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中国现代应用药学 2016年第1期33卷 12-15页

作者：王丹仙马月方杰胡毅翔俞文英余陈欢应华忠浙江中医药大学药学院杭州310053 浙江省医学科学院浙江省实验动物与安全性研究重点实验室杭州310013

目的鉴定玉麦中可吸收入血的micro RNAs(mi RNAs)类活性物质,并分析其靶基因功能。方法采用二代高通量测序法,以正常小鼠血浆mi RNAs为空白对照,测定喂食玉麦匀浆液小鼠血浆中mi RNAs的总量,并与玉麦mi RNAs比对,鉴定吸收入体内的玉麦mi... 详细信息

目的鉴定玉麦中可吸收入血的micro RNAs(mi RNAs)类活性物质,并分析其靶基因功能。方法采用二代高通量测序法,以正常小鼠血浆mi RNAs为空白对照,测定喂食玉麦匀浆液小鼠血浆中mi RNAs的总量,并与玉麦mi RNAs比对,鉴定吸收入体内的玉麦mi RNAs。采用Target Scan与Mi Randa软件,预测玉麦mi RNAs可能作用于人类m RNA靶基因,并通过Gene Ontology(GO)与Kyoto Encyclopedia of Genes and Genomes(KEGG)分析靶基因的相关功能和信号途径。结果共鉴定出12个可吸收入体内的玉麦mi RNAs。GO与KEGG分析显示这些mi RNAs可能调控的靶基因及其参与的生物学功能包括胰岛素信号通路、癌症通路、MAPK通路等相关信号通路,其中mi R4995、mi R156a、mi R396e为玉麦可能的主要核酸活性物质。结论 mi RNAs可能为玉麦中的一种新的活性物质。

关键词：药效物质高通量测序靶基因

Molecular and therapeutic landscape of ferroptosis in skin diseases

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中华医学杂志(英文版) 2024年第15期137卷 1777-1789页

作者： Le Jiayuan Meng Yu Wang ying Li Daishi Zeng Furong Xiong Yixiao Chen Xiang Deng Guangtong Department of Dermatology Xiangya Hospital Central South University Changsha Hunan China National Engineering Research Center of Personalized Diagnostic and Therapeutic Technology Changsha Hunan China Furong Laboratory Changsha Hunan China Hunan Key Laboratory of Skin Cancer and Psoriasis Hunan Engineering Research Center of Skin Health and Disease Xiangya Hospital Central South University Changsha Hunan China National Clinical Research Center for Geriatric Disorders Xiangya Hospital Changsha Hunan China Department of Oncology Xiangya Hospital Central South University Changsha Hunan China Department of Dermatology Tongji Hospital Huazhong University of Science and Technology Wuhan Hubei China

Regulated cell death (RCD) is a critical physiological process essential in maintaining skin homeostasis. Among the various forms of RCD, ferroptosis stands out due to its distinct features of iron accumulation, lipid peroxidation, and involvement of various inhibitory antioxidant systems. In recent years, an expanding body of research has solidly linked ferroptosis to the emergence of skin disorders. Therefore, understanding the mechanisms underlying ferroptosis in skin diseases is crucial for advancing therapy and prevention strategies. This review commences with a succinct elucidation of the mechanisms that underpin ferroptosis, embarks on a thorough exploration of ferroptosis’s role across a spectrum of skin conditions, encompassing melanoma, psoriasis, systemic lupus erythematosus (SLE), vitiligo, and dermatological ailments precipitated by ultraviolet (UV) exposure, and scrutinizes the potential therapeutic benefits of pharmacological interventions aimed at modulating ferroptosis for the amelioration of skin diseases.

关键词： Ferroptosis Skin diseases Regulated cell death Homeostasis Melanoma Psoriasis Systemic lupus erythematosus Vitiligo

Therapeutic Effectiveness of Leukocyte-and Platelet-rich Fibrin for Diabetic Foot Ulcers:A Retrospective Study

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Current Medical Science 2024年第3期44卷 568-577页

