检索结果-南通市图书馆

Journal of Genetics and Genomics 2024年第5期51卷 566-569页

作者： Meilin Jin Gang liu Jian Lu Zhiqiang Chen huihua Wang Taotao li Caihong Wei State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100193China College of Animal Science and Technology China Agricultural UniversityBeijing100193China National Animal Husbandry Service National Center of Preservation&Utilization of Animal Genetic ResourcesBeijing 100125China Glbizzia Biosciences Beijing 102629China

Copy number variations(CNVs)range in size from 50 base pairs(bp)to several megabases(Mb)and encompass various types of structural variations,such as insertions,deletions,duplications,and complex multiallelic events(MacDonald et al.,2014).These genomic alterations modulate phenotype by altering gene dosage and transcriptional regulation,and are key drivers of biological evolution and determinants of genetic diversity and phenotypic variation.In recent years,CNVs have been detected in the genomes of various livestock species(Yuan et al.,2021;Chen et al.,2022).Upadhyay et al.(2017)conducted a comprehensive analysis of CNV distribution in the European cattle genome and determined that the Kit gene is associated with color-sidedness in English longhorn cattle.Another study presented extensive genome-wide information on CNVs in wild,commercial,and local chicken breeds to gain an integrated understanding of the domestication process and exclusively identified the genes involved(Chen et al.,2022).

关键词： sided number dosage

Absent in melanoma 2 attenuates proliferation and migration and promotes apoptosis of human colorectal cancer cells by activating P38MAPK signaling pathway

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Oncology Research 2024年第2期32卷 353-360页

作者： ZHI ZHANG XIAOSONG li YING ZHANG HAO ZHU ZHENGUO QIAO YANG LU XIUWEI MI huihua CAO GENHAI SHEN SONGBING HE Department of General Surgery Suzhou Ninth People’s HospitalSuzhou Ninth Hospital Affiliated to Soochow UniversitySuzhouChina Medical College of Nantong University NantongChina Department of Gastroenterology Suzhou Ninth People’s HospitalSuzhou Ninth Hospital Affiliated to Soochow UniversitySuzhouChina Department of General Surgery The First Affiliated Hospital of Soochow UniversitySuzhouChina Department of Oncological Surgery Kunshan Traditional Hospital Affiliated to Nanjing University of Chinese MedicineKunshanChina

Colorectal cancer(CRC)stands among the top prevalent cancers worldwide and holds a prominent position as a major contributor to cancer-related mortality globally.Absent in melanoma 2(AIM2),a constituent of the interferoninducible hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats protein family,contributes to both cancer progression and inflammasome activation.Despite this understanding,the precise biological functions and molecular mechanisms governed by AIM2 in CRC remain elusive.Consequently,this study endeavors to assess AIM2’s expression levels,explore its potential antitumor effects,elucidate associated cancer-related processes,and decipher the underlying signaling pathways in CRC.Our findings showed a reduced AIM2 expression in most CRC cell lines.Elevation of AIM2 levels suppressed CRC cell proliferation and migration,altered cell cycle by inhibiting G1/S transition,and induced cell apoptosis.Further research uncovered the participation of P38 mitogen-activated protein kinase(P38MAPK)in AIM2-mediated modulation of CRC cell apoptosis and proliferation.Altogether,our achievements distinctly underscored AIM2’s antitumor role in CRC.AIM2 overexpression inhibited proliferation and migration and induced apoptosis of CRC cells via activating P38MAPK signaling pathway,indicating AIM2 as a prospective and novel therapeutic target for CRC.

