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Proteomic and Functional Analyses of Outer Membrane Vesicles Secreted by Vibrio splendidus

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中国海洋大学学报:英文版 2023年第5期22卷 1361-1369页

作者： SONg huimin RUAN Yilong LI ya ya.g Huirong ZHANg Weiwei School of Marine Sciences Ningbo UniversityNingbo 315832China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Ningbo UniversityNingbo 315211China

Vibrio splendidus is an important opportunistic pathogen ubiquitously present in the marine environment,exhibiting virulence to a variety of cultured *** extracellular products secreted by *** are crucial to bacterial survival and *** this study,the secretion of outer membrane vesicles(OMVs)by *** was determined,purified,and morphologically *** protein composition of OMVs was analyzed by proteomic *** results showed that approximately 120 proteins were contained in these OMVs,including outer membrane proteins,flagellins,ABC transporters,protease,and iron regulation proteins,etc.,which were involved in bacterial motility,formation of biofilms and the cell membrane components,and cellular localization based on their structural molecule activity,passive transmembrane transporter activity,channel activity,neurotransmitter receptor activity,extracellular ligand-gated ion channel activity,glutamate receptor activity,ligand-gated ion channel activity,and transmembrane signaling receptor *** explore the biological functions of OMVs in ***,the effects of OMVs on the bacterial adaption to iron limitation,antibiotic,and the coelomic fluid of the Apostichopus japonicus were *** study is the first time to show that *** secretes OMVs,and OMVs carry functional proteins that enhance bacterial survival under various stresses.

关键词： Vibrio splendidus outer membrane vesicles(OMVs) proteomic analysis biological function

Standardized ileal digestibility of amino acids in soybean meal fed to non-pregnant and pregnant sows

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Journal of Animal Science and Biotechnology 2024年第1期15卷 362-373页

作者： Ke Wang ya Wang Lei guo Yong Zhuo Lun Hua Lianqiang Che Shengyu Xu Ruinan Zhang Jian Li Bin Feng Zhengfeng Fang Xuemei Jiang yan Lin De Wu Institute of Animal Nutrition Key Laboratory for Animal Disease‑Resistance Nutrition of ChinaMinistry of EducationSichuan Agricultural University211 Huimin RoadWenjiang DistrictChengdu 611130SichuanChina

Background Two studies were designed to determine standard ileal crude protein(CP)and amino acid(AA)digestibility of soybean meal(SBM)from different origins fed to non-pregnant and pregnant *** solvent-extracted SBMs from soybeans produced in the USA,Brazil,and China were *** Exp.1,eight different diets were created:a nitrogen(N)-free diet and 7 experimental diets containing SBM from different origins as the only N *** non-pregnant,multiparous sows were arranged in an 8×8 Latin square design(8 periods and 8 diets).In Exp.2,the diet formula was the same as in *** gestating sows(parity 3)were assigned to 4 different diets in a replicated 4×3 Youden square design(three periods and four diets)in mid-gestation and again in late-gestation *** When fed to non-pregnant and late-gestating sows,the standardized ileal digestibility(SID)of CP and most AAs from different SBM were not significantly different(P>0.05).When fed to mid-gestating sows,the SID values for Arg,His,Lys,Phe,Cys,gly,Ser,and Tyr in SBM 1 were lower than in SBM 4 and 5(Pgher than in SBM 1 and 4(Pgnificantly greater SID values for Lys,Ala,and Asp during mid-gestation when compared with late-gestation stages(Pgestating sows had greater SID value for Val and lower SID value for Tyr when compared with non-pregnant and late-gestating sows(Pgnant sows had significantly greater SID value for Met when compared with gestating sows(Pgestating sows,the SID values for most AAs varied among SBM *** SID values for Lys,Met,Val,Ala,Asp,and Tyr in SBM were affected by sow gestation *** findings provide a cornerstone for accurate SBM use in sow diets.

