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Single-level dynamic spiral CT of hepatocellular carcinoma:Correlation between imaging features and density of tumor microvessels

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World Journal of Gastroenterology 2004年第1期10卷 67-72页

作者： Wei-XiaChen Peng-QiuMin BinSong Bong-LiangXiao YanLiu Ying-huige DepartmentofRadiology WestChinaHospitalSichuanUniversityChengdu610041SichuanProvinceChina DepartmentofToxicologyandPathology SchoolofPublicHealthSichuanUniversityCh-engdu610041SichuanProvinceChina DepartmentofRadiology WestChinaHospitalSichuanUniversitynowworkinginRijinHospitalShanghaiChina DepartmentofRadiology WestChinaHospitalSichuanUniversitynowworkinginHenanProvinceHospitalHenanProvinceChina

AIM: To investigate the correlation of enhancement features of hepatocellular carcinoma (HCC) revealed by single-level dynamic spiral CT scanning (DSCT) with tumor microvessel density (NVD), and to determine the validity of DSCT in assessing in vivo tumor angiogenic activity of HCC. METHODS: Twenty six HCC patients were diagnosed histopathologically. DSCT was performed for all patients according to standard scanning protocol. Time-density curves were generated, relevant curve parameters were measured,and gross enhancement morphology was analyzed. Operation was performed to remove HCC lesions i to 2 weeks following CT scan. Histopathological slides were carefully prepared for the standard F8RA immunohistochemical staining and tumor microvessel counting. Enhancement imaging features of HCC lesions were correlatively studied with tumor MVD and its intra-tumor distribution characteristics. RESULTS: On DSCT images of HCC lesions, three patterns of time-density curve and three types of gross enhancement morphology were recognized. Histomorphologically, the distribution of positively stained tumor endothelial cells within tumor was categorized into 3 types. Curve parameters such as peak enhancement value and contrast enhancement ratio were significantly correlated with tumor tissue MVD (/=0.508 and /=0.423, P<0.01 and P<0.05 respectively). Both the pattern of time-density curve and the gross enhancement morphology of HCC lesions were also correlated with tumor MVD, and reflected the distributive features of tumor microvessels within HCC lesions. Correlation between the likelihood of intrahepatic metastasis of HCC lesions with densely enhanced pseudocapsules and rich pseudocapsular tumor MVD was ***: Enhancement imaging features of HCC lesions on DSCT scanning are correlated with tumor MVD,and reflect the intra-tumor distribution characteristics of tumor microvessels. DSCT is valuable in assessing the angiogenic activity and tumor neovascularity of HCC patients

关键词：动态螺旋CT 肝细胞癌微血管密度影像学诊断

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Recent advances in DNA glycosylase assays

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Chinese Chemical Letters 2022年第8期33卷 3603-3612页

作者： Lili Wang huige Zhang Wei Chen Hongli Chen Jianxi Xiao Xingguo Chen State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou 730000China

Genomic deoxyribonucleic acid(DNA)is selected as the ideal carrier for preserving and transmitting the genetic information over the course of ***,the genomic DNA is constantly exposed to various endogenous and environmental threats,causing a diversity of damaged bases,lesions,mismatches and base-pair modifications in the genome,eventually leading to genomic instability and *** excision repair(BER)is the most important repair mechanism,repairing a variety of DNA damages arising from oxidation,alkylation,methylation,deamination,and hydrolysis *** glycosylases are responsible for initiating the first step of the BER pathway through cleaving the N-glycosidic bond between the damaged base and the DNA ***,abnormal DNA glycosylases are associated with a variety of diseases such as cancer,cardiovascular disease,neurological disease and inflammation,suggesting the important role of DNA glycosylases in cancer diagnosis and ***,it is highly desirable to monitor the activity of DNA glycosylases,gaining a deep understanding of the restoration process of damaged DNA and clinical ***,a series of novel DNA glycosylases detection methods with excellent performance have been *** this minireview,we summarize the recent advances in DNA glycosylase assays including amplification-free assay and amplification-assisted ***,a brief introduction of amplification-free assay for DNA glycosylase is ***,amplification-assisted assays for DNA glycosylases are discussed in ***,the conclusion and prospects of the directions of DNA glycosylase assays are provided.