关键词： Absent in melanoma 2 Proliferation Migration Apoptosis P38MAPK Colorectal cancer

Changes in the gut microbiota of osteoporosis patients based on 16S rRNA gene sequencing:a systematic review and meta-analysis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第12期23卷 1002-1013页

作者： Rui HUANG Pan liU Yiguang BAI Jieqiong HUANG Rui PAN huihua li Yeping SU Quan ZHOU Ruixin MA Shaohui ZONG Gaofeng ZENG College of Public Hygiene of Guangxi Medical University Nanning 530021China Department of Spine Osteopathic the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Department of Orthopaedics the Third Affiliated Hospital of Xinxiang Medical UniversityNanning 530022China Department of Orthopaedics Nanchong Central Hospitalthe Second Clinical Institute of North Sichuan Medical CollegeXinxiang 45300China Department of Wound Repair the First People’s Hospital of NanningNanchong 637000China

Background:Osteoporosis(OP)has become a major public health issue,threatening the bone health of middle-aged and elderly people from all around the world.Changes in the gut microbiota(GM)are correlated with the maintenance of bone mass and bone quality.However,research results in this field remain highly controversial,and no systematic review or meta-analysis of the relationship between GM and OP has been conducted.This paper addresses this shortcoming,focusing on the difference in the GM abundance between OP patients and healthy controls based on previous 16S ribosomal RNA(rRNA)gene sequencing results,in order to provide new clinical reference information for future customized prevention and treatment options of OP.Methods:According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA),we comprehensively searched the databases of Pub Med,Web of Science,Embase,Cochrane library,and China National Knowledge Infrastructure(CNKI).In addition,we applied the R programming language version 4.0.3 and Stata 15.1 software for data analysis.We also implemented the Newcastle-Ottawa Scale(NOS),funnel plot analysis,sensitivity analysis,Egger’s test,and Begg’s test to assess the risk of bias.Results:This research ultimately considered 12 studies,which included the fecal GM data of 2033 people(604 with OP and 1429 healthy controls).In the included research papers,it was observed that the relative abundance of Lactobacillus and Ruminococcus increased in the OP group,while the relative abundance for Bacteroides of Bacteroidetes increased(except for Ireland).Meanwhile,Firmicutes,Blautia,Alistipes,Megamonas,and Anaerostipes showed reduced relative abundance in Chinese studies.In the linear discriminant analysis Effect Size(LEfSe)analysis,certain bacteria showed statistically significant results consistently across different studies.Conclusions:This observational meta-analysis revealed that changes in the GM were correlated with OP,and variations in some advantageous GM might involve regional differences.

关键词： Osteoporosis Microbiome Intestinal 16S ribosomal RNA(rRNA)sequencing

基于D-(-)-/L-(+)-对羟基苯甘氨酸的两对手性钴配合物的合成、晶体结构和电化学识别

在线全文

学校读者我要写书评

暂无评论

无机化学学报 2024年第1期40卷 232-246页

作者：曹梦茹姜国远李宏利李思浓宋会花河北师范大学化学与材料科学学院河北省有机功能分子重点实验室石家庄050024

采用常温溶液挥发法,以D-(-)-/L-(+)-对羟基苯甘氨酸(D-/L-Hhpg)为主配体,2种含氮吡啶配体4,4'-联吡啶(4,4'-bipy)和5,5'-二甲基-2,2'-联吡啶(5,5'-BM-2,2'-bipy)为辅助配体,与Co Cl_(2)·6H_(2)O反应合成了2对手性配合物{[Co(D-hpg)(4,4'-bipy)(H_(2)O)]Cl·H_(2)O}_(n )(1-D)、{[Co(L-hpg)(4,4'-bipy)(H_(2)O)]Cl·H_(2)O}_(n )(1-L)、[Co(D-hpg)_(2)(5,5'-BM-2,2'-bipy)]Cl·5.5H_(2)O (2-D)、[Co(L-hpg)_(2)(5,5'-BM-2,2'-bipy)]Cl·5.5H_(2)O (2-L)。通过单晶X射线衍射、元素分析、红外光谱、粉末X射线衍射等多种测试方法对其结构进行分析和表征。配合物的单晶X射线衍射数据表明,配合物1-D和1-L属于单斜晶系,P2_(1)手性空间群,分别呈现1D左手螺旋链和右手螺旋链,通过4,4'-bipy分子扩展为2D网状矩形格子结构。配合物2-D属于单斜晶系,P2_(1)手性空间群,为0D小分子,在氢键的作用下形成1D超分子双链,并以ABAB形式在a轴方向堆积排布。这些配合物的结构差异归因于辅助配体和Hhpg配位模式的影响。此外,还研究了配合物的电化学性质。配合物1-D表现出电化学可逆的氧化还原行为,并可作为电化学传感器用于有效地检测组氨酸对映体和定量测定组氨酸混合物中的对映体过量。