作者： Fen WANG Xiao-ling ZHANG Jing ZHANG Song GONG Jing TAO Hui XIANG Xiao-qing FU Xu-na BIAN Xue-feng YU An-hui XU Cheng-la YI Shi-ying SHAO Department of Endocrinology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Branch of National Clinical Research Center for Metabolic Diseases Wuhan430030China Department of Radiology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China Department of Trauma Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Diabetic foot ulcer(DFU)is one of the most serious complications of ***-and platelet-rich fibrin(L-PRF)is a second-generation autologous platelet-rich *** study aims to investigate the clinical effects of L-PRF in patients with diabetes in real clinical *** Patients with DFU who received L-PRF treatment and standard of care(SOC)from 2018 to 2019 in Tongji Hospital were *** clinical information including patient characteristics,wound evaluation(area,severity,infection,blood supply),SOC of DFU,and images of ulcers was retrospectively extracted and analyzed.L-PRF treatment was performed every 7±2 days until the ulcer exhibited complete epithelialization or an overall percent volume reduction(PVR)greater than 80%.Therapeutic effectiveness,including overall PVR and the overall and weekly healing rates,was *** Totally,26 patients with DFU were enrolled,and they had an ulcer duration of 47.0(35.0,72.3)*** severity and infection of ulcers varied,as indicated by the Site,Ischemia,Neuropathy,Bacterial Infection,and Depth(SINBAD)scores of 2–6,Wagner grades of 1–4,and the Perfusion,Extent,Depth,Infection and Sensation(PEDIS)scores of 2–*** initial ulcer volume before L-PRF treatment was 4.94(1.50,13.83)cm3,and the final ulcer volume was 0.35(0.03,1.76)*** median number of L-PRF doses was 3(2,5).A total of 11 patients achieved complete epithelialization after the fifth week of treatment,and 19 patients achieved at least an 80%volume reduction after the seventh *** overall wound-healing rate was 1.47(0.63,3.29)cm3/week,and the healing rate was faster in the first 2 weeks than in the remaining *** treatment did not change the percentage of complete epithelialization or healing *** Adding L-PRF to SOC significantly improved wound healing in patients with DFU independent of the ankle brachial index,SINBAD score,or Wagner grade,indicating that this method is appropriate for D

关键词： diabetic foot ulcer autologous platelet-rich plasma leukocyte-and platelet-rich fibrin standard of care percentage volume reduction

Inhibition of Bcl-6 Expression Ameliorates Asthmatic Characteristics in Mice

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Current Medical Science 2024年第1期44卷 110-120页

作者： Chang-zhi ZHOU Xiong XIONG Wei-jun TAN Ya-fei WANG Zhen YANG Xue-ying LI Xiu-wen YANG Xiao-fan LIU Sun-feng YU Liang-chao WANG Shuang GENG Department of Respiratory and Critical Care Medicine The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Department of General Surgery Wuhan Wuchang HospitalWuhan University of Science and TechnologyWuhan 430063China

Objective The function of Bcl-6 in T follicular helper(Tfh)cell maturation is indispensable,and Tfh cells play a pivotal role in *** study investigated the impact of Bcl-6 on asthmatic *** The microscopic pathological alterations,airway resistance(AR),and lung compliance(LC)were determined in asthmatic mice and Bcl-6 interference *** surface molecular markers of Tfh cells and the Bcl-6 mRNA and protein expression were determined by flow cytometry,RT-qPCR,and Western blotting,*** relationships between the Tfh cell ratio and the IgE and IgG1 concentrations in peripheral blood mononuclear cells(PBMCs)and bronchoalveolar lavage fluid(BALF)were *** Asthmatic inflammatory changes were observed in the lung tissue and were attenuated by Bcl-6 siRNA and dexamethasone(DXM).Asthmatic mice exhibited an increased AR and a decreased LC,while Bcl-6 siRNA or DXM mitigated these *** percentages of Tfh cells and eosinophils were significantly increased in the asthmatic mice,and they significantly decreased after Bcl-6 inhibition or DXM ***-qPCR and Western blotting analyses revealed that the Bcl-6 expression level in PBMCs was significantly higher in asthmatic mice,and it decreased following Bcl-6 inhibition or DXM *** IgE expression in the serum and BALF and the B cell expression in PBMCs exhibited a similar *** asthmatic mice,the ratio of Tfh cells in the peripheral blood showed a strong positive correlation with the IgE levels in the serum and BALF,but not with the IgG1 *** The amelioration of airway inflammation and airway hyper-responsiveness is achieved through Bcl-6 suppression,which effectively hinders Tfh cell differentiation,ultimately resulting in a concurrent reduction in IgE production.

