检索结果-南通市图书馆

Expression and purification of bioactive high-purity human midkine in Escherichia coli

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 79-86页

作者： zhong-hui zhang Li-juan DU Di XIANG Shun-ying ZHU Ming-yuan WU hui-li LU Yan YU Wei HAN Laboratory of Regenoromics School of Pharmacy College of Life Science and Biotechnology Shanghai Municipality Key Laboratory of Veterinary Biotechnology School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China

Midkine is a heparin-binding growth factor, which plays important roles in the regulation of cell growth and differentiation. The non-tagged recombinant human midkine （rhMK） is therefore required to facilitate its functional studies of this important growth factor. In the present work, rhMK was expressed in Escherichia coli （E. coli） BL21 （DE3）. The expression of midkine was efficiently induced by isopropyl-13-D-thiogalactopyranoside （IPTG）. After sonication, midkine was recovered in an insoluble form, and was dissolved in guanidine hydrochloride buffer. Renaturation of the denatured protein was carried out in the defined protein refolding buffer, and the refolded protein was purified using S-Sepharose ion-exchange chromatography. The final preparation of the rhMK was greater than 98% pure as measured by sodium dodecylsulfate-polyacrylamid gel electrophoresis （SDS-PAGE） and reverse phase high performance liquid chromatography （RP-HPLC）. The purified rhMK enhanced the proliferation of NIH3T3 cells.

关键词： Exnression. Purification. Human midkine. Escherichia coli

Electrodeposition of multi-layer Pd-Ni coatings on 316L stainless steel and their corrosion resistance in hot sulfuric acid solution

在线全文

学校读者我要写书评

暂无评论

Transactions of Nonferrous Metals Society of China 2017年第7期27卷 1543-1550页

作者： hui-zhong zhang Yang LI Yu ZUO Xu-hui ZHAO Yu-ming TANG Beijing Key Laboratory of Electrochemical Process and Technology for Materials Beijing University of Chemical Technology Beijing 100029 China

Pd-Ni coating shows good corrosion resistance in strong corrosion ***,in complex aggressiveenvironments,the performance of the coatings is limited and further improvement is *** effects of the applied platingcurrent density on the composition,structure and properties of Pd-Ni coatings were *** changing the current density in thesame bath,multi-layer Pd-Ni coatings were prepared on316L stainless *** electronic microscopy,weight loss tests,adhesion strength,porosity and electrochemical methods were used to study the corrosion resistance of the films prepared bydifferent coating *** with the single layer Pd-Ni coating,the multi-layer coatings showed higher microhardness,lower internal stress,lower porosity and higher adhesive *** multi-layer Pd-Ni coating showed obviously better corrosionresistance in hot sulfuric acid solution containing Cl-.

关键词： Pd-Ni film stainless steel multi-layer film electroplating current density corrosion resistance

Effects of sargentgloryvine stem extracts on HepG-2 cellsin vitro andin vivo

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2011年第23期17卷 2848-2854页

作者： Ming-Hua Wang Min Long Bao-Yi Zhu Shu-hui Yang Ji-Hong Ren hui-zhong zhang Department of LaboratoryMedicine and Research Center Tangdu Hospital Fourth MilitaryMedical University Xi＇an 710038 Shaanxi Province China

AIM:To observe the effects of sargentgloryvine stem extracts (SSE) on the hepatoma cell line HepG-2 in vitro andin vivo and determine its mechanisms of ***:Cultured HepG-2 cells treated with SSE were analysed by 3-(4,5-Dimethyl-thiazol-2-yl)-2,5Diphenyltetrazolium bromide and clone formation *** cell cycle and apoptosis analysis were conducted by flow cytometric,TdT-Mediated dUTP Nick End Labeling and acridine orange/ethidium bromide staining methods,and protein expression was examined by both reverse transcriptase-polymerase chain reaction and Western *** pathological changes of the tumor cells were observed by haematoxylin and eosin *** growth inhibition and side effects were determined in a xenograft mouse ***:SSE treatment could not only inhibit HepG-2 cell proliferation in a doseand time-dependent manner but also induce apoptosis and cell cycle arrest at the S *** number of colonies formed by SSEtreated tumor cells was fewer than that of the controls (P0.05).Systemic administration of SSE could inhibit the HepG-2 xenograft tumor growth with no obvious toxic side effects on normal ***:SSE can induce apoptosis of HepG-2 cells in vitro and in vivo through decreasing expression of Bcl-xl and Mcl-1 and increasing expression of Bax.

