检索结果-南通市图书馆

Prognostic impact of dissected lymph node count on patients with node-negative gastric cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2009年第31期15卷 3926-3930页

作者： Chang-Ming Huang Jian-Xian Lin Chao-hui zheng ping Li Jian-Wei Xie Bi-Juan Lin hui-Shan Lu Department of Ontology Affiliated Union Hospital Fujian Medical University No. 29 Xinquan Road Fuzhou 350001 Fujian Province China

AIM:To investigate the long-term effect of the number of resected lymph nodes (LNs) on the prognosis of patients with node-negative gastric ***: Clinical data of 211 patients with gastric cancer, without nodal involvement, were analyzed retrospectively after D2 radical operation. We analyzed the relationship between the number of resected LNs with the 5-year survival, the recurrence rate and the post-operative complication ***: The 5-year survival of the entire cohort was 82.2%. The total number of dissected LNs was one of the independent prognostic factors. Among patients with comparable depth of invasion, the larger the number of resected LNs, the better the survival (P0.01). The post-operative complication rate was 10.9% and was not signif icantly correlated with the number of dissected LNs (P>0.05).CONCLUSION: For node-negative gastric cancer, sufficient number of dissected LNs is recommended during D2 lymphadenectomy, to improve the long-term survival and reduce the recurrence. Suitable increments of the dissected LN count would not increase the post-operative complication rate.

关键词： Stomach neoplasms Lymph nodes Gastrectomy Lymphadenectomy Prognosis

Capillary electrophoresis with laser-induced fluorescence detection of main polyamines and precursor amino acids in saliva

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2014年第4期25卷 640-644页

作者： Zhuo Li Yang Zhang Fang-Hong Tong Ting-Ting Jiang hui-ping zheng Jian-Nong Ye Qing-Cui Chu Department of Chemistry East China Normal University

A hollow-fiber liquid-phase microextraction （HF-LPME） method has been developed for the purification and preconcentration of biogenic polyamines and their precursor amino acids in human saliva. Putrescine （Put）, cadaverine （Cad）, spermidine （Spe）, ornithine （Orn）, lysine （Lys）, and arginine （Arg） were determined by the CE-LIF detection after microextraction. Several factors that affect extraction efficiency, separation, and detection were investigated. Under the optimum conditions, six analytes could achieve baseline separation within 30 min, exhibiting a linear calibration at three orders of magnitude （r2 〉 0.998）; the obtained enrichment factors of HF-LPME were between 19 （for Orn） and 2] 8 （for Cad）, and the LODs were in the range of 0.0072-0.26 nmol/L. The proposed HF-LPME/CE-LIF method has been successfully applied for the sensitive analyses of the real-world saliva samples collected from healthy volunteers and different patients with oral diseases, providing a potential method for primary non-invasive diagnosis of some oral diseases.

关键词： Polyamine Saliva Capillary electrophoresis Hollow-fiber liquid-phase microextraction

Possible roles of proto-oncogene c-src on the initiation growth of rat primordial follicles

在线全文

学校读者我要写书评

暂无评论

Possible roles of proto-oncogene c-src on the initiation gro...

Genes that involvement in introducing progesterone,estradiol,epidermal growth factor and growth differentiation factor-9 to regulate the initiation growth of primordial follicles

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Xiao-Yu DU,Dan-Feng TANG,Yuan-hui XU,Li-ping zheng,Yue-hui zheng~* Department of Physiology Reproduction,Nanchang University Medical College,Nanchang 330006,China

