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The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing zhang Xue Fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

TGF-β1对大鼠肾小球系膜细胞ⅩⅧ型胶原表达的影响

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复旦学报（医学版） 2004年第6期31卷 571-574,F006页

作者：杨静蒋涛李慧赵仲华张秀荣张农复旦大学上海医学院病理学系上海200032

目的　探讨转化生长因子 β1(TGF β1)对ⅩⅧ型胶原在大鼠肾小球系膜细胞中表达的影响 ,以及两者在大鼠抗Thy 1肾小球肾炎中的相互关系。方法　采用RT PCR、Westernblot法观察外源性TGF β1对大鼠肾小球系膜细胞中ⅩⅧ型胶原表达的影响 ... 详细信息

目的　探讨转化生长因子 β1(TGF β1)对ⅩⅧ型胶原在大鼠肾小球系膜细胞中表达的影响 ,以及两者在大鼠抗Thy 1肾小球肾炎中的相互关系。方法　采用RT PCR、Westernblot法观察外源性TGF β1对大鼠肾小球系膜细胞中ⅩⅧ型胶原表达的影响 ;制备大鼠抗Thy 1肾小球肾炎模型 ,应用免疫组织化学ABC方法分别观察TGF β1和ⅩⅧ型胶原在大鼠肾组织中的表达 ,并对其染色强度作图像定量分析。结果　外源性TGF β1作用后 ,大鼠肾小球系膜细胞中ⅩⅧ型胶原的表达升高 ,并显示出一定的时间与浓度依赖性。大鼠抗Thy 1肾小球肾炎中TGF β1的表达在 1、3和 5d仅有微量表达 ,第 7天开始其表达明显且随时间增强 ;ⅩⅧ型胶原和Ⅳ型胶原的表达趋势都与TGF β1吻合 ,ⅩⅧ型胶原的表达与TGF β1的表达间具有显著的相关性。结论　ⅩⅧ型胶原的表达与TGF β1的作用相关 ,可能参与了肾小球肾炎的发生发展过程。

关键词：外源性TGF-β1 大鼠胶原表达肾小球系膜细胞胶原肾小球肾炎观察 RT-PCR blot法转化生长因子-β1

分泌型抗肝癌单链双功能抗体融合GFP真核表达载体的构建及表达

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世界华人消化杂志 2001年第6期9卷 640-644页

作者：程虹刘彦仿张惠中沈万安张素珍中国人民解放军第四军医大学基础部病理学教研室陕西省西安市710033 中国人民解放军第四军医大学唐都医院骨科陕西省西安市710038

目的分泌型抗肝癌单链双功能抗体融合GFP真核表达载体的构建及表达。方法采用PCR方法在抗肝癌sFv的5端引入引导序列使其能够在真核细胞中表达并分泌,在其下游连接人TNF-α基因,构建分泌型抗肝癌单链双功能抗体基因,并将该基因克隆入带有... 详细信息

目的分泌型抗肝癌单链双功能抗体融合GFP真核表达载体的构建及表达。方法采用PCR方法在抗肝癌sFv的5端引入引导序列使其能够在真核细胞中表达并分泌,在其下游连接人TNF-α基因,构建分泌型抗肝癌单链双功能抗体基因,并将该基因克隆入带有GFP报告基因的真核表达载体,用磷酸钙共沉淀法转染小鼠成纤维细胞NIH3T3进行瞬时表达。结果 DNA序列分析证实在sFv的5端引入正确的60bp引导肽序列,酶切鉴定证明成功构建了分泌型抗肝癌单链双功能抗体融合GFP真核表达载体,并在NIH3T3细胞中成功地表达了融合荧光蛋白。结论成功构建并表达了分泌型抗肝癌单链双功能抗体融合GFP基因,为肝癌免疫基因治疗的进一步研究奠定了基础。

关键词：肝细胞癌单链抗体肿瘤坏死因子绿色荧光蛋白基因表达

An Efficient Boron Source Activation Strategy for the Low‑Temperature Synthesis of Boron Nitride Nanotubes

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Nano-Micro Letters 2025年第1期17卷 548-558页

作者： Ying Wang Kai zhang Liping Ding Liyun Wu Songfeng E Qian He Nanyang Wang hui Zuo Zhengyang Zhou Feng Ding Yue Hu Jin zhang Yagang Yao National Laboratory of Solid State Microstructures College of Engineering and Applied SciencesJiangsu Key Laboratory of Artificial Functional Materialsand Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093People’s Republic of China School of Electronic Information and Artificial Intelligence Shaanxi University of Science&TechnologyXian 710000People’s Republic of China College of Bioresources Chemical and Materials Engineering Shaanxi University of Science&TechnologyXian 710000People’s Republic of China Key Laboratory of Carbon Materials of Zhejiang Province College of Chemistry and Materials EngineeringWenzhou UniversityWenzhou 325000People’s Republic of China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518000People’s Republic of China College of Chemical and Molecular Engineering Peking UniversityBeijing 100871People’s Republic of China

Lowering the synthesis temperature of boron nitride nanotubes(BNNTs)is crucial for their *** primary reason for adopting a high temperature is to enable the effective activation of highmelting-point solid *** this study,we developed a novel approach for efficiently activating boron by introducing alkali metal compounds into the conventional MgO–B *** approach can be adopted to form various low-melting-point AM–Mg–B–O growth *** growth systems have improved catalytic capability and reactivity even under low-temperature conditions,facilitating the synthesis of BNNTs at temperatures as low as 850℃.In addition,molecular dynamics simulations based on density functional theory theoretically demonstrate that the systems maintain a liquid state at low temperatures and interact with N atoms to form BN *** findings offer novel insights into the design of boron activation and are expected to facilitate research on the low-temperature synthesis of BNNTs.

