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Genome-wide profiling of circular RNAs,alternative splicing,and R-loops in stem-differentiating xylem of Populus trichocarpa

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Journal of Integrative Plant Biology 2021年第7期63卷 1294-1308页

作者： Xuqing Liu Yubang Gao Jiakai Liao Miao Miao Kai Chen Feihu Xi Wentao Wei huihui wang Yongsheng wang XiXu Anireddy S.N.Reddy Lianfeng Gu Basic Forestry and Proteomics Research Center College of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research Center College of Life ScienceFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColorado 80523USA

Circular RNAs(circRNAs)are a recently dis-covered type of non‐coding RNA derived from pre‐mRNAs.R‐loops consist of a DNA:RNA hybrid andthe associated single‐stranded ***-dopsis thaliana,circRNA:DNA R‐loops regulatealternative splicing(AS)ofSEPALLATA3(SEP3).However,the occurrence and functions ofcircRNAs and R‐loops inPopulus trichocarpaarelargely ***,we performed circRNA‐enriched sequencing in the stem‐differentiatingxylem(SDX)*** identified 2,742distinct circRNAs,including circ‐CESA4,circ‐IRX7,and circ‐GUX1,which are generated from genesinvolved in cellulose,and hemicellulose biosyn-thesis,*** investigate the roles ofcircRNAs in modulating alternative splicing(AS),we detected 7,836 AS events using PacBio Iso‐Seq and identified 634 circRNAs that overlappedwith 699 AS ***,using DNA:RNAhybrid immunoprecipitation followed by se-quencing(DRIP‐seq),we identified 8,932 R‐looppeaks that overlapped with 181 circRNAs and 672AS ***,several SDX‐related circRNAsoverlapped with R‐loop peaks,pointing to theirpossible roles in modulating AS in ***,overexpressing circ‐IRX7increased the levels ofR‐loop structures and decreased the frequency ofintron retention in *** provides a valuable R‐loop atlas resourceand uncovers the interplay between circRNAs andAS in SDX ***.

关键词： alternative splicing circular RNA R‐loop Populustrichocarpa stem‐differentiating xylem

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空间导航参考系研究进展

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空军航空医学 2024年第2期41卷 150-154页

作者：常琳丽白婉麒王会会张彦海迟丽屹空军军医大学空军第九八六医院神经内科西安710001 陕西中医药大学第二临床医学院咸阳712000 空军军医大学空军第九八六医院第一门诊部西安710001

空间导航是基于自身位置和外在环境构建复杂的认知地图,从而实现正确导航的过程。人类将同种异体中心和自我中心2种不同的参考系相结合,进行路线规划,导航到正确的目的地。空间导航能力与飞行安全密切相关,近几十年来国内外空军一直在... 详细信息

空间导航是基于自身位置和外在环境构建复杂的认知地图,从而实现正确导航的过程。人类将同种异体中心和自我中心2种不同的参考系相结合,进行路线规划,导航到正确的目的地。空间导航能力与飞行安全密切相关,近几十年来国内外空军一直在对飞行空间定向障碍进行研究和实践,但至今因飞行空间定向障碍造成的严重飞行事故仍然非常严重,也是A级飞行事故的主要原因。笔者综述不同空间导航参考系相关脑区的激活信息以及空间参考系在导航中的作用,从而探究人类导航与空间参考系之间的关系。基于现有研究,使用合适手段,如静息态功能磁共振等,对脑区激活和信息传导进行进一步研究,可以为选择合适的人群从事飞行等空间导航相关工作提供依据,对为飞行员制定个性化的空间导航能力训练方案具有现实意义。

关键词：空间导航空间参考系同种异体中心自我中心

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A synthetic light-inducible photorespiratory bypass enhances photosynthesis to improve rice growth and grain yield

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Plant Communications 2023年第6期4卷 85-95页

作者： Huawei Xu huihui wang Yanwen Zhang Xiaoyi Yang Shufang Lv Dianyun Hou Changru Mo Misganaw Wassie Bo Yu Tao Hu College of Agriculture Henan University of Science and TechnologyLuoyang 471000China CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenThe Innovative Academy of Seed DesignChinese Academy of SciencesWuhan 430074China Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems College of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730020China