关键词： D-(-)-/L-(+)-对羟基苯甘氨酸手性配合物晶体结构电化学识别

Mobile technology:Usage and perspective of patients and caregivers presenting to a tertiary care emergency department

在线全文

学校读者我要写书评

暂无评论

World Journal of Emergency Medicine 2020年第1期11卷 5-11页

作者： Zhenghong liu Mingwei Ng Dinesh V.Gunasekeran huihua li Kishanti Ponampalam R Ponampalam SingHealth Emergency Medicine Residency Programme Singapore Health ServicesSingapore Ministry of Health Holdings Singapore Health Services Research Unit Singapore General HospitalSingapore University of Newcastle Australia Department of Emergency Medicine Singapore General HospitalSingapore

BACKGROUND:Developments in information technology(IT)have driven a push in healthcare innovation in the emergency department(ED).Many of these applications rely on mobile technology(MT)such as smartphones but not everyone is comfortable with MT usage.Our study aims to characterize the technology usage behavior of users in the ED so as to guide the implementation of IT interventions in the ED.METHODS:A cross-sectional survey was conducted in the emergency department of a tertiary hospital.Patients and their caregivers aged 21 and above were recruited.The survey collected demographic information,technology usage patterns,and participant reported comfort level in the usage of MT.We performed descriptive statistics and multivariate logistic regression to identify factors differentially associated with comfort in usage of MT.RESULTS:A total of 498 participants were recruited,and 299(60%)were patients.English was the most commonly written and read language(66.9%)and 64.2%reported a comfort level of 3/5 or more in using MT.Factors that were associated with being comfortable in using MT include having a tertiary education,being able to read and write English,as well as being a frequent user of IT.Caregivers were more likely to display these characteristics.CONCLUSION:A large proportion of ED patients are not comfortable in the usage of MT.Factors that predicted comfort level in the usage of MT were common amongst caregivers.Future interventions should take this into consideration in the design of MT interventions.

关键词： Mobile health technology Information technology Emergency department

公共价值视域下PPP项目回报机制决策研究:基于PSO-TRP模型

在线全文

学校读者我要写书评

暂无评论

铁道科学与工程学报 2024年第4期21卷 1634-1644页

作者：石世英赵方方李瑚均陈辉华胡晓珂河南理工大学土木工程学院河南焦作454003 中南大学土木工程学院湖南长沙410075 河海大学商学院江苏南京211100

回报机制是PPP项目的核心属性,政府作为PPP项目中公共服务合作生产的关键决策主体,其回报机制的决策逻辑关系到PPP项目公共性与经济效率的平衡,这也是直接影响PPP项目成败的关键问题。首先,综合考虑PPP项目公共性、参照点效应和边际效... 详细信息

回报机制是PPP项目的核心属性,政府作为PPP项目中公共服务合作生产的关键决策主体,其回报机制的决策逻辑关系到PPP项目公共性与经济效率的平衡,这也是直接影响PPP项目成败的关键问题。首先,综合考虑PPP项目公共性、参照点效应和边际效用递减规律,提出研究假设。其次,引入三参照点理论构建PPP项目回报机制决策模型并分析其约束条件。最后,利用粒子群算法对政府在预算约束和底线逻辑情境中如何选择回报机制获取最大效用的决策问题进行案例模拟、情景仿真与方案甄选。研究发现:1)PPP项目中政府感知价值随着目标的升高而先升高后降低,呈倒“U”型分布,感知价值和努力水平随底线升高而升高,最优位置即为公共价值达到最大化。2)当政府底线一定、目标较高时,政府完全或部分将公共资源让渡给社会资本主体,选择使用者付费或可行性缺口补助模式。3)当政府心理权重值发生改变时,成功权重越大,在最优目标范围内主体会加大自身的投入程度,选择基于公共价值的冒险行为;当失败权重越大,决策主体会减少自身的投入程度,选择基于公共价值的保底行为。研究立足于公共价值视域并引入三参照点理论,构建了基于粒子群算法的PPP项目回报机制决策模型,为PPP项目政府决策研究提供了新思路,同时也为维持PPP项目公共性和实现公共价值提供了参考。

