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Suppression of matrix metalloproteinase-2 via RNA interference inhibits pancreatic carcinoma cell invasiveness and adhesion

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World Journal of Gastroenterology 2009年第9期15卷 1072-1078页

作者： ying-hui Zhi Mao-min Song Pi-Lin Wang Tie Zhang Zi-yi yin Department of General Surgery Affiliated Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

AIM:To investigate the inhibitory effects of RNA interference (RNAi) on expression of matrix metalloproteinase-2 (MMP-2) gene and invasiveness and adhesion of human pancreatic cancer cell line,***:RNAi was performed using the vector (pGPU6)-based small interference RNA (siRNA) plasmid gene silence system to specifically knock down MMP-2 expression in pancreatic cancer cell line,BxPC-3. Four groups of different specific target sequence in coding region of MMP-2 and one non-specific sequence were chosen to construct four experimental siRNA plasmids of pGPU6-1,pGPU6-2,pGPU6-3 and pGPU6-4,and one negative control siRNA plasmid of pGPU6 (-). MMP-2 expression was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell proliferation and apoptosis were examined by methyl thiazolyl tetrazolium (MTT) and flow cytometry,respectively. The abilities of adhesion and invasion were detected by cell adhesion assay and cell invasion assay using Transwell ***:The expression of MMP-2 was inhibited and the inhibitory effects of different sequence varied. pGPU6-1 group had the most efficient inhibitory effect,followed by pGPU6-2 and pGPU6-3 *** and adhesion were more significantly reduced in pGPU6-1,pGPU6-2 and pGPU6-3 groups as compared with pGPU6 (-) and blank control groups. However,no difference concerning cell proliferation and apoptosis was observed after transfection between experiment groups and control ***:RNAi against MMP-2 successfully inhibited the mRNA and protein expression of MMP-2 in the pancreatic cancer cell line,BxPC-3,leading to a potent suppression of tumor cell adhesion and invasion without affecting cell proliferation and apoptosis. These findings suggest that the RNAi approach towards MMP-2 may be an effective therapeutic strategy for the clinical management of pancreatic tumor.

关键词： Pancreatic neoplasm Tumor metastasis Matrix metalloproteinase-2 Small interfering RNA Tumor invasiveness

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Midazolam in rabbits terminates dysrhythmias caused by intracerebroventricular ropivacaine

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第8期12卷 668-676页

作者： Yao-min ZHU Zu-yi YUAN hui WU Dan-dan ZHOU Gui-xia JING Department of Anesthesiology the First Affiliated Hospital School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China Department of Cardiology the First Affiliated Hospital School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China

The current study was designed to investigate the mechanisms by which ropivacaine may act within the central nervous system （CNS） to produce cardiotoxicity. Eighty New Zealand rabbits were divided into four groups randomly. In Group 1,20 rabbits received intracerebroventricular （icv） saline, and then received icv ropivacaine 30 min later. In Group 2, 20 rabbits received icv ropivacaine. Whenever dysrhythmias continued for more than 5 min, 0.1 ml saline was administered into the left cerebral ventricle. Ten minutes later, 0.1 ml midazolam was given into the left lateral ventricle. In Group 3, 20 rabbits received icv ropivacaine, and once the dysrhythmias developed, the inspired isoflurane concentration was increased from 0.75% to 1.50%. In Group 4, 20 animals received an intravenous （iv） phenylephrine infusion until dysrhythmias occurred. In Group 1, the rabbits did not develop dysrhythmias in response to icv saline, whereas dysrhythmias did develop in these animals after icv ropivacaine. In Group 2, icv saline had no effect on the dysrhythmias; however, icv midazolam terminated cardiac dysrhythmias. In Group 3, an increase in the concentration of the inspired isoflurane had no effect on dysrhythmias. In Group 4, icv midazolam had no effect on dysrhythmias in response to iv phenylephdne. Ropivacaine administered directly into the CNS is capable of producing cardiac dysrhythmias; midazolam terminated dysrhythmias presumably by potentiation of y-aminobutyric acid （GABA） receptor activity. Our results suggest that ropivacaine produces some of its cardiotoxicity not only by the direct cardiotoxicity of the drug, but also by the CNS effects of ropivacaine.

