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Procedure for preparing peptide-major histocompatibility complex tetramers for direct quantification of antigen-specific cytotoxic T lymphocytes

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World Journal of Gastroenterology 2005年第27期11卷 4180-4187页

作者： Xian-hui He Li-hui Xu Yi Liu Key Laboratory of Ministry of Education of c.ina for Tissue Transplantation and immunology Jinan UniversityGuangzhou 510632 Guangdong Province China institute of Bioengineering Jinan University Guangzhou 510632 Guangdong Province China Department of Dermatology First Affiliated HospitalZhengzhou University Zhengzhou 450052 Henan Province China

AiM： To establish a simplified method for generating peptide-major histocompatibility complex （MHc） class i ***： cDNAs encoding the extracellular domain of human lymphocyte antigen （HLA）-A＊0201 heavy chain （A2） and β2-microglobulin （132m） from total RNA extracted from leukocytes of HLA-A2＋ donors were doned into separate expression vectors by reverse transc.iption-polymerase chain reac.ion. The recombinant A2 and 132m proteins were expressed in ~/a oo/i^uain BL21（DE3） and recovered from the inclusion body frac.ion. Soluble A2 proteins loaded with specific antigen peptides were refolded by dilution from the heavy chain in the presence of light chain 132m and HLA-A2-restricted peptide antigens. The refolded A2 monomers were biotinylated with a commercial biotinylation enzyme （BirA） and purified by low pressure anion exchange chromatography on a Q-Sepharose （fast flow） *** tetramers were then formed by mixing A2 monomers with streptavidin-PE in a molar ratio of 4：1. Flow cytometry was used to confirm the expected tetramer staining of cD8^＋ T ***： Recombinant genes for HLA-A＊0201 heavy chain （A2） fused to a BirA substrate peptide （A2-BSP） and mature β2m from HLA-A2＋ donor leukocytes were successfully doned and highly expressed in E. coli, Two soluble monomeric A2-peptide complexes were reconstituted from A2-BSP in the presence of 132m and peptides loaded with either human cytomegalovirus pp65495-503 peptide （NLVPMVATV,NLV; designated as A2-NLV） or influenza virus matrix protein Mp58-66 peptide （GiLGFVFTL, GiL; designated as A2-GiL）. Refolded A2-NLV or A2-GiL monomers were biotinylated and highly purified by single step anion exchange column chromatography. The tetramers were then formed by mixing the biotinylated A2-NLV or A2-GiL monomers with streptavidin-PE, leading to more than 80% multiplicationas revealed by SDS-PAGE under non-reducing, unboiled conditions. Flow cytometry revealed that these tetramers could specifically bind to cD8^＋

关键词： cloning, Molecular HLA-A Antigens HLA-A2 Antigen Humans Recombinant Fusion Proteins Research Support, Non-U.S. Gov't T-Lymphocytes, cytotoxic beta 2-Microglobulin

Synthesis,Spectroscopic Properties and Structural characterization of Sodium Potassium citrato Oxotungstate(Vi)Dimer

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c.inese Journal of Structural chemistry 2000年第3期19卷 181-186页

作者： WANG Geng ZHOU Zhao-hui ZHANG hui YE Jian-Liang WAN hui-Lin Department of chemistry and State Key Laboratory for Physical chemistry of Solid Surface Xiamen UniversityXiamen361005

The sodium potassium citrato oxotungstate(Vi) dimer, Na 4K 2〔W 2O 5\|(cit) 2〕·11H 2O (H 4cit = citric acid) was obtained by the reac.ion of sodium tungstate(Vi) and excess potassium citrate monobasic (KH 3cit) in neutral solution. The crystal data for the title compound: monoc.inic, c 12 H 30 K 2Na 4W 2O 30 , M r =1192.21, space group P2 1/n, a=17.427(4), b=10.022(2), c=18.637(3) , β=92.62(1)°, V=3252(2)  3, Z=4, D c =2.435 g/cm 3, μ( Mo Kα )=76.38 cm -1 , F( 000)=2288. The structure was refined to R =0.0434 for 6327 independent observed reflec.ions with i>3σ(i) . The complex anion contains a quasi centrosymmetric (O 2W)O b(WO 2) core with a bent bridging oxo group 〔W-O b-W 175.0(4)°〕. Each citrate as tridentate ligand coordinates to a tungsten atom through the alkoxy, α carboxyl, and one β carboxyl group, while the other β carboxyl group remains uncoordinated. Principal dimensions are: W-O b 1.883(6) , (W=O t)av 1.715(6) , W-O (alkoxy) 1.959(6) , W-O ( α carboxy) 2.176(6)  and W-O ( β carboxy) 2.231(6) . The tungsten atom is located in a distorted octahedron environment.

