检索结果-南通市图书馆

Immune Blot Analysis on Expression of the mammalian Target of Rapamycin in Goat Fetal Fibroblasts with Recombinant Polyclonal Antibody

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2012年第6期11卷 1002-1008页

作者： LIANG Yan LI Shu-yu WANG Xiao-jing WU man-lin YANG Jiao-fu LI Jie HAO Xi-yan HAO hui-fang BA Yin-ma CHEN Xian-wei WANG Zhi-gang College of Life Science lnner Mongolia UniversityHohhot 010021P.R.China

To detect the mammalian target of rapamycin （mTOR） expressed in Cashmere goat fetal fibroblasts （GFb）, mTOR gene was cloned from Inner Mongolia Cashmere goat （Capra hircus） and expressed in Escherichia coli followed by immunizing mice with the purified recombinant protein as an irnmunogen to produce the anti-goat mTOR recombinant polyclonal antibody. Antiserum was collected from the immunized mice after the fifth immunization and its titer was determined with enzyme-linked immunosorbent assay （ELISA）. The results showed that the recombinant polyclonal antibody had a titer 1：200000 and could react with the roTOR expressed in GFb cells with a specific and sensitive affinity. Western blot showed that mTOR expression and phospho-mTOR （Ser 2448） activity were inhibited when GFb cells were treated with CCI-779, an mTOR specific inhibitor.

关键词： prokaryotic expression recombinant antibody goat mTOR CCI-779

A 3D Luminescent Cd(Ⅱ) Compound Constructed from Succinate and 3,6-Di(pyridyl)pyridazine

在线全文

学校读者我要写书评

暂无评论

A 3D Luminescent Cd(Ⅱ) Compound Constructed from Succinate ...

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

中国化学会第七届全国结构化学学术会议

作者： hui-fang ma Lin-Na Xiong Yu-Ling Wang Qing-Yan Liu College of Chemistry and Chemical Engineering Jiangxi Normal University

A new coordination polymer[Cd(SC)(DPPD)](1,HSC=succinic acid and DPPD=3,6-di(4-pyridyl)pyridazine)has been ***-crystal X-ray analysis reveals that the compound 1 crystallizes in the monoclinic system,space group P2/*** compound 1,the Cd(Ⅱ)ions are linked by the SCligands to give a 2D undulating sheet based on the centrosymmetric

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun Nie Jing Zhang Xue fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

Hypidone hydrochloride(YL-0919)ameliorates functional deficits after traumatic brain injury in mice by activating the sigma-1 receptor for antioxidation

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第8期20卷 2325-2336页

作者： Yafan Bai hui ma Yue Zhang Jinfeng Li Xiaojuan Hou Yixin Yang Guyan Wang Yunfeng Li Department of Anesthesiology Beijing Tongren HospitalCapital Medical UniversityBeijingChina Beijing Institute of Basic Medical Sciences BeijingChina Hebei North University ZhangjiakouHebei ProvinceChina Beijing Institute of Pharmacology and Toxicology State Key Laboratory of Toxicology and Medical CountermeasuresBeijing Key Laboratory of NeuropsychopharmacologyBeijingChina

Traumatic brain injury involves complex pathophysiological mechanisms,among which oxidative stress significantly contributes to the occurrence of secondary *** this study,we evaluated hypidone hydrochloride(YL-0919),a self-developed antidepressant with selective sigma-1 receptor agonist properties,and its associated mechanisms and targets in traumatic brain *** experiments to assess functional deficits were followed by assessment of neuronal damage through histological analyses and examination of blood-brain barrier permeability and brain ***,we investigated the antioxidative effects of YL-0919 by assessing the levels of traditional markers of oxidative stress in vivo in mice and in vitro in HT22 ***,the targeted action of YL-0919 was verified by employing a sigma-1 receptor antagonist(BD-1047).Our findings demonstrated that YL-0919 markedly improved deficits in motor function and spatial cognition on day 3 post traumatic brain injury,while also decreasing neuronal mortality and reversing blood-brain barrier disruption and brain ***,YL-0919 effectively combated oxidative stress both in vivo and in *** protective effects of YL-0919 were partially inhibited by *** results indicated that YL-0919 relieved impairments in motor and spatial cognition by restraining oxidative stress,a neuroprotective effect that was partially reversed by the sigma-1 receptor antagonist ***-0919 may have potential as a new treatment for traumatic brain injury.

