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Petroleum Science 2022年第3期19卷 1236-1249页

作者： Qian Sang Xin-Yi Zhao hui-min liu ming-Zhe Dong Key Laboratory of Unconventional Oil&Gas Development(China University of Petroleum(East China)) Ministry of EducationQingdao266580ShandongChina School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Management Center of Oil&Gas Exploration of Shengli Oilfield Branch Company SINOPECDongying257001ShandongChina Department of Chemical and Petroleum Engineering University of CalgaryCalgaryABCanadaT2N 1N4

Shale oil formations contain both inorganic and organic *** organic matter holds both free oil in the pores and dissolved oil within the kerogen *** free oil flow in organic pores and the dissolved oil diffusion in kerogen molecules are coupled *** molecular flow of free n-alkanes is an important process of shale oil accumulation and *** study the dynamics of imbibition process of n-alkane molecules into kerogen slits,molecular dynamics(MD)simulations are *** of slit width,temperature,and n-alkane types on the penetration speed,dynamic contact angle,and molecular conformations were *** showed that molecular transportation of n-alkanes is dominated by molecular structure and molecular motion at this *** space-confinement conformational changes of molecules slow down the filling speeds in the narrow *** n-alkane molecules with long carbon chains require more time to undergo conformational *** high content of short-chain alkanes and high temperature facilitate the flow of alkane mixtures in kerogen *** obtained from this study are useful for understanding the underlying nanoscale flow mechanism in shale formations.

关键词： Molecular flow Imbibition n-Alkane Kerogen slit Molecular dynamics simulation

Ethanol induced mitochondria injury and permeability transition pore opening: Role of mitochondria in alcoholic liver disease

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World Journal of Gastroenterology 2007年第16期13卷 2352-2356页

作者： ming Yan Ping Zhu hui-min liu Hai-Tao Zhang Li liu Department of Digestive Diseases Qilu Hospital Shandong University Jinan 250012 Shandong Province China Jinan Infectious Disease Hospital Jinan 250012 ShandongProvince China

AIM: To observe changes of mitochondria and investigate the effect of ethanol on mitochondrial perme- ability transition pore (PTP), mitochondrial membrane potential (MMP, ΔΨm) and intracellular calcium concentration in hepatocytes by establishing an animal model of alcoholic liver disease (ALD). METHODS: Fourty adult male Wistar rats were randomly divided into two groups, the model group (20) was administered alcohol intragastrically plus an Oliver oil diet to establish an ALD model, and the control group (20) was given an equal amount of normal saline. The ultramicrostructural changes of mitochondria were observed under electron microscopy. Mitochondria of liver was extracted, and patency of PTP, mitochondrial membrane potential (ΔΨm), mitochondrial mass and intracellular calcium concentration of isolated hepacytes were detected by flow cytometry using rhodamine123 (Rh123), Nonyl-Acridine Orange and calcium fluorescent probe Fluo-3/AM, respectively. RESULTS: Membrane and cristae were broken or disappeared in mitochondria in different shapes under electron microscopy. Some mitochondria showed U shape or megamitochondrion. In the model group, liver mitochondria PTP was broken, and mitochondria swelled, the absorbance at 450 nm, A540 decreased (0.0136 ± 0.0025 vs 0.0321 ± 0.0013, model vs control, P < 0.01); mitochondria transmembrane potential (239.4638 ± 12.7263 vs 377.5850 ± 16.8119, P < 0.01) was lowered; mitochondrial mass (17.4350 ± 1.9880 vs 31.6738 ± 3.4930, P < 0.01); and [Ca2+]i was increased in liver cells (7.0020 ± 0.5008 vs 10.2050 ± 0.4701, P < 0.01).CONCLUSION: Chronic alcohol intake might lead to broken mitochondria PTP, decreased mitochondria membrane potential and injury, and elevated intracellular Ca2+ production. Ethanol-induced chondriosome injury may be an important mechanism of alcoholic diseases.

关键词： Alcoholic liver disease Chondriosome Apoptosis Ultra microstructure Membrane potentials Permeability transition pore Transmembrane potential chondriosome mass Ca^2+

Investigating microscopic seepage characteristics and fracture effectiveness of tight sandstones:a digital core approach

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Petroleum Science 2021年第1期18卷 173-182页

作者： Jing Li Xiao-Rong Li ming-Shui Song hui-min liu Yong-Cun Feng Chen liu College of Pipeline and Civil Engineering China University of Petroleum(East China)Qingdao 266580ShandongChina College of Safety and Ocean Engineering China University of Petroleum(Beijing)Bejing 102220China Sinopec Shengli Oilfield Company Dongying 257000ShandongChina College of Petroleum Engineering China University of Petroleum(Beijing)Bejing 102220China

