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A Sandwich-type Lateral Flow Strip Using a Split, Single Aptamer for Point-of-Care Detection of Cocaine

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Journal of Analysis and Testing 2022年第2期6卷 120-128页

作者： Le Jing Chong-Yu Xie Qian-Qian Li hui-fang yao Mei-Qing Yang hui Li Fan Xia Shao-Guang Li State Key Laboratory of Biogeology and Environmental Geology Engineering Research Center of Nano-Geomaterials of Ministry of EducationFaculty of Materials Science and ChemistryChina University of GeosciencesWuhan 430074China Hubei University of Police Wuhan 430034China

The sandwich-type lateral fl ow assays relying on dual aptamers with high sensitivity and specifi city have been broadly ***,it is unlikely to match a pair of specifi c aptamers that can bind a small molecular target(e.g.,cocaine)simultaneously due to the steric *** response,we herein introduced the strategy of“one divides into two”into the construction of sandwich-type lateral fl ow strip assay(LFSA).Specifi cally,we split a single cocaine-recognizing aptamer into two segments,either of which was conjugated with gold nanoparticles(AuNPs)or labeled with biotin,serving as signal probe and capture probe,*** the presence of the target molecule,a ternary sandwich complex comprised of the two halves of the aptamer and the target *** sandwich-type LFSA exhibited an excellent nonlinear logarithmic response in the range from 10μmol/L to 5 mmol/L with R^(2)=*** sensitive on-site detection of cocaine in artifi cial biological samples including urine and sweat was achieved within 15 min,with the visual limit of detection as low as 50μmol/L for urine and 200μmol/L for sweat,and the recovery rates of 83.6-107.4%.

关键词： Lateral flow strip Split aptamer Sandwich-type AuNPs Cocaine

The mRNA expression patterns of tumor necrosis factor-a and TNFR-I in some vital organs after thermal injury

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World Journal of Gastroenterology 2003年第5期9卷 1038-1044页

作者： Wen-hui fang Yong-Ming yao Zhi-Guo Shi Yan Yu Ye Wu Lian-Rong Lu Zhi-Yong Sheng Department of Microbiology and Immunology,Burns Institute,304th Hospital of PLA,Beijing 100037,China Hosp PLA 304th Burns Inst Dept Microbiol & Immunol Beijing 100037 Peoples R China

AIM: To investigate changes of tumor necrosis factor-α (TNFα) and TNFR-Ⅰ expression in vital organs and their significance in the pathogenesis of multiple organ damage associated with endogenous endotoxin following major ***: Wistar rats subjected to a 35 % full-thickness scald injury were sacrificed at 12 h, 24 h, 48 h, and 72 h postburn, respectively. Meanwhile, eight rats were taken as normal controls. Tissue samples from liver, spleen, kidney,lung and intestine were collected to assay tissue endotoxin levels and measure TNF-α and TNFR-Ⅰ expression, In addition, blood samples were obtained for the determination of organ function ***: Endotoxin levels in liver, spleen and lung increased markedly after thermal injury, with the highest level in liver. The gene expression of TNF-α in liver, lung and kidney was up-regulated after thermal injury, while the TNFR-Ⅰ mRNA expression in liver, lung, kidney and intestine was shown decreased throughout the observation period. Thus, the mRNA expression ratio of TNF-α to TNFRⅠ was significantly increased postburn, particularly in pulmonary tissue (67-fold). In addition, the significant correlations between the expression of TNFR-Ⅰ or the expression ratio of TNF-α/TNFR mRNA in liver tissue and serum aspartate aminotransferase levels were noted (P ＜0.05-0.01). Similar results were also obtained between pulmonary TNF-α mRNA expression and myeloperoxidase activities (P＜0.01), whereas there was a highly negative correlation between levels of renal TNFR-Ⅰ mRNA expression and serum ***: Burn injury could result in the translocation of gut-derived endotoxin that was mainly distributed in the liver, spleen and lung. The translocated endotoxin then made the expression of TNF-α and TNFR-Ⅰ mRNA up-regulated and down-regulated respectively in various organs, which might be involved in the pathogenesis of multiple organ damage following burns.

