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Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines

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World Journal of Gastroenterology 2012年第31期18卷 4162-4168页

作者： Raimundo Fernandes de Araújo Júnior Luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino hugo gon alo guedes Pedro Ros Petrovick Tatiane Pereira de Souza Aurigena Antunes de Araújo Gerlane Coelho Bernardo Guerra Department of Morphology/Postgraduate Program in Morphological Sciences Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of PernambucoRecife 50670-901PernambucoBrazil Postgraduate Program in Pharmaceutical Sciences Federal University of Rio Grande do NorteNatal 59012-570Rio Grande do NorteBrazil Department of Production and control of drugs Federal University of Rio Grande do SulPorto Alegre 90610-000Rio Grande do SulBrazil Department of Food and Drug Administration Federal University of AmazonasManaus 69077-000AmazonasBrazil Department of Biophysics and Pharmacology Federal University of Rio Grande do NorteNatal 59072-970Rio Grande do NorteBrazil

AIM:To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell ***:Colorectal carcinoma(HT29) and human hepatocellular carcinoma(HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri(SDEPN) either alone or in combination with cisplatin at different concentrations(0.5 mg/mL and 1 mg/mL) for 4 h and 24 *** verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability,we stained the cells with propidium iodide and assessed them by flow *** percentage of cells in different cell cycle phases was quantified and data were expressed as *** differences between groups were determined using analysis of variance and Bonferroni's test,as indicated.A value of P 0.05) and HepG2(5.07 ± 0.3 vs 15.9 ± 1.04,P 0.05) and HepG2(14.56 ± 1.6 vs 35.67 ± 3.94,P < 0.001) cells when compared to control cells for 24 *** cell lines were killed by cisplatin in a dose-dependent manner compared to control cells(HepG2 cells for 4 h:10.78 ± 1.58 vs 53.89 ± 1.53,P < 0.001;24 h:8.9 ± 1.43 vs 62.78 ± 1.87,P < 0.001 and HT29 cells for 4 h:9.52 ± 0.913 vs 49.86 ± 2.89,P < 0.001;24 h:11.78 ± 1.05 vs 53.34 ± 2.65,P < 0.001).In HT29 cells,pretreatment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed(12.78 ± 1.01 vs 93.76 ± 1.6,P < 0.001).HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin(12.87 ± 2.78 vs 78.8 ± 3.02,P < 0.001).CONCLUSION:SDEPN is selectively toxic against two cancer cell ***,SDEPN in combinati

关键词：肿瘤细胞株提取物生长抑制作用顺铂珍珠 HepG2细胞细胞毒作用喷雾干燥

Percutaneous nephrostomy versus ureteral stent in hydronephrosis secondary to obstructive urolithiasis:A systematic review and meta-analysis

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Asian Journal of Urology 2024年第2期11卷 261-270页

作者： Andreia Cardoso Aparício Coutinho gonçalo Neto Sara Anacleto Catarina Laranjo Tinoco Nuno Morais Mário Cerqueira-Alves Estevão Lima Paulo Mota Department of Urology Hospital de BragaBragaPortugal School of Medicine University of MinhoBragaPortugal Association(2CA-Braga).School of Medicine University of MinhoBragaPortugal

Objective: To assess if there is a preferable intervention between retrograde ureteral stent (RUS) and percutaneous nephrostomy (PCN) tube, in cases of upper urinary tract stone obstruction with complications requiring urgent drainage, by evaluating outcomes regarding urinary symptoms, quality of life (QoL), spontaneous stone passage, and length of hospital stays, since there is no literature stating the superiority of one modality over the ***: We searched MEDLINE and other sources for relevant articles in June 2019 without any date restrictions or filters applied. The selection was done first by the title and abstract screening and then by full-text assessment for eligibility. Only randomized controlled trials or cohort studies in patients with hydronephrosis secondary to obstructive urolithiasis that presented comparative data between PCN and RUS placement concerning at least one of the defined outcome measures were included. Lastly, MEDLINE database and PubMed platform were screened again using the same terms, from June 2019 until November ***: Of 556 initial articles, seven were included in this review. Most works were considered of moderate-to-high quality. Three studies regarding QoL showed a tendency against stenting, even though only one demonstrated statistically significant negative impact on overall health state. Two works reported significantly more post-intervention urinary symptoms in stenting patients. One article found that PCN is a significant predictor of spontaneous stone passage, when adjusted for stone size and location. Findings on length of hospital stays were not consistent among ***: PCN appears to be the intervention better tolerated, with less impact on the patient’s perceived QoL and less post-operative urinary symptoms, in comparison with RUS. Nevertheless, further studies with larger samples and a randomized controlled design are suggested.

关键词： Ureteral stent Percutaneous nephrostomy Obstructive urolithiasis Urinary symptoms Quality of life

Conflicts in passive building performance:Retrofit and regulation of informal neighbourhoods

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Frontiers of Architectural Research 2021年第3期10卷 625-638页

作者： gonçalo Araújo Inês Pereira António Leitão Manuel Correia guedes INESC-ID/Instituto Superior Tećcnico University of LisbonLisbonPortugal Instituto Superior Tećcnico University of LisbonLisbonPortugal

Urbanization growth in developing countries raises concerns regarding these countries’ability to consider slums,underdeveloped communities,and neighbourhoods in economic,health,and climatic *** research proposes a methodology that integrates algorithmic design and analysis strategies to define,study,and measure key parameters that affect the rehabilitation of these *** scenarios and design dimensions are analysed to establish design and comfort thresholds,and alternatives are simulated and tested to identify possible *** methodology includes an optimisation step integrated in the workflow that maximizes thermal comfort,minimizes costs,and ensures fairness in the rehabilitation of large sets of *** step identifies improvements in thermal comfort for different construction scenarios from which a two-staged rehabilitation plan is *** first stage comprises a sensitivity analysis to identify building materials regarding their improvement and cost of application,and the second defines the most suitable construction scenarios considering the results from the optimisation process for each ***,we research and document guidelines regarding the parameters tested for building design,revealing the existing conflicts between performance objectives,and the architect’s role in their prioritization.

关键词： Urban regeneration Informal housing Algorithmic design Optimisation Simulation

Mesenchymal stem cell-and extracellular vesicle-based therapies for Alzheimer's disease:progress,advantages,and challenges

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Neural Regeneration Research 2023年第8期18卷 1645-1651页

作者： Renata guedes de Jesus gonçalves Juliana Ferreira Vasques Almir Jordão da Silva-Junior Fernanda Gubert Rosalia Mendez-Otero Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa Rio de JaneiroBrazil Plataformas Inovadoras em Terapias Avançadas Departamento de Ciência e Tecnologia-Ministério da Saúde(DECIT-MS)Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)Brazil Programa Redes de Pesquisa em Saúde no Estado do Rio de Janeiro Rio de JaneiroBrazil Instituto de Ciências Biomédicas Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Programa Redes de Pesquisa em Nanotecnologia no Estado do Rio de Janeiro Rio de JaneiroBrazil

Alzheimer's disease is a severe, highly disabling neurodegenerative disease, clinically characterized by a progressive decline in cognitive functions, and is the most common form of dementia in the elderly. For decades, the search for disease-modifying therapies has focused on the two main Alzheimer's disease histopathological hallmarks, seeking to prevent, mitigate, or clear the formation of extracellular aggregates of β-amyloid peptide and intracellular neurofibrillary tangles of tau protein, although without clinical success. Mesenchymal stem cell-based therapy has emerged as a promising alternative for the treatment of Alzheimer's disease, especially because it also targets other crucial players in the pathogenesis of the disease, such as neuroinflammation, synaptic dysfunction/loss, oxidative stress, and impaired neurogenesis. Herein, we review current knowledge of the therapeutic potential of mesenchymal stem cells and their extracellular vesicles for Alzheimer's disease, discussing the most recent findings in both preclinical and clinical trials as well as how advanced technologies have helped to overcome some limitations and contributed to stimulate the development of more effective treatments.