关键词： Amino acids Sows Soybean meal Standardized ileal digestibility

小麦3种锈菌PCWDEs家族基因鉴定及表达分析

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中国农业大学学报 2024年第4期29卷 113-127页

作者：吴珂妍千慧敏赵俊溢李闯路亚南邢国珍刘娜郑文明河南农业大学生命科学学院郑州450002 省部共建小麦玉米作物学国家重点实验室郑州450002

为挖掘小麦锈菌侵染过程中发挥关键作用的植物细胞壁降解酶(Plant cell wall-degrading enzymes,PCWDEs)基因,通过生物信息学方法对小麦3种锈菌(叶锈菌、条锈菌和秆锈菌)基因组中的碳水化合物活性酶(Carbohydrate-active enzymes, CAZym... 详细信息

为挖掘小麦锈菌侵染过程中发挥关键作用的植物细胞壁降解酶(Plant cell wall-degrading enzymes,PCWDEs)基因,通过生物信息学方法对小麦3种锈菌(叶锈菌、条锈菌和秆锈菌)基因组中的碳水化合物活性酶(Carbohydrate-active enzymes, CAZymes)进行预测分析,在此基础上对其中的PCWDEs基因家族进行全基因组范围的筛选鉴定,并利用qRT-PCR方法对叶锈菌PCWDEs家族成员在侵染过程中的转录水平进行了检测分析。结果表明:1)小麦叶锈菌、条锈菌和秆锈菌基因组分别编码355、322和345个CAZymes家族成员基因以及160、153和158个PCWDEs基因;小麦3种锈菌共含75个保守CAZymes基因亚家族和94个PCWDEs直系同源基因簇。2)16个叶锈菌特异性PCWDEs基因在亲和(Pt15-‘中国春’)/非亲和(Pt15-‘云麦34’)互作过程中均受到不同程度的诱导表达,但非亲和互作中在侵染更早期就已受到诱导上调表达。3)叶锈菌特异性PCWDEs基因簇中有4个PCWDEs被鉴定为类扩展蛋白,其在侵染过程中受到诱导表达,说明类扩展蛋白可能在降解纤维素突破侵染屏障中发挥重要作用。综上,在小麦3种锈菌全基因组范围鉴定了PCWDEs基因,初步明确部分基因在锈菌侵染过程中的表达模式,PCWDEs可能在小麦锈菌致病过程中发挥重要作用。

关键词：小麦锈菌 PCWDEs 生物信息学分析 qRT-PCR 侵染机制

Boosting the piezoelectric property of relaxor ferroelectric single crystal via active manipulation of defect dipole polarization

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Journal of Materiomics 2023年第1期9卷 166-173页

作者： Qingyuan Hu huimin Liao Xin Liu Li Jin Kexin Song Yongyong Zhuang Zhuo Xu Vladimir ya Shur Xiaoyong Wei Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education&International Center for Dielectric ResearchSchool of Electronic ImformationXi'an Jiaotong UniversityXi'an710049China School of Natural Sciences and Mathematics Ural Federal UniversityEkaterinburg620000Russia

To further enhance the property of piezoelectric materials is of great significance to improve the overall performance of electro-mechanical *** in this work,we propose a thermal annealing and high temperature poling approach to achieve significantly enhanced piezoelectricity in Pb(In_(1/2)Nb_(1/2))O_(3)single bondPb(Mg_(1/3)Nb_(2/3))O_(3)single bondPbTiO_(3)(PIN-PMN-PT)crystals with a morphotropic phase boundary(MPB)*** main idea of our approach is to realize a more sufficiently polarized crystal via active manipulation of defects and orientation of defect *** of defect dipoles by the high temperature poling is proved by the piezo-response force ***,a d_(33)of 3300 pC/N and a SE of 0.25%are obtained,nearly 60%higher than that of conventionally poled ***,such a boosting of piezoelectric property is obtained under a maintained Curie *** research not only reveals the active control of defect dipole via modified poling method in the PIN-PMN-PT crystal,but also provides a feasible strategy to further improve the property of piezoelectric materials.

关键词： Piezoelectric property Relaxor ferroelectric single crystals Defects Defect polarization

基于Hedgehog通路探讨理肺消积丸对NSCLC A549细胞周期、增殖和迁移的影响

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世界科学技术-中医药现代化 2024年第2期26卷 320-327页

作者：刘素晓张丛丛孔德琪黄慧敏毛静王元元刘学芳冯素香李亚河南中医药大学第一附属医院郑州450000 河南中医药大学第一临床医学院郑州450046 河南中医药大学呼吸疾病中医药防治省部共建协同创新中心河南省中医药防治呼吸病重点实验室郑州450046

目的探讨理肺消积丸对A549细胞的影响作用及对Hedgehog通路的调控作用。方法采用MTT实验筛选理肺消积丸含药血清对A549细胞最佳干预浓度后,将细胞分为6组:对照组、理肺消积含药血清组、Hedgehog通路抑制剂组(Vismodegib组)、顺铂组、理... 详细信息

