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EDDS对土壤铜镉有效性及蓖麻吸收转运的影响

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环境科学 2024年第3期45卷 1803-1811页

作者：刘文英吴刚胡红青华中农业大学资源与环境学院武汉430070 重庆市农业科学院重庆401329

为探究螯合剂对植物吸收重金属的影响,以蓖麻(Ricinus communis L.)为供试植物,通过土培和盆栽试验,研究不同含量乙二胺二琥珀酸(EDDS)对土壤中铜镉形态和植物吸收、转运的影响.结果表明,EDDS显著增加了土壤有效态铜和镉含量,培养15 d时... 详细信息

为探究螯合剂对植物吸收重金属的影响,以蓖麻(Ricinus communis L.)为供试植物,通过土培和盆栽试验,研究不同含量乙二胺二琥珀酸(EDDS)对土壤中铜镉形态和植物吸收、转运的影响.结果表明,EDDS显著增加了土壤有效态铜和镉含量,培养15 d时,增幅分别为43.01%~103.55%和51.78%~69.43%,同时促进了可还原态铜向弱酸提取态转化,增加了土壤铜的移动性.EDDS促进了蓖麻对铜的吸收、转运与富集.EDDS 2.5和EDDS 5.0处理时,地上部铜含量是对照的4.88倍和16.65倍(P<0.05),根部是对照的2.89倍和3.60倍(P<0.05),铜转运系数显著提高了72.73%和381.82%.EDDS 5.0处理时,蓖麻地上部和根部的铜提取量分别是对照处理的14.08倍和2.16倍,总铜提取量是对照处理的4.70倍(P<0.05).此外,EDDS显著增加了蓖麻镉含量,EDDS 2.5处理时,地上部和根部分别增加了15.15%和57.42%,蓖麻总镉提取量显著提高了13.44%.综上可知,EDDS能增加土壤铜镉的有效性,促进蓖麻对铜镉的吸收,提高蓖麻的修复效率,其中5.0 mmol·kg^(-1)EDDS更有利于蓖麻对铜的提取,而2.5 mmol·kg^(-1)EDDS处理对镉的提取有较高的增加效果.

关键词：铜(Cu) 镉(Cd) 乙二胺二琥珀酸(EDDS) 蓖麻植物修复重金属形态

DPHL:A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery

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Genomics, Proteomics & Bioinformatics 2020年第2期18卷 104-119页

作者： Tiansheng Zhu Yi Zhu Yue Xuan Huanhuan Gao Xue Cai Sander R.Piersma Thang V.Pham Tim Schelfhorst Richard R.G.D.Haas Irene V.Bijnsdorp Rui Sun Liang Yue Guan Ruan Qiushi Zhang Mo Hu Yue Zhou Winan J.Van Houdt Tessa Y.S.Le Large Jacqueline Cloos Anna Wojtuszkiewicz Danijela Koppers-Lalic Franziska Bottger Chantal Scheepbouwer Ruud H.Brakenhoff Geert J.L.H.van Leenders Jan N.M.Ijzermans John W.M.Martens Renske D.M.Steenbergen Nicole C.Grieken Sathiyamoorthy Selvarajan Sangeeta Mantoo Sze S.Lee Serene J.Y.Yeow Syed M.F.Alkaff Nan Xiang Yaoting Sun Xiao Yi Shaozheng Dai Wei Liu Tian Lu Zhicheng Wu Xiao Liang Man Wang yingkuan Shao Xi Zheng Kailun Xu Qin Yang Yifan Meng Cong Lu Jiang Zhu Jin'e Zheng Bo Wang Sai Lou Yibei Dai Chao Xu Chenhuan Yu huazhong ying Tony K.Lim Jianmin Wu Xiaofei Gao Zhongzhi Luan Xiaodong Teng Peng Wu Shi'ang Huang Zhihua Tao Narayanan G.Iyer Shuigeng Zhou Wenguang Shao Henry Lam Ding Ma Jiafu Ji Oi L.Kon Shu Zheng Ruedi Aebersold Connie R.Jimenez Tiannan Guo Zhejiang Provincial Laboratory of Life Sciences and Biomedicine Westlake UniversityHangzhou 310024China Key Laboratory of Structural Biology of Zhejiang Province School of Life SciencesWestlake UniversityHangzhou 310024China Institute of Basic Medical Sciences Westlake Institute for Advanced StudyHangzhou 310024China School of Computer Science Shanghai Key Laboratory of Intelligent Information ProcessingFudan UniversityShanghai 200433China Thermo Fisher Scientific(BREMEN)GmbH Bremen 28195Germany OncoProteomics Laboratory Department of Medical OncologyVU University Medical CenterVU UniversityAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamUrologyCancer Center AmsterdamAmsterdam 1011The Netherlands Thermo Fisher Scientific Shanghai 201206China The Netherlands Cancer Institute Surgical OncologyAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamSurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamPediatric Oncology/HematologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamHematologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamMedical OncologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamNeurosurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamPathologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamOtolaryngology/Head and Neck SurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Erasmus MC University Medical Center PathologyRotterdam 1016LVThe Netherlands Erasmus MC University Medical Center SurgeryRotterdam 1016LVThe Netherlands Erasmus MC University Medical Center Medical OncologyRotterdam 1016LVThe Netherlands Department of Anatomical Pathology Singapore General HospitalSingapore 169608Singapore Division of Medical Scie