关键词： Sargentgloryvine stem extract Apoptosis Human hepatocellular carcinoma HepG-2 Bcl-2 family

Low prevalence of mismatch repair deficiency in Chinese colorectal cancers:a multicenter study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Gastroenterology Report 2020年第5期8卷 399-403,I0003页

作者： Wu Jiang Qiao-Qi Sui Wen-Liang Li Chuan-Feng Ke Yi-Hong Ling Le-En Liao Zhu Zhu Mu-Yan Cai Jun Luo Lin-Lin Mao hui-zhong zhang De-Sen Wan Zhi-zhong Pan Hai-Xing Ju Pei-Rong Ding Department of Colorectal Surgery Sun Yat-sen University Cancer CenterGuangzhouGuangdongP.R.China State Key Laboratory of Oncology in South China GuangzhouGuangdongP.R.China Collaborative Innovation Center for Cancer Medicine GuangzhouGuangdongP.R.China Department of Surgical Oncology First Affiliated Hospital of Kunming Medical UniversityKunmingYunnanP.R.China Department of Gastrointestinal Surgery The First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongP.R.China Department of Pathology Sun Yat-sen University Cancer CenterGuangzhouGuangdongP.R.China Department of Colorectal Surgery Zhejiang Cancer HospitalHangzhouZhejiangP.R.China Department of Clinical Genome Center Guangzhou KingMed Diagnostics Group Co.LtdGuangzhouGuangdongP.R.China

Background:Although universal testing for mismatch repair deficiency(dMMR)has been recommended to all colorectal cancer(CRC)patients,related evidence for the Chinese population is ***,we investigated the prevalence and clinicopathological features of dMMR patients in a large Chinese CRC ***:We included 7,373 CRC patients treated at four Chinese medical centers between August 2010 and September ***’baseline characteristics and pathological features were *** clinicopathological features were compared between patients with MLH1/PMS2 deficiency(dMLH1/PMS2)and MSH2/MSH6 deficiency(dMSH2/MSH6).Results:Among the investigated patients,654(8.9%)were identified with dMMR CRCs and,of them,401(61.3%)were males,with a median age of 55 years(range,22-87 years);355(54.3%)had stage II CRC based on American Joint Committee on Cancer 8th *** prevalence of the dMLH1/PMS2 group and the dMSH2/MSH6 group were 51.5%(337/654)and 25.1%(164/654),*** with dMSH2/MSH6 patients,those with dMLH1/PMS2 were older(57 vs 52 years,P<0.001),more likely to be female(45.7%vs 31.5%,P=0.004),prone to having tumors located in the right-hand side of the colon(59.0%vs 47.6%,P=0.015),and less likely to have a family history of tumors(29.7%vs 43.3%,P=0.003).Conclusions:The prevalence of dMMR in Chinese CRC patients was low,especially in the dMLH1/PMS2 *** clinicopathological features were different between dMMR subgroups.

关键词： prevalence mismatch repair deficiency colorectal cancer

Expression of G3BP and RhoC in esophageal squamous carcinoma and their effect on prognosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第30期13卷 4126-4130页

作者： hui-zhong zhang Jin-Geng Liu Yi-Ping Wei Cheng Wu Yong-Ke Cao Mei Wang Department of Cardiothoracic Surgery The Second Affiliated Hospital Sun Yat-Sen University 107 Yan- Jiang Road Guangzhou 510120 Guangdong Province China