目的探讨理肺消积丸对A549细胞的影响作用及对Hedgehog通路的调控作用。方法采用MTT实验筛选理肺消积丸含药血清对A549细胞最佳干预浓度后,将细胞分为6组:对照组、理肺消积含药血清组、Hedgehog通路抑制剂组(Vismodegib组)、顺铂组、理肺消积含药血清+Vismodegib组、理肺消积含药血清+顺铂组。不同干预后,分别采用CCK-8实验检测细胞增殖能力,采用克隆形成实验观察细胞克隆形成能力,采用Transwell实验观察A549细胞迁移能力,采用流式细胞术观察细胞周期比例的变化,采用qRT-PCR和Western blot技术检测Hedgehog通路中gli1、Ptch1、Smo和CyclinD1 mRNA和蛋白表达的变化。结果浓度为10%的理肺消积丸含药血清能显著抑制A549细胞增殖(Pg1期到S期转变,同时抑制gli1、Ptch1、Smo和CyclinD1 mRNA和蛋白表达(Pgib有良好的协同作用。结论理肺消积丸可显著抑制A549增殖和迁移能力,作用机制可能与其调控Hedgehog通路阻滞细胞周期有关。

关键词：非小细胞肺癌理肺消积丸 A549细胞 Hedgehog通路细胞周期

基于自我决定理论的婴儿母乳回应性喂养干预方案的构建及应用

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护理研究 2024年第14期38卷 2476-2483页

作者：赵淑良邵清春祖新霞苗逸群刘文文姜汇敏李亚男王爱华张媛媛山东第二医科大学护理学院山东261053 山东第二医科大学附属医院

目的:构建基于自我决定理论的婴儿母乳回应性喂养干预方案并初步探讨其应用效果。方法:采用便利抽样的方法,选取2023年2月1日—5月31日在某三级甲等医院产科住院分娩的50例产妇作为研究对象,将其分为干预组和对照组,每组25例。基于自我... 详细信息

目的:构建基于自我决定理论的婴儿母乳回应性喂养干预方案并初步探讨其应用效果。方法:采用便利抽样的方法,选取2023年2月1日—5月31日在某三级甲等医院产科住院分娩的50例产妇作为研究对象,将其分为干预组和对照组,每组25例。基于自我决定理论,通过文献回顾、小组讨论、德尔菲专家函询构建婴儿母乳回应性喂养干预方案。对照组接受医院产科实施的常规护理措施,干预组在对照组基础上实施婴儿母乳回应性喂养干预方案,比较两组产妇回应性喂养知识水平、母乳喂养自我效能、母乳喂养动机、纯母乳喂养率、婴儿体重与身长。结果:婴儿母乳回应性喂养干预方案构建过程中,2轮专家函询问卷回收率均为100%,专家权威系数为0.92和0.94,变异系数为0.00~0.29和0.05~0.20,肯德尔和谐系数为0.214和0.165(均P<0.001)。干预后,干预组回应性喂养知识得分为(16.80±0.50)分,母乳喂养自我效能得分为(58.16±9.33)分,自主动机得分为(61.00±5.79)分,受控动机得分为(29.08±3.27)分,均高于对照组[回应性喂养知识得分为(13.56±2.35)分,母乳喂养自我效能得分为(44.84±8.60)分,自主动机得分为(51.04±8.36)分,受控动机得分为(24.00±3.81)分];干预组出院当天、出院1周、产后第42天的纯母乳喂养率均高于对照组。结论:本研究构建的婴儿母乳回应性喂养干预方案具有一定的科学性与可靠性,有利于提高产妇回应性喂养知识、母乳喂养自我效能,帮助产妇树立母乳喂养动机,改善纯母乳喂养率。

关键词：自我决定理论母乳喂养回应性喂养德尔菲法护理

One-step synthesis and electromagnetic absorption properties of high entropy rare earth hexaborides(HE REB6)and high entropy rare earth hexaborides/borates(HE REB6/HE REB03)composite powders

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Journal of Advanced Ceramics 2021年第1期10卷 62-77页

作者： Weiming ZHANg Biao ZHAO huimin XIANg Fu-Zhi DAI Shijiang WU yanchun ZHOU Science and Technology on Advanced Functional Composite Laboratory Aerospace Research Institute of Materials&Processing TechnologyBeijing 100076China Henan Key Laboratory of Aeronautical Materials and Application Technology School of Material Science and EngineeringZhengzhou University of AeronauticsZhengzhou 450046China Zibo Firststar New Material Incorporated Co. Ltd.Zibo 255000China