关键词： Boron nitride nanotubes Low-temperature Boron activation Density functional theory

基于空间相似关系的居民地群组目标地图综合质量评价

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地理与地理信息科学 2017年第2期33卷 52-56,68页

作者：刘涛段晓旗闫浩文王中辉张黎明兰州交通大学测绘与地理信息学院甘肃兰州730070 甘肃省地理国情监测工程实验室甘肃兰州730070

数字环境下的计算机自动制图综合质量评价,其本质就是计算综合前原图和综合后结果图之间的相似度,因此该文提出了基于空间相似关系对居民地群组地图要素的制图综合质量进行评价的方法。首先,根据多尺度空间相似关系的定义,提取出居民地... 详细信息

数字环境下的计算机自动制图综合质量评价,其本质就是计算综合前原图和综合后结果图之间的相似度,因此该文提出了基于空间相似关系对居民地群组地图要素的制图综合质量进行评价的方法。首先,根据多尺度空间相似关系的定义,提取出居民地群组多边形的特征因子并计算各因子综合前后的相似度;其次,在合理分配权重的基础上,建立居民地群组多边形总体相似度计算模型,并根据相似度计算结果对居民地群组目标要素的制图综合质量进行评价;最后,利用实验验证了该文所提评价方法的有效性。该研究为客观评价地图综合质量提供了一个新的思路。

关键词：空间相似关系地图综合质量评价居民地群

Homogeneous nanoparticle dispersion prepared with impurity-free dispersant by the ball mill technique

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Particuology 2013年第4期11卷 441-447页

作者： Lingyun Zhou hui zhang hui zhang zhong zhang National Center for Nanoscience and Technology Beijing 100190 China University of Chinese Academy of Sciences Beijing 100049 China

The homogeneous dispersion of nanoparticles in solvents or polymer matrices is essential for prac- tical application of nanocomposites. In this study, the planetary ball milling technique was used to de-agglomerate silica nanoparticles in butyl acetate. The size of the nanosilica aggregates was evalu- ated by TEM and SEM. With the addition of polyacrylate polymer to the organic solvent, the nanoparticle agglomerates were effectively broken up by planetary ball milling at the proper milling time; however, re-agglomeration occurred after a longer milling time. The results of TGA and FTIR indicated that the polyacrylate molecules could be adsorbed in situ onto the nanoparticles. Behaving similar to a dispersant, the adsorbed polyacrylate reduced the blend viscosity significantly and prevented re-agglomeration of the nanoparticles. Utilizing the polyacrylate polymer both as the dispersant and the polymer matrix, the polyacrylate-based nanocoatings were further prepared. The optical transmittance and haze value of the nanocoatings were found to be sensitive to the dispersion level of the nanoparticles, and the elastic mod- ulus and hardness of the nanocoatings were improved in comparison with those of the neat polymer coating.

关键词： NanoparticleNanocoatingBall millDispersant

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锅炉管式空气预热器的利用系数

节能技术 2001年第3期19卷 14-16页

作者：钟辉张军撒应禄东南大学

本文分析了锅炉管式空气预热器利用系数的真实含义 ,对不同的热力计算方法中的利用系数进行了正确比较。

关键词：锅炉管式空气预热器利用系数热力计算方法比较

染色体7q32-ter鼻咽癌相关基因的初步研究

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生物化学与生物物理进展 2001年第5期28卷 711-716页

作者：张小慧李忠花张必成董利周鸣曹利唐珂李伟芳李桂源中南大学湘雅医学院肿瘤研究所长沙410078

为克隆 7q32 ter区域等位基因杂合性丢失最小共同缺失区的鼻咽癌相关基因 .以STSD7S5 0 9为探针 ,PCR法筛选位于该区的细菌人工染色体 (BAC)克隆 ,应用EST介导的定位候选克隆策略并结合生物信息学筛选出在鼻咽癌细胞株和活检组织中表... 详细信息

为克隆 7q32 ter区域等位基因杂合性丢失最小共同缺失区的鼻咽癌相关基因 .以STSD7S5 0 9为探针 ,PCR法筛选位于该区的细菌人工染色体 (BAC)克隆 ,应用EST介导的定位候选克隆策略并结合生物信息学筛选出在鼻咽癌细胞株和活检组织中表达增强的ESTAA7734 5 4,cDNA克隆测序和生物信息学资源获取全长cDNA ,DNA印迹和甲基化分析研究其表达增强的机制 .结果表明 ,克隆的NAG18基因cDNA全长 80 2bp ,编码 2 2 7个氨基酸 ,定位于胞核 .该基因分别与人、鼠TAXREB10 7基因及RPL6基因高度同源 .其表达增强的机制不是基因拷贝数的丢失和甲基化位点的改变 .可以断定NAG18是定位于 7q32 ter最小共同缺失区的鼻咽癌相关基因 ,它是一高度保守的基因。

关键词： 7q32-ter最小共同缺失区鼻咽癌相关基因染色体

The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： Shengyang Zhou Ting Li Wei zhang Jian Wu hui Hong Wei Quan Xinyu Qiao Chun Cui Chenmeng Qiao Weijiang Zhao Yanqin Shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon