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Involvement of nuclear factor-kappa B on corticosteroneinduced rat Leydig cell apoptosis

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Asian Journal of Andrology 2006年第6期8卷 693-702页

作者： Qian wang hui-Bao gao Department of Biochemistry and Molecular Biology School of Medicine Shanghai Jiao Tong University Shanghai 200025 China

Aim： To investigate the activation of nuclear factor-kappa B （NF-kappa B） and its function in glucocorticoid-induced Leydig cell apoptosis. Methods： The Leydig cells were isolated from male Sprague-Dawley rats （90 days of age） and were incubated with corticosterone （CORT, glucocorticoid in rat） for 6 h, 12 h and 24 h, respectively. The P65 subunit of NF-kappa B （NF-kappa B/P65） in nuclei and the inhibitor of NF-kappa B （Ikappa B） in cytoplasm were analyzed by Western-blotting. The Leydig cells were treated with anti-Fas antibody for 3 h followed by Western blotting to assay the changes of NF-kappa B/P65 in nuclei and in cytoplasm. The role of NF-kappa B in CORT- induced Leydig cell apoptosis was evaluated by observing the effects of NF-kappa B/P65 overexpression and inhibiting activation of NF-kappa B by 100 μmol/L Pyrrolidine dithiocarbamate （PDTC） on this apoptosis. Results： The treatment of Leydig cells with CORT increased the levels of NF-kappa B/P65 in nuclei and decreased the levels of Ikappa B in cytoplasm. Following the Leydig cells were treated with anti-Fas antibody, the levels of NF-kappaB/P65 was increased in nuclei and decreased in cytoplasm. The CORT-induced Leydig cell apoptosis was inhibited by overexpressed NF-kappaB/P65 and was enhanced by incubation with PDTC. Conclusion： NF-kappa B is activated by increased FasL/Fas in CORT-induced Leydig cell apoptosis. NF-kappa B may play an anti-apoptotic role in this apoptosis.

关键词： rat Leydig cell NF-kappa B apoptosis corticosterone

Transurethral seminal vesiculoscopy in the diagnosis and treatment of persistent or recurrent hemospermia： a single- institution experience

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Asian Journal of Andrology 2009年第5期11卷 566-570,I0002页

作者： Zhi-Yong Liu Ying-Hao Sun Chuan-Liang Xu Jian-Guo Hou Xu gao Xin Lu Hua-Mao Ye Lin-hui wang Xiao-Feng gao Shanghai Changhai Hospital the Second Military Medical University Shanghai 200433 China

The diagnosis and treatment of hemospermia presents significant difficulty, especially if it persists or recurs. In this retrospective study, we assessed whether transurethral seminal vesiculoscopy is feasible and effective in the diagnosis and treatment of hemospermia. To address this complex condition, we report our experience in a population of patients treated with transurethral seminal vesiculoscopy. From February 2006 to July 2008, 72 hemospermic patients underwent transurethral seminal vesiculoscopy examination and treatment at our urology center. Transurethral seminal vesiculoscopy was performed by a 7-F or 8-F rigid ureteroscope. The endoscopic procedure was conducted through the normal anatomic route of the seminal tracts. In this series, the mean follow-up period was 21.7 months. Definite diagnosis was made for 93.1% patients, and 94.4% patients were cured or showed a decrease in their symptoms. Postoperative complications were not observed in the study. Our study proves that transurethral seminal vesiculoscopy is effective in the diagnosis and treatment of hemospermia with minimal complication.

关键词： diagnosis hemospermia transurethral seminal vesiculoscopy treatment

NFAT2 is implicated in corticosterone-induced rat Leydig cell apoptosis

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Asian Journal of Andrology 2007年第5期9卷 623-633页

作者： Wei-Ran Chai Qian wang hui-Bao gao Department of Biochemistry and Molecular Biology School of Medicine Shanghai Jiao Tong University Shanghai 200025China

Aim： To investigate the activation of the nuclear factor of activated T cells （NFAT） and its function in the corticosterone （CORT）-induced apoptosis of rat Leydig cells. Methods： NFAT in rat Leydig cells was detected by Western blotting and immunohistochemical staining. Cyclosporin A （CsA） was used to evaluate potential involvement of NFAT in the CORT-induced apoptosis of Leydig cells. Intracellular Ca^2＋ was monitored in CORT-treated Leydig cells using Fluo-3/AM. After the Leydig cells were incubated with either CORT or CORT plus CsA for 12 h, the levels of NFAT2 in the nuclei and in the cytoplasm were measured by semi-quantitative Western blotting. The role of NFAT2 in CORT- induced Leydig cell apoptosis was further evaluated by observing the effects of NFAT2 overexpression and the inhibition of NFAT2 activation by CsA on FasL expression and apoptosis. Results： We found that NFAT2 was the predominant isoform in Leydig cells. CsA blocked the CORT-induced apoptosis of the Leydig cells. The intracellular Ca^2＋ level in the Leydig cells was significantly increased after the CORT treatment. The CORT increased the level of NFAT2 in the nuclei and decreased its level in the cytoplasm. CsA blocked the CORT-induced nuclear translocation of NFAT2 in the Leydig cells. Both CORT-induced apoptosis and FasL expression in the rat Leydig cells were enhanced by the overexpression of NFAT2 and antagonized by CsA. Conclusion： NFAT2 was activated in CORT-induced Leydig cell apoptosis. The effects of NFAT2 overexpression and the inhibition of NFAT2 activation suggest that NFAT2 may potentially play a pro-apoptotic role in CORT-induced Leydig cell apoptosis through the up-regulation of FasL.