＜正＞Objective:Primordial follicle initiation and growth are the vital center of animal reproduction,but the mechanisms are *** aim of this article is to explore the expression,distribution of c-src mRNA and protein in the ovaries as primordial follicle development and the role and mechanisms of c-src on the growth of primordial ***:Ovaries were collected from 2-day-old rats,and cultured in Waymouth culture system;the morphous and the quantity of every stage follicles of ovaries cultured for 0 d,4 d and 8 d were observed by histological sections stained with *** situ hybridization,RT-PCR,western blot and immunohistochemistry were performed to assess the expressions of c-src mRNA and protein during the initiation growth of primordial *** interference was used to observe the effect of proto-oncogene c-src on the initiation of primordial follicles;and inhibitors such as MAPK inhibitor（PD98059）,PKC inhibitor（calphostin）,PI-3K inhibitor（LY294002） were used to investigate the upstream or downstream relationship with c-src in the initiation of primordial follicle ***:Immunohistochemical technique,insitu hybridization and RT-PCR showed that c-src mRNA and protein existed in primordial follicles,their expression levels increased as the primordial follicles growth,c-src mRNA and proteins distributed in oocyte cytoplasm and granulosa *** interfering showed that the development of primordial follicles were remarkablely suppressed after c-src siRNA transfection and corresponding,c-src mRNA and protein,the levels of p-PKC,p-ERK1/2 and p-PI3K in follicles were significantly *** initiation of primordial follicles delayed obviously,but c-src mRNA and protein showed no significant change after PD98059,LY294002 and calphostin effect;p-ERK1/2 level in follicles decreased after PD98059,calphostin and LY294002 effect;p-PKC level in follicles decreased after calphostin and LY294002 effect but no change in PD98059;p-PI3K le

关键词： src primordial follicle RNA interference signaling pathway

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Genes that involvement in introducing progesterone,estradiol...

中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Dan-Feng TANG,Xiao-Yu DU,Yuan-hui XU,Li-ping zheng,Yue-hui zheng~* Department of Physiology Reproduction,Nanchang University Medical College,Nanchang 330006,China

Background:Primordial follicle assembly and the rate of primordial to primary follicle transition influence the size,of primordial follicle *** primordial follicle development can lead to pathologies such as premature ovarian *** aim of our research was to study the effects of four medicines during the initiation growth of primordial ***:Ovaries were collected from 2-day-old SD rats,after 8 d cultured in the Waymouth culture system which was treated with either 50 ng/mL epidermal growth factor(EGF),200 ng/mL growth differentiation factor-9(GDF-9),10 mol/mL estradiol(E),10 mol/mL progesterone(P),or *** morphous and quantity of every stage follicles of ovaries cultured for 8 d were observed by histological section stained with hematoxylin after ovaries were *** gene expression levels were studied using Illumina rat whole genome expression ***:The quantities of primary follicles and second follicles in the EGF and GDF-9 groups were higher than those in the control group(P+20) including proto-oncogenes A2m,and 79 down-regulated genes(Diffscore<-20) including proto-oncogenes Mycn,between EGF group and control *** the expression array analysis revealed that 178 differentially expressed genes in the GDF-9 group,no known proto-oncogene among *** with control groups,there were 121 and 235 differentially expressed genes in P and E groups,respectively. Interestingly,proto-oncogene Mycn was down-regulated in the E ***:In the Waymouth culture system,EGF and GDF-9 can promote the growth of primordial follicles,P and E can inhibit the growth of primordial *** find a number of proto-oncogenes,such as A2m and Mycn,potentially involved in primordial follicles development by the microarray app

关键词： genes microarray primordial follicle progesterone estradiol epidermal growth factor growth differentiation factor-9

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

扰动情况下一类混沌系统的观测器同步

物理学报 2003年第2期52卷 276-280页

作者：关新平何宴辉范正平燕山大学电气工程学院秦皇岛066004

研究了存在外界扰动情况下一类混沌系统的同步问题 ,混沌系统非线性项无需满足输出反馈形式 .首先对系统进行变换得到未受扰降价混沌系统 ,然后基于非线性观测器的思想构造同步观测系统实现了混沌同步 ,通过仿真证实了该方法的有效性 .

关键词：扰动混沌系统混沌同步观测器

The effects of morphine on the presynaptic glutamate release in the pyramidal neurons of the basolateral amygdala and its mechanism

在线全文

学校读者我要写书评

暂无评论

The effects of morphine on the presynaptic glutamate release...

