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Engineering trienzyme cascade-triggered fluorescent immunosensor platform by sequentially integrating alkaline phosphatase, tyrosinase and horseradish peroxidase

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Chinese Chemical Letters 2023年第4期34卷 444-448页

作者： Yujie Sun Lei Wen huili Ma Wenlin Ma Zhenqian fu Yinhui Li Chengwu Zhang Lin Li Jinhua Liu Institute of Advanced Materials(IAM) Key Laboratory of Flexible Electronics(KLOFE)Nanjing Tech UniversityNanjing 211816China Key Laboratory for Green Organic Synthesis and Application of Hunan Province Key Laboratory of Environmentally Friendly ChemistryApplication of Ministry of EducationCollege of ChemistryXiangtan UniversityXiangtan 411105China School of Basic Medical Sciences Shanxi Medical UniversityTaiyuan 310003China

Mulit-enzyme cascades are a major type of chemical transformations and play a crucial role in biological signal transduction and metabolism. Herein, a trienzyme cascade-triggered fluorescent immunosensor platform was constructed by sequentially integrating alkaline phosphatase(ALP), tyrosinase(TYR)and horseradish peroxidase(HRP). The proposed platform was based on HRP-induced a rapid in situ fluorogenic reaction between dopamine(DA) and 1,5-dihydroxynaphthalene(DHA) to produce a strong yellow azamonardine fluorescent compound(AFC). The obtained AFC was clearly characterized by highresolution mass spectrum,1H NMR,^(13)C NMR and theoretical calculations. The integration of the twoenzyme system(TYR and HRP) or three-enzyme system(ALP, TYR and HRP) led to a maximum of 400.0-fold and 250.0-fold fluorescence enhancements, respectively. Using cardiac troponin I(c Tn I) as the model antigen, a trienzyme cascade-triggered fluorescent immunosensor platform was developed for quantitative detecting c Tn I in a wide linear range from 2 ng/m L to 150 ng/m L with a detection limit of 0.67ng/m L. In addition, the proposed platform was successfully applied in detection of c Tn I in serum of clinical patients. Overall, the developed fluorescent immunosensor performs powerful implications for researching enzyme cascade systems in the field of biomedicine.

关键词： Enzyme cascade Alkaline phosphatase Tyrosinase Horseradish peroxidase Cardiac troponin I

Core-shell lipid-polymer nanoparticles as a promising ocular drug delivery system to treat glaucoma

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Chinese Chemical Letters 2020年第2期31卷 494-500页

作者： Yang Zhou Aiping Fang Fazhan Wang huili Li Quansheng Jin Lingjing Huang Chunmei fu Jun Zeng Zhaohui Jin Xiangrong Song State Key Laboratory of Biotherapy and Cancer Center/Department of Pharmacy West China Hospitaland Collaborative Innovation Center for BiotherapySichuan UniversityChengdu 610041China West China School of Pharmacy Sichuan UniversityChengdu 610041China

Nowadays,tremendous researches have been focused on the core-shell lipid-polymer nanoparticles(LPNs) due to the advantages of both liposomes and polymer *** this work,LPNs were applied to encapsulate brinzolamide(Brz-LPNs) for achieving sustained drug release,improving drug corneal permeation and enhancing drug topical therapeutic *** structure of Brz-LPNs was composed of poly(lactic-co-glycolic) acid(PLGA) nanocore which encapsulated Brz(Brz-NPs) and lipid shell around the ***-LPNs were prepared by a modified thin-film dispersion *** the parameters optimization of Brz-LPNs,optimal Brz-LPNs showed an average particle size of151.23±1.64 nm with a high encapsulation efficiency(EE) of 86.7%±2.28%.The core-shell structure of Brz-LPNs were confirmed by transmission electronic microscopy(TEM).Fourier transformed infrared spectra(FTIR) analysis proved that Brz was successfully entrapped into ***-LPNs exhibited obvious sustained release of Brz,compared with AZOPT^■ and ***,the corneal accumulative permeability of Brz-LPNs significantly increased compared to the commercial available formulation(AZOPT^■) in ***,Brz-LPNs(1 mg/mL Brz) showed a more sustained and effective intraocular pressure(IOP) reduction than Brz-LPs(1 mg/mL) and AZOPT^■(10 mg/mL Brz) in *** conclusion,Brz-LPNs,as promising ocular drug delivery systems,are well worth developing in the future for glaucoma treatment.