Microscopic seepage characteristics are critical for the evaluation of tight sandstone *** this study,a digital core approach integrating microscopic seepage simulation and CT scanning was developed to characterize microscopic seepage and fracture effectiveness(the ratio of micro-fractures that contributes to fluid flow)of tight *** simulations were carried out for characterizations of tight *** results show that the axial permeability of the investigated cylindrical tight sandstone from Junggar Basin in China is 0.460μm~2,while the radial permeability is 0.3723μm~2,and the axial and radial effective fracture ratios are 0.4387 and 0.4806,respectively,indicating that cracks are not fully developed and the connectivity between micro-cracks is *** permeability that is difficult to measure by laboratory experiments can be obtained readily using the proposed method in this *** results provide important information for improving the exploration and development of tight sandstone reservoirs.

关键词： Tight sandstone Digital core approach CT scanning Microscopic seepage characteristics Fracture effectiveness

Reduced graphene oxide supported PdNi alloy nanocrystals for the oxygen reduction and methanol oxidation reactions

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Green Energy & Environment 2018年第4期3卷 375-383页

作者： hui-min liu Shu-He Han Ying-Ying Zhu Pei Chen Yu Chen Key Laboratory of Macromolecular Science of Shaanxi Province Shaanxi Key Laboratory for Advanced Energy Devices School of Materials Science and Engineering Shaanxi Normal University

The research on electrocatalysts with relatively lower price than Pt and excellent electrocatalytic performance for the cathode oxygen reduction reaction(ORR) and anode methanol oxidation reaction(MOR) is vital for the development of direct methanol fuel cells(DMFCs). In this work, we develop a cyanogel-reduction method to synthesize reduced graphene oxide(rGO) supported highly dispersed PdNi alloy nanocrystals(PdNi/rGO) with high alloying degree and tunable Pd/Ni ratio. The large specific surface area and the d-band center downshift of Pd result in excellent activity of Pd4 Ni1/rGO nanohybrids for the ORR. The modification of Pd electronic structure can facilitate the adsorption of CH3 OH on Pd surface and the highly oxophilic property of Ni can eliminate/mitigate the COadsintermediates poisoning, which make PdNi/r GO nanohybrids possess superior MOR activity. In addition, rGO improve the stability of PdNi alloy nanocrystals for the ORR and MOR. Due to high activity and stability for the ORR and MOR, PdNi/rGO nanohybrids are promising to be an available bifunctional electrocatalyst in DMFCs.

关键词： Cyanogel reduction Reduced graphene oxide PdNi alloy nanocrystals Oxygen reduction reaction Methanol oxidation reaction

Diagnosis of erythropoietic protoporphyria with severe liver injury: A case report

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World Journal of Gastroenterology 2019年第7期25卷 880-887页

作者： hui-min liu Guo-Hong Deng Qing Mao Xiao-Hong Wang Department of Infectious Diseases Southwest Hospital Third Military Medical University (Army Medical University)

BACKGROUND Porphyria is a rare disease with complex classification. Erythropoietic protoporphyria(EPP) is an autosomal recessively inherited disease, and most are caused by mutations in the FECH gene. EPP combined with liver injury is even *** SUMMARY This paper reports a case of EPP which was admitted to the hospital with abnormal liver function and diagnosed by repeated questioning of medical history, screening of common causes of severe liver injury, and second generation sequencing of the whole exon genome. We also summarize the clinical characteristics of EPP with liver injury, and put forward some suggestions on EPP to provide a reference for the diagnosis of such rare *** A new mutation locus(c.32_35 dupCCCT) which may be related to the disease was found by detecting the FECH gene in the pedigree of this case.

关键词： Erythropoietic protoporphyria FECH gene Severe liver injury Diagnosis Case report

Analysis of tumor-infiltrating gamma delta T cells in rectal cancer

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World Journal of Gastroenterology 2016年第13期22卷 3573-3580页

作者： Liang Rong Ke Li Rui Li hui-min liu Rui Sun Xiao-Yan liu Department of Gastroenterology the Fifth Affiliated Hospital of Xinjiang Medical University Department of Gastroenterology the First Affiliated Hospital of Soochow University