Tumor size as a prognostic factor in patients with advanced gastric cancer in the lower third of the stomach

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jin-hui YANG,ping zheng State Key Laboratory of Medical Neurobiology,Shanghai Medical College and Institutes of Brain Science,Fudan University,Shanghai 200032,China

By sustained activation of opioid receptors,chronic opioids cause analgesic tolerance,physical dependence,and opioid addiction,for which an effective treatment is still ***,as a part of limbic system,plays an important role in opioid *** this study,we using patch clamp recording method combined with pharmacological approaches investigated chronic morphine-induced adaptations in glutamatergic synaptic transmission in neurons of the basolateral amygdala(BLA).Chronic morphine significantly increased glutamate synaptic transmission in pyramidal neurons in BLA containingμ-opioid *** naive rats,DAMGO,μ-opioid receptor’s activator,could inhibit the release of presynaptic glutamate,while DAMGO would lost this inhibitory effect in chronic morphine *** found that in saline rats,DAMGO’s effect was blocked by AACOCF,an inhibitor of phospholipase A and 4-AP,which blocks the presynaptic voltage-dependent potassium channels regulated by metabolites of the PLA *** PKA didn’t play any roles in naive ***,we confirmed that in chronic morphine group,PLA2-AA-Kv pathway was downregulated and PKA was upregulated,which were involved in the effect of DAMGO on presynaptic glutamate *** job provided the new mechanism of opioid receptors in morphine treatment for further clinical perspective.

关键词： morphine opioid receptors basolateral amygdale glutamate release addiction signal transduction pyramidal neuron

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2012年第38期18卷 5470-5475页

作者： Hong-Mei Wang, Chang-Ming Huang, Chao-hui zheng, ping Li, Jian-Wei Xie, Jia-Bin Wang, Jian-Xian Lin, Jun Lu, Department of Gastric Surgery, Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, Fujian Province, China Author contributions: Wang HM and Huang CM conceived of the study, analyzed the data, and drafted the manuscript zheng CH, Li P and Xie JW helped revise the manuscript critically for important intellectual content Wang JB, Lin JX and Lu J helped collect data and design the study and all authors read and approved the final manuscript. Department of Gastric Surgery Affiliated Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China.

AIM: To explore the impact of tumor size on outcomes in patients with advanced gastric cancer in the lower third of the stomach. METHODS: We retrospectively analyzed the clinical records of 430 patients with advanced gastric cancer in the lower third of the stomach who underwent distal subtotal gastrectomy and D2 lymphadenectomy in our hospital from January 1998 to June 2004. Receiver-operating characteristic (ROC) curve analysis was used to determine the appropriate cutoff value for tumor size, which was measured as maximum tumor diameter. Based on this cutoff value, patients were divided into two groups: those with large-sized tumors (LSTs) and those with small-sized tumors (SSTs). The correlations between other clinicopathologic factors and tumor size were investigated, and the 5-year overall survival (OS) rate was compared between the two groups. Potential prognostic factors were evaluated by univariate KaplanMeier survival analysis and multivariate Cox's propor-tional hazard model analysis. The 5-year OS rates in the two groups were compared according to pT stage and pN stage. RESULTS: The 5-year OS rate in the 430 patients with advanced gastric cancer in the lower third of the stomach was 53.7%. The mean ± SD tumor size was 4.9 ± 1.9 cm, and the median tumor size was 5.0 cm. ROC analysis indicated that the sensitivity and specificity results for the appropriate tumor size cutoff value of 4.8 cm were 80.0% and 68.2%, respectively (AUC=0.795, 95%CI: 0.751-0.839, P=0.000). Using this cutoff value, 222 patients (51.6%) had LSTs (tumor size ≥ 4.8 cm) and 208 (48.4%) had SSTs (tumor size<4.8 cm). Tumor size was significantly correlated with histological type (P=0.039), Borrmann type (P=0.000), depth of tumor invasion (P=0.000), lymph node metastasis (P=0.000), tumor-nodes metastasis stage (P=0.000), mean number of metastatic lymph nodes (P=0.000) and metastatic lymph node ratio (P=0.000). Patients with LSTs had a significantly lower 5-year OS rate than those with SS