关键词： DNA glycosylases Base excision repair Polymerase chain reaction Isothermal signal amplification Nanomaterial-based biosensors

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2型糖尿病患者发生新型冠状病毒感染的危险因素分析

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华夏医学 2024年第3期37卷 215-220页

作者：欧翔刘丽莎汤石林邵挥戈南华大学衡阳医学院附属长沙中心医院内分泌科长沙410004 南华大学衡阳医学院附属长沙中心医院检验科长沙410004 南华大学附属第一医院重症医学科衡阳421001

目的探讨2型糖尿病患者发生新型冠状病毒感染的危险因素。方法选取未合并新型冠状病毒感染的2型糖尿病患者78例(未感染组),合并新型冠状病毒感染的2型糖尿病患者24例(感染组),比较两组的年龄、体质量指数、糖尿病病程、收缩压、舒张压... 详细信息

目的探讨2型糖尿病患者发生新型冠状病毒感染的危险因素。方法选取未合并新型冠状病毒感染的2型糖尿病患者78例(未感染组),合并新型冠状病毒感染的2型糖尿病患者24例(感染组),比较两组的年龄、体质量指数、糖尿病病程、收缩压、舒张压、血脂、白细胞数、中性粒细胞数、空腹血糖、餐后2 h血糖、空腹C肽、餐后2 h C肽、糖化血红蛋白、β-羟丁酸,并分析上述因素与新型冠状病毒感染的相关性。结果感染组的总胆固醇显著低于未感染组,差异有统计学意义(P<0.05);感染组的糖化血红蛋白和β-羟丁酸显著高于未感染组(P<0.05)。Spearman相关分析结果显示,新型冠状病毒感染与空腹血糖、糖化血红蛋白、收缩压、β-羟丁酸成正相关(P<0.05),与总胆固醇和餐后2 h C肽成负相关(P<0.05)。Logistic回归分析结果显示,总胆固醇和糖化血红蛋白是2型糖尿病患者合并新型冠状病毒感染的重要影响因素(P<0.05)。结论空腹血糖、糖化血红蛋白、收缩压、β-羟丁酸水平升高以及总胆固醇和餐后2 h C肽水平降低可能是2型糖尿病患者发生新型冠状病毒感染的危险因素,控制相关因素可减少2型糖尿病患者感染新型冠状病毒的风险。

关键词： 2型糖尿病新型冠状病毒感染危险因素

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Thermal Stability,Thermal Decomposition and Mechanism Analysis of Cycloaliphatic Epoxy/4,4'-dihydroxydiphenylsulfone/Aluminum Complexes Latent Resin Systems

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Journal of Wuhan University of Technology(Materials Science) 2012年第6期27卷 1061-1067页

作者：吴松全 LIU Shaozhu 刘宇艳 ZHU Qi WEI huige School of Chemical Engineering and Technology Harbin Institute of Technology

The thermal stability of latent resin systems, cycloaliphatic epoxy/4,4＇- dihydroxydiphenylsulfone/aluminum complexes, was investigated by dynamic differential scanning calorimetry （DSC） analysis. Experiments were conducted under non-isothermal condition in a nitrogen atmosphere at the heating rate of 10, 20, 30 and 40 ℃/min, respectively. TG curves showed that, in the temperature range of 25 to 600 ℃, the stability of the resin systems could be enhanced by increasing the length of the aliphatic chain in the initiator. Both the Kissinger method and the Ozawa-Flyrm-Wall method were employed to calculate activation energies of the decomposition reaction, and the values obtained from the two methods were compared. Moreover, the corresponding reaction mechanism was identified by the Achar differential method and the Coats- Redfem integral method. The experimental results showed that these four methods were reliable and effective to study the kinetics of the thermal decomposition reaction; and the most probable thermal decomposition mechanism of the resin systems we proposed was found to comply with Mampel power law （m=1）.

关键词： epoxy resin aluminum complex phase separation thermal decomposition

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Enhanced plasma factor Ⅷ activity in mice via cysteine mutation using dual vectors

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Science China(Life Sciences) 2012年第6期55卷 521-526页

作者： ZHU FuXiang LIU ZeLong MIAO Jing QU huige CHI XiaoYan Life Science College of Ludong University Yantai 264025China

Hemophilia A is caused by a genetic mutation in coagulation factor VIII （FVIII） gene and gene therapy is considered to be a promising strategy for its treatment. We recently demonstrated that co-delivery of two vectors expressing M662C mutated heavy and D1828C mutated light chain genes of B-domain-deleted coagulation factor VIII （BDD-FVIII） leads to inter-chain disulfide cross-linking and improved heavy chain secretion in vitro. In this study, co-injection of both M662C and D1828C mutated BDD-FVIII gene expression vectors into mice resulted in increased heavy chain secretion and coagulation activity in plasma in vivo. Approximately （239＋_56） ng mL-1 above endogenous levels of transgenic FVIII heavy chain was found in mouse plasma using a chain-specific ELISA. For FVIII coagulation activity, approximately （1.09＋_0.25） IU mL-1 above en- dogenous levels were detected in co-injected transgenic mouse plasma using a chromogenic assay. These data demonstrate that inter-chain disulfide bonds likely increase heavy chain secretion and coagulation activity in the plasma of transgenic mice with an improved efficacy of a dual-vector delivery of BDD-FVIII gene. These findings support our ongoing efforts to develop a gene therapy for hemophilia A treatment using dual-AAV vectors.