关键词：肿瘤坏死因子-α 受体热损伤 mRNA 烧伤细胞因子炎症动物实验

热毒宁注射液化学成分、药理作用及临床应用研究进展

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中草药 2017年第5期48卷 1027-1036页

作者：葛雯李海波于洋房卉姚新生南京中医药大学江苏南京210046 暨南大学中药及天然药物研究所广东广州510632

热毒宁注射液具有清热、疏风、解毒的功效,临床上主要用于治疗外感风热所致的感冒、咳嗽及上呼吸道感染、急性支气管炎等,其临床疗效确切,安全性好,应用广泛。热毒宁注射液的化学成分主要包括环烯醚萜类、木脂素类、香豆素类、倍半萜类... 详细信息

热毒宁注射液具有清热、疏风、解毒的功效,临床上主要用于治疗外感风热所致的感冒、咳嗽及上呼吸道感染、急性支气管炎等,其临床疗效确切,安全性好,应用广泛。热毒宁注射液的化学成分主要包括环烯醚萜类、木脂素类、香豆素类、倍半萜类、黄酮类、咖啡酰奎宁酸类、酚酸类等。通过查阅近几年与热毒宁注射液相关的国内外文献,对其化学成分、药理作用及临床应用进行综述。

关键词：热毒宁注射液环烯醚萜类木脂素类香豆素类倍半萜类黄酮类咖啡酰奎宁酸类解热抗炎抗病毒

Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

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World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： Juan fang Li-hui Li Ming-Qian He Yue Ji Dong-Yan Lu Li-Bing Zhang Jin-Lan yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

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Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing Zhang Xue fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

Burden of mental disorders and risk factors in the Western Pacific region from 1990 to 2021

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World Journal of Psychiatry 2025年第1期15卷 93-105页

作者： Ya-Xin Xu Xiao-Xuan Niu Wen-Chang Jia Jing Wen Xue-Lin Cheng Yan Han Ming-hui Peng Jing Zhou yao Liu Sun-fang Jiang Xiao-Pan Li Department of Health Management Centre Zhongshan HospitalFudan UniversityShanghai 200030China Department of General Practice Zhongshan HospitalFudan UniversityShanghai 200030China Department of Nutrition Zhongshan HospitalFudan UniversityShanghai 200032China School of Public Health Fudan UniversityShanghai 200032China

BACKGROUND The burden of mental disorders(MD)in the Western Pacific Region(WPR)re-mains a critical public health concern,with substantial variations across demogra-phics and *** To analyze the burden of MD in the WPR from 1990 to 2021,along with associated risk factors,to reveal changing trends and emerging *** We used data from the Global Burden of Disease 2021,analyzing prevalence,incidence,and disability-adjusted life years(DALYs)of MD from 1990 to *** methods included age-standardisation and uncertainty analysis to address variations in population structure and data *** Between 1990 and 2021,the prevalence of MD rose from 174.40 million cases[95%uncertainty interval(UI):160.17-189.84]to 234.90 million cases(95%UI:219.04-252.50),with corresponding DALYs increasing from 22.8 million(95%UI:17.22-28.79)to 32.07 million(95%UI:24.50-40.68).During this period,the burden of MD shifted towards older age *** and anxiety disorders were predominant,with females showing higher DALYs for depressive and anxiety disorders,and males more affected by conduct disorders,attention-deficit hyperactivity disorder,and autism spectrum ***,New Zealand,and Malaysia reported the highest burdens,whereas Vietnam,China,and Brunei Darussalam reported the ***,childhood sexual abuse and bullying,and intimate partner violence emerged as significant risk *** This study highlights the significant burden of MD in the WPR,with variations by age,gender,and *** coronavirus disease 2019 pandemic has exacerbated the situation,emphasizing the need for a coordinated response.

关键词： Mental disorders Western pacific region Global Burden of Disease Risk factors Disability-adjusted life years

遮阴条件下大叶竹节树光合特性和荧光参数的变化

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西南大学学报（自然科学版） 2018年第3期40卷 68-74页

作者：黄菁魏丽萍孙小龙原慧芳田耀华云南省热带作物科学研究所云南景洪666100

为研究大叶竹节树对弱光的适应性,通过盆栽实验,分析了不同程度(0%,25%,50%,75%)遮阴条件下大叶竹节树幼苗光合特性和荧光参数的变化.结果表明,随着遮阴程度增加,叶片净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、水分利用效率(WUE)和... 详细信息

