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The Statistical Power of Inclusive Composite Interval Mapping in Detecting Digenic Epistasis Showing Common F_2 Segregation Ratios

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Journal of Integrative Plant Biology 2012年第4期54卷 270-279页

作者： Luyan Zhang huihui li Jiankang Wang Institute of Crop Sciences The National Key Facility for Crop Gene Resources and Genetic Improvementand CIMMYT ChinaChinese Academy of Agricultural SciencesBeijing 100081China

Epistasis is a commonly observed genetic phenomenon and an important source of variation of complex traits, which could maintain additive variance and therefore assure the long-term genetic gain in breeding. Inclusive composite interval mapping （ICIM） is able to identify epistatic quantitative trait loci （QTLs） no matter whether the two interacting QTLs have any additive effects. In this article, we conducted a simulation study to evaluate detection power and false discovery rate （FDR） of ICIM epistatic mapping, by considering F2 and doubled haploid （DH） populations, different F2 segregation ratios and population sizes. Results indicated that estimations of QTL locations and effects were unbiased, and the detection power of epistatic mapping was largely affected by population size, heritability of epistasis, and the amount and distribution of genetic effects. When the same likelihood of odd （LOD） threshold was used, detection power of QTL was higher in F2 population than power in DH population; meanwhile FDR in F2 was also higher than that in DH. The increase of marker density from 10 cM to 5 cM led to similar detection power but higher FDR. In simulated populations, ICIM achieved better mapping results than multiple interval mapping （MIM） in estimation of QTL positions and effect. At the end, we gave epistatic mapping results of ICIM in one actual population in rice （Oryza sativa L.）.

关键词： Epistasis false discovery rate inclusive composite interval mapping power analysis simulation study.

企业数字化转型:概念界定、主要数字技术与衡量方法

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金融经济 2024年第2期 60-69,100页

作者：钟晓龙李慧慧中国光大集团博士后科研工作站中国人民大学财政金融学院博士后科研流动站南京师范大学商学院

近年来,随着数字技术的进步和经济社会的变革,数字化转型成为国内外学者重点关注的研究领域之一。本文在区分与企业数字化转型相关的术语“数据化”和“数字化”的基础上,凝炼了企业数字化转型的关键要素和概念,具体包含数字技术、转型... 详细信息

近年来,随着数字技术的进步和经济社会的变革,数字化转型成为国内外学者重点关注的研究领域之一。本文在区分与企业数字化转型相关的术语“数据化”和“数字化”的基础上,凝炼了企业数字化转型的关键要素和概念,具体包含数字技术、转型范围、转型结果三个要素。进一步地,对构成企业数字化转型基础的主要数字技术,包括人工智能、区块链、云计算、大数据等进行了阐述,探讨了这些数字技术的差异化特征和优势。同时,按照产业数字化转型发展阶段对企业数字化转型衡量方法进行了分类讨论,发现年报文本分析法是目前衡量企业数字化转型程度较为合理的一种方法。本文的研究结果对于推动企业数字化转型具有重要的理论和实践意义。

关键词：数字技术数字经济数字化转型衡量方法文本分析

老年患者膝关节置换术中血糖变异指数与术后谵妄的相关性

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临床麻醉学杂志 2024年第10期40卷 1058-1062页

作者：潘鑫蔡宁李慧慧官双双汪辰宇安徽省阜阳市人民医院麻醉科 236000

目的探讨老年患者膝关节置换术中血糖变异指数(GLUCV)与术后谵妄(POD)的相关性。方法回顾性分析择期行膝关节置换术的老年患者112例,男16例,女96例,年龄65~80岁,BMI 22.0~28.0 kg/m^(2),ASAⅠ或Ⅱ级。根据术后5 d内是否发生POD将患者分... 详细信息

目的探讨老年患者膝关节置换术中血糖变异指数(GLUCV)与术后谵妄(POD)的相关性。方法回顾性分析择期行膝关节置换术的老年患者112例,男16例,女96例,年龄65~80岁,BMI 22.0~28.0 kg/m^(2),ASAⅠ或Ⅱ级。根据术后5 d内是否发生POD将患者分为两组:POD组和非POD组。收集术前最后1次简易智力状态检查量表(MMSE)得分、手术时间、术中局部脑氧饱和度(rScO_(2))值降低幅度超过rScO_(2)基础值13%(ΔrScO_(2)>13%)的时间,术前血红蛋白(Hb)、C反应蛋白(CRP)浓度,入室前、麻醉诱导前、麻醉诱导后5 min、切皮后1 min、离室前采集指尖血糖浓度等资料,计算GLUCV。采用单因素和多因素Logistic回归分析老年膝关节置换术患者POD的危险因素,并计算比值比(OR)和95%可信区间(CI)。绘制受试者工作特征(ROC)曲线,通过计算曲线下面积(AUC)分析GLUCV的预测价值。结果术后5 d内发生POD 30例(26.8%)。与非POD组比较,POD组手术时间和术中ΔrScO_(2)>13%时间明显延长(P13%时间延长(OR=3.542,95%CI 1.380~9.093,P=0.011)、CRP浓度升高(OR=1.984,95%CI 1.078~3.650,P=0.015)、GLUCV升高(OR=5.257,95%CI 1.690~16.353,P=0.008)是老年膝关节置换术患者发生POD的危险因素。GLUCV预测老年膝关节置换术患者POD的AUC为0.747(95%CI 0.650~0.844,P<0.001),临界值为23.4%,敏感性为71.4%,特异性为63.3%。结论老年患者膝关节置换术GLUCV与POD存在明显相关性,同时GLUCV对于老年患者膝关节置换术发生POD具有良好的预测效能。