关键词： PPP项目回报机制公共价值三参照点理论底线思维粒子群算法

在线全文

学校读者我要写书评

暂无评论

中国公民海外被侵害犯罪司法应对困境与进路

中国刑警学院学报 2024年第2期 95-104页

作者：任惠华李轩西南政法大学刑事侦查学院重庆401120

随着全球一体化进程的不断加速,从事跨境生产经营活动的中国公民日渐成为国家海外利益的重要载体,同时中国公民海外被侵害犯罪问题也变得愈发突出。通过对2016年以来87起中国公民海外被侵害犯罪的实证研究,分析应对海外公民被侵害犯罪... 详细信息

随着全球一体化进程的不断加速,从事跨境生产经营活动的中国公民日渐成为国家海外利益的重要载体,同时中国公民海外被侵害犯罪问题也变得愈发突出。通过对2016年以来87起中国公民海外被侵害犯罪的实证研究,分析应对海外公民被侵害犯罪时所面临的治理难点与司法困境,从突破创新司法管辖理念、拓展延伸国际司法合作、丰富完善司法取证手段、全面提升司法治理能力方面,提出体系化应对中国公民海外被侵害犯罪的对策组合,构建全域联动、立体高效的中国公民海外被侵害犯罪司法应对体系,推动中国海外公民安全保护战略的有效实施。

关键词：海外利益保护海外公民被侵害犯罪刑事管辖权海外调查取证国际刑事司法合作

基于GIS的城市应急避难场所空间区划——以成都市主城区为例

在线全文

学校读者我要写书评

暂无评论

中国安全生产科学技术 2024年第2期20卷 153-160页

作者：张小莹江惠华吕伟李承旭马剑海南科技职业大学财经学院海南海口571126 武汉理工大学安全科学与应急管理学院湖北武汉430070 西南交通大学交通运输与物流学院四川成都611756

为提升灾后城市整体应急避难服务能力,在综合考虑人口分布、交通网络、服务容量、建设成本和路网可达性等多因素的基础上,运用GIS空间分析技术,对四川省成都市主城区开展应急避难空间区划研究,提出高效、公平和经济的应急避难场所空间... 详细信息

为提升灾后城市整体应急避难服务能力,在综合考虑人口分布、交通网络、服务容量、建设成本和路网可达性等多因素的基础上,运用GIS空间分析技术,对四川省成都市主城区开展应急避难空间区划研究,提出高效、公平和经济的应急避难场所空间区划方案,形成1套科学的方案制定方法和流程。研究结果表明:成都市主城区可划分成10个应急避难分区,并确立14个最佳避难场所来满足区域内的应急避难需求;同时对实验外国语学校、丽都公园进行容量扩建,并升级浣花溪公园周边交通配套设施来优化应急避难场所空间区划,以此提升成都市主城区整体应急避难服务能力。研究结果可为城市人口转移避难安置工作规划和决策实施提供技术支持。

关键词：应急避难场所服务能力空间区划 GIS 位置分配

明串珠菌与乳植杆菌复合发酵对低盐泡萝卜品质的影响

在线全文

学校读者我要写书评

暂无评论

食品工业科技 2024年第11期45卷 102-109页

作者：赵勇刘毕琴陈骏飞汤回花唐蓉史巧阚欢李宏西南林业大学生命科学学院云南昆明650224 云南农业科学院农产品加工研究所云南昆明650223 云南农业大学云南昆明650201