关键词： oxotungstate(Vi) citrate citric acid citrate method crystal structure

A new sesquiterpene from hairy root culture of Artemisia annua

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c.inese chemical Letters 2010年第5期21卷 590-592页

作者： Dan Dan Zhai[a], hui Zi Jin[b] Jian Jiang Zhong[a][c] [a] State Key Laboratory of Bioreactor Engineering School of Biotechnology East China University of Science Technology Shanghai 200237 China [b] School of Pharmacy Shanghai Jiaotong University Shanghai 200240 China [c] Key Laboratory of Microbial Metabolism Ministry of Education School of Life Sciences & Biotechnology Shanghai Jiaotong University Shanghai 200240 China

A new sesquiterpene（Z）-7-acetoxy-methyl-11-methyl-3-methylenedodeca-1,6,10-triene（AMDT） was isolnted and identified from the methanol extract of the hairy root culture of Artemisia *** structure of AMDT was determined based on the analysis of spectroscopic data,notably of the 2D NMR *** new compound showed cytotoxicity against human tumor cell lines 95-D and HeLa with ic50 values of 27.08 and 20.12μmol/L,respec.ively.

关键词： Artemisia annua Sesquiterpene cytotoxicity

Asteroseismology of the DBV star cBS 114

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Research in Astronomy and Astrophysics 2016年第8期16卷 17-24页

作者： Yan-hui chen institute of Astrophysics Chuxiong Normal University School of Physics and Electronical Science Chuxiong Normal University Key Laboratory for the Structure and Evolution of celestial Objects Chinese Academy of Sciences

Asteroseismology is a unique and powerful tool to investigate the internal structure of stars. cBS 114 is the sixth known pulsating DBV star. it was observed by Handler, Metcalfe, ＆ Wood at the South African Astronomical Observatory over three weeks in 2001. Then, it was observed by Metcalfe et al. for seven nights （2004 Feb. 19-25） on the 1.8 m telescope at the Bohyunsan Optical Astronomy Observatory and seven nights （2004 Feb. 21-27） on the 2.1 m telescope at the McDonald Observatory. Totally two triplets, four doublets, and five singlets were identified. The frequency splitting values are very different, from 5.2 *** to 11.9 ~tHz, which may reflect differential rotations. We evolve grids of white dwarf models by MESA. cores, added with He/c envelopes, of those white dwarf models are inserted into WDEc to evolve grids of DBV star models. With those DBV star models, we calculate eigenperiods. Those calculated periods are used to fit observed periods. A best-fitting model is selected. The parameters are T^ff ----- 25 000 K, M, = 0.740 MG and log（MHe/M,） = -4.5. With the relatively large stellar mass, the effec.ive temperature is close to the previous spectroscopic result. in addition, kinetic energy distributions are calculated for the best-fitting model. We find that the observed modes with large frequency splitting values are fitted by the calculated modes with a large amount of kinetic energy distributed in the c/O core. After preliminary analysis, we suggest that the c/O core may rotate at least two times faster than the helium layer for cBS 114.

关键词： stars: oscillations (including pulsations) -- stars: individual (cBS 114) -- white dwarfs

Effects of grazing on carbon and nitrogen in plants and soils in a semiarid desert grassland,c.ina

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Journal of Arid Land 2015年第3期7卷 341-349页

作者： hui AN GuoQi Li Ministry of Education Key Laboratory for Restoration and Reconstruc.ion of Degraded Ecosystem in Northwestern c.ina United Center for Ecology Research and Bioresource Exploitation in Western ChinaNingxia University

Grazing can modulate the feedback between vegetation and soil nutrient dynamics（carbon and nitrogen）,altering the cycles of these elements in grassland *** clarifying the impact of grazing on the c and N in plants and soils in the desert grassland of Ningxia,c.ina,we examined the plant biomass,SOc（soil organic carbon）,total soil N and stable isotope signatures of plants and soils from both the grazed and ungrazed *** lower aboveground biomass,root biomass,litter biomass and vegetation coverage were found in the grazed site compared to the ungrazed site,with decreases of 42.0%,16.2%,59.4% and 30.0%,*** effects of grazing on plant carbon,nitrogen,？15N and ？13c values were uniform among *** levels of plant carbon and nitrogen in grasses were greater than those in the forbs（except for the carbon of cynanchum komarovii and Euphorbia esula）.Root 15 N and 13 c values increased with grazing,while the responses of root carbon and nitrogen to grazing showed no consistent *** 15 N and 13 c were increased by 79.0% and 22.4% in the grazed site compared to the ungrazed site,*** values of SOc and total N were significantly lower in the grazed than in the ungrazed sites for all sampling depths（0–10 and 10–20 cm）,and values of SOc and total N at the surface（0–10 cm） were lower than those in the deeper soils（10–20 cm）.Soil ？15N values were not affected by grazing at any sampling depth,whereas soil ？13c values were significantly affected by grazing and increased by 19.3% and 8.6% in the soils at 0–10 and 10–20 cm,*** soil ？13c values（–8.3‰ to –6.7‰） were higher than those for roots（–20.2‰ to –15.6‰） and plant tissues（–27.9‰ to –13.3‰）.Our study suggests that grazing could greatly affect soil organic carbon and nitrogen in contrast to ungrazed grassland and that grazing appears to exert a negative effect on soil carbon and nitrogen in desert grassland.