关键词： Core-shell lipid-polymer nanoparticles Ocular drug delivery system Sustained release Corneal permeation Intraocular pressure

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汽车端到端自动驾驶现状与发展趋势

时代汽车 2024年第13期 4-6,109页

作者：胡爽禄华贤平窦民费慧莉伏子敬同济大学经济与管理学院上海200092 无锡车城智联科技有限公司江苏无锡214000 上海人工智能实验室上海200032 浙江大学管理学院浙江杭州310000

端到端自动驾驶研究的目的是通过简化系统架构、提高系统性能、降低开发成本、提高泛化能力和推动技术发展,实现更加先进、可靠的自动驾驶技术。端到端自动驾驶是指直接从原始传感器数据输入到车辆控制输出的端到端学习方法,旨在简化自... 详细信息

端到端自动驾驶研究的目的是通过简化系统架构、提高系统性能、降低开发成本、提高泛化能力和推动技术发展,实现更加先进、可靠的自动驾驶技术。端到端自动驾驶是指直接从原始传感器数据输入到车辆控制输出的端到端学习方法,旨在简化自动驾驶系统的复杂性并提高系统性能。端到端自动驾驶技术的意义不仅在于技术层面的突破和创新,更在于其对交通、经济和社会的深远影响。目前端到端自动驾驶是行业的共识,随着技术的不断进步和应用的推广,端到端自动驾驶技术将为出行方式带来革命性的变化,推动智能交通和智慧出行的发展。

关键词：端到端自动驾驶智能交通智慧出行

The chimeric gene atp6c confers cytoplasmic male sterility in maize by impairing the assembly of the mitochondrial ATP synthase complex

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Molecular Plant 2022年第5期15卷 872-886页

作者： huili Yang Yadong Xue Bing Li Yanan Lin Haochuan Li Zhanyong Guo Weihua Li Zhiyuan fu Dong Ding Jihua Tang National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhouChina The Shennong Laboratory ZhengzhouChina

Cytoplasmic male sterility(CMS)is a powerful tool for the exploitation of hybrid heterosis and the study of signaling and interactions between the nucleus and the cytoplasm.C-type CMS(CMS-C)in maize has long been used in hybrid seed production,but the underlying sterility factor and its mechanism of action remain *** this study,we demonstrate that the mitochondrial gene atp6c confers male sterility in CMS-C *** ATP6C protein shows stronger interactions with ATP8 and ATP9 than ATP6 during the assembly of F1F0-ATP synthase(F-type ATP synthase,ATPase),thereby reducing the quantity and activity of assem-bled F_(1)F_(o)-ATP *** contrast,the quantity and activity of the F1'component are increased in CMS-C *** F1F0-ATP synthase activity causes accumulation of excess protons in the inner membrane space of the mitochondria,triggering a burst of reactive oxygen species(ROS),premature programmed cell death of the tapetai cells,and pollen ***,our study identifies a chimeric mitochondrial gene(ATP6C)that causes CMS in maize and documents the contribution of ATP6C to F1F0-ATP synthase assembly,thereby providing novel insights into the molecular mechanisms of male sterility in plants.

关键词： maize cytoplasmic male sterility atp6c ATP synthase mitochondria

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基于3S技术构建森林景观信息系统

首都师范大学学报（自然科学版） 2003年第2期24卷 102-108页

作者：张志锋谢志茹宫辉力付瑞海首都师范大学资源环境与地理信息系统北京市重点实验室北京100037 北京市林业局野生动物保护自然保护区管理站北京100035

介绍了地理信息系统技术 (GIS)、遥感技术 (RS)和全球定位系统技术 (GPS)在森林景观研究中的应用和整个 3S系统的集成模式 .在 3S系统中遥感用于获取森林景观资源的有关信息 ;全球定位系统用于定位采集地面数据和导航 ;而地理信息系统... 详细信息

介绍了地理信息系统技术 (GIS)、遥感技术 (RS)和全球定位系统技术 (GPS)在森林景观研究中的应用和整个 3S系统的集成模式 .在 3S系统中遥感用于获取森林景观资源的有关信息 ;全球定位系统用于定位采集地面数据和导航 ;而地理信息系统用于管理和处理收集到的有关森林景观资源的各种信息 .

关键词：森林景观信息系统地理信息系统遥感全球定位系统 3S技术 GIS RS GPS 系统集成森林资源

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围产期护理缺失量表的汉化及信效度检验

International Journal of Nursing Sciences 2024年第1期11卷 106-112页

作者：丁胜兰王鑫王清霞沈涓谢惠莉付秀娟廖露茜陈娇娇朱莲黄静杨思源黄秀华张轶岚 Birth Room Chengdu Women’s and Children’s Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina Department of Nursing Chengdu Women’s and Children’s Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina Department of Medical Imaging The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina School of Health in Social Science University of EdinburghEdinburghUnited Kingdom School of Nursing Peking UniversityBeijingChina Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqingChina Surgery Intensive Care Unit Chengdu Women’s and Children’s Central HospitalSchool of MedicineUniversity of Electronic Science and Technology of ChinaChengduChina

目的翻译围产期护理缺失量表,并对其进行信度和效度检验,为国内开展围产期护理缺失的量性研究提供可靠的测评工具。方法采用美国矫形外科医师学会循证医学委员会推荐的跨文化调试量表指南对英文版围产期护理缺失量表进行翻译、回译-文... 详细信息