AIM: To investigate the regulatory effect of Vδ1 T cells and the antitumor activity of Vδ2 T cells in rectal ***: Peripheral blood, tumor tissues and para-carcinoma tissues from 20 rectal cancer patients were collected. Naïve CD4 T cells from the peripheral blood of rectal cancer patients were purified by negative selection using a Naive CD4+ T Cell Isolation Kit II (Miltenyi Biotec). Tumor tissues and para-carcinoma tissues were minced into small pieces and digested in a triple enzyme mixture containing collagenase type IV, hyaluronidase, and deoxyribonuclease for 2 h at room temperature. After digestion, the cells were washed twice in RPMI1640 and cultured in RPMI1640 containing 10% human serum supplemented with L-glutamine and 2-mercaptoethanol and 1000 U/mL of IL-2 for the generation of T cells. Vδ1 T cells and Vδ2 T cells from tumor tissues and para-carcinoma tissues were expanded by anti-TCR γδ antibodies. The inhibitory effects of Vδ1 T cells on naïve CD4 T cells were analyzed using the CFSE method. The cytotoxicity of Vδ2 T cells on rectal cancer lines was determined by the LDH ***: The percentage of Vδ1 T cells in rectal tumor tissues from rectal cancer patients was significantly increased, and positively correlated with the T stage. The percentage of Vδ2 T cells in rectal tumor tissues from rectal cancer patients was significantly decreased, and negatively correlated with the T stage. After culture for 14 d with 1 μg/mL anti-TCR γδ antibodies, the percentage of Vδ1 T cells from para-carcinoma tissues was 21.45% ± 4.64%, and the percentage of Vδ2 T cells was 38.64% ± 8.05%. After culture for 14 d, the percentage of Vδ1 T cells from rectal cancer tissues was 67.45% ± 11.75% and the percentage of Vδ2 T cells was 8.94% ± 2.85%. Tumor-infiltrating

关键词： Rectal cancer T cells Vδ 1 T cells Vδ 2 T cells Foxp3 Cytotoxicity

Formation and characterization of core-shell CL-20/TNT composite prepared by spray-drying technique

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Defence Technology（防务技术） 2021年第6期17卷 1936-1943页

作者： Chang-gui Song Xiao-dong Li Yue Yang hui-min liu Ying-xin Tan Jing-yu Wang School of Environment and Safety Engineering North University of ChinaTaiyuan030051China

The core-shell 2,4,6,8,10,12-Hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane/2,4,6-Trinitrotoluene(CL-20/TNT)composite was prepared by spray-drying method in which sensitive high energy explosive(CL-20)was coated with insensitive explosive(TNT).The structure and properties of different formulations of CL-20/TNT composite and CL-20/TNT mixture were characterized by scanning electron microscopy(SEM),Transmission electron microscopy(TEM),Laser particle size analyzer,X-ray photoelectron spectroscopy(XPS),X-ray diffraction(XRD),differential scanning calorimetry(DSC),impact sensitivity test and detonation *** results of SEM,TEM,XPS and XRD show that e-CL-20 particles are coated by *** the ratio of CL-20/TNT is 75/25,core-shell structure is well formed,and thickness of the shell is about 20e30 *** the analysis of heat and impact show that with the increase of TNT content,the TNT coating on the core-shell composite material can not only catalyze the thermal decomposition of core material(CL-20),but also greatly reduce the impact *** with the CL-20/TNT mixture(75/25)at the same ratio,the characteristic drop height of core-shell CL-20/TNT composite(75/25)increased by 47.6%and the TNT coating can accelerate the nuclear decomposition in the CL-20/TNT ***,the preparation of the core-shell composites can be regarded as a unique means,by which the composites are characterized by controllable decomposition rate,high energy and excellent mechanical sensitivity and could be applied to propellants and other fields.

关键词： Energetic materials CL-20(2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane) TNT(2,4,6-Trinitrotoluene) Spray-drying method Core-shell structure

Differential neuronal reprogramming induced by NeuroD1 from astrocytes in grey matter versus white matter

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Neural Regeneration Research 2020年第2期15卷 342-351页

作者： min-hui liu Wen Li Jia-Jun Zheng Yu-Ge Xu Qing He Gong Chen Guangdong-HongKong-Macao Institute of CNS Regeneration (GHMICR) Jinan UniversityGuangzhouGuangdong ProvinceChina Department of Biology The Huck Institutes of Life SciencesThe Pennsylvania State UniversityUniversity ParkPAUSA