关键词： Bcl-6 Tfh asthma airway hyper-responsiveness mouse model

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蜜蜂幼虫、蛹抗衰老作用的实验研究

特产研究 1997年第2期19卷 13-15页

作者：胡福良陈民利陈盛禄应华忠王德军浙江农业大学蜂业研究所 310029 浙江中医学院实验动物中心 310009

用工蜂幼虫、蛹、蜂王幼虫灌胃老年Wristar大鼠10天后，分别测定大鼠脑、肝脏和血液中SOD活力和MDA含量。结果发现，SOD活力均显著提高，MDA含量均显著下降，这反映了工蜂幼虫、蛹与蜂王幼虫一样具有明显的抗衰老作用。

关键词：工蜂幼虫蛹蜂王幼虫抗衰老

三黄洗剂改善特应性皮炎小鼠模型皮肤屏障的机制探讨

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中国中西医结合皮肤性病学杂志 2021年第5期20卷 457-460页

作者：陈文颖刘冬梅王坤兰燕琴解凡沈珍罗宏宾应华忠金华市第五医院浙江金华321000 浙江省中医院浙江杭州310002 浙江省医学科学院实验动物中心浙江杭州310013

目的探讨三黄洗剂改善特应性皮炎(AD)小鼠模型皮肤屏障可能的作用机制。方法取60只小鼠随机分为对照组、模型组、复方地塞米松软膏组、三黄洗剂低剂量组、中剂量组、高剂量组,涂抹2,4-二硝基氯苯丙酮溶液造模。每天涂抹给药2周后,采用... 详细信息

目的探讨三黄洗剂改善特应性皮炎(AD)小鼠模型皮肤屏障可能的作用机制。方法取60只小鼠随机分为对照组、模型组、复方地塞米松软膏组、三黄洗剂低剂量组、中剂量组、高剂量组,涂抹2,4-二硝基氯苯丙酮溶液造模。每天涂抹给药2周后,采用免疫组化法检测中间丝聚合蛋白(FLG)、水通道蛋白3(AQP3)和角蛋白17(K17)的表达,称量皮肤湿重、干重,计算失重比。结果模型组、三黄洗剂低、中、高剂量组以及复方地塞米松乳膏组皮肤失重比比正常对照组增加且差异有统计学意义(P<0.01)。模型组FLG的表达明显低于正常对照组,三黄洗剂低、中、高剂量组和复方地塞米松乳膏组FLG表达量高于模型组。而模型组AQP3、K17的表达均明显高于正常对照组,三黄洗剂低、中、高剂量组和复方地塞米松乳膏组AQP3、K17表达量均低于模型组。结论三黄洗剂治疗AD小鼠后能减轻炎性水肿,提高FLG含量,降低AQP3、K17的表达,可证明其能促进皮肤屏障的修复。

关键词：特应性皮炎动物模型三黄洗剂皮肤屏障中间丝聚合蛋白水通道蛋白3 角蛋白17

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城市多中心结构提升了居民幸福感吗?

华中科技大学学报（社会科学版） 2024年第5期38卷 29-40页

作者：张可钟莹东华大学旭日工商管理学院华东政法大学商学院

城市是承载美好生活的空间载体,城市空间发展模式对居民福祉的影响不可忽视。本文基于2010—2018年中国家庭追踪调查(CFPS)的微观数据,采用多层混合效应模型考察了城市多中心结构对城市居民幸福感的影响。研究发现,城市多中心结构显著... 详细信息

城市是承载美好生活的空间载体,城市空间发展模式对居民福祉的影响不可忽视。本文基于2010—2018年中国家庭追踪调查(CFPS)的微观数据,采用多层混合效应模型考察了城市多中心结构对城市居民幸福感的影响。研究发现,城市多中心结构显著提升了居民幸福感,这一结论经系列稳健性检验和解决内生性问题后依然成立。异质性分析发现,多中心空间结构对大城市、高购房压力城市以及城市户籍、高收入阶层居民幸福感的促进作用更大。机制检验表明,城市多中心结构通过就业和收入效应、公共服务和消费效应、居住环境效应三种潜在机制影响居民幸福感。本文为优化城市空间结构和增进民生福祉的双协同提供了经验证据。

关键词：城市空间结构居民幸福感影响机制

卵细胞体外成熟(IVM)在小鼠生物净化中的应用

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实验动物科学 2019年第3期36卷 53-57页

作者：杜江涛武晓静戴方伟卢领群周莎桑应华忠郭红刚浙江省医学科学院浙江省实验动物中心杭州310013 杭州市中医院杭州310007

目的在小鼠生物净化中,从超排后未排卵的卵巢,取卵泡内未成熟卵采用卵母细胞体外成熟(in vitro maturation,IVM)使之在体外成熟并具备受精能力,以提高卵子利用率和作为常规促超排失败的一种补救措施。方法在小鼠生物净化中,取注射PMSG和... 详细信息