关键词： Ropivacaine Cardiotoxicity Ventricular cerebrospinal fluid Central nervous system (CNS) Dysrhythmias Midazolam

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Structure Model of Granules for Sintering Mixtures

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Journal of Iron and Steel Research International 2014年第10期21卷 905-909页

作者： Li-shun YUAN Xiao-hui FAN min GAN Gui-ming YANG yi WANG School of minerals Processing and Bioengineering Central South University

Structure model of granules, boundary value of nucleus and powder, and the relationship between granula- tion efficiency and boundary value were investigated. Granules of sintering mixtures are composed of adhesive powder and nucleus. In the mixtures, particles larger than 1.00 mm act as nucleus and particles smaller than 0.25 mm act as adhesive powder. Particles with size between 0.25--1.00 mm can be adhesive powder as well as nucleus depending on the granulation conditions. When the boundary value is close to 0.25 mm, the granulation efficiency is lower than 50%. When the boundary value is close to 1.00 mm, the granulation efficiency is above 90%. The boundary value is influenced by the iron ore type, granulation moisture, fineness of raw materials and burnt-lime activity. Good adhe- sive capability, suitable moisture content, appropriate particle size distribution and high burnt-lime activity make the boundary value move towards 1.00 mm and improve the granulation efficiency.

关键词： iron ore sintering structure model granulation

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Molecular Dynamics Study of Tension Process of Ni-Based Superalloy

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Acta Metallurgica Sinica(English Letters) 2020年第5期33卷 741-750页

作者： hui Li Wan Du yi Liu Materials Genome Institute Shanghai UniversityShanghai 200444China

To understand the atomistic mechanisms of tension failure of Ni-based superalloy,in this study,the classical molecular dynamics(MD)simulations were used to study the uniaxial tension processes of both the Ni/Ni3 Al interface systems and the pure Ni and Ni3 Al *** examine the effects of interatomic potentials,we adopted embedded atom method(EAM)and reactive force field(ReaxFF)in the MD *** results of EAM simulations showed that the amorphous structures and voids formed near the interface,facilitating further crack propagation within Ni *** EAM potentials also predicted that dislocations were generated and annihilated alternatively,leading to the oscillation of yielding stress during the tension *** ReaxFF simulations predicted more amorphous formation and larger tensile *** atomistic understanding of the defect initiation and propagation during tension process may help to develop the strengthening strategy for controlling the defect evolution under loading.

关键词： Ni-based superalloy Molecular dynamics Embedded atom method(EAM) Reactive force field(ReaxFF) Uniaxial tension

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Different damage patterns of retinal nerve fiber layer and ganglion cell-inner plexiform layer between early glaucoma and non-glaucomatous optic neuropathy

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International Journal of Ophthalmology(English edition) 2020年第6期13卷 893-901页

作者： hui Xiao Xing Liu Ping Lian Ling-Ling Liao yi-min Zhong State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060Guangdong ProvinceChina

AIM:To compare the damage pattern of the peripapillary retinal nerve fiber layer(pRNFL)and the macular ganglion cell-inner plexiform layer(mGCIPL)between early glaucomatous and non-glaucomatous optic neuropathy(EGON and NGON).METHODS:It is a cross-sectional ***-eight healthy controls,74 EGONs and 70 NGONs with comparable average pRNFL loss were *** NGON group included 23 eyes of optic neuritis(ON),13 eyes of hereditary optic neuropathy(HON),19 eyes of toxic optic neuropathy(TON)and 15 eyes of compressive neuropathy(CON).The sectoral pRNFL and mGCIPL thickness obtained by high definition optical coherence tomography were ***:Compared to normal controls,the pRNFL thickness in all quadrants showed a decrease in both EGON and NGON group(P0.05).The mGCIPL of EGON and NGON group were thinner than control group(P<0.001).In EGON group the severest sites of mGCIPL reduction was located at inferotemporal and inferior ***,compared to EGON group,the average mGCIPL of NGON groupwere significantly thinner,especially in superonasal and inferonasal sectors(P<0.001).CONCLUSION:The damage pattern of pRNFL and mGCIPL caused by glaucoma is distinct from other NGON such as ON,TON,HON and CON,and this characteristic damage pattern is helpful in differentiating early glaucoma from other NGON.