Bioengineering of photorespiratory bypasses is an effective strategy for improving plant productivity by modulating *** previouswork,two photorespiratory bypasses,theGOC andGCGT bypasses,increased photosynthetic rates but decreased seed-setting rate in rice(Oryza sativa),probably owing to excess photosynthate accumulation in the *** solve this bottleneck,we successfully developed a newsynthetic photorespiratory bypass(called theGMAbypass)in rice chloroplasts by introducing Oryza sativa glycolate oxidase 1(OsGLO1),Cucurbita maxima malate synthase(CmMS),and Oryza sativa ascorbate peroxidase7(OsAPX7)into the rice genome using a high-efficiency transgene stacking *** the GOC and GCGT bypass genes driven by constitutive promoters,OsGLO1 in GMA plants was driven by a light-inducible Rubisco small subunit promoter(pRbcS);its expression dynamically changed in response to light,producing a more moderate increase in *** rates were significantly increased inGMA plants,and grain yieldswere significantly improved under greenhouse and field *** GMA rice showed no reduction in seed-setting rate under either test condition,unlike previous photorespiratory-bypass rice,probably reflecting proper modulation of the photorespiratory ***,these results imply that appropriate engineering of the GMA bypass can enhance rice growth and grain yield without affecting seed-setting rate.

关键词： photorespiratory bypass photosynthesis rice grain yield seed-setting rates

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Profiling of circular RNA N^(6)-methyladenosine in moso bamboo(Phyllostachys edulis)using nanopore-based direct RNA sequencing

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Journal of Integrative Plant Biology 2020年第12期62卷 1823-1838页

作者： Yongsheng wang huihui wang Feihu Xi Huiyuan wang Ximei Han Wentao Wei Hangxiao Zhang Qianyue Zhang Yushan Zheng Qiang Zhu Markus V.Kohnen Anireddy S.N.Reddy Lianfeng Gu Basic Forestry and Proteomics Research Center College of life scienceFujian Agriculture and Forestry UniversityFuzhou 350002China Basic Forestry and Proteomics Research Center College of ForestryFujian Agriculture and Forestry UniversityFuzhou 350002China College of Forestry Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology and Program in Cell and Molecular Biology Colorado State UniversityFort CollinsColoradoUSA

N6-methyladenosine(m^(6)A)is a prevalent modification in messenger RNAs and circular RNAs that play important roles in regulating various aspects of RNA ***,the occurrence of the m^(6)A modification in plant circular RNAs has not been reported.A widely used method to identify m^(6)A modifications relies on m^(6)A-specific antibodies followed by next-generation sequencing of precipitated RNAs(MeRIP-Seq).However,one limitation of MeRIP-Seq is that it does not provide the precise location of m^(6)A at single-nucleotide *** more recent sequencing techniques such as Nanopore-based direct RNA sequencing(DRS)can overcome such limitations,the technology does not allow sequencing of circular RNAs,as these molecules lack a poly(A)***,we developed a novel method to detect the precise location of m^(6)A modifications in circular RNAs using Nanopore *** first enriched our samples for circular RNAs,which we then fragmented and sequenced on the Nanopore platform with a customized *** this method,we identified 470 unique circular RNAs from DRS reads based on the back-spliced junction *** exonic circular RNAs,about 10%contained m^(6)A sites,which mainly occurred around acceptor and donor splice *** study demonstrates the utility of our antibody-independent method in identifying total and methylated circular RNAs using Nanopore *** method has the additional advantage of providing the exact location of m^(6)A sites at single-base resolution in circular RNAs or linear transcripts from non-coding RNA without poly(A)tails.