关键词： grazing 15N 13c soil properties desert grassland plant properties

Two Stochastic Resonances induced by Two Different Multiplicative Telegraphic Noises for an Electric System

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communications in Theoretical Physics 2008年第11期50卷 1159-1164页

作者： Li Jing-hui Faculty of Science P.O. Box 58 Ningbo University Ningbo 315211 China

in this paper,an electric system with two dichotomous resistors is *** is shown that thissystem can display two stochastic resonances,which are the amplitude of the periodic response as the func.ions of thetwo dichotomous resistors strengthes *** the limits of Gaussian white noise and shot white noise(i.e.,thetwo noises are both Gaussian white noise or shot white noise),no phenomena of resonance *** further study,we find that when the system is with three or more multiplicative telegraphic noises,there are three or more stochasticresonances.

关键词： stochastic resonance noise

Status monitoring and health assessment of Luhuitou fringing reef of Sanya, Hainan, c.ina

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c.inese Science Bulletin 2006年第B12期51卷 81-88页

作者： ZHANG Qiaomin SHi Qi cHEN Gang FONG T c W WONG D c c HUANG hui WANG Hankui ZHAO Meixia South c.ina Sea institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China Kay Laboratory of Marginal Sea Geology Chinese Aeademy of Sciences Guangzhou 510301 China Department of Marine and Fishery of Hainan Province Haikou 570203 China Environmental Resources Management Hong Kong China Hong Kong cetacean Research Project Hong Kong China Tropical Marine Biological Research Station in Halnan Chinese Academy of Sciences Sanya 572000 China

The monitoring survey and health assessment for benthic community were carried out in Luhuitou fringing reef of Sanya, Hainan, c.ina in 2002. Monitoring survey included manta tow, line intercept transect, video transect and digital photograph. The mean live coral cover was 23.40%. comparison of used monitoring methods showed that video transect can replace line intercept transect, and 50 m transect is better than 20 m transect in uneven coral region. Survey data and comparison with previous research work showed that Luhuitou fringing reef has been damaged severely and has a general dec.ining trend with live coral cover from about 85% in the 1960s down to 20% in 2004, but it still has quite high biodiversity and therefore has special protec.ion value. Main factors of Luhuitou fringing reef dec.ining were human ac.ivities including reef rock digging and curios collec.ing, destruc.ive fishing, sediment and pollutant from coastal land, and recent marine aquaculture. Only strengthening integrated management can reach improvement and restoration of Luhuitou dec.ining coral reef ecosystem.

关键词：海南岛三亚鹿回头珊瑚礁生物多样性环境监测健康评估

Zero Dissipation Limit to Rarefac.ion Waves for the p-System

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Acta Mathematica Sinica,English Series 2005年第5期21卷 1229-1240页

作者： hui Ying WANG center of Mathematical Science Zhejiang University Hangzhou 310027 P. R. China

We study the zero-dissipation problem for a one-dimensional model system for the isentropic flow of a compressible viscous gas, the so-called p-system with viscosity. When the solution of the inviscid problem is a rarefac.ion wave with finite strength, there exists unique solution to the viscous problem with the same initial data which converges to the given inviscid solution as c goes to zero. The proof consists of a scaling argument and elementary energy analysis, based on the underlying wave structure.

关键词： Zero dissipation problem p-system centered rarefac.ion wave

Stochastic Resonance of Single (independent of Each Other) Protein Motor System with Fluctuating Potential Barrier

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communications in Theoretical Physics 2008年第4期49卷 945-950页

作者： Li Jing-hui P.O. Box 58 Faculty of Science Ningbo University Ningbo 315211 China

A single （independent of each other） protein motor system with fluctuating potential barrier and subject to sine electric field is investigated. We first derive the approximate Langevin equation of this system with fluctuating potential barrier. Then from this approximate Langevin equation, we calculate the signal-to-noise ratio （SNR） in the adiabatic limit. The phenomenon of stochastic resonance is found for this protein motor system with fluctuating potential barrier.

关键词： stochastic resonance protein motor fluctuating potential barrier