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Effect of pyridone agent on blood-retinal barrier in diabetic mice

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International Journal of Ophthalmology(English edition) 2017年第6期10卷 890-895页

作者： Si-Qi Xiong Hai-Bo Jiang hui-zhuo xu Xiao-Bo Xia Department of Ophthalmology Xiangya Hospital Central South University Changsha 410008 Hunan Province China

AIM： To evaluate the therapeutic effect of fluorofenidone on disrupted blood-retinal barrier in the diabetic mice and uncover its underlying mechanism. METHODS： db/db mice were randomly chosen for treatment with daily doses of fluorofenidone or placebo at 5-week-old, treatment continued until mice reach 24-week- old. Then, expression of transcriptiona factor insulin gene enhancer binding protein-1 （Islet-I） and vascular endothelial growth factor （VEGF） in murine retinas were evaluated. Retinal vascular permeability was assessed by examining the level of albumin in db/db murine retinas. Furthermore, the retinal vessel tight junction was estimated by checking the level of occludin in the murine retinal tissues. RESULTS： After occurrence of diabetic retinopthy in db/ db mice, expressions of transcritpional factor Islet-1 was found to be upregulated in db/db murine retinas compared with non-diabetic controls. Similar to expression pattern of Islet-l, VEGF were also demonstrated to be increased in retinas of db/db mice, which was accompanied by increased retinal vascular leakage and decreased tight junction protein level. Systemetic administration of fluorofenidone repaired broken retinal vascular tight junction by restoring occludin expression in db/db retinal tissue. Consequently, retinal vascular premeability were indicated to be reduced by examining the transudative albumin level in diabetic retinal tissues. Both Islet-1 and VEGF expression were inhibited in the retinas of db/db mice after treatment with fluorofenidone. CONCLUSION： Fluorofenidone significantly protectes retinal tight junction and reduces retinal vascular leakage. The phenomenon can be partially attributed to reducing overexpression of Islet-1 and VEGF in diabetic retinal tissues.

关键词： pyridone agent diabetic retinopathy blood-retinal barrier

Activation of the ERK 1/2 and STAT3 signaling pathways is required for 661W cell survival following oxidant injury

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International Journal of Ophthalmology(English edition) 2012年第2期5卷 138-142页

作者： Shu-Qian Dong hui-zhuo xu Xiao-Bo Xia Sha Wang Li-Xin Zhang and Shuang-Zhen Liu Department of Ophthalmology Xiangya Hospital of Central South University Changsha 410006 Hunan Province China

AIM: To evaluate the influence of hydrogen peroxide (H2O2) on mouse photoreceptor-derived 661W cell survival and to determine the effect of PD98059, an inhibitor for MEK1 (the direct upstream activator of ERK1/2), and S3I201, a STAT3-specific inhibitor on 661W cell survival after H2O2 exposure. METHODS: The mouse photoreceptor-derived 661W cells were cultured. 661W cells were treated for 12 hours with different concentrations (0, 0.25, 0.50, 0.75, 1mmol/L) of H2O2 and cell viability was determined by 3- (4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) (MTT) assay. 661W cells were treated with different concentrations H2O2 (0, 5, 10, 50, 500, 1000 mu mol/L) for 15 minutes or 1mmol/L H2O2 for different time points (0,5,10,15,30 minutes), and p-Tyr705-STAT3, STAT3, Phospho-p44/42 MAPK (Thr202/Tyr204), ERK1/2 were surveyed by immunoblot analysis. After treatment with 50 mu mol/L PD98059, or S3I201 for 1 hour, the inhibition efficiency of cell signal pathways was analyzed by immunoblot analysis and the effects of inhibitors on cell viability were determined by MTT. RESULTS: After treating with different concentrations of H2O2 for 12 hours, the cell viability of 661W cells decreased in concentration-dependent manner (P<0.05). Moreover, H2O2 induced phosphorylation of ERK1/2 and STAT3 in 661W cells (P <0.05). After pretreatment with 50 mu mol/L PD98059 or S3I201 for 1 hour, H2O2-induced phosphorylation of ERK1/2 or STAT3 was suppressed separately (P<0.05). Using PD98059 or S3I201 to inhibit ERK1/2 or STAT3 signal pathway, the cell viability of 661W cells decreased significantly (P<0.05). CONCLUSION: We demonstrated that the exposure of 661W cells to H2O2 increased the activation of ERK1/2 and STAT3 signal pathways. Activation of these pathways is required for 661W cell survival following oxidant injury.