关键词： circular precise overcome

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Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing

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Phyton-International Journal of Experimental Botany 2023年第4期92卷 1275-1287页

作者： Luli Li Nigel G.Halford huihui wang Yingjie Zong Zhenzhu Guo Ruiju Lu Chenghong Liu Zhiwei Chen Shanghai Key Laboratory of Agricultural Genetics and Breeding Biotechnology Research InstituteShanghai Academy of Agricultural SciencesShanghai201106China College of Fisheries and Life Science Shanghai Ocean UniversityShanghai201306China Rothamsted Research HarpendenAL52JQUK Qingdao OE Biotechnology Company Limited Qingdao266100China

Barley(Hordeum vulgare L.)is an important economic crop for food,feed and industrial raw *** the present research,112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing(GBS),and the genetic diversity and population structure were *** results showed that 210,268 Single Nucleotide Polymorphisms(SNPs)were present in total,and the average poly-morphism information content(PIC)was *** diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups,with morphological traits of row-type and adherence of the hulls the main distinguishing factors between *** with similar or duplicated names were also investigated according to their genetic backgrounds and seed *** study provided valuable information on barley landraces from the Shanghai region,and showed that all these barley landraces should be protected and used for future breeding programs.

关键词： Hordeum vulgare L cluster analysis population structure SNP markers

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基于静息态功能磁共振成像的空间导航脑功能连接研究

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中国医学装备 2024年第4期21卷 28-33页

作者：常琳丽张彦海王会会白婉麒迟丽屹姚钦空军军医大学空军第九八六医院神经内科西安710000 陕西中医药大学第二临床医学院咸阳712000 空军军医大学空军第九八六医院第一门诊部西安710000 解放军陆军边海防学院西安710108 延安大学咸阳医院神经内科咸阳712000 空军特色医学中心北京100142

目的:探讨不同空间导航能力人群全脑各个脑区之间功能连接(FC)的特点,揭示空间导航可能的神经影像学机制。方法:前瞻性选取2020年9月至2021年9月解放军陆军边海防学院的100名大学生进行空间导航能力的测试,将测试结果优秀的前25名大学... 详细信息

目的:探讨不同空间导航能力人群全脑各个脑区之间功能连接(FC)的特点,揭示空间导航可能的神经影像学机制。方法:前瞻性选取2020年9月至2021年9月解放军陆军边海防学院的100名大学生进行空间导航能力的测试,将测试结果优秀的前25名大学生纳入空间导航能力极好(GN)组,测试结果最差的25名纳入空间导航能力极差(PN)组,采集两组的脑部静息态功能磁共振成像(fMRI)及数据。计算GN组和PN组之间以左侧眶部额上回、左侧枕下回、右侧枕中回为感兴趣区域(ROI)与大脑中其他脑区FC值的差异。结果:相较于GN组,PN组某些脑区FC值减弱。所减弱的脑区主要包括左侧眶部额上回与左侧顶骨下方除了缘上回和角回的脑回、左侧缘上回。左侧枕下回与双侧舌回、双侧矩状裂及其周围皮层。右侧枕中回与双侧楔叶、双侧楔前叶、双侧扣带回中部及其侧面环绕的脑回、右侧矩状裂及其周围皮层。结论:正常人群间的空间导航能力存在显著差异,并且空间导航GN组与空间导航PN组多个与情绪记忆及视觉信息处理相关的脑区之间FC值存在差异,可为揭示空间导航相关的神经机制提供依据。

关键词：空间导航静息态功能磁共振成像功能连接

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基于用户侧无功就地补偿装置的研制

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农村电气化 2024年第4期 60-64页

作者：吴伟朱浩杰王辉辉朱雯雯张岩卉许骁国网安徽省电力有限公司休宁县供电公司安徽黄山245400

为提升100 kV·A及以下低压动力用户功率因数、电能质量以及用户用电设备的使用效率,降低台区线损,增强客户的电力获得感,降低供电企业的购电成本,优化电网负荷,研制一种投入成本低、安装维护简便的无功补偿装置。

关键词： 100kV·A及以下低压动力用户电能质量电力获得感无功补偿装置

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Evaluating quality of motion for unsupervised video object segmentation

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Optoelectronics Letters 2024年第6期20卷 379-384页

作者： CHENG Guanjun SONG huihui Jiangsu Key Laboratory of Big Data Analysis Technology Jiangsu Collaborative Innovation Center on Atmospheric Environment and Equipment TechnologyNanjing University of Information Science&TechnologyNanjing 210044China12