关键词： Alzheimer's disease amyloid-β cell therapy cell transplantation exosomes extracellular vesicles mesenchymal stem cell micro vesicles neuroinflammation tau protein

Needle knife fstulotomy in flat and diverticular papillae:Is it time for redemption?

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Hepatobiliary & Pancreatic Diseases International 2022年第2期21卷 175-181页

作者： João Fernandes Jorge Canena Marta Moreira gonçalo Alexandrino Luísa Figueiredo Tarcísio Araújo Luís Lourenço David Horta Luís Lopes Department of Gastroenterology Santa Luzia Hospital-Unidade Local de Saúde Alto MinhoViana do CasteloPortugal Department of Gastroenterology Professor Doutor Fernando Fonseca HospitalAmadoraPortugal Department of Gastroenterology Nova Medical School/Faculty of Medical SciencesLisbonPortugal University Center of Gastrenterology-Hospital Cuf Tejo LisbonPortugal Life and Health Sciences Research Institute(ICVS) School of MedicineUniversity of MinhoBragaPortugal ICVS/3B’s-PT Government Associate Laboratory Braga/GuimarãesPortugal

Background: European Society of Gastrointestinal Endoscopy( ESGE) recommends needle-knife fstulotomy(NKF) as the preferred precut technique in cases when standard cannulation techniques fail. Despite scarce scientifc evidence, flat and diverticular papillae are thought not to be ideal for NKF, as they are associated with poor outcomes. The present study aimed to determine the outcomes of the use of NKF in relation to flat and intradiverticular papillae. Methods: This prospective multicenter study enrolled consecutive patients, evidencing na?ve flat(group A, n = 49) or diverticular papilla(group B, n = 28), who underwent NKF after failure of standard cannulation techniques. Diverticular morphology was subdivided into intradiverticular(group B1, n = 14) and diverticular border papillae(group B2, n = 14), using a previously validated endoscopic classifcation of the major papilla. The success of biliary cannulation at initial endoscopic retrograde cholangiopancreatography(ERCP), overall biliary cannulation, overall cannulation time, and the rate of adverse events were assessed in the study. Results: The initial cannulation rates were 93.9%, 64.3% and 71.4% for group A, B1, and B2, respectively( P = 0.005);overall cannulation rates after a second ERCP were 98.0%, 92.9% and 85.7%, respectively( P = 0.134). Adverse events occurred in 11.7% of patients, with post-ERCP pancreatitis(PEP) being the most common adverse event(10.4%). Although there was a trend towards a higher incidence of PEP in flat papillae, univariate and multivariate analyses did not show any signifcant relationship between pancreatitis and trainee involvement, papillary morphology, nor overall cannulation time. Conclusions: Although flat papillae are associated with high success rates of biliary cannulation using NKF, the rate of PEP is not negligible. NKF is feasible in diverticular papillae, but it is associated with a modest success rate in the initial ERCP.

关键词： Endoscopic retrograde cholangiopancreatography Needle-knife fstulotomy Small papilla Diverticular papilla Complications

Gangliosides in nervous system development,regeneration,and pathologies

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Neural Regeneration Research 2023年第1期18卷 81-86页

作者： Juliana F.Vasques Renata guedes de Jesus gonçalves Almir Jordão da Silva-Junior Robertta Silva Martins Fernanda Gubert Rosalia Mendez-Otero Instituto de Ciências Biomédicas Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de JaneiroRio de JaneiroBrazil Programa Redes de Pesquisa em Saúde no Estado do Rio de Janeiro Rio de JaneiroBrazil Programa Rede NanoSaúde do Estado do Rio de Janeiro Rio de JaneiroBrazil Instituto Nacional de Ciência e Tecnologia em Medicina Regenerativa Rio de JaneiroBrazil

Gangliosides,sialic acid-containing sphingolipids,are major constituents of neuronal *** to the number of sialic acids and the structure of the oligosaccharide chain,gangliosides can be classified as simple or complex and grouped in different *** of gangliosides have been identified in vertebrate cells,with different expression patterns during development and related to several physiological processes,especially in the nervous *** GD3 and its O-acetylated form,9acGD3,are highly expressed in early developmental stages,GM1,GD1a,GD1b,and GT1b are the most abundant ganglioside species in the mature nervous *** in enzymes involved in ganglioside metabolism can lead to the accumulation of specific species,a condition termed gangliosidosis and usually marked by severe neurological *** in ganglioside levels have also been described in several neurodegenerative diseases,such as Alzheimer’s and Parkinson’*** this review,we summarized recent information about the roles of GD3,9acGD3,GM1,GD1a,GD1b,GT1b,and other ganglioside species in nervous system development and regeneration,as well as clinical trials evaluating possible therapeutic applications of these molecules.