AIM: To investigate the expression and significance of G3BP and RhoC proteins in esophageal squamous carcinoma (ESC). METHODS: The expression of G3BP and Rhoc proteins in 80 cases of ESC was detected by immunohistochemistry. The relationship was studied between the expression of the two proteins and tumor size, differentiation degree, TNM stage, lymph node metastasis and prognosis of ESC. RESULTS: The positive expression rate of G3BP in ESC was 71.25%; and the rate in the lymph node metastasis group was significantly higher than that in the non- lymph node metastasis group (Z = -2.283, P = 0.022), but no relations were found between G3BP expression and tumor size, differentiation degree and TNM stage (P > 0.05). The group with G3BP positive expression had shorter survival time than the group with G3BP negative expression (P = 0.000). The positive expression rate of RhoC in ESC was 66.25%; and the rate in the lymph node metastasis group was significantly higher than that in the non-lymph node metastasis group (Z = -2.115, P 0.05). The RhoC positive expression group had a shorter survival time than the RhoC negative expression group (P < 0.001. The expression of G3BP protein correlated positively with the expression of RhoC in ESC tissues (rs = 0.656, P < 0.001). CONCLUSION: The expression of G3BP and RhoC protein is closely related to the lymph node metastasis and survival in ESC patients. G3BP and RhoC proteins can be considered as predictors of prognosis in ESC patients.

关键词： Esophageal neoplasm G3BP RhoC Immunohistochemistry Lymph node metastasis Prognosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

浅埋煤层开采顶板切落条件下支架动载效应

煤炭学报 2017年第2期42卷 322-327页

作者：陈忠辉张凌凡杨登峰江博文中国矿业大学(北京)力学与建筑工程学院北京100083

针对浅埋煤层开采周期来压过程中顶板沿煤壁切落时对支架造成冲击的现象,根据基本顶回转切落造成直接顶岩体非线性破坏特征,建立了由基本顶-直接顶-支架-采空区矸石组成的力学模型,应用突变理论和岩石动力学方法,推导了直接顶失稳前后... 详细信息

针对浅埋煤层开采周期来压过程中顶板沿煤壁切落时对支架造成冲击的现象,根据基本顶回转切落造成直接顶岩体非线性破坏特征,建立了由基本顶-直接顶-支架-采空区矸石组成的力学模型,应用突变理论和岩石动力学方法,推导了直接顶失稳前后基本顶切落荷载作用下的支架压缩量表达式,分析了顶板切落过程中的动载效应,获得了顶板切落条件下支架工作阻力。研究结果表明:基本顶回转切落和直接顶失稳破坏是造成支架冲击的主因,直接顶岩体变形的突变量决定了冲击荷载的大小,系统失稳时的支架荷载除了与基本顶的周期来压步距、上覆岩层荷载、直接顶厚度有关外,还与支架和直接顶岩体的刚度比、直接顶岩体性质等因素相关。

关键词：浅埋煤层顶板切落突变理论动载效应

在线全文

学校读者我要写书评

暂无评论

Connecting the World

China Today 2025年第1期74卷 28-31页

作者： zhang hui 不详

Over 200 international exhibitors flock to the second China International Supply Chain Expo,showing the global market’s faith in the Chinese *** presence of over 200 international exhibitors at the second China International Supply Chain Expo(CISCE)in Beijing demonstrated what the global market thinks of the world’s second largest economy.

关键词： Supply economy. CISC

Rational modulation of fluorophosphate cathode by anionic groups to reduce the polarization behavior for fast-charging sodium-ion batteries

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Energy Chemistry 2025年第1期100卷 509-521页

作者： Xinyuan Wang Fan zhang Xingyu Zhou Qian Wang Changyu Liu Yangyang Liu hui Wang Xiaojie Liu Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education College of Chemistry & Materials Science Northwest University

Na3V2（PO4）2O2F （VP） is recognized as a promising cathode material for sodium-ion batteries due to its stable structural framework and high specific *** functional theory （DFT） and finite element simulations show that incorporating SO42-into VP decreases its band gap,lowers the migration energy barrier,and ensures a uniform Na+concentration gradient and stress distribution during charge and discharge ***,the average Na+diffusion coefficient of Na3V2（PO4）1.95（SO4）0.05O2F（VPS-1） is roughly double that of VP,leading to enhanced rate capability (80 C,75.5 mAh g-1) and cycling stability (111.0 mAh g-1capacity after 1000 cycles at 10 C current density) for ***-1 exhibits outstanding fast-charging capabilities,achieving an 80%state of charge in just 8.1 *** assembled VPS-1//SbSn/NPC full cell demonstrated stable cycling over 200 cycles at a high 5 C current,maintaining an average coulombic efficiency of 95.35%.