A new technology called in vivo glia-to-neuron conversion has emerged in recent years as a promising next generation therapy for neural regeneration and repair. This is achieved through reprogramming endogenous glial cells into neurons in the central nervous system through ectopically expressing neural transcriptional factors in glial cells. Previous studies have been focusing on glial cells in the grey matter such as the cortex and striatum, but whether glial cells in the white matter can be reprogrammed or not is unknown. To address this fundamental question, we express NeuroD1 in the astrocytes of both grey matter(cortex and striatum) and white matter(corpus callosum) to investigate the conversion efficiency, neuronal subtypes, and electrophysiological features of the converted neurons. We discover that NeuroD1 can efficiently reprogram the astrocytes in the grey matter into functional neurons, but the astrocytes in the white matter are much resistant to neuronal reprogramming. The converted neurons from cortical and striatal astrocytes are composed of both glutamatergic and GABAergic neurons, capable of firing action potentials and having spontaneous synaptic activities. In contrast, the few astrocyte-converted neurons in the white matter are rather immature with rare synaptic events. These results provide novel insights into the differential reprogramming capability between the astrocytes in the grey matter versus the white matter, and highlight the impact of regional astrocytes as well as microenvironment on the outcome of glia-toneuron conversion. Since human brain has large volume of white matter, this study will provide important guidance for future development of in vivo glia-to-neuron conversion technology into potential clinical therapies. Experimental protocols in this study were approved by the Laboratory Animal Ethics Committee of Jinan University(approval No. IACUC-20180321-03) on March 21, 2018.

关键词： astrocyte conversion efficiency corpus callosum cortex grey matter in vivo cell conversion NeuroD1 neuron reprogramming striatum white matter

Pencil painting like preparation for flexible thermoelectric material of high-performance p-type Na_(1.4)Co_(2)O_(4) and novel n-type NaxCo_(2)O_(4)

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Journal of Materiomics 2021年第5期7卷 1153-1160页

作者： Zhen Tian Jun Wang Xinba Yaer hui-Jun Kang Xiao-Huan Wang hui-min liu De-zhi Yang Tong-min Wang School of Materials Science and Engineering Inner Mongolia University of TechnologyNo.49 Aimin StreetXincheng DistrictHohhotInner Mongolia Autonomous Region010051China Key Laboratory of Solidification Control and Digital Preparation Technology(Liaoning Province) School of Materials Science and EngineeringDalian University of TechnologyDalian116024China International Mongolian Hospital HohhotInner Mongolia Autonomous Region010065China

Flexible thermoelectric materials are presented with potential applications in electronic devices and energy conversion due to their convenient preparation,good flexibility,and various ***,as ductility is rarely observed in inorganic semiconductors and ceramic insulators,reports on applications of inorganic oxide materials in flexible thermoelectric materials are ***,we report a new method for the synthesis of a flexible Na_(1.4)Co_(2)O_(4) thermoelectric material based on Na_(1.4)Co_(2)O_(4) bulk materials,which are prepared by a self-flux method and painted on print *** coefficient and power factor of the obtained thermoelectric material are 78-102 μVK^(-1) and 159e223 mWm^-(1)K^(-2),respectively,in a temperature range of 303-522 K,which are superior to those values of other conductive polymers and their *** interestingly,the n-type Na_(1.4)Co_(2)O_(4) flexible material is obtained in the painting process at higher pressure with Seebeck coefficients of109 to183 μVK^(-1) in a temperature range of 303-522 *** convenient preparation method of these novel flexible thermoelectric materials may be expanded to the synthesis of other flexible thermoelectric materials,which will be the focus of future work.

关键词： Na_(1.4)Co_(2)O_(4) Flexible thermoelectric material Print paper Layered oxide n-type Na_(1.4)Co_(2)O_(4)

ISCHEMIC PRECONDITIONING RELIEVES ISCHEMIA/REPERFUSION INJURY OF HIPPOCAMPUS NEURONS IN RAT BY INHIBITING p53 AND BAX EXPRESSIONS

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Chinese Medical Sciences Journal 2007年第2期22卷 123-127页

作者： hui-min liu Jing-xin Li Lian-bi Chen Institute of Physiology School of Medicine Shandong University Jinan 250012 Department of Physiology Shandong University of Traditional Chinese Medicine Jinan 250014

Objective To examine whether ischemic preconditioning (IPC) can protect neuron against delayed death in CA1 subfield of hippocampus following reperfusion of a lethal ischemia in rats, and explore the role of p53 and bax in this process. Methods We examined the effect of IPC on delayed neuron death, neuron apoptosis, expressions of p53 and bax gene in the CA1 area of hippocampus in the rats using HE staining, flow cytometry, RT-PCR, and immunohistochemistry technique. Results IPC enhanced the quantity of survival cells in the CA1 region of hippocampus (216±9 cells/0.72 mm2 vs. 30±5 cells/0.72 mm2, P<0.01), decreased the percentages of apoptotic neurons of hippocampus caused by ischemia/reperfusion (2.06%±0.21% vs. 4.27%±0.08%, P<0.01), and weakened the expressions of p53 and bax gene of hippocampus compared with ischemia/reperfusion without IPC. Conclusion IPC can protect the neurons in the CA1 region of hippocampus against apoptosis caused by ische- mia/reperfusion, and this process may be related to the reduced expressions of p53 and bax.

关键词： rat hippocampus ischemic preconditioning p53 bax