目的翻译围产期护理缺失量表,并对其进行信度和效度检验,为国内开展围产期护理缺失的量性研究提供可靠的测评工具。方法采用美国矫形外科医师学会循证医学委员会推荐的跨文化调试量表指南对英文版围产期护理缺失量表进行翻译、回译-文化调适及内容验证,形成中文版围产期护理缺失量表,并通过横断面研究验证其信效度。于2023年2月至4月采用便利抽样法,选取西南地区14所不同级别医院491名助产士进行问卷调查。采用项目分析检验中文版量表条目的鉴别度,采用探索性和验证性因子分析评价量表的结构效度,采用内容效度指数和Cronbach'sa系数评价内容效度和信度。结果围产期护理缺失.量表包括A、B两部分。中文版围产期护理缺失量表A部分的项目-总量表相关系数为0.641 -0.866, B部分为0.644~0.819(P<0.01)。A部分和B部分的量表水平内容效度指数均为0.95,条目水平内容效度指数为0.86~1.00。探索性因子分析分别提取了A部分(必要性护理、基本护理和产后护理)和B部分(沟通、人力资源和物质资源)各3个因子,占总方差的70.186%和71.984%。量表A、B部分的Cronbach's a系数分别为0.968、0.940。结论中文版围产期护理缺失量表具有良好的信度和效度,可作为国内评价围产期护理缺失情况和相关原因的工具。未来需要进行更大样本量的研究,以验证该工具在中国本土的适用性。

关键词：助产士护理缺失围产期护理信度调查及问卷效度

Technology Development Analysis on Low Carbon for Power of Heavy-Duty Commercial Vehicle

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Automotive Innovation 2019年第1期2卷 64-70页

作者： Jiangwei Liu Jun Li Yongjun Wang Dalu Dong Yansheng Yin Huayu Jin Shijie Sun huili Dou Yanbo Shi Lei fu Fei Ge China FAW Group Corporation Limited Changchun 130000China Tsinghua University Beijing 100084China

Commercial vehicle industry worldwide is facing challenges from environmental pressures,stringent limits of CO_(2) emission,governmental regulations as well as ever-increased customer *** paper analyzes the above-mentioned challenges,especially in China,including the potential improvement to increase the brake thermal efficiency(BTE),with five levels of BTE proposed,ranging from current 45 to 60%in future,corresponding to China fuel consumption regulation(CFCR)in different *** authors also proposed the technology roadmaps to meet the upcoming CFCR3 and CFCR4;finally,the authors draw the conclusions to conform with ever-stringent regulation in China.

关键词： Heavy-duty diesel engine(HDDE) Low carbon fuel consumption

四川里伍铜尾矿中铁闪锌矿和磁黄铁矿的高效分离回收试验

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金属矿山 2024年第6期 123-129页

作者：傅开彬李贺付华洪刘泽铭孔云龙朱乐明西南科技大学环境与资源学院四川绵阳621010 四川里伍铜业股份有限公司西科大里伍铜业产业技术创新研究院四川甘孜626000 西南科大四川天府新区创新研究院四川成都610299 会理鹏晨废渣利用有限公司四川会理615100

为综合回收四川里伍铜矿选铜尾矿中的有价元素锌、硫,采用优先浮选试验筛选了铁闪锌矿和磁黄铁矿的高效活化剂、捕收剂等,并进行了闭路试验。结果表明:试验样采用1粗4精2扫选锌、1粗3精3扫选硫闭路工艺流程处理,可获得锌品位45.62%、锌... 详细信息

为综合回收四川里伍铜矿选铜尾矿中的有价元素锌、硫,采用优先浮选试验筛选了铁闪锌矿和磁黄铁矿的高效活化剂、捕收剂等,并进行了闭路试验。结果表明:试验样采用1粗4精2扫选锌、1粗3精3扫选硫闭路工艺流程处理,可获得锌品位45.62%、锌回收率85.83%的锌精矿,硫品位39.98%、硫回收率75.67%的硫精矿,锌精矿达到七级产品质量要求,硫精矿达到二级产品质量要求,较好地实现了铁闪锌矿和磁黄铁矿的浮选分离。

关键词：铜尾矿铁闪锌矿磁黄铁矿高效分离

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动态车辆路径问题的隐分区灵活分批策略

同济大学学报（自然科学版） 2013年第5期41卷 676-679,686页

作者：熊浩符卓鄢慧丽中南大学交通运输工程学院湖南长沙410083 长沙理工大学交通运输工程学院湖南长沙410004

在一般分区分批旅行商策略的基础上,提出了隐分区灵活分批旅行商策略,并进行了有效性分析.新策略主要从2个方面做了改进:①通过设置虚拟分区,保持了分区顾客的到达率不变,使顾客群的形成时间减少;②对决策时间进行了调整,允许在顾客群... 详细信息

在一般分区分批旅行商策略的基础上,提出了隐分区灵活分批旅行商策略,并进行了有效性分析.新策略主要从2个方面做了改进:①通过设置虚拟分区,保持了分区顾客的到达率不变,使顾客群的形成时间减少;②对决策时间进行了调整,允许在顾客群形成时间与决策时间之内出现的新顾客进入决策,使进入计划的顾客更多,从而使路径平均距离更短.最后,实例仿真验证了策略的优越性.

关键词：车辆路径问题实时优化隐分区灵活分批策略动态需求