关键词： coagulation factor VIII dual-vector gene delivery inter-chain disulfide bonding heavy chain secretion coagulation ac-tivity

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The effect of a secretion-enhanced heavy chain on improving intein-based dual-vector co-delivery of a full-length factor Ⅷ gene

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Chinese Science Bulletin 2011年第2期56卷 158-163页

作者： ZHU FuXiang YANG ShuDe LIU ZeLong MIAO Jing QU huige CHI XiaoYan College of Life Sciences Ludong University Yantai 264025 China

Treatment of hemophilia A by gene therapy is adversely affected by inefficient FVIII secretion and the large FVIII gene,which is difficult to package in the promising adeno-associated virus (AAV) *** secretion of FVIII is caused mainly by inefficient secretion of its heavy ***,we have employed a protein splicing-based dual-vector to co-transfer a B-domain-deleted FVIII (BDD-FVIII) gene,suggesting that the light chain,covalently ligated to a co-expressed heavy chain can improve the secretion of spliced ***,its level of secretion was affected by inefficient secretion the heavy ***,we studied the effect of a mutant heavy chain with L303E/F309S substitutions,which enhance FVIII secretion on the heavy chain itself and spliced FVIII when using a protein splicing-based split-delivery of a full-length FVIII *** vectors expressing Ssp DnaB intein-fused mutant heavy and light chains were transiently co-transfected into cultured COS-7 cells.A spliced FVIII protein was seen in co-transfected cells by Western blot *** heavy chain was secreted by cells transfected with the mutant heavy chain gene alone at (39±11) ng/mL and this secretion increased to (123±13) ng/mL when cells were co-transfected with the light chain gene,which was greater than the secretion of wild-type heavy *** amount of spliced FVIII in the culture supernatant of co-transfected cells was (86±14) ng/mL,with an activity of (0.61±0.08) IU/mL,which was greater than that of wild-type FVIII co-transfected *** FVIII and bioactivity were also detected in the combined culture supernatant of cells individually transfected with mutant heavy and light chain gene at a higher level than that of combined wild-type heavy and light chain *** suggested that the heavy chain with improved secretion markedly increased the efficacy of protein splicing-based split delivery of the full-length FVIII gene using a *** result

关键词：分泌作用基因传递因子和内含肽轻链载体基础蛋白质剪接

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Inter-chain disulfide bond improved protein trans-splicing increases plasma coagulation activity in C57BL/6 mice following portal vein FVIII gene delivery by dual vectors

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Science China(Life Sciences) 2013年第3期56卷 262-267页

作者： ZHU FuXiang LIU ZeLong WANG XiaoLei MIAO Jing QU huige CHI XiaoYan College of Life Science Ludong University Department of Hematology Yuhuangding Hospital

Protein trans-splicing based dual-vector factor VIII （FVIII） gene delivery is adversely affected by less efficiency of protein splicing. We sought to increase the amount of spliced FVIII protein and plasma coagulation activity in dual-vector FVIII transgene in mice by means of strengthening the interaction of inteins, protein splicing elements, thereby facilitating protein trans-splicing. Dual-vector delivery of the FVIII gene in cultured cells showed that replacement of Met226 in the heavy chain and Asp1828 in the light chain with Cys residues could facilitate inter-chain disulfide linking and improve protein trans-splicing, increasing the levels of spliced FVIII protein. In this study, C57BL/6 mice were coadministered dual vectors of intein-fused human FVIII heavy chain and light chain with Cys mutations via portal vein injection into the liver. Forty-eight hours post-injection, plasma was collected and analyzed for FVIII antigen concentration and coagulation activity. These mice showed increased circulating FVIII heavy chain polypeptide （442± 151 ng mL i vs. 305±103 ng mL-1） and coagulation activi- ty （1.46±0.37 IU mL i vs. 0.85±0.23 IU mL-1） compared with control mice co-administered dual vectors expressing the heavy and light chains of wild-type FVIII. Moreover, coagulation activity was similar to that of mice receiving a single vector ex- pressing FVIII （1.79_＋0.59 IU mL-l）. These findings indicate that improving protein trans-splicing by inter-chain disulfide bonding is a promising approach for increasing the efficacy of dual-vector based FVIII gene transfer.