关键词： Anionic group modulation Polarization behavior Fast-charging Sodium-ion battery Fluorophosphate Cathode

circ_0000212靶向miR-139-5p对肝癌细胞增殖、迁移、侵袭、凋亡以及紫杉醇敏感性的影响

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

世界华人消化杂志 2021年第22期29卷 1276-1285页

作者：张惠忠张晓东黄建新金华广福肿瘤医院肝胆胰胃外科浙江省金华市321111

背景circ_0000212是一种新发现的非编码RNA,其高表达可促进结直肠癌进展.然而,circ_0000212在肝癌中的表达模式和作用仍然未知.目的探讨circ_0000212靶向miR-139-5p对肝癌细胞增殖、迁移、侵袭、凋亡以及紫杉醇敏感性的影响.方法RT-qPC... 详细信息

背景circ_0000212是一种新发现的非编码RNA,其高表达可促进结直肠癌进展.然而,circ_0000212在肝癌中的表达模式和作用仍然未知.目的探讨circ_0000212靶向miR-139-5p对肝癌细胞增殖、迁移、侵袭、凋亡以及紫杉醇敏感性的影响.方法RT-qPCR检测circ_0000212和miR-139-5p在肝癌组织、癌旁组织中的表达.Pearson相关分析确定肝癌组织中circ_0000212和miR-139-5p表达的关系.双荧光素酶报告实验验证circ_0000212和miR-139-5p靶向关系.将肝癌细胞HCC9204分为对照组、干扰circ_0000212组、干扰circ_0000212+miR-139-5p抑制物组、紫杉醇组、紫杉醇+干扰circ_0000212组、紫杉醇+干扰circ_0000212+miR-139-5p抑制物组.CCK-8法检测HCC9204细胞抑制率;集落形成实验检测HCC9204细胞集落形成数;流式细胞术检测HCC9204细胞凋亡率;Transwell实验检测HCC9204细胞迁移和侵袭数.结果与癌旁组织比较,肝癌组织中circ_0000212表达水平显著升高(P<0.05),miR-139-5p表达水平显著降低(P<0.05).肝癌组织中circ_0000212和miR-139-5p表达呈负相关关系.circ_0000212和miR-139-5p存在直接相互作用.与对照组比较,紫杉醇组HCC9204细胞circ_0000212表达水平显著降低(P<0.05),miR-139-5p表达水平显著升高(P<0.05).与对照组比较,干扰circ_0000212组、紫杉醇组HCC9204细胞克隆形成数、迁移数、侵袭数显著降低(P<0.05),抑制率、凋亡率显著升高(P<0.05).与干扰circ_0000212组比较,干扰circ_0000212+miR-139-5p抑制物组HCC9204细胞克隆形成数、迁移数、侵袭数显著升高(P<0.05),抑制率、凋亡率显著降低(P<0.05).与紫杉醇组比较,紫杉醇+干扰circ_0000212组HCC9204细胞克隆形成数、迁移数、侵袭数显著降低(P<0.05),抑制率、凋亡率显著升高(P<0.05).与紫杉醇+干扰circ_0000212组比较,紫杉醇+干扰circ_0000212+miR-139-5p抑制物组HCC9204细胞克隆形成数、迁移数、侵袭数显著升高(P<0.05),抑制率、凋亡率显著降低(P<0.05).结论干扰circ_0000212通过靶向上调miR-139-5p可抑制肝癌细胞增殖、迁移、侵袭能力,诱导细胞凋亡,并提高其对紫杉醇的敏感性.

关键词： circ_0000212 miR-139-5p 肝癌细胞增殖凋亡迁移侵袭紫杉醇敏感性