关键词： antidepressant drug blood-brain barrier cognitive function hypidone hydrochloride(YL-0919) neurological function nuclear factor-erythroid 2 related factor 2 oxidative stress sigma-1 receptor superoxide dismutase traumatic brain injury

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li hui-Min Xing Hai-Dong Qian Qiao Gao Sheng-Lan Xu Hua ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

在线全文

学校读者我要写书评

暂无评论

针灸治疗慢性胃炎临床方法对比分析

针刺研究 2004年第4期29卷 310-313页

作者：马惠芳马文珠郭长青北京中医药大学针灸学院北京100029

本文就近 1 0年来针灸治疗慢性胃炎概况进行了综合分析与归纳 ,并对针灸治疗本病的疗效与安全性评价、针灸治疗规律及针灸临床研究重点进行了分析 ,以期为临床更好地治疗慢性胃炎提供参考资料。

关键词：慢性胃炎针灸疗法综合疗法安全性

Application of Dorzagliatin in peritoneal dialysis patients with type 2 diabetes mellitus:A case report

在线全文

学校读者我要写书评

暂无评论

World Journal of Diabetes 2025年第1期16卷 204-210页

作者： fang Chen Bo An Wen-Cheng An Gang Fu Wei Huang hui-Xian Yan Department of Endocrinology Beijing Haidian HospitalBeijing 100080China Department of Nephrology Beijing Haidian HospitalBeijing Haidian Section of Peking University Third HospitalBeijing 100080China

BACKGROUND Treating diabetes in dialysis patients remains a challenge,with many hypoglycemic drugs requiring dose adjustments or avoidance in these *** SUMmaRY This report describes an 83-year-old female patient with a 30-year history of type 2 diabetes(T2DM)who had struggled to control her blood sugar for more than a *** had a history of high blood pressure for 30 years,had undergone continuous ambulatory peritoneal dialysis for more than two years,was 163 cm tall,weighed 77 kg,and had a body mass index of 28.98 kg/*** intensive insulin therapy at a daily dose of 150 units,adding Dorzagliatin at a dosage of 75 mg orally twice daily led to immediate blood sugar improvement and a gradual reduction in insulin *** one month of follow-up,the fasting plasma glucose was 6-8 mmol/L,and the 2-hour postprandial glucose was 8-12 mmol/*** To our knowledge,this report is the first to use Dorzagliatin to treat type 2 diabetes peritoneal dialysis patients with challenging glucose ***,a novel glucokinase activator primarily metabolized by the liver,exhibits no pharmacokinetic differences among patients with varying degrees of chronic kidney *** has a high plasma protein binding rate and may not be cleared by peritoneal dialysis,potentially offering a new glycemic control option for Type 2 diabetic patients on peritoneal dialysis.

关键词： Dorzagliatin Type 2 diabetes mellitus Peritoneal dialysis Glucokinase activator Glucose control Case report

垄断黑金与暴力魅影——王兴强黑社会性质组织犯罪评析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

西南政法大学学报 2010年第2期12卷 121-129页

作者：任惠华马方西南政法大学

公安部挂牌督办的王兴强黑社会性质组织为追逐非法利润,以合法企业为外衣,暴力垄断市场;该黑社会性质组织结构严密,并拉拢国家工作人员包庇、纵容犯罪行为。在司法实践中,应加强市场监管,严厉整治社会秩序;制定专门法规,准确认定黑社会... 详细信息

公安部挂牌督办的王兴强黑社会性质组织为追逐非法利润,以合法企业为外衣,暴力垄断市场;该黑社会性质组织结构严密,并拉拢国家工作人员包庇、纵容犯罪行为。在司法实践中,应加强市场监管,严厉整治社会秩序;制定专门法规,准确认定黑社会性质组织犯罪;强化司法部门的特殊打击能力,有力打击黑社会性质组织犯罪;严厉打击国家工作人员涉黑职务犯罪,彻底消除"保护伞"的庇护;加大对洗钱行为的预防、监控和打击力度。

关键词：王兴强黑社会性质组织犯罪垄断市场暴力手段

Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： Juan fang Li-hui Li Ming-Qian He Yue Ji Dong-Yan Lu Li-Bing Zhang Jin-Lan Yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation