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Overview of the Analytical Lifecycle of Supercritical Fluid Chromatography Methods

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American Journal of Analytical Chemistry 2016年第1期7卷 75-91页

作者： Bertyl Andri Amandine Dispas Roland Djang’eing’a Marini Philippe hubert Department of Pharmacy CIRM Laboratory of Analytical Chemistry University of Liege (ULg) Liege Belgium

In recent times, the overall interest over Supercritical Fluid Chromatography (SFC) is truly growing within various domains but especially for pharmaceutical analysis. However, in the best of our knowledge modern SFC is not yet applied for drug quality control in the daily routine framework. Among the numerous reported SFC methods, none of them could be found to fully satisfy to all steps of the analytical method lifecycle. Thereby, the present contribution aims to provide an overview of the current and past achievements related to SFC techniques, with a targeted attention to this lifecycle and its successive steps. The included discussions were therefore structured accordingly and emphasizing the analytical method lifecycle in accord with the International Conference on Harmonisation (ICH). Recent and important scientific outputs in the field of analytical SFC, as well as instrumental evolution, qualification strategies, method development methodologies and discussions on the topic of method validation are reviewed.

关键词： Supercritical Fluid Chromatography Ultra High Performance Supercritical Fluid Chromatography Analytical Method Lifecycle Quality by Design

Femoral indicator injection for transpulmonary thermodilution using the eV1000/VolumeView~®: do the same criteria apply as for the PiCCO~®?

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第7期17卷 561-567页

作者： Wolfgang hubert Veit PHILLIP Josef HOLLTHALeR Caroline SCHULTHeISS Bemd SAUGeL Roland M. SCHMID Second Medical Department Klinikum rechts der lsar Technical University of Munich 81675 Munich Germany

Objective： Comparison of global end-diastolic volume index （GeDVI） obtained by femoral and jugular transpulmonary thermodilution （TPTD） indicator injections using the eV1000NolumnView device （edwards Lifesci- ences, Irvine, USA）. Methods： In an 87-year-old woman with hypovolemic shock and equipped with both jugular and femoral vein access and monitored with the eV1000NolumeView device, we recorded 10 datasets, each comprising duplicate TPTD via femoral access and duplicate TPTD （20 ml cold saline） via jugular access. Results： Mean femoral GeDVI （（674.6±52.3） ml/m2） was significantly higher than jugular GeDVI （（552.3±69.7） ml/m2）, with P=-0.003. Bland-Airman analysis demonstrated a bias of （＋122±61） ml/m2, limits of agreement of -16 and ＋260 ml/m2, and a percentage error of 22%. Use of the correction-formula recently suggested for the PiCCO device significantly reduced bias and percentage error. Similarly, mean values of parameters derived from GeDVI such as pulmonary vascular permeability index （PVPI; 1.244±0.101 vs. 1.522±0.139; P〈0.001） and global ejection fraction （GeF; （24.7±1.6）% vs. （28.1±1.8）%; P〈0.001） were significantly different in the case of femoral compared to jugular indicator injection. Fur- thermore, the mean cardiac index derived from femoral indicator injection （（4.50±0.36） L/（min.m2）） was significantly higher （P=0.02） than that derived from jugular indicator injection （（4.12±0.44） L/（min.m2））, resulting in a bias of （＋0.38±0.37） L/（min.m2） and a percentage error of 19.4%. Conclusions： Femoral access for indicator injection results in markedly altered values provided by the eV1000NolumeView , particularly for GeDVI, PVPI, and GeF.

关键词： Hemodynamic monitoring Transpulmonary thermodilution Central venous catheter Femoral vein Jugular vein Global end-diastolic volume eVlOOONolumeView PiCCO

Analysis of the genetic interactions between Cyclin A1, Atm and p53 during spermatogenesis

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Asian Journal of Andrology 2007年第6期9卷 739-750页

作者： Nicole Baiumer Marie-Luise Sandstede Sven Diederichs Gabriele Kohler Carol Readhead Ping Ji Feng Zhang etmar Bulk Jorg Gromoll Wolfgang e. Berdel hubert Serve Carsten Muller-Tidow Department of Medicine Hematology and Oncology University of Munster Domagstrasse 3 Munster 48149 Germany Department of Pathology University of Munster Domagstrasse 17 Munster 48149 Germany California Institute of Technology 1200 East California Boulevard Pasadena California 91125 USA Interdisciplinary Center for Clinical Research University of Munster Domagstrasse 3 Munster 48149 Germany Institute of Reproductive Medicine University of Munster Domagstrasse 12 Munster 48129 Germany