To address the increasing need for detecting and validating protein biomarkers in clinical specimens,mass spectrometry(MS)-based targeted proteomic techniques,including the selected reaction monitoring(SRM),parallel reaction monitoring(PRM),and massively parallel dataindependent acquisition(DIA),have been *** optimal performance,they require the fragment ion spectra of targeted peptides as prior *** this report,we describe a MS pipeline and spectral resource to support targeted proteomics studies for human tissue *** build the spectral resource,we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on *** then applied the workflow to generate DPHL,a comprehensive DIA pan-human library,from 1096 data-dependent acquisition(DDA)MS raw files for 16 types of cancer *** extensive spectral resource was then applied to a proteomic study of 17 prostate cancer(PCa)***,PRM validation was applied to a larger study of 57 PCa patients and the differential expression of three proteins in prostate tumor was *** a second application,the DPHL spectral resource was applied to a study consisting of plasma samples from 19 diffuse large B cell lymphoma(DLBCL)patients and 18 healthy control *** expressed proteins between DLBCL patients and healthy control subjects were detected by DIA-MS and confirmed by *** data demonstrate that the DPHL supports DIA and PRM MS pipelines for robust protein biomarker *** is freely accessible at https://***/page/***?id=IPX0001400000.

关键词： Data-independent acquisition Parallel reaction monitoring Spectral library Prostate cancer Diffuse large B cell lymphoma

Melatonin attenuatesβ-amyloid-induced inhibition of neurofilament expression

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Acta Pharmacologica Sinica 2004年第4期25卷 49-53页

作者： ying-chun ZHANG, Ze-fen WANG, Qun WANG, Yi-pengWANG, Jian-zhi WANG2 Department of Pathophysiology, Tongji Medical College, huazhong University ofScience and Technology, Wuhan 430030, China Department of Pathophysiology Tongji Medical College Huazhong University of Science and Technology

AIM: To explore the effect of β-amyloid (Aβ) on metabolism of cytoskeletal protein neurofilament, and search for effective cure to the lesion. METHODS: Wild type murine neuroblastoma N2a (N2awt) and N2a stably transfected with wild type amyloid precursor protein (N2aAPP) were cultured. Sandwich ELISA, immunocytochemistry, and Western blot were used respectively to measure the level of Aβ, the expression and phosphorylation of neurofilament proteins. RESULTS: The immunoreactivity of neurofilament protein was almost abolished in N2aAPP, which beard a significantly higher level of Aβ. Melatonin effectively decreased the level of Aβ, and restored partially the level of phosphorylated and non-phosphorylated neurofilament in N2aAPP. CONCLUSION: Overproduction of Aβ inhibits neurofilament expression, and melatonin attenuates the Aβ-induced lesion in cytoskeletal protein.

关键词： Alzheimer disease amyloid beta-protein neurofilament proteins melatonin

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二代高温超导带材失超特性研究

电工技术学报 2024年第1期39卷 110-120,153页

作者：杨志星任丽徐颖段璞李鑫强电磁工程与新技术国家重点实验室(华中科技大学电气与电子工程学院) 武汉430074

高温超导带材失超会降低其通流能力,从而影响高温超导装置的运行稳定性。目前,高温超导带材失超仿真模型多为二维计算模型,无法准确模拟其失超过程中的电磁热行为。该文采用有限元方法对二代高温超导带材失超特性进行多物理场耦合分析... 详细信息

高温超导带材失超会降低其通流能力,从而影响高温超导装置的运行稳定性。目前,高温超导带材失超仿真模型多为二维计算模型,无法准确模拟其失超过程中的电磁热行为。该文采用有限元方法对二代高温超导带材失超特性进行多物理场耦合分析。在三维直角坐标系下,以磁场强度矢量为求解变量,建立了二代高温超导带材失超演化电磁热仿真模型,研究了二代高温超导带材长度方向与宽度方向在不同热扰动工况下的电磁热特性及其演化过程,分析了高温超导带材过电流失超过程中的各层分流状况与超导层电阻率变化行为,区分了不同类型的过电流失超行为,并提出了高温超导带材过电流冲击失超的电阻率判据。

关键词：二代高温超导带材失超特性有限元电磁热耦合

Millimeter waves in medical applications:status and prospects

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Intelligent Medicine 2024年第1期4卷 16-21页

作者： Honglin Wang Lin Lu Pengran Liu Jiayao Zhang Songxiang Liu Yi Xie Tongtong Huo Hong Zhou Mingdi Xue ying Fang Jiaming Yang Zhewei Ye Department of Orthopedics Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430022China Departments of Orthopedics Renmin Hospital of Wuhan UniversityWuhanHubei 430060China

Millimeter waves are electromagnetic waves with wavelengths of 1–10 mm,which have characteristics of high frequency and short *** have gradually and widely been used in engineering and medical *** have identified studies related to millimeter waves in the biomedical field and summarized the biological effects of millimeter waves and their current status in medical ***,the shortcomings of existing studies and future developments were analyzed and discussed,with the aim of providing a reference for further research and development of millimeter waves in the medical field.