关键词： 661W cells oxidant injury ERK1/2 STAT3 survival

Treatment of myopic foveoschisis via macular buckling and vitrectomy

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International Journal of Ophthalmology(English edition) 2017年第5期10卷 815-818页

作者： Si-Qi Xiong Hai-Bo Jiang Fang-Ling Li Yan-Xiu Li Jie Yang Xiao-Bo Xia hui-zhuo xu Department of Ophthalmology Xiangya Hospital Central South University Changsha 410008 Hunan Province China

The aim of the present study was to evaluate the efficacy and safety of the treatment of myopic foveoschisis patients using the macular buckling with L-shaped titanium plate and silicon sponge combined with vitrectomy. The data of the patients who underwent macular buckling combined with vitrectomy was collected. The study recorded the following parameters：best corrected visual acuity（BCVA）, axial length, intraocular pressure, central macular thickness, and the position of the titanium plate. Following the surgery, the BCVA of the included patients were improved, whereas the axial lengths were reduced followed by resolution of the foveoschisis compared with that noted prior to the operations. All patients had orbital CT examination and the results indicated that the titanium plates were appropriately placed and were not in contact with the optic nerve. Therefore, it is effective to treat myopic foveaschisis by macular buckling using the L-shaped titanium plate and silicon sponge in the presence of vitrectomy.

关键词： myopic foveoschisis macular buckling vitrectomy

Superhydrophobic and corrosion-resistant siloxane-modified MgAl-LDHs coatings on magnesium alloy prepared under mild conditions

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 450-463页

作者： Wenxi Zhang Zhangzelong zhuo Dan xu Liang Wu Zhihui Xie Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province College of Chemistry and Chemical EngineeringChina West Normal UniversityNanchong 637002China College of Materials Science and Engineering/National Engineering Research Center for Magnesium Alloys Chongqing UniversityChongqing 400044China

We have developed a superhydrophobic and corrosion-resistant LDH-W/PFDTMS composite coating on the surface of Mg *** composite comprised a tungstate-intercalated(LDH-W)underlayer that was grown at low temperature(relative to hydrothermal reaction conditions)under atmospheric pressure and an outer polysiloxane layer created from a solution containing perfluorodecyltrimethoxysilane(PFDTMS)using a simple immersion *** successful intercalation of tungstate into the LDH phase and the following formation of the polysiloxane layer were confirmed through X-ray diffraction(XRD),Fourier transform infrared(FTIR)spectroscopy,and X-ray photoelectron spectroscopy(XPS).The corrosion resistance of the LDH-W film,both before and after the PFDTMS modification,was evaluated using electrochemical impedance spectroscopy(EIS),Tafel curves,and immersion *** results showed that Mg coated with LDH-W/PFDTMS exhibited significantly enhanced corrosion protection compared to the unmodified LDHW film,with no apparent signs of corrosion after exposure to 3.5wt%NaCl solution for 15 ***,the LDH-W/PFDTMS coating demonstrated superior superhydrophobicity and self-cleaning properties against water and several common beverages,as confirmed by static contact angle and water-repellency *** results offer valuable insights into preparing superhydrophobic and corrosion-resistant LDH-based composite coatings on Mg alloy surfaces under relatively mild reaction conditions.

关键词： Mg alloy corrosion coating layered double hydroxide

景物平面—— 一种用于超文本浏览的可视模型

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计算机辅助设计与图形学学报 1998年第5期10卷 464-470页

作者：赵奂辉许卓群北京大学计算机科学与技术系

模拟人们浏览信息的物理习惯：即每个对象都有确定的空间位置，人们的视线能同时观察到多个对象，并随着思维的联想，注意力的不同而不断变化．提出一种用于超文本浏览的可视模型——景物平面模型：它是一个广阔的二维平面，其上的每个... 详细信息

模拟人们浏览信息的物理习惯：即每个对象都有确定的空间位置，人们的视线能同时观察到多个对象，并随着思维的联想，注意力的不同而不断变化．提出一种用于超文本浏览的可视模型——景物平面模型：它是一个广阔的二维平面，其上的每个对象都有一个具体的位置，还有一些浏览工具——取景器，使得用户能在视野所及的环境中按自己的意图组织观察到的对象，将人的联想思维记忆和视觉的观察有机地结合起来．它适合信息浏览，多媒体教育，远程资源共享合作．详细说明了这个景物平面模型，它的数据结构以及应用前景．

关键词：超文本可视模型景物平面可视对象多媒体

Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future

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Neural Regeneration Research 2025年第4期20卷 946-959页

作者： Zhiyuan Yin Jiahui Kang xuan Cheng hui Gao Shujia Huo Haiwei xu Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Southwest Eye HospitalSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased *** lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored ***,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various *** in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in ***,there remain issues that hinder improving reprogramming efficiency and ***,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming *** review describes recent progress in relatively successful Müller glia reprogramming *** also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关键词： cell fusion chemical small-molecules epigenetic extracellular matrix immune metabolic mice Müller glia neurodegenerative diseases reprogramming retina regeneration

重组慢病毒GV303 Rat IL-33体外转染原代大鼠骨髓间充质干细胞的研究

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国际眼科杂志 2017年第5期17卷 841-845页

作者：姚雪雁杨薇李海波许惠卓中南大学湘雅医院眼科中国湖南省长沙市410008

目的:探索构建稳定表达IL-33的原代大鼠骨髓间充质干细胞(BMSC)细胞系的方法。方法:体外分离、培养、鉴定大鼠骨髓间充质干细胞,并进行ELISA实验检测转染前IL-33的表达情况。构建GV303 Rat IL-33慢病毒载体,并进行慢病毒的包装。3d后荧... 详细信息