Aim： To analyze the functional interactions of Cyclin with p53 and Atm in spermatogenesis and DNA double- strand break repair. Methods： Two lines of double knockout mice were generated. Spermatogenesis and double strand break repair mechanisms were analyzed in Cyclin A1 （Ccnal）; p53- and Ccnal; Atm-double knockout mice. Results： The block in spermatogenesis observed in Cyclin A1-/- （Ccnal-/-） testes at the mid-diplotene stage is associated with polynucleated giant cells. We found that Ccnal-deficient testes and especially the giant cells accumulate unrepaired DNA double-strand breaks, as detected by immunohistochemistry for phosphorylated H2AX. In addition, the giant cells escape from apoptosis. The development of giant cells occurred in meiotic prophase I, because testes lacking ATM, which are known to develop spermatogenic arrest earlier than prophase I, do not develop giant cells in the absence of cyclin A1. Cyclin A1 interacted with p53 and phosphorylated p53 in complex with CDK2. Interestingly, p53-deficiency significantly increased the number of giant cells in Ccnal-deficient testes. Gene expression analyses of a panel of DNA repair genes in the mutant testes revealed that none of the genes examined were consistently misregulated in the absence of cyclin A1. Conclusion： Ccnal-deficiency in spermatogenesis is associated with defects in DNA double-strand break repair, which is enhanced by loss of p53.

关键词： spermatogenesis testis cell cycle meiosis DNA double-strand break giant cell knockout mice

endocrine disrupting chemicals in mixture and obesity,diabetes and related metabolic disorders

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World Journal of Biological Chemistry 2017年第2期8卷 108-119页

作者： Brigitte Le Magueresse-Battistoni emmanuel Labaronne hubert Vidal Danielle Naville CarMeN Laboratory IN SERM U1060Facultede Medecine Lyon-Sud Univ-Lyon Car Me N LaboratoryINSERM U1060INRA U1397Universite Claude Bernard Lyon1INSA LyonCharles Merieux Medical School Univ-Lyon CarMeN LaboratoryINSERM U1060INRA U1397Universite Claude Bernard Lyon1INSA LyonCharles Merieux Medical School

Obesity and associated metabolic disorders represent a major societal challenge in health and quality of life with large psychological consequences in addition to physical disabilities. They are also one of the leading causes of morbidity and mortality. Although, different etiologic factors including excessive food intake and reduced physical activity have been well identified, they cannot explain the kinetics of epidemic evolution of obesity and diabetes with prevalence rates reaching pandemic proportions. Interestingly, convincing data have shown that environmental pollutants, specifically those endowed with endocrine disrupting activities, could contribute to the etiology of these multifactorial metabolic disorders. Within this review, we will recapitulate characteristics of endocrine disruption. We will demonstrate that metabolic disorders could originate from endocrine disruption with a particular focus on convincing data from the literature. eventually, we will present how handling an original mouse model of chronic exposition to a mixture of pollutants allowed demonstrating that a mixture of pollutants each at doses beyond their active dose could induce substantial deleterious effects on several metabolic end-points. This proof-of-concept study, as well as other studies on mixtures of pollutants, stresses the needs for revisiting the current threshold model used in risk assessment which does not take into account potential effects of mixtures containing pollutants at environmental doses, e.g., the real life exposure. Certainly, more studies are necessary to better determine the nature of the chemicals to which humans are exposed and at which level, and their health impact. As well, research studies on substitute products are essential to identify harmless molecules.

关键词： endocrine disrupting chemicals Persistent organic pollutants Phthalates Bisphenol A Metabolic disorders Insulin resistance

Phytochemical Screening and Acute Toxicity evaluation of Leaves extract of Two Fabaceae’s Species: Sesbania pachycarpaDC. and Indigofera berhautianaGillett

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Journal of Biosciences and Medicines 2020年第7期8卷 28-34页

作者： Monique Brigitte Ouattara Jean hubert Bationo Martin Kiendrebeogo Odile Germaine Nacoulma Laboratoire de Biochimie et Chimie Appliquées (LABIOCA) Université Joseph KI-ZeRBO Unité de Formation et de Recherche en Sciences de la Vie et de la Terre Département de Biochimie-Microbiologie Ouagadougou Burkina Faso

Objective: The aim of this study is to screen chemical composition and to evaluate the acute general toxicity of leaves aqueous extracts from Sesbania pachycarpa DC. (Fabaceae), used in traditional medicine in Burkina... 详细信息

Objective: The aim of this study is to screen chemical composition and to evaluate the acute general toxicity of leaves aqueous extracts from em>Sesbania pachycarpaem> DC. (Fabaceae), used in traditional medicine in Burkina Faso to treat malaria, helminthiasis, and aqueous extracts of the leaves from Indigofera berhautiana Gillet (Fabaceae) used to treat hepatitis and typhoid fever. Methods: Aqueous decoction of leaves from em>Sesbania pachycarpaem> DC. or em>Indigofera berhautianaem> Gillett which are the form of use recommended by traditional health practitioners, were used for tests. Aqueous extracts used to evaluate the acute toxicity, were studied on mice of strain NMRI. For the toxicity study, the doses of the aqueous extracts were respectively 5 mg/kg, 50 mg/kg, 300 mg/kg and 2000 mg/kg of body weight. Phytochemical screening was done to identify the phytochemicals contained in extracts. Results: Aqueous extracts of two species: em>Sesbania pachycarpaem> DC., em>Indigofera berhautianaem> Gillet were not toxic at the maximum dose of 2000 mg/kg of body weight. Phytochemical screening, showed coumarins, flavonoids, tannins, polyphenols compounds, steroids and/or triterpenes and saponosides in the aqueous extracts of the two species. Alkaloids were identified in em>Sesbania pachycarpaem> aqueous extract.

关键词： Sesbania pachycarpa DC. Indigofera bergautiana Gillet Aqueous extracts Acute General Toxicity NMRI Strain Phytochemicals Compounds

effect of Storage Temperature on Vitamin C, Total Phenolics,UPLC Phenolic Acid Profile and Antioxidant Capacity of eleven Potato(Solanum tuberosum) Varieties

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Horticultural Plant Journal 2017年第2期3卷 73-89页

作者： Joseph hubert Yamdeu Galani Pooja M.Mankad Avadh K.Shah Nilesh J.Patel Rajeshkumar R.Acharya Jayant G.Talati Department of Agriculture and Veterinary Medicine Université des Montagnes Department of Biochemistry B.A. College of Agriculture Anand Agricultural University Main Vegetable Research Station Anand Agricultural University

Storage of potato tubers at low temperature affects their metabolism and may alter their phytochemical properties. There is a need to elucidate the changes in antioxidant compounds, activity and enzymes during storage of tubers. eleven Indian potato varieties were evaluated for antioxidant parameters, after 0, 30, 60 and 90 days of storage at room temperature, 15 °C and 4 °C. Total phenolics(0.0786–0.1546 mg gallic acid equivalents·g^(-1)FW) and vitamin C content(0.0828–0.2416 mg·g^(-1)FW) varied among the varieties and were different with storage temperature; their levels fluctuated during storage but remained above the initial level until the last day of observation. Phenolic acid profiling by UPLC identified 12 compounds among which the most abundant was chlorogenic acid followed by gallic acid, sinapic acid and ellagic acid. except para-coumaric acid which decreased at 4 °C, all the phenolic acids increased with storage. Caffeic acid, chlorogenic acid, protocatechuic acid and gallic acid mostly correlated with total phenolic content(r = 0.456, 0.482, 0.588 and 0.620, respectively). Antioxidant activity against both DPPH and ABTS radicals increased during the initial days of storage and then dropped to a level comparable or lower than the original value,irrespective of the storage temperature. Correlation study revealed that chlorogenic acid, gallic acid and ferulic acid mostly contributed to antioxidant activity. Activity of both antioxidant enzymes, superoxide dismutase and ascorbate peroxidase, increased initially but then decreased to values lower than the initial level and were not influenced by storage temperature. Correlation with antioxidant activity indicated that the enhancement of reactive oxygen scavenging species in cold stored tubers could result mainly from ascorbate peroxidase activity. Our results demonstrate that storage temperature adversely influences the metabolism and the content of antioxidant compounds in potato tubers, with subsequent inc

关键词： potato cold storage UPLC phenolics vitamin C antioxidant

efficacy of Selected Plant extracts against Pyricularia grisea, Causal Agent of Rice Blast Disease

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American Journal of Plant Sciences 2015年第5期6卷 602-611页

作者： Judith hubert Robert B. Mabagala Delphina P. Mamiro Department of Crop Science and Production Sokoine University of Agriculture Morogoro Tanzania Africa Rice Center Dar es Salaam Tanzania

Rice blast disease, caused by a seed-borne fungus Pyricularia grisea, is an important and serious disease of rice (Oryza sativa L.) worldwide. The disease has been reported to cause yield losses of up to 40% in Tanzania. Studies were conducted to determine the effect of aqueous extracts of Aloe vera, Allium sativum, Annona muricata, Azadirachta indica, Bidens pilosa, Camellia sinensis, Chrysanthemum coccineum, processed Coffee arabica, Datura stramonium, Nicotiana tabacum and Zingiber officinalis for control of rice blast disease (Pyricularia grisea) in-vitro and in-vivo. The results indicate that processed C. arabica at 10% and 25% (v/v) had the highest (81.12%) and (89.40%) inhibitory effect, respectively, against P. grisea. Aqueous extract from N. tabacum at 10% concentration ranked third (80.35%) in inhibiting P. grisea. These were followed by extracts from 25% A. vera (79.45%) and 25% C. coccineum flower (78.83%). The results also indicate that, extracts from A. indica, A. vera, A. sativum, C. arabica, D. stramonium, C. sinensis, Z. officinalis and N. tabacum did not have any phytotoxic effect on seed germination, shoot height, root length, dry weight, seedling growth and seedling vigour index. These plant extracts can thus be used for rice seed treatment to manage rice blast disease.

关键词： Plant extracts Pyricularia grisea Rice

Role of environmental pollution in irritable bowel syndrome

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World Journal of Gastroenterology 2015年第40期21卷 11371-11378页

作者： Mateusz Marynowski Aleksandra Likońska hubert Zatorski Jakub Fichna Department of Biochemistry Faculty of MedicineMedical University of Lodz Mossakowski Medical Research Centre Polish Academy of Sciences

Irritable bowel syndrome(IBS),with the prevalence of 10%-20 % of the population has become an emerging problem worldwide. IBS is a functional gastrointestinal(GI) disorder characterized by abdominal pain or discomfort and altered bowel habits. The etiology of IBS contains genetic,psychological,and immunological factors,and has not been fully elucidated; of note,recent studies also point at environmental pollution and its role in the development of functional GI diseases. In this review we focus on several environmental factors,such as bacterial contamination,air pollution,radiation and even stress as potential triggers of IBS. We discuss associated disturbances in homeostasis,such as changes in intestinal microbiome and related pathophysiological mechanisms. Based on the effect of environmental factors on the GI tract,we also propose novel targets in IBS treatment.

关键词： Irritable bowel syndrome environmental pollution A

evaluation of Acute Toxicity, Antioxidant and Antibacterial Potential of Leaves extracts from Two Anacardiaceae’s Species: Lannea microcarpa engl. &K. Krause and Mangifera indica L.

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Journal of Biosciences and Medicines 2022年第10期10卷 125-134页

作者： Monique Brigitte Ouattara Jean hubert Bationo Martin Kiendrebeogo Odile Germaine Nacoulma Laboratoire de Biochimie et Chimie Appliquées (LABIOCA) Université Joseph KI-ZERBO Unité de formation et de recherche en sciences de la vie et de la terre Département de Biochimie-Microbiologie Ouagadougou Burkina Faso

Background: Leaves of Mangifera indica L or Lannea microcarpa engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this study is to evaluate the acute general toxicity, antioxidant potential and antibacterial activitiy of leave’s aqueous extracts from Lannea microcarpa engl. and K. Krause and Mangifera indica L. The use of these plants in traditional medicine motivated our choice to lead scientific studies. Methods: The aqueous decoction of the leaves is the form of use recommended by traditional healers. We used the same type of extracts for studies. Acute toxicity was studied in NMRI strain mice, with the dose of 2000 mg/kg body weight, for each plant species. The antioxidant activity is evaluated by the method of reduction of radical DPPH. The phytochemical compounds were detected with specific reagent: Alcaloids with Dragendorff’s reagent, Flavonoids with ammonia (NH4OH), Polyphenols and tannins are revelated by ferric chloride (FeCl3). Saponosides were revelated by their foaming power property. Bacterial inhibiting activity is tested by measuring the diameters’ inhibition of extracts on reference strains. Results: The aqueous extracts were not toxic at the maximum dose of 2000 mg/kg body weight, for each plant species. The extracts showed an antioxidant activity with an IC50, 20 μg/ml for Lannea microcarpa and 18 μg/ml for Mangifera indica. The extracts showed no bacterial activity on three strains of bacteria tested: escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The phytochemicals we have identified are for L. microcarpa: tannins and phenolics compounds, triterpenes, saponosides. We identified in M. indica’s extracts: tannins and phenolics compounds, triterpenes, saponosides and flavonoids.

关键词： Lannea microcarpa Mangifera indica Toxicity Antioxidant Activity DPPH Antibacterial Activity Phytochemical Compounds