关键词： glaucoma optic neuropathy retinal nerve fiber layer ganglion cell-inner plexiform layer optical coherence tomography

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Application of lesser trochanteric reduction fixator in the treatment of unstable intertrochanteric fractures

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World Journal of Clinical Cases 2023年第25期11卷 5863-5869页

作者： Yao-min hui Guang Zeng Pei-yi Liu Bin Chai Department of Orthopedics Tong Ren HospitalSchool of Medicine Shanghai Jiao Tong UniversityShanghai 200336China

BACKGROUND Closed reduction and internal fixation with intramedullary nails has been widely accepted for treating intertrochanteric *** To focus on how to avoid displacement of the lesser trochanter in unstable intertrochanteric *** We developed a lesser trochanteric reduction fixator for treating intertrochanteric fractures through fixing the lesser trochanter by combining the loop plate through the fixator after reduction by the *** patients with intertrochanteric fractures treated with the newly developed lesser trochanteric reduction fixator and loop plate combined with intramedullary nails,and 20 patients with intertrochanteric fractures treated with simple intramedullary nails were selected from December 2020 to March *** The postoperative Harris hip score was significantly higher in patients treated with the lesser trochanteric reduction fixator than in patients treated without the lesser trochanteric reduction fixator,which indicated that this lesser trochanteric reduction fixator had a positive impact on rehabilitation of the hip joint after surgery and could significantly improve the quality of life of *** We fully realize the significance of trochanteric reduction and fixation,namely,reconstruction of structures under pressure,in the treatment of intertrochanteric *** long as the general condition of patients is favorable and they are willing to undergo surgery,fixation of the main fracture end should be performed and the lesser trochanter should be reduced and fixed at the same time.

关键词： Lesser trochanteric Intertrochanteric fractures Reduction fixator Loop plate Unstable fractures

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Multi-objective quality control method for cold-rolled products oriented to customized requirements

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International Journal of minerals,Metallurgy and Materials 2021年第8期28卷 1332-1342页

作者： yi-fan Yan Zhi-min Lü Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China

To deal with the increasing demand for low-volume customization of the mechanical properties of cold-rolled products, a two-way control method based on mechanical property prediction and process parameter optimization(PPO) has become an effective solution. Aiming at the multi-objective quality control problem of a company's cold-rolled products, based on industrial production data, we proposed a process parameter design and optimization method that combined multi-objective quality prediction and PPO. This method used the multi-output support vector regression(MSVR) method to simultaneously predict multiple quality indices. The MSVR prediction model was used as the effect verification model of the PPO results. It performed multi-process parameter collaborative design and realized the optimization of production process parameters for customized multi-objective quality requirements. The experimental results showed that, compared with the traditional single-objective quality prediction model based on support vector regression(SVR), the multi-objective prediction model could better take into account the coupling effect between process parameters and quality index, the MSVR model prediction accuracy was higher than that of the SVR, and the optimized process parameters were more capable and reflected the influence of metallurgical mechanism on the quality index,which were more in line with actual production process requirements.

关键词： customized production quality control multi-objective prediction multi-output support vector regression process parameter optimization

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Stable Label-Specific Features Generation for Multi-Label Learning via Mixture-Based Clustering Ensemble

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IEEE/CAA Journal of Automatica Sinica 2022年第7期9卷 1248-1261页

作者： yi-Bo Wang Jun-yi Hang min-Ling Zhang School of Computer Science and Engineering Southeast UniversityNanjing 210096China IEEE