为研究大叶竹节树对弱光的适应性,通过盆栽实验,分析了不同程度(0%,25%,50%,75%)遮阴条件下大叶竹节树幼苗光合特性和荧光参数的变化.结果表明,随着遮阴程度增加,叶片净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr)、水分利用效率(WUE)和光合有效辐射(PAR)随之减小,而胞间CO2浓度(Ci)随之增大,说明非气孔制限因素是大叶竹节树光合速率下降的主要原因.各遮阴条件下叶片总叶绿素(a+b)、叶绿素a和叶绿素b质量比均显著高于全光照,且随遮阴程度的增加叶绿素质量比逐渐上升,而叶绿素a/b值则随着遮阴程度的增强而下降.叶绿素荧光参数PSⅡ原初光能转化效率(Fv/Fm)和潜在活性(Fv/Fo)的日变化呈单谷曲线;各遮阴条件下Fv/Fm和Fv/Fo值均高于全光照,且与遮阴程度正相关,说明大叶竹节树是一种耐荫性很强的植物.在遮阴条件下,光补偿点、光饱和点、净光合速率、暗呼吸速率和叶绿素a/b值降低,而叶绿素a和b质量比、光能利用率、Fv/Fm以及Fv/Fo值提高,从而增强弱光条件下的生长能力.

关键词：大叶竹节树遮阴处理光合特性荧光参数

An Efficient Boron Source Activation Strategy for the Low‑Temperature Synthesis of Boron Nitride Nanotubes

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Nano-Micro Letters 2025年第1期17卷 548-558页

作者： Ying Wang Kai Zhang Liping Ding Liyun Wu Songfeng E Qian He Nanyang Wang hui Zuo Zhengyang Zhou Feng Ding Yue Hu Jin Zhang Yagang yao National Laboratory of Solid State Microstructures College of Engineering and Applied SciencesJiangsu Key Laboratory of Artificial Functional Materialsand Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093People’s Republic of China School of Electronic Information and Artificial Intelligence Shaanxi University of Science&TechnologyXian 710000People’s Republic of China College of Bioresources Chemical and Materials Engineering Shaanxi University of Science&TechnologyXian 710000People’s Republic of China Key Laboratory of Carbon Materials of Zhejiang Province College of Chemistry and Materials EngineeringWenzhou UniversityWenzhou 325000People’s Republic of China Shenzhen Institutes of Advanced Technology Chinese Academy of SciencesShenzhen 518000People’s Republic of China College of Chemical and Molecular Engineering Peking UniversityBeijing 100871People’s Republic of China

Lowering the synthesis temperature of boron nitride nanotubes(BNNTs)is crucial for their *** primary reason for adopting a high temperature is to enable the effective activation of highmelting-point solid *** this study,we developed a novel approach for efficiently activating boron by introducing alkali metal compounds into the conventional MgO–B *** approach can be adopted to form various low-melting-point AM–Mg–B–O growth *** growth systems have improved catalytic capability and reactivity even under low-temperature conditions,facilitating the synthesis of BNNTs at temperatures as low as 850℃.In addition,molecular dynamics simulations based on density functional theory theoretically demonstrate that the systems maintain a liquid state at low temperatures and interact with N atoms to form BN *** findings offer novel insights into the design of boron activation and are expected to facilitate research on the low-temperature synthesis of BNNTs.

关键词： Boron nitride nanotubes Low-temperature Boron activation Density functional theory

Uniqueness on Difference Operators of Meromorphic Functions of Infinite Order

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Acta Mathematica Sinica,English Series 2024年第2期40卷 511-527页

作者： hui LI Ming Liang fang Xiao yao School of Science China University of Mining and TechnologyBeijing 100083P.R.China Institute of Mathematics Academy of Mathematics and System SciencesChinese Academy of SciencesBeijing 100190P.R.China School of Sciences Hangzhou Dianzi UniversityHangzhou 310012P.R.China School of Mathematical sciences and LPMC Nankai UniversityTianjin 300071P.R.China

We investigate the uniqueness problems of meromorphic functions and their difference operators by using a new *** is proved that if a non-constant meromorphic function f shares a non-zero constant and ∞ counting multiplicities with its difference operators Δcf(z) and Δ_(c)^(2)f(z), thenΔcf(z)≡Δ_(c)^(2)f(z).In particular,we give a difference analogue of a result of *** method has two distinct features:(ⅰ) It converts the relations between functions into the corresponding *** makes it possible to deal with the uniqueness problem by linear algebra and combinatorics.(ⅱ) It circumvents the obstacle of the difference logarithmic derivative lemma for meromorphic functions of infinite order,since this method does not depend on the growth of the ***,the idea in this paper can also be applied to the case for several variables.

关键词： Uniqueness meromorphic functions difference operators infinite order