关键词：膝关节置换术术后谵妄血糖变异指数危险因素血糖

Ordering of high-density markers by the k-Optimal algorithm for the traveling-salesman problem

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The Crop Journal 2020年第5期8卷 701-712页

作者： Luyan Zhang huihui li Lei Meng Jiankang Wang National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Construction of accurate and high-density linkage maps is a key research area of *** investigated the efficiency of genetic map construction(MAP)using modifications of the k-Optimal(k-Opt)algorithm for solving the traveling-salesman problem(TSP).For TSP,different initial routes resulted in different optimal *** most optimal solution could be found only by use of as many initial routes as *** for MAP,a large number of initial routes resulted in one optimal order.k-Opt using open route length gave a slightly higher proportion of correct orders than the method of adding one virtual marker and using closed route *** frequency(REC)and logarithm of odds(LOD)score gave similar proportions of correct order,higher than that given by genetic *** missing markers and genotyping error reduced ordering accuracy,but the best order was still achieved with high probability by comparison of the optimal orders from multiple initial *** time increased rapidly with marker number,and 2-Opt took much less time than *** 2-Opt algorithm was compared with ordering methods used in two other software *** best method was 2-Opt using open route length as the criterion to identify the optimal order and using REC or LOD as the measure of distance between *** describe a unified software interface for using k-Opt in high-density linkage map construction for a wide range of genetic populations.

关键词： optimal algorithm traveling

The p53/miR-34 axis in development and disease

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Journal of Molecular Cell Biology 2014年第3期8卷 214-230页

作者： Matjaz Rokavec huihui li Longchang Jiang and Heiko Hermeking Experimental and Molecular Pathology Institute of Pathology Ludwig-Maximilians-Universitat MLinchen 80337 Munich Germany German Cancer Consortium (DKTK) 69120 Heidelberg Germany German Cancer Research Center (DKFZ) 69120 Heidelberg Germany

The tumor suppressor p53 is one of the most frequently mutated genes in human cancers. MicroRNAs （miRNAs） are small non-protein coding RNAs that regulate gene expression on the post-transcriptional level. Recently, it was shown that p53 regulates the expression of several miRNAs, thereby representing an important mechanism of p53 signaling. Several independent studies identified the members of the miR-34 family as the most prevalent p53-induced miRNAs, miR-34s are frequently silenced in variety of tumor entities, suggesting that they are important tumor suppressors. Indeed, ectopic expression of miR-34s inhibits proliferation, epithelial to mes- enchymat transition, migration, invasion, and metastasis of various cancer celt entities. Moreover, delivery or re-expression of miR-34 leads to notable repression of tumor growth and metastasis in cancer mouse models, and may therefore represent an efficient strategy for future cancer therapeutics. Besides their crucial functions in cancer, members of the miR-34 family also play important roles in spermatogenesis, stem cell differentiation, neuronal development, aging, and cardiovascular functions. Consequently, miR-34 has also been implicated in various non-cancerous diseases, such as brain disorders, osteoporosis, and cardiovascular complications.

关键词： p53 miR-34 miR-34a miR-34b/c cancer metastasis tumor suppression

Estimation of statistical power and false discovery rate of QTL mapping methods through computer simulation

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Chinese Science Bulletin 2012年第21期57卷 2701-2710页

作者： li huihui ZHANG LuYan WANG JianKang Institute of Crop Sciences The National Key Facility for Crop Gene Resources and Genetic Improvementand C1MMYT ChinaChinese Academyof Agricultural SciencesBeijing 100081China

Many QTL mapping methods have been developed in the past two ***,the best method should have a high detection power but a low false discovery rate (FDR).Power and FDR cannot be derived theoretically for most QTL mapping methods,but they can be properly evaluated using computer *** this paper,we used four genetic models (two for independent loci and two for linked loci) to illustrate power and FDR estimation for interval mapping (IM) and inclusive composite interval mapping (ICIM).For each model,we simulated 1000 populations each of 200 doubled haploids.A support interval (SI) was first defined to indicate to which predefined QTL the significant QTL *** was calculated by counting the number of simulation runs with significant peaks higher than the logarithm of odds (LOD) threshold in the *** trait loci not identified in any SIs were viewed as false *** FDR is the rate at which QTLs are identified as significant when they are actually *** results allowed us to estimate power and FDR of IM and ICIM for two independent and two linkage genetic *** estimates allowed us to readily compare the efficiencies of different statistical methods for QTL mapping,including the ability to separate linkage,under a wide range of genetic *** used IM and ICIM as examples of how to estimate power and FDR,but the principles shown in this paper can be used for power analysis and comparison of any other QTL mapping methods,especially those based on interval tests.

关键词： QTL定位计算机模拟定位方法统计方法估计假发遗传模型复合区间作图

QTL IciMapping:Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations

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The Crop Journal 2015年第3期3卷 269-283页

作者： Lei Meng huihui li Luyan Zhang Jiankang Wang The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science and CIMMYT China OfficeChinese Academy of Agricultural Sciences

QTL Ici Mapping is freely available public software capable of building high-density linkage maps and mapping quantitative trait loci(QTL) in biparental populations. Eight functionalities are integrated in this software package:(1) BIN: binning of redundant markers;(2) MAP: construction of linkage maps in biparental populations;(3) CMP: consensus map construction from multiple linkage maps sharing common markers;(4) SDL: mapping of segregation distortion loci;(5) BIP: mapping of additive, dominant, and digenic epistasis genes;(6) MET: QTL-by-environment interaction analysis;(7) CSL: mapping of additive and digenic epistasis genes with chromosome segment substitution lines; and(8) NAM: QTL mapping in NAM populations. Input files can be arranged in plain text, MS Excel 2003, or MS Excel 2007 formats. Output files have the same prefix name as the input but with different extensions. As examples, there are two output files in BIN, one for summarizing the identified bin groups and deleted markers in each bin, and the other for using the MAP functionality. Eight output files are generated by MAP, including summary of the completed linkage maps, Mendelian ratio test of individual markers, estimates of recombination frequencies, LOD scores, and genetic distances, and the input files for using the BIP, SDL,and MET functionalities. More than 30 output files are generated by BIP, including results at all scanning positions, identified QTL, permutation tests, and detection powers for up to six mapping methods. Three supplementary tools have also been developed to display completed genetic linkage maps, to estimate recombination frequency between two loci,and to perform analysis of variance for multi-environmental trials.

关键词： Biparental populations Map construction QTL mapping Software

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蛋白质聚集体的选择性自噬降解:聚集体自噬

中国细胞生物学学报 2024年第1期46卷 129-138页

作者：贾白慧李绘绘卢克锋四川大学华西医院生物治疗全国重点实验室成都610041

细胞通过蛋白质稳态的保持来支撑其蛋白质组的功能网络。由分子伴侣、泛素蛋白酶体和细胞自噬组成蛋白质稳态维持体系。稳态异常导致蛋白质聚集体的积累并引起衰老和各种疾病。细胞内选择性自噬途径清除蛋白质聚集体的机制被称为聚集体... 详细信息

细胞通过蛋白质稳态的保持来支撑其蛋白质组的功能网络。由分子伴侣、泛素蛋白酶体和细胞自噬组成蛋白质稳态维持体系。稳态异常导致蛋白质聚集体的积累并引起衰老和各种疾病。细胞内选择性自噬途径清除蛋白质聚集体的机制被称为聚集体自噬。该文介绍了聚集体自噬的上游即各种聚集体的形成、介导聚集体自噬的底物受体分子以及基于聚集体自噬的药物研发研究。

关键词：聚集体细胞自噬生物分子聚合物相变受体分子

不同碳纳米管掺量的聚合物复合改性沥青路用性能研究

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山东化工 2024年第15期53卷 77-79页

作者：李慧慧张恒王浩萌山东省路桥集团有限公司山东济南250014

对碳纳米管/SBS复合改性沥青制备工艺过程中的碳纳米管添加工艺与碳纳米管掺量进行研究,结果显示,溶液共混及机械共混的制备工艺有助于碳纳米管材料在SBS改性沥青中分散,同时,CNTs的掺量应控制在0.5%以内。路用性能研究表明,碳纳米管/... 详细信息

对碳纳米管/SBS复合改性沥青制备工艺过程中的碳纳米管添加工艺与碳纳米管掺量进行研究,结果显示,溶液共混及机械共混的制备工艺有助于碳纳米管材料在SBS改性沥青中分散,同时,CNTs的掺量应控制在0.5%以内。路用性能研究表明,碳纳米管/聚合物复合改性沥青相较于单独的CNTs改性沥青和SBS改性沥青,能发挥更好的改性作用。这种复合改性可以有效提升改性沥青的高温抗车辙性能和疲劳性能。本研究为碳纳米管在沥青路面工程中的应用提供了理论依据和实践指导。

关键词：碳纳米管改性工艺改性沥青

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