目的在小鼠生物净化中,从超排后未排卵的卵巢,取卵泡内未成熟卵采用卵母细胞体外成熟(in vitro maturation,IVM)使之在体外成熟并具备受精能力,以提高卵子利用率和作为常规促超排失败的一种补救措施。方法在小鼠生物净化中,取注射PMSG和HCG后未排卵卵巢,在实体显微镜下划破卵泡挑选卵丘卵母细胞复合物(COCs),置于成熟液滴中在体外发育成熟。同时以注射PMSG和HCG后正常超排卵、只注射PMSG后取未成熟卵体外成熟和注射PMSG和HCG后取疑似成熟卵和裸卵体外成熟作为对照。经体外受精、体外胚胎发育后,将2-细胞胚移植到受体输卵管,使其在受体内发育成为成熟的个体。结果注射PMSG和HCG后未排卵组(A组),卵细胞体外成熟率为87.0%±3.2%,二胞率为55.1%±12.3%,囊胚率为23.1%,移植41枚二细胞胚至2只受体鼠,出生5只幼崽,产仔率12.2%。只注射PMSG,48 h后取未成熟卵组(B组),体外成熟率为83.9%±3.9%,二胞率为51.8%±9.3%,囊胚率为38.5%±13.9%。注射PMSG和HCG正常超排组(C组),其二胞率为78.9%±0.6%,囊胚率为78.0%±3.8%。注射PMSG和HCG未排卵卵巢,取裸卵和疑似成熟卵(D组),体外成熟培养0 h,6 h和16~18 h,其成熟率、二胞率和囊胚率与其它三组相比均较低且有极显著性差异。A组和B组与正常对照C组相比,二胞率均有显著性差异,囊胚率均有极显著性差异。结论卵母细胞体外成熟(IVM)可以作为小鼠生物净化中促超排失败的一种补救措施,并且可以提高珍稀品系小鼠的卵子利用率。

关键词：小鼠卵母细胞体外成熟(IVM) 生物净化

Isolation of a feline-derived feline panleukopenia virus with an A300P substitution in the VP2 protein and confrmation of its pathogenicity in dogs

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Animal Diseases 2024年第3期4卷 173-185页

作者： Jiakang Li Jiajia Peng Yue Zeng ying Wang Luying Li Yiran Cao Longlong Cao QingXiu Chen Zijun Ye Dengyuan Zhou Shengbo Cao Qiuyan Li National Key Laboratory of Agricultural Microbiology Huazhong Agricultural UniversityWuhan 430070HubeiChina. Laboratory of Animal Virology College of Veterinary MedicineHuazhong Agricultural UniversityWuhanHubeiP.R.China. Wuhan Keqian Biology Co. LtdWuhanHubeiP.R.China. Department of Life Science Imperial College LondonLondonUK.

Feline panleukopenia virus(FPV)is a single-stranded DNA virus that can infect cats and cause feline panleukopenia,which is a highly contagious and fatal disease in *** sequence of FPV is highly variable,and mutations in the amino acids of its capsid protein play crucial roles in altering viral virulence,immunogenicity,host selection,and other *** this study,the epidemiology of FPV was studied using 746 gastrointestinal swab samples derived from cats that presented gastrointestinal symptoms specifcally,diarrhea or vomiting during the period spanning from 2018 to *** overall prevalence of FPV-positive patients among these samples was determined to be 45.4%.Capsid(virion)protein 2(VP2)gene of each FPV-positive sample was sequenced and amplifed,yielding 65 VP2 *** them,six VP2 gene sequences were detected in the majority of the samples test positive for FPV,and these positive samples originated from a diverse range of geographical *** isolates were named FPV-6,FPV-10,FPV-15,FPV-251,FPV-271 and ***,the substitution of Ala300Pro(A300P)in VP2 was detected for the frst time in feline-derived FPV(FPV-251).FPV-251 isolate,with this substitution in VP2 protein,exhibited stable proliferative capacity in Madin-Darby canine kidney(MDCK)cells and A72 ***-271 was selected as the FPV control isolate due to its single amino acid diference from VP2 protein of FPV-