关键词： Millimeter wave Biological effects Medical applications Vital signs monitoring

A Decision Model for Selecting Participants in Supply Chain

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Journal of Shanghai University(English Edition) 2001年第4期5卷 341-344页

作者： ZHOU Shui ying, CHEN Rong qiu School of Management, huazhong University of Science & Technology, Wuhan, Hubei 430079, China School of Management Huazhong University of Science & Technology Wuhan China

In order to satisfy the rapid changing requirements of customers, enterprises must cooperate with each other to form supply chain. The first and the most important stage in the forming of supply chain is the selection of participants. The article proposes a two staged decision model to select partners. The first stage is the inter company comparison in each business process to select high efficiency candidate based on inside variables. The next stage is to analyse the combination of different candidates in order to select the most perfect partners according to a goal programming model.

关键词： supply chain goal programming participants

Effect of amyloid peptides on serum withdrawal-induced cell differentiation and cell viability

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Cell Research 2004年第6期14卷 467-472页

作者： Yi Peng WANG, Ze Fen WANG, ying Chun ZHANG, Qing TIAN, Jian Zhi WANG Department of Pathophysiology, Tongji Medical College, huazhong University of Science and Technology, Wuhan, 430030, China Department of Pathophyiiology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Abnormal deposition of amyloid-p(Ap) peptides and formation of neuritic plaques are recognized as pathological processes in Alzheimer’s disease (AD) brain. By using amyloid precursor protein (APP) transfected cells, this study aims to investigate the effect of overproduction of Aβ on cell differentiation and cell viability. It was shown that after serum withdrawal, untransfected cell (N2a/Wt) and vector transfected cells (N2a/vector) extended long and branched cell processes, whereas no neurites was induced in wild type APP (N2a/APP695) and Swedish mutant APP (N2a/ APPswe) transfected N2a cells. After differentiation by serum withdrawal, the localization of APP/AP and neurofilament was extended to neurites, whereas those of APP-transfected cells were stillrestricted within the cell body. Levels of both APP and Aβ were significantly higher in N2a/APP695 and N2a/APPswe than in N2a/Wt, as determined by Western blot and Sandwich ELISA, respectively. To further investigate the effect of A0 on the inhibition

关键词： Alzheimer’s disease amyloid β cell differentiation.

Network Security Transmission Based on Bimatrix Game Theory

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Wuhan University Journal of Natural Sciences 2006年第3期11卷 617-620页

作者： ZHENG ying HU Hanping GUO Wenxuan Institute of Image Recognition and Artificial Intelligence Huazhong University of Science and Technology Wuhan 430074 Hubei China

Based on the bimatrix game theory, the network data transmission has been depicted in a game theory way： the actions of the attacker and defender （legitimate users） are depicted within a two-person, non-cooperative and bimatrix game model, this paper proves the existence of the Nash equilibrium theoretically, which is further illustrated by the experimental resuhs.

关键词： security transmission game theory bimatrix game Nash equilibrium

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基于对比学习和预训练模型的临床诊断标准化

华中科技大学学报（自然科学版） 2024年第5期52卷 23-28页

作者：刘莹崔丙剑曹琉程龙龙天津大学医学工程与转化医学研究院天津300072 中电云脑(天津)科技有限公司天津300300

针对临床诊断标准化任务中存在的标准诊断词库规模大、文本相关性不显著且标准词个数不确定的问题,提出一种基于对比学习和预训练模型的临床诊断标准化方法.先用无监督和有监督相结合的方法对基于简单对比学习的句子嵌入(SimCSE)模型进... 详细信息

针对临床诊断标准化任务中存在的标准诊断词库规模大、文本相关性不显著且标准词个数不确定的问题,提出一种基于对比学习和预训练模型的临床诊断标准化方法.先用无监督和有监督相结合的方法对基于简单对比学习的句子嵌入(SimCSE)模型进行训练,并利用得到的模型从标准库中召回候选标准词,再利用基于转换器的双向编码表征(BERT)进行候选词重排序和标准词个数分类,最终得到标准化结果.实验结果表明:基于无监督和有监督相结合的SimCSE方法的召回率为86.76%,显著优于其他方法;在重排序和标准词个数分类任务中,相比于其他模型,BERT在多个指标上有明显提升;该方法在测试集上进行标准词预测的F1值达到72.54%,在临床诊断标准化中具有较好的表现.

关键词：临床诊断标准化对比学习预训练模型基于简单对比学习的句子嵌入(SimCSE) 基于转换器的双向编码表征(BERT)