关键词： nuclear factor of activated T cells corticosterone Leydig cell apoptosis

Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future

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Neural Regeneration Research 2025年第4期20卷 946-959页

作者： Zhiyuan Yin Jiahui Kang Xuan Cheng hui gao Shujia Huo Haiwei Xu Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Southwest Eye HospitalSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased *** lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored ***,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various *** in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in ***,there remain issues that hinder improving reprogramming efficiency and ***,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming *** review describes recent progress in relatively successful Müller glia reprogramming *** also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关键词： cell fusion chemical small-molecules epigenetic extracellular matrix immune metabolic mice Müller glia neurodegenerative diseases reprogramming retina regeneration

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白矮主序双星光谱的参数自动测量研究

光谱学与光谱分析 2017年第9期37卷 2914-2918页

作者：姜斌刘淑慧王文玉高军山东大学(威海)机电与信息工程学院山东威海264209

白矮主序双星是一类致密的双星系统,主星是一颗白矮星,伴星是一颗小质量的M型主序星。白矮主序双星光谱的数量相对较少,但对该类天体的研究对于进一步理解密近双星的演化,特别是公共包层演化的物理机制等重要的天体物理热点有积极的意义... 详细信息

白矮主序双星是一类致密的双星系统,主星是一颗白矮星,伴星是一颗小质量的M型主序星。白矮主序双星光谱的数量相对较少,但对该类天体的研究对于进一步理解密近双星的演化,特别是公共包层演化的物理机制等重要的天体物理热点有积极的意义。SDSS-DR12是美国SLOAN巡天望远镜发布的最新数据,基于前期实验在其中发现的4,140个白矮主序双星光谱,通过最小二乘法对这些光谱进行分解后,使用模板匹配方法,测量了这批样本中两个子星的基本参数,包括有效温度、重力加速度、金属丰度等,并对结果进行了分析和统计,进一步丰富了白矮主序双星模板库。白矮主序双星的参数测量的主要问题是计算量大,在实验中使用了GPU技术,提高了匹配效率,在海量光谱处理方向进行了有益尝试。实验结果表明该方法对大型巡天望远镜的海量光谱参数自动处理有较重要的应用价值。

关键词：白矮主序双星参数测量模板匹配

基于动态博弈模型的付费搜索拍卖广义第二价格机制——规则、均衡与效率分析

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上海交通大学学报 2010年第3期44卷 349-353,359页

作者：姜晖王浣尘高朝伟上海交通大学安泰经济与管理学院上海200052

针对付费搜索拍卖问题,通过分析VCG(Vickrey-Clarke-Groves)机制优劣势以及搜索引擎结果网页(SERP)位置价值结构,引出并界定了作为简化VCG机制的广义第二价格(GSP)机制,证明了在广告主估价具有可分离的形式时,按点击付费并进行有效排名... 详细信息

针对付费搜索拍卖问题,通过分析VCG(Vickrey-Clarke-Groves)机制优劣势以及搜索引擎结果网页(SERP)位置价值结构,引出并界定了作为简化VCG机制的广义第二价格(GSP)机制,证明了在广告主估价具有可分离的形式时,按点击付费并进行有效排名的GSP机制能够实现VCG均衡.通过数值实验,讨论了动态环境下排名规则对均衡效率和搜索引擎收入的影响,指出了有效排名相对于单纯排名的优势.

关键词：付费搜索广义第二价格 VCG机制拍卖

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一种关联规则挖掘的优化算法

计算机工程与应用 2003年第26期39卷 204-207页

作者：王晖高飞深圳大学信息工程学院深圳518060

文中基于chi-square检验、有趣度及信息增益理论,给出了一种挖掘优化关联规则的算法。该算法将冗余的规则分为两个部分:一部分规则缺乏统计的相关性,而另外一部分规则不满足“新奇的”要求。实验结果表明算法可以有效地去除冗余规则并... 详细信息

文中基于chi-square检验、有趣度及信息增益理论,给出了一种挖掘优化关联规则的算法。该算法将冗余的规则分为两个部分:一部分规则缺乏统计的相关性,而另外一部分规则不满足“新奇的”要求。实验结果表明算法可以有效地去除冗余规则并提高挖掘效率。

关键词：关联规则挖掘有趣度信息增益

Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： Wen gao Jing-Wen Li hui Ye Xue-Zhi Zhang Jian-Xiang Liu Hong Cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

Inguinal Incision as an Successful Route To Extract the Kidney During Laparoscopic Retroperitoneal Live-donor Nephrectomy

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Inguinal Incision as an Successful Route To Extract the Kidn...