Multi-label learning deals with objects associated with multiple class labels,and aims to induce a predictive model which can assign a set of relevant class labels for an unseen *** each class might possess its own characteristics,the strategy of extracting label-specific features has been widely employed to improve the discrimination process in multi-label learning,where the predictive model is induced based on tailored features specific to each class label instead of the identical instance *** a representative approach,LIFT generates label-specific features by conducting clustering ***,its performance may be degraded due to the inherent instability of the single clustering *** improve this,a novel multi-label learning approach named SENCE(stable label-Specific features gENeration for multi-label learning via mixture-based Clustering Ensemble)is proposed,which stabilizes the generation process of label-specific features via clustering ensemble ***,more stable clustering results are obtained by firstly augmenting the original instance repre-sentation with cluster assignments from base clusters and then fitting a mixture model via the expectation-maximization(EM)*** experiments on eighteen benchmark data sets show that SENCE performs better than LIFT and other well-established multi-label learning algorithms.

关键词： Clustering ensemble expectation-maximization al-gorithm label-specific features multi-label learning

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Phase-Field Simulation of Sintering Process:A Review

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Computer Modeling in Engineering & Sciences 2024年第8期140卷 1165-1204页

作者： ming Xue min yi State Key Laboratory ofMechanics and Control forAerospace Structures&Institute for Frontier Science&Key Laboratory for Intelligent Nano Materials and Devices of ministry of Education&College of Aerospace Engineering Nanjing University of Aeronautics and Astronautics(NUAA)Nanjing210016China

Sintering,a well-established technique in powder metallurgy,plays a critical role in the processing of high melting point materials.A comprehensive understanding of structural changes during the sintering process is essential for effective product *** phase-field method stands out for its unique ability to simulate these structural *** its widespread application,there is a notable absence of literature reviews focused on its usage in sintering ***,this paper addresses this gap by reviewing the latest advancements in phase-field sintering models,covering approaches based on energy,grand potential,and entropy *** characteristics of various models are extensively discussed,with a specific emphasis on energy-based models incorporating considerations such as interface energy anisotropy,tensor-form diffusion mechanisms,and various forms of rigid particle motion during ***,the paper offers a concise summary of phase-field sintering models that integrate with other physical fields,including stress/strain fields,viscous flow,temperature field,and external electric *** conclusion,the paper provides a succinct overview of the entire content and delineates potential avenues for future research.

关键词： Phase-field model review sintering additive manufacturing

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Synthesis and application of bilayer-surfactant-enveloped Fe_3O_4 nanoparticles: water-based bilayer-surfactant-enveloped ferrofluids

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International Journal of minerals,Metallurgy and Materials 2016年第2期23卷 234-240页

作者： Bai-yi Chen Jian-hui Qiu hui-xia Feng Department of Machine Intelligence and Systems Engineering Faculty of Systems Engineering Akita Prefectural University College of Petrochemical Technology Lanzhou University of Technology

Superparamagnetic carbon-coated Fe3O4 nanoparticles with high magnetization（85 emu·g-（-1）） and high crystallinity were synthesized using polyethylene glycol-4000（PEG（4000）） as a carbon ***3O4 water-based bilayer-surfactant-enveloped ferrofluids were subsequently prepared using sodium oleate and PEG（4000） as *** using X-ray photoelectron spectroscopy,X-ray diffraction,and Fourier-transform infrared spectroscopy indicate that the Fe3O4 nanoparticles with a bilayer surfactant coating retain the inverse spinel-type structure and are successfully coated with sodium oleate and PEG（4000）.Transmission electron microscopy,vibrating sample magnetometry,and particle-size analysis results indicate that the coated Fe3O4 nanoparticles also retain the good saturation magnetization of Fe3O4（79.6 emu·g^-1） and that the particle size of the bilayer-surfactant-enveloped Fe3O4 nanoparticles is 42.97 nm,which is substantially smaller than that of the unmodified Fe3O4 nanoparticles（486.2 nm）.UV-vis and zeta-potential analyses reveal that the ferrofluids does not agglomerate for 120 h at a concentration of 4 g·L^-1,which indicates that the ferrofluids are highly stable.

关键词： magnetic fluids iron oxides nanoparticles bilayers sodium oleate polyethylene glycol

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