy

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NKT cells in liver diseases

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Frontiers of Medicine 2018年第3期12卷 249-261页

作者： Shasha Zhu huimin zhang Li Bai CAS Key Laboratory of Innate Immunity and Chronic Disease CAS Center for Excellence in Molecular Cell Science School of Life Sciences and Medical Center University of Science and Technology of China Hefei 230027 China Innovation Center for Cell Signaling Network Hefei National Laboratory for Physical Sciences at Microscale Hefei 230027 China

Natural killer T cells are innate-like and tissue-resident lymphocytes, which recognize lipid antigens and are enriched in the liver. Natural killer T cells play important roles in infections, tumors, autoimmune diseases, and metabolic diseases. In this study, we summarize recent findings on biology of natural killer T cells and their roles in hepatitis B virus and hepatitis C virus infection, autoimmune liver diseases, alcoholic liver disease, nonalcoholic fatty liver disease, and hepatocellular carcinoma. Controversial results from previous studies are discussed, and indicate the dynamic alteration in the role of natural killer T cells during the progression of liver diseases, which might be caused by changes in natural killer T subsets, factors skewing cytokine responses, and intercellular crosstalk between natural killer T cells and CDld-expressing cells or bystander cells.

关键词： natural killer T cells hepatitis B virus and hepatitis C virus infection autoimmune liver diseases alcoholic liverdisease nonalcoholic fatty liver disease hepatocellular carcinoma

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A genomic association study revealing subphenotypes of childhood steroid-sensitive nephrotic syndrome in a larger genomic sequencing cohort

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Genes & Diseases 2024年第4期11卷 400-410页

作者： Han Chan Fenfen Ni Bo Zhao huimin Jiang Juanjuan Ding Li Wang Xiaowen Wang Jingjing Cui Shipin Feng Xiaojie Gao Xueying Yang Huan Chi Hao Lee Xuelan Chen Xiaoqin Li Jia Jiao Daoqi Wu Gaofu zhang Mo Wang Yupeng Cun Xiongzhong Ruan Haiping Yang Qiu Li Department of Nephrology Children's Hospital of Chongqing Medical UniversityNational Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of PediatricsChongqing 400014China Department of Nephrology Shenzhen Children's HospitalShenzhenGuangdong 518034China Department of Nephrology Kunming Children's HospitalKunming Medical UniversityKunmingYunnan 650228China Department of Nephrology Wuhan Children's HospitalTongji Medical College of Huazhong University of Science and TechnologyWuhanHubei 430015China Department of Nephrology Chengdu Women and Children Central HospitalChengduSichuan 610073China Pediatric Research Institute Ministry of Education Key Laboratory of Child Development and DisordersNational Clinical Research Center for Child Health and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory DisordersChildren's Hospital of Chongqing Medical UniversityChongqing 400014China Department of Nephrology John Moorhead Research LaboratoryUniversity College London Medical SchoolRoyal Free CampusUniversity College LondonLondon NW32PFUnited Kingdom

Dissecting the genetic components that contribute to the two main subphenotypes of steroid-sensitive nephrotic syndrome(SSNS)using genome-wide association studies(GWAS)strategy is important for understanding the disease.We conducted a multicenter cohort study(360 patients and 1835 controls)combined with a GWAS strategy to identify susceptibility var-iants associated with the following two subphenotypes of ssNS:steroid-sensitive nephrotic syn-drome without relapse(SSNswR,181 patients)and steroid-dependent/frequent relapse nephrotic syndrome(SDNS/FRNS,179 patients).The distribution of two single-nucleotide poly-morphisms(SNPs)in ANKRD36 and ALPG was significant between SSNSWR and healthy controls,and that of two SNPs in GAD1 and HLA-DQA1 was significant between SDNS/FRNS and healthy controls.Interestingly,rs1047989 in HLA-DQA1 was a candidate locus for SDNS/FRNS but not for SSNSWR.No significant SNPs were observed between SSNSWR and SDNS/FRNS.Meanwhile,chromosome 2:171713702 in GAD1 was associated with a greater steroid dose(>0.75 mg/kg/d)upon relapse to first remission in patients with SDNS/FRNS(odds ratio=3.14;95%confidence interval,0.97-9.87;P=0.034).rs117014418 in APOL4 was significantly associated with a decrease in eGFR of greater than 20%compared with the baseline in SDNS/FRNS patients(P=0.0001).Protein-protein intersection network construction suggested that HLA-DQA1 and HLA-DQB1 function together through GSDMA.Thus,SSNSWR belongs to non-HLA region-dependent nephropathy,and the HLA-DQA/DQB region is likely strongly associated with dis-ease relapse,especially in SDNS/FRNS.The study provides a novel approach for the GWAS strategy of SsNS and contributes to our understanding of the pathological mechanisms of SSNSWRandSDNS/FRNS.

关键词： Frequentrelapse Genome-wide association study Human leukocyte antigen region Steroid-sensitive nephrotic syndrome

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儿童阑尾周围脓肿保守治疗后管理方式的选择

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中华小儿外科杂志 2024年第2期45卷 175-179页

作者：张松贾慧敏中国医科大学附属盛京医院小儿外科沈阳110004

儿童阑尾炎并发阑尾周围脓肿的治疗方式一直存在争议。在急性阑尾炎并发阑尾周围脓肿的病例中,当患儿出现弥漫性腹膜炎和全身中毒症状时首选急诊手术治疗。除此之外,保守治疗广泛应用于并发阑尾周围脓肿患儿的早期治疗中。大量研究证实... 详细信息

儿童阑尾炎并发阑尾周围脓肿的治疗方式一直存在争议。在急性阑尾炎并发阑尾周围脓肿的病例中,当患儿出现弥漫性腹膜炎和全身中毒症状时首选急诊手术治疗。除此之外,保守治疗广泛应用于并发阑尾周围脓肿患儿的早期治疗中。大量研究证实了保守治疗的安全性和有效性,成功率高达80%~97%,并且保守治疗成功后,复发概率较低,约2%~34%。本研究对儿童急性阑尾炎并发阑尾周围脓肿的保守治疗及好转后管理方式的选择进行综述,建议保守治疗好转后的管理方式可优先选择积极随访观察,不必常规进行择期阑尾切除术,存在复发风险因素的患儿需提高警惕或建议择期阑尾切除术。

关键词：阑尾炎阑尾切除术阑尾周围脓肿保守治疗儿童

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