目的:探索构建稳定表达IL-33的原代大鼠骨髓间充质干细胞(BMSC)细胞系的方法。方法:体外分离、培养、鉴定大鼠骨髓间充质干细胞,并进行ELISA实验检测转染前IL-33的表达情况。构建GV303 Rat IL-33慢病毒载体,并进行慢病毒的包装。3d后荧光显微镜下观察GFP的表达,分别使用Western blot、ELISA检测转染后的BMSC中IL-33的表达。结果:大鼠BMSC被成功分离并传代,流式细胞术鉴定BMSC可得到CD90、CD29为阳性,CD11b、CD45、CD34为阴性,与之前的文献报道相符。并且BMSC被成功诱导分化为骨细胞、软骨细胞、脂肪细胞。对转染前的BMSC行ELISA实验检测IL-33的表达,结果为阴性。病毒转染3d后于荧光显微镜下可观察到GFP的表达;ELISA实验检测转染后BMSC中IL-33的表达,结果为阳性;Western blot亦可以检测到IL-33的表达。结论:应用GV303慢病毒载体可以建立稳定表达IL-33的大鼠BMSC原代细胞系。

关键词： IL-33 转染骨髓间充质干细胞实验性自动免疫性葡萄膜炎

EZH2-dependent myelination following sciatic nerve injury

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Neural Regeneration Research 2025年第8期20卷 2382-2394页

作者： hui Zhu Li Mu Xi xu Tianyi Huang Ying Wang Siyuan xu Yiting Wang Wencong Wang Zhiping Wang Hongkui Wang Chengbin xue Research Center of Clinical Medicine Affiliated Hospital of Nantong UniversityKey Laboratory of Neuroregeneration of Jiangsu and Ministry of EducationCo-innovation Center of NeuroregenerationNMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology ProductsNantong UniversityNantongJiangsu ProvinceChina Medical School of Nantong University NantongJiangsu ProvinceChina Department of Neurology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Rehabilitation Medicine Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Department of Critical Care Medicine Nantong Fourth People’s HospitalNantongJiangsu ProvinceChina

Demyelination and remyelination have been major focal points in the study of peripheral nerve regeneration following peripheral nerve ***,the gene regulatory network of regenerated myelin differs from that of native *** of enhancer of zeste homolog 2(EZH2)hinders the differentiation,maturation,and myelination of Schwann cells in *** further determine the role of EZH2 in myelination and recovery post-peripheral nerve injury,conditional knockout mice lacking Ezh2 in Schwann cells(Ezh2^(fl/fl);Dhh-Cre and Ezh2^(fl/fl);Mpz-Cre)were *** results show that a significant proportion of axons in the sciatic nerve of Ezh2-depleted mice remain *** highlights the crucial role of Ezh2 in initiating Schwann cell ***,we observed that 21 days after inducing a sciatic nerve crush injury in these mice,most axons had remyelinated at the injury site in the control nerve,while Ezh2^(fl/fl);Mpz-Cre mice had significantly fewer remyelinated axons compared with their wild-type *** suggests that the absence of Ezh2 in Schwann cells impairs myelin formation and *** conclusion,EZH2 has emerged as a pivotal regulatory factor in the process of demyelination and myelin regeneration following peripheral nerve *** EZH2 activity during these processes may offer a promising therapeutic target for the treatment of peripheral nerve injuries.

关键词： demyelination EZH2 myelination peripheral nerve injury PRC2 remyelination Schwann cells sciatic nerve crush sciatic nerve transection

高糖对视网膜Müller细胞VEGF,EPO和EPOR mRNA表达的影响

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国际眼科杂志 2010年第3期10卷 449-452页

作者：郭龙许惠卓夏晓波毛俊峰中南大学湘雅医学院中国湖南省长沙市410008 中南大学湘雅医院眼科中国湖南省长沙市410008

目的:观察高糖条件下VEGF mRNA,EPO mRNA和EPORmRNA在体外培养的Mller细胞中的表达情况。方法:胰蛋白酶将新生小鼠视网膜组织吹打消化后,制成单细胞悬液,体外培养Mller细胞,RT-PCR测定高糖条件下视网膜Mller细胞VEGF,EPO和EPOR基... 详细信息

目的:观察高糖条件下VEGF mRNA,EPO mRNA和EPORmRNA在体外培养的Mller细胞中的表达情况。方法:胰蛋白酶将新生小鼠视网膜组织吹打消化后,制成单细胞悬液,体外培养Mller细胞,RT-PCR测定高糖条件下视网膜Mller细胞VEGF,EPO和EPOR基因的表达。结果:成功获得视网膜Mller细胞,传代后90%以上的细胞呈兔抗鼠谷氨酰胺合酶(GS)染色阳性。Mller细胞VEGF mRNA,EPO mRNA和EPOR mRNA在高糖条件下表达升高,且呈浓度依赖性(P<0.05),但糖浓度50mmol/L组较40mmol/L组差异无统计学意义,无明显时间依赖性。结论:Mller细胞在高糖条件下VEGF,EPO,EPOR的表达增加。

关键词：视网膜Mller细胞血管内皮生长因子促红细胞生成素促红细胞生成素受体新生血管