关键词： 9acGD3 gangliosides GD1a GD1b GD3 glycolipids GM1 GM2 GM3 GM4 GT1b

Prediction of future capacity and internal resistance of Li-ion cells from one cycle of input data

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能源与人工智能(英文) 2021年第3期5卷 209-216页

作者： Calum Strange gonçalo dos Reis School of Mathematics University of EdinburghThe King’s BuildingsEdinburghEH93FDUK Centro de Matemática e Aplicações(CMA) FCTUNLQuinta da Torre2829-516 CaparicaPortugal

There is a large demand for models able to predict the future capacity retention and internal resistance(IR)of Lithium-ion battery cells with as little testing as *** provide a data-centric model accurately predicting a cell’s entire capacity and IR trajectory from one single cycle of input *** represents a significant reduction in the amount of input data needed over previous *** approach characterises the capacity and IR curve through a small number of key points,which,once predicted and interpolated,describe the full *** this approach the remaining useful life is predicted with an 8.6%mean absolute percentage error when the input-cycle is within the first 100 cycles.

关键词： Capacity degradation Internal resistance degradation Prediction of full degradation curve Knee and elbow-points Lithium-ion cells Machine learning Remaining useful life

Lithium-ion battery data and where to find it

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能源与人工智能(英文) 2021年第3期5卷 255-269页

作者： gonçalo dos Reis Calum Strange Mohit Yadav Shawn Li School of Mathematics University of EdinburghThe King’s BuildingsEdinburgh EH93FDUK Centro de Matemática e Aplicações(CMA) FCTUNLQuinta da Torre2829-516 CaparicaPortugal Indian Institute of Technology KanpurIndia

Lithium-ion batteries are fuelling the advancing renewable-energy based *** the core of transformational developments in battery design,modelling and management is *** this work,the datasets associated with lithium batteries in the public domain are *** review the data by mode of experimental testing,giving particular attention to test variables and data *** highlighted tools and platforms,over 30 datasets are reviewed.

关键词： Lithium battery Public data Battery data Battery tests Machine learning

Automatic method for the estimation of li-ion degradation test sample sizesrequired to understand cell-to-cell variability

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能源与人工智能(英文) 2022年第3期9卷 139-147页

作者： Calum Strange Michael Allerhand Philipp Dechent gonçalo dos Reis School of Mathematics University of EdinburghThe Kings buildingsEdinburghEH93JFScotlandUnited Kingdom Institute for Power Electronics and Electrical Drives(ISEA) RWTH Aachen UniversityAachenGermany Centro de Matematica e Aplicacoes(CMA) Faculdade de Ciencias e TecnologiaCampus da CaparicaCaparica2829-516Portugal

The testing of battery cells is a long and expensive process, and hence understanding how large a test set needsto be is very useful. This work proposes an automated methodology to estimate the smallest sample size ofcells required to capture the cell-to-cell variability seen in a larger population. We define cell-to-cell variationbased on the slopes of a linear regression model applied to capacity fade curves. Our methodology determinesa sample size which estimates this variability within user specified requirements on precision and *** sample size is found using the distributional properties of the slopes under a normality assumption, andan implementation of the approach is available on *** the five datasets in the study, we find that a sample size of 8–10 cells (at a prespecified precision andconfidence) captures the cell-to-cell variability of the larger datasets. We show that prior testing knowledge canbe leveraged with machine learning models to operationally optimise the design of new cell-testing, leadingup to a 75% reduction in experimental costs.

关键词： Battery Testing Lithium-ion Degradation Statistics Manufacturing Machine learning