为研究不同乳酸菌对泡萝卜品质的影响,采用肠膜明串珠菌(Leuconostoc mesenteroides)AP7、戊糖乳植杆菌(Lactiplantibacillus pentosus)LP10及AP7-LP10双菌复合制备泡萝卜,分析发酵过程理化指标、色差、质构特性变化和感官指标。结果表... 详细信息

为研究不同乳酸菌对泡萝卜品质的影响,采用肠膜明串珠菌(Leuconostoc mesenteroides)AP7、戊糖乳植杆菌(Lactiplantibacillus pentosus)LP10及AP7-LP10双菌复合制备泡萝卜,分析发酵过程理化指标、色差、质构特性变化和感官指标。结果表明:接菌组pH、还原糖含量下降速率和总酸、乳酸升高速率优于自然发酵组(P<0.05);接菌组亚硝酸盐含量低,无亚硝峰的出现,果胶酶活性保持较低水平;AP7-LP10组和AP7组L高于其余2组(P<0.05),褐变指数和多酚氧化酶活性保持在较低水平;发酵7 d,AP7-LP10组硬度(70.92±3.94 N)高于其他组(P<0.05);AP7-LP10感官评分最高(92±2.11)。综上,接种乳酸菌泡萝卜成熟快,安全性高,AP7-LP10双菌复合有助于提升泡萝卜品质。

关键词：乳酸菌泡萝卜接种发酵理化指标感官品质

维普期刊数据库博看期刊

柱前衍生-气相色谱-质谱联用法同时测定茯苓中的可溶性糖与三萜酸

在线全文

学校读者我要写书评

暂无评论

厦门大学学报（自然科学版） 2024年第3期63卷 532-540页

作者：陈菲李惠华陈淳吴承文明艳林福建省亚热带植物研究所福建省亚热带植物生理生化重点实验室福建厦门361006 尤溪县林业局福建尤溪365100 国家农作物国外引种隔离检疫基地(厦门) 厦门华侨亚热带植物引种园厦门市植物引种检疫与植物源产物重点实验室福建厦门361002

[目的]通过建立柱前衍生-气相色谱-质谱(GC-MS)法实现茯苓(Poria cocos)各成分的快速高效分离检测,为茯苓有效成分的分析提供新方法并为茯苓原料品质判断提供参考.[方法]采用超声辅助提取法,以反油酸为内标物,甲氧胺(MOX)和N-甲基-N-(... 详细信息

[目的]通过建立柱前衍生-气相色谱-质谱(GC-MS)法实现茯苓(Poria cocos)各成分的快速高效分离检测,为茯苓有效成分的分析提供新方法并为茯苓原料品质判断提供参考.[方法]采用超声辅助提取法,以反油酸为内标物,甲氧胺(MOX)和N-甲基-N-(三甲基硅烷)三氟乙酰胺[含1%(质量分数)三甲基氯硅烷](MSTFA+1%TMCS)为肟化-硅烷化衍生试剂,采用GC-MS同时检测茯苓中的果糖、葡萄糖、海藻糖、阿拉伯糖醇、甘露糖醇、山梨糖醇、麦芽糖醇、茯苓酸、茯苓新酸A和茯苓新酸B.[结果]优化后的衍生条件为:200μL茯苓的甲醇提取液氮气吹干后,加入50μL MOX,于40℃反应2 h,然后加入250μL(MSTFA+1%TMCS),于40℃下反应30 min.衍生后的目标化合物在40 min内完成分离分析,线性关系良好,相关系数均在0.9935以上,该方法的定量限为0.008~1.000μg/mL,检测限为0.003~0.300μg/mL.茯苓样品加标回收率为82.0%~122.4%,相对标准偏差为1.05%~4.43%.[结论]本方法样品用量少,结果稳定且灵敏度高,可用于检测茯苓样品中可溶性糖和微量的三萜酸类化合物,同时也为检测其他样品中的三萜酸类化合物提供更多的选择.

关键词：衍生气相色谱-质谱法可溶性糖三萜酸茯苓