Considering the emergence of severe electromagnetic interference problems,it is vital to develop electromagnetic(EM)wave absorbing materials with high dielectric,magnetic loss and optimized impedance ***,realizing the synergistic dielectric and magnetic losses in a single phase material is still a ***,high entropy(HE)rare earth hexaborides(REB6)powders with coupling of dielectric and magnetic losses were designed and successfully synthesized through a facial one-step boron carbide reduction method,and the effects of high entropy borates intermedia phases on the EM wave absorption properties were *** HE REB6 ceramics including(Ce0.2Y0.2Sm0.2Er0.2Yb0.2)B6,(Ce0.2Hu0.2Sm0.2Er0.2Yb0.2)B6,(Ce0.2Y0.2Eu0.2Er0.2Yb0.2)B6,(Ce0.2ya2Sm0.2Eu0.2Yb0.2)B6,and(Nd0.2Y0.2Sm0.2Eu0.2Yb0.2)B6 possess CsCl-type cubic crystal structure,and their theoretical densities range from 4.84 to 5.25 g/cm^(3).(Ce02Y0.2Sm0.2Er0.2Yb02)B6 powders with the average particle size of 1.86 jim were found to possess the best EM wave absorption properties among these *** RLmin value of(Ce0.2Y0.2Sm0.2Er0.2Yb0.2)B6 reaches-33.4 dB at 11.5 gHz at thickness of 2 mm;meanwhile,the optimized effective absorption bandwidth(EAB)is 3.9 gHz from 13.6 to 17.5 gHz with a thickness of 1.5 *** introduction of HE REB03(RE=Ce,Y,Sm,Eu,Er,Yb)as intermediate phase will give rise to the mismatching impedance,which will further lead to the reduction of reflection ***,the HEREB6/HEREB03 still possess wide effective absorption bandwidth of 4.1 gHz with the relative low thickness of 1.7 *** the better stability,low density,and good EM wave absorption properties,HE REB6 ceramics are promising as a new type of EM wave absorbing materials.

关键词： high entropy rare earth hexaborides(HE REB6) one-step synthesis electromagnetic wave absorbing properties synergistic dielectric and magnetic losses wide effective absorption bandwidth

Phenotypic and functional characteristic of a newly dentified CDS＋Foxp3-CDI03＋ regulatory T cells

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Journal of Molecular Cell Biology 2014年第1期8卷 81-92页

作者： ya Liu Qin Lan Ling Lu Maogen Chen Zanxian Xia Jilin Ma Julie Wang huimin Fan Yi Shen Bernhard Ryffel David Brand Francisco Quismorio Zhongmin Liu David A. Horwitz Anping Xu Song guo Zheng Department of Nephrology Sun Yat-sen Memorial Hospital of Sun Yat-sen University Guangzhou 510120 China Division of Rheumatology and Immunology Department of Medicine University of Southern California Keck School of Medicine Los Angeles CA 90033 USA Institute of Immunology Shanghai East Hospital at Tongji University Shanghai200120 China University of Orleans and CNRS UMR7355 3b rue de la Ferollerie Orleans 45071 France Veterans Affairs Medical Center Memphis TN 38104 USA

TgF-β and Foxp3 expressions are crucial for the induction and functional activity of CD4＋Foxp3＋ regulatory T （iTreg） cells. Here, we demonstrate that although TgF-β-primed CD8＋ cells display much lower Foxp3 expression, their suppressive capacity is equivalent to that of CD4＋ iTreg cells, and both Foxp3- and Foxp3＋ CD8＋ subsets have suppressive activities in vitro and in vivo. CD8＋Foxp3- iTreg cells produce little IFN-γ but almost no IL-2, and display a typical anergic phenotype. Among phenotypic markers expressed in CD8＋Foxp3- cells, we identify CD103 expression particularly crucial for the generation and function of this subset. Moreover, IL-IO and TgF-β signals rather than cytotoxicity mediate the suppressive effect of this novel Treg population. Therefore, TgF-β can induce both CD8＋Foxp3- and CD8＋Foxp3＋ iTreg subsets, which may represent the unique immunoregulatory means to treat autoimmune and inflammatory diseases.

关键词： CD8+ regulatory T cells TgF-β Foxp3 CD103

A Review of Intercultural Communication between Xiang and ya

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China & The World Cultural Exchange 2007年第4期73卷 30-31页

作者： Huang huimin & Wang Xiaojing

yale University's longstanding association with China's central south province of Hunan takes us back more than a hundred years. Ever since the beginning of the 20th century, long-term cooperation programs based on th... 详细信息

关键词：美国耶鲁大学雅礼协会湖南文化交流教育国际合作湘雅交流湘雅医学院历史综述