关键词： Gastric carcinoma Prognosis Receiver operating characteristic curve The lower third of stomach Tumor size

Physarum polycephalum-inspired adaptive optimization design of artificial microtubular networks

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2025年

作者： Si-Min Zeng Di-Wei zheng Ting Pan Xing-Lan Ding Yun Zhang Chi-hui Yang Si-Xue Cheng Xian-zheng Zhang Key Laboratory of Biomedical Polymers of Ministry of Education&Department of Chemistry Wuhan University

Natural biological microtubular networks have undergone adaptive evolutionary selection and may offer viable solutions to design challenges in artificial microtubular networks. The plasmodium of the slime mold Physarum polycephalum(P. polycephalum) extends continuously to form a protoplasmic microtubular network structure, directly connecting food *** simulations revealed that the formation of this adaptive P. polycephalum microtubular network could be captured by a mathematical algorithm. Inspired by the P. polycephalum microtubular networks, we propose an adaptive optimization design method for artificial microtubular networks. Specifically, we utilized hydrogels with biodegradable and tissue-adhesive properties to replicate the P. polycephalum microtubular networks via photomask. In Rhodamine B diffusion and glucosecatalyzed reaction experiments, we found that the P. polycephalum microtubular networks exhibited significantly enhanced efficiency compared to vascular and artificial networks. Furthermore, we demonstrated the potential for uric acid(UA) degradation of the hydrogels with a real P. polycephalum microtubular network loaded with urate oxidase(UOx) in a rodent model of hyperuricemia. And this network achieved more than double the effect of the artificial network. This underscores the potential of natural microtubular networks to replace artificial microtubular networks.

关键词：

The role of axon guidance molecules in the pathogenesis of epilepsy

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第5期20卷 1244-1257页

作者： zheng Liu Chunhua Pan Hao Huang Department of Neurology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Current treatments for epilepsy can only manage the symptoms of the condition but cannot alter the initial onset or halt the progression of the disease. Consequently, it is crucial to identify drugs that can target novel cellular and molecular mechanisms and mechanisms of action. Increasing evidence suggests that axon guidance molecules play a role in the structural and functional modifications of neural networks and that the dysregulation of these molecules is associated with epilepsy susceptibility. In this review, we discuss the essential role of axon guidance molecules in neuronal activity in patients with epilepsy as well as the impact of these molecules on synaptic plasticity and brain tissue remodeling. Furthermore, we examine the relationship between axon guidance molecules and neuroinflammation, as well as the structural changes in specific brain regions that contribute to the development of epilepsy. Ample evidence indicates that axon guidance molecules, including semaphorins and ephrins, play a fundamental role in guiding axon growth and the establishment of synaptic connections. Deviations in their expression or function can disrupt neuronal connections, ultimately leading to epileptic seizures. The remodeling of neural networks is a significant characteristic of epilepsy, with axon guidance molecules playing a role in the dynamic reorganization of neural circuits. This, in turn, affects synapse formation and elimination. Dysregulation of these molecules can upset the delicate balance between excitation and inhibition within a neural network, thereby increasing the risk of overexcitation and the development of epilepsy. Inflammatory signals can regulate the expression and function of axon guidance molecules, thus influencing axonal growth, axon orientation, and synaptic plasticity. The dysregulation of neuroinflammation can intensify neuronal dysfunction and contribute to the occurrence of epilepsy. This review delves into the mechanisms associated with

关键词： axon guidance drug-resistant epilepsy epilepsy nerve regeneration nervous system diseases neural pathways neuroinflammatory diseases neuronal plasticity neurons synaptic remodeling