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Genome-wide transcriptional analysis of maize endosperm in response to ae wx double mutations

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Journal of Genetics and Genomics 2010年第11期37卷 749-762页

作者： Xiang Li Guang hui chen wei Yang Zhang Xiansheng Zhang State Key Laboratory of Crop Biology College of Life Sciences Shandong Agricultural University Taian 271018 China

Starch biosynthesis is important during endosperm development. Much has been known for the regulation of gene expression involved in starch synthesis, less information is available on the genome-wide expression profiles as a consequence of impaired starch synthesis. In this study, we examined the transcriptional responses through microarray analysis in an ae wx double-mutant with loss-of-function starch branching enzyme IIb （SBEIIb） and granule-bound starch synthase I （GBSSI）. Through Gene Ontology enrichment analysis, we identified differentially expressed genes （DEGs） involved in chromatin organization and lipid transport. The DEGs also include alcohol dehydrogenase genes and pyruvate decarboxylase genes involved in sugar metabolism. In summary, the ae wx double mutations caused pleiotropic effects and transcriptional changes for a number of genes involved in metabolism, cellular response and organization. Therefore, a block in starch synthesis triggers transcriptional responses to favour the flux of excess carbohydrates into glycolysis, pentose phosphate pathway, and cell wall biosynthesis, but not toward the synthesis of alternative storage compounds.

关键词： transcriptional regulation starch biosynthesis carbohydrate metabolism

A Micro-Payment Based Isolation ofMisbehavior Secure Routing for Sensor Network

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Wuhan University Journal of Natural Sciences 2005年第1期10卷 93-97页

作者： LIhui weiwei chenKe-fei CryptographyandInformationSecurityLaboratory ShanghaiJiaotongUniversityShanghai200030China

In this paper, we present a Micro-payment based Isolation of Misbehavior(MIM) secure routing protocol. The protocol contains three parts: micro-payment scheme, routingdiscovery and malicious nodes detection The micro-payment scheme proposed in MIM is the firstvirtual currency system that can be suit lor sensor network environment, Based on the micro-paymentscheme, we device an energy aware routing protocol, which forwards packets by auction. Furthermore,the base station can detect and isolate misbehave or non-cooptrare nodes according to the routinginformation.

关键词： secure routing wireless sensor network micro-payment

Clinical value of continuous administration of sorafenib in combination with modified transarterial chemoembolization in patients with unresectable hepatocellular carcinoma

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Chinese Medical Journal 2013年第2期126卷 385-386页

作者： HUANG Yong-hui chen wei LI Jia-ping chen Bin YANG Jian-yong Department of Interventional Radiology First Affiliated Hospitalof Sun Yat-sen University Guangzhou Guangdong 510080 China

Hepatocellular carcinoma （HCC） is a major threat to human health in China with high morbidity andmortality. Surgical resection and liver transplantation are the most promising treatments for HCC. The majority of patients, however, are not eligible for surgery due to 1 advanced tumor, multifocal disease or poor liver reserve. Recently, transarterial chemoembolization （TACE） improved survival compared with best supportive care in two individual clinical trials.2 Sorafenib is a multikinase inhibitor that targets both tumor cell proliferation and angiogenesis. The current standard of practice is to treat BCLC stage B HCC with TACE alone and more advanced （BCLC stage C） HCC with sorafenib only.1

关键词： hepatocellular carcinoma, progression-free survival, sorafenib transarterial chemoembolization

Beta-nerve growth factor promotes neurogenesis and angiogenesis during the repair of bone defects

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Neural Regeneration Research 2015年第7期10卷 1159-1165页

作者： wei-hui chen Chuan-qing Mao Li-li Zhuo Joo L.Ong Department of Oral Surgery Union Hospital Fujian Medical University Department of Biomedical Engineering University of Texas at San Antonio