Current mainstream unsupervised video object segmentation(UVOS) approaches typically incorporate optical flow as motion information to locate the primary objects in coherent video frames. However, they fuse appearance and motion information without evaluating the quality of the optical flow. When poor-quality optical flow is used for the interaction with the appearance information, it introduces significant noise and leads to a decline in overall performance. To alleviate this issue, we first employ a quality evaluation module(QEM) to evaluate the optical flow. Then, we select high-quality optical flow as motion cues to fuse with the appearance information, which can prevent poor-quality optical flow from diverting the network's attention. Moreover, we design an appearance-guided fusion module(AGFM) to better integrate appearance and motion information. Extensive experiments on several widely utilized datasets, including DAVIS-16, FBMS-59, and You Tube-Objects, demonstrate that the proposed method outperforms existing methods.

关键词： Evaluating quality of motion for unsupervised video object segmentation

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10 kV配电网单相接地故障模拟仿真建模技术研究

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内蒙古电力技术 2024年第2期42卷 37-43页

作者：徐肃张瑞强刘轶宋志文郑岩李彬王辉辉内蒙古电力(集团)有限责任公司包头供电分公司内蒙古包头014030

为实现电力系统数字仿真平台全面、快速、经济地考核配电网接地故障处置算法,提出了一种10 kV配电网单相接地故障仿真建模方法,能够实现系统电容电流设计及校验、中性点接地方式切换控制、Z形接地变压器建模及参数校核。基于该建模方法... 详细信息

为实现电力系统数字仿真平台全面、快速、经济地考核配电网接地故障处置算法,提出了一种10 kV配电网单相接地故障仿真建模方法,能够实现系统电容电流设计及校验、中性点接地方式切换控制、Z形接地变压器建模及参数校核。基于该建模方法,在实时数字仿真系统(Real Time Digital Simulation System,RTDS)完成建模,并接入小电流接地选线装置进行试验验证。结果表明,所搭建的配电网仿真模型的关键参数满足设计预期,小电流接地选线装置选线正确。

关键词：配电网单相接地故障模拟实时数字仿真系统

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Simplified design of nonlinear damper parameters and seismic responses for long-span cable-stayed bridges with nonlinear viscous dampers

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Frontiers of Structural and Civil Engineering 2024年第7期18卷 1103-1116页

作者： huihui LI Lifeng LI Rui HU Meng YE College of Civil and Transportation Engineering Shenzhen UniversityShenzhen 518060China Guangdong Provincial Key Laboratory of Durability for Marine Civil Engineering Shenzhen UniversityShenzhen 518060China College of Civil Engineering Hunan UniversityChangsha 410082China

Viscous dampers are widely used as passive energy dissipation devices for long-span cable-stayed bridges for mitigation of seismic load-induced ***,complicated finite element(FE)modeling,together with repetitive and computationally intensive nonlinear time-history analyses(NTHAs)are generally required in conventional design *** streamline the preliminary design process,this paper developed a simplified longitudinal double degree of freedom model(DDFM)for single and symmetric twin-tower cable-stayed *** on the proposed simplified longitudinal DDFM,the analytical equations for the relevant mass-and stiffness-related parameters and longitudinal natural frequencies of the structure were derived by using analytical and energy *** of the relationship between the nonlinear viscous damper parameters and the equivalent damping ratio was achieved through the equivalent linearization ***,the analytical equations of longitudinal seismic responses for long-span cable-stayed bridges with nonlinear viscous dampers were *** on the developed simplified DDFM and suggested analytical equations,this paper proposed a simplified calculation framework to achieve a simplified design method of nonlinear viscous damper ***,the effectiveness and applicability of the developed simplified longitudinal DDFM and the proposed calculation framework were further validated through numerical analysis of a practical cable-stayed ***,the results indicated the following.1)For the obtained fundamental period and longitudinal stiffness,the differences between results of the simplified longitudinal DDFM and numerical analysis were only 2.05%and 1.5%,respectively.2)Relative calculation errors of the longitudinal girder-end displacement and bending moment of the bottom tower section of the bridge obtained from the simplified longitudinal DDFM were limited to less than 25%.3)The equivalent damping ratio of nonline

关键词： cable-stayed bridges viscous dampers simplified analytical model equivalent damping ratio seismic mitigation

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