关键词： coagulation factor VIII protein trans-splicing dual-vector gene delivery plasma coagulation activity

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Various patterns in dielectric barrier glow discharges simulated by a dynamic model

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Plasma Science and Technology 2019年第8期21卷 43-50页

作者： Xiaoxi DUAN Benqiong LIU huige ZHANG Ben LI and Jiting OUYANG Research Center of Laser Fusion China Academy of Engineering PhysicsMianyang 621900People’sRepublic of China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang621900People’s Republic of China School of Physics Beijing Institute of TechnologyBeijing100081People’s Republic of China

In this paper, various patterns of dielectric barrier glow discharge simulated by a phenomenological dynamic model are *** model is constructed based on the basic dynamic process of dielectric barrier glow discharge and involves the voltage-transfer characteristic as well as the lateral inhibition *** simulations, different driving voltage profiles are applied to achieve one or two pulsed discharges in each half-period and the corresponding pattern evolution is *** final stable patterns driven by a rectangular wave voltage organize simply as stationary striations or hexagonal *** patterns driven by a multi-step wave appear to be much more complicated, with complementary striations,concentric rings and square superlattices *** evolutions of these patterns from the initial uniform state are described and it is found that the spreading of the inhibition effect plays a key role in these *** numerical simulations in the present work are in excellent accordance with previous experiments and fluid *** dynamic model proves to be a convenient and promising approach to reproducing different pattern structures and pattern evolutions in dielectric barrier glow discharge systems.

关键词： dielectric barrier discharge voltage-transfer characteristic dynamic model pattern formation

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Dexamethasone, tetrahydrobiopterin and uncoupling of endothelial nitric oxide synthase

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Journal of Geriatric Cardiology 2015年第5期12卷 528-539页

作者： Silke Tobias Alice Habermeier Daniel Siuda Gisela Reifenberg Ning Xia Ellen I Closs Ulrich Forstermann huige Li Department of Pharmacology Johannes Gutenberg University Medical Center Mainz Germany

Objective To find out whether dexamethasone induces an uncoupling of the endothelial nitric oxide synthase （eNOS）. Methods ＆ Results A major cause of eNOS uncoupling is a deficiency of its cofactor tetrahydrobiopterin （BH4）. Treatment of human *** 926 endothelial cells with dexamethasone decreased mRNA and protein expression of both BH4-synthesizing enzymes： GTP cyclobydrolase I and dihydrofolate reductase. Consistently, a concentration- and time-dependent reduction of BH4, dihydrobiopterin （BH2） as well as BH4：BH2 ratio was observed in dexamethasone-treated cells. Surprisingly, no evidence for eNOS uncoupling was found. We then analyzed the expression and phosphorylation of the eNOS enzyme. Dexamethasone treatment led to a down-regulation of eNOS protein and a reduction of eNOS phosphorylation at serine 1177. A reduction of eNOS expression may lead to a relatively normal BH4： eNOS molar ratio in dexamethasone-treated cells. Because the BH4-eNOS stoichiometry rather than the absolute BH4 amount is the key determinant of eNOS functionality （i.e., coupled or uncoupled）, the down-regulation of eNOS may represent an explanation for the absence of eNOS uncoupling. Phosphorylation of eNOS at serine 1177 is needed for both the NO-producing activity of the coupled eNOS and the superoxide-producing activity of the uncoupled eNOS. Thus, a reduction of serine 1177 phosphorylation may render a potentially uncoupled eNOS hardly detectable. Conclusions Although dexamethasone reduces BH4 levels in endothelial cells, eNOS uncoupling is not evident. The reduction of NO production in dexamethasone-treated endothelial cells is mainly attributable to reduced eNOS expression and decreased eNOS phosphorylation at serine 1177.

关键词： Dexamethasone Endothelial cells eNOS uncoupling Nitric oxide synthase Reactive oxygen species Tetrahydrobiopterin

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基于现代学徒制和OBE理念的食品智能加工技术类课程建设探索

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粮油科学与工程 2024年第3期38卷 43-45页

作者：王娇刘玉青焦婷婷刘会阁漯河食品职业学院河南漯河462000

针对食品人才培养目标模糊、内容滞后、教学实施及评价方法单一等问题,漯河食品职业学院基于现代学徒制培养模式和OBE理念,结合食品产业特点,对食品智能加工技术类课程建设进行了探索。通过改进,形成了“一理念、二模式、三层级、三评... 详细信息

针对食品人才培养目标模糊、内容滞后、教学实施及评价方法单一等问题,漯河食品职业学院基于现代学徒制培养模式和OBE理念,结合食品产业特点,对食品智能加工技术类课程建设进行了探索。通过改进,形成了“一理念、二模式、三层级、三评价、四融合、八环节”的教学特色,教学效果良好,该研究成果可为其他食品高职院校培养技能型人才提参考。

关键词：现代学徒制 OBE理念食品智能加工技术课程建设

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