We previously showed that the repair of bone defects is regulated by neural and vascular signals. In the present study, we examined the effect of topically applied β-nerve growth factor（β-NGF） on neurogenesis and angiogenesis in critical-sized bone defects filled with collagen bone substitute. We created two symmetrical defects, 2.5 mm in diameter, on either side of the parietal bone of the skull, and filled them with bone substitute. Subcutaneously implanted osmotic pumps were used to infuse 10 μgβ-NGF in PBS（β-NGF ＋ PBS） into the right-hand side defect, and PBS into the left（control） defect, over the 7 days following surgery. Immunohistochemical staining and hematoxylin-eosin staining were carried out at 3, 7, 14, 21 and 28 days postoperatively. On day 7, expression of β III-tubulin was lower on the β-NGF ＋ PBS side than on the control side, and that of neurofilament 160 was greater. On day 14, β III-tubulin and protein gene product 9.5 were greater on the β-NGF ＋ PBS side than on the control side. Vascular endothelial growth factor expression was greater on the experimental side than the control side at 7 days, and vascular endothelial growth factor receptor 2 expression was elevated on days 14 and 21, but lower than control levels on day 28. However, no difference in the number of blood vessels was observed between sides. Our results indicate that topical application of β-NGF promoted neurogenesis, and may modulate angiogenesis by promoting nerve regeneration in collagen bone substitute-filled defects.

关键词： nerve regeneration β-nerve growth factor collagen angiogenesis protein gene product 9.5 vascular endothelial growth factor β III-tubulin neural regeneration

A Multi-objective Optimal Approach to Automated Construction of Sacrificial Multi-piece Molds

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Journal of Shanghai Jiaotong university(Science) 2008年第5期13卷 542-546页

作者：王会凤周雄辉陈巍 National Die and Mold CAD Engineering Research Center Shanghai Jiaotong University

Sacrificial multi-piece molds can be used for producing complex parts. To obtain the optimal design of molds automatically, a multi-objective optimal approach is proposed. Mold pieces number, material utilization and partitioning area are taken as the objective functions, and the machinability of each mold piece is taken as constraint condition. Genetic algorithm (GA) is adopted to realize global optimization of partitioning process. Each mold piece in optimal scheme can be manufactured by milling and drilling operations, which reduce the tooling cost and shorten product cycle obviously. Using the proposed approach, mold design can be significantly automated for making complex parts.

关键词： sacrificial multi-piece molds multi-objective optimization machinability genetic algorithm (GA)

Predictors of the outcomes of acute-on-chronic hepatitis B liver failure

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World Journal of Gastroenterology 2012年第36期18卷 5078-5083页

作者： Hsiu-Lung Fan Po-Sheng Yang hui-wei chen Teng-wei chen De-Chuan Chan Chi-Hong Chu Jyh-Cherng Yu Shih-Ming Kuo Chung-Bao Hsieh Division of General Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical Center Division of General Surgery Department of SurgeryMackay Memorial Hospital School of Medicine University of PittsburghPittsburghPA 15261United States Division of Pediatric Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical Center

AIM： To identify the risk factors in predicting the out- come of acute-on-chronic hepatitis B liver failure pa- tients. METHODS： We retrospectively divided 113 patients with acute-on-chronic liver failure-hepatitis B virus （ACLF-HBV） and without concurrent hepatitis C or D virus infection and hepatocellular carcinoma into two groups according to their outcomes after anti-HBV therapy. Their demographic, clinical, and biochemical data on the day of diagnosis and after the first week of treatment were analyzed using the Mann-Whitney U test, Fisher＇s exact test, and a multiple logistic regres- sion analysis. RESULTS： The study included 113 patients （87 men and 26 women） with a mean age of 49.84 years. Fifty- two patients survived, and 61 patients died. Liver failure （85.2%）, sepsis （34.4%）, and multiple organ failure （39.3%） were the main causes of death. Mul- tivariate analyses showed that Acute Physiology and Chronic Health Evaluation （APACHE） Ⅱ scores ≥ 12 [odds ratio （OR） = 7.160, 95% CI： 2.834-18.092, P 〈 0.001] and positive blood culture （OR = 13.520, 95% CI： 2.740-66.721, P = 0.001） on the day of diagnosis and model for end-stage liver disease （MELD） scores 28 （OR = 8.182, 95% CI： 1.884-35.527, P = 0.005） after the first week of treatment were independent predictors of mortality. CONCLUSION： APACHE II scores on the day of diag- nosis and MELD scores after the first week of anti-HBV therapy are feasible predictors of outcome in ACLF- HBV patients.

关键词： Lamivudine Liver failure Hepatitis B virus Acute Physiology and Chronic Health Evaluation ]]score Model for end-stage liver disease scores

Construction of a soybean linkage map using an F_2 hybrid population from a cultivated variety and a semi-wild soybean

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Chinese Science Bulletin 1997年第12期42卷 1033-1037页

作者： ZHANG Deshui, DONG wei, hui Dongwei, chen Shouyi and ZHUANG Bingchang1. Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2 Soybean Institute, Jilin Academy of Agricultural Sciences, Jiltn 136100, China Plant Biotechnolcgy Laboratory Institute of Genetics Chinese Academy of Sciences Beijing China Soybean Institute Jilin Academy of Agricultural Sciences Jilin China

ALTHOUGH soybean is one of world’s leading sources of seed oil and seed protein, the develop-ment of its genetic map has lagged behind that of other crops such as rice and maize. The maincauses for this can be attributed to the lack of genetic diversity among soybean accessions, thepaucity of classical markers, the big size and complexity of the genome, and the small size anddifficulty in distinguishing of the chromosomes. The classical genetic map of

关键词： soybean F2 population molecular linkage map.

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具有降糖作用的鼠李糖乳杆菌生物学特性研究

食品工业科技 2016年第21期37卷 162-165,171页

作者：陈佩党辉王伟陈卫陕西广播电视大学陕西西安710119 陕西理工学院生物科学与工程学院陕西汉中723001 江南大学食品科学与技术国家重点实验室江苏无锡214122 陕西师范大学食品工程与营养科学学院陕西西安710119

摘要：本研究旨在评价一株具有降糖作用的鼠李糖乳杆菌的生物学特性。结果表明，该菌株生长较快，2h进入生长对数期，14h后进入稳定期，最适生长pH为5—7，最适生长温度为37℃，最适接种量为1％-2％（V／V）。此外，该菌株在强酸条件下... 详细信息

摘要：本研究旨在评价一株具有降糖作用的鼠李糖乳杆菌的生物学特性。结果表明，该菌株生长较快，2h进入生长对数期，14h后进入稳定期，最适生长pH为5—7，最适生长温度为37℃，最适接种量为1％-2％（V／V）。此外，该菌株在强酸条件下可以生长，对人工胃肠液都具有很好的耐受性，耐受胆盐的延迟时间为0．86h，在NaCI浓度为9％（W／V）的培养条件下仍可以存活。更为重要的是，这株菌对Caco-2细胞具有较强的粘附能力。综上所述：该鼠李糖乳杆菌菌株可作为一株具有潜在降糖作用的发酵菌株，用以开发具有降糖作用的新型发酵产品。

关键词：降糖鼠李糖乳杆菌生物学特性

Brain region-specific roles of brain-derived neurotrophic factor in social stress-induced depressive-like behavior

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Neural Regeneration Research 2025年第1期20卷 159-173页

作者： Man Han Deyang Zeng wei Tan Xingxing chen Shuyuan Bai Qiong Wu Yushan chen Zhen wei Yufei Mei Yan Zeng Brain Science and Advanced Technology Institute Wuhan University of Science and TechnologyWuhanHubei ProvinceChina Geriatric Hospital Affiliated to Wuhan University of Science and Technology WuhanHubei ProvinceChina School of Public Health Wuhan University of Science and TechnologyWuhanHubei ProvinceChina

Brain-derived neurotrophic factor is a key factor in stress adaptation and avoidance of a social stress behavioral *** studies have shown that brain-derived neurotrophic factor expression in stressed mice is brain region–specific,particularly involving the corticolimbic system,including the ventral tegmental area,nucleus accumbens,prefrontal cortex,amygdala,and *** how brain-derived neurotrophic factor participates in stress processing in different brain regions will deepen our understanding of social stress *** this review,we discuss the expression and regulation of brain-derived neurotrophic factor in stress-sensitive brain regions closely related to the pathophysiology of *** focused on associated molecular pathways and neural circuits,with special attention to the brain-derived neurotrophic factor–tropomyosin receptor kinase B signaling pathway and the ventral tegmental area–nucleus accumbens dopamine *** determined that stress-induced alterations in brain-derived neurotrophic factor levels are likely related to the nature,severity,and duration of stress,especially in the above-mentioned brain regions of the corticolimbic ***,BDNF might be a biological indicator regulating stress-related processes in various brain regions.

关键词： amygdala chronic mild stress chronic social defeat stress corticolimbic system depression hippocampus medial prefrontal cortex nucleus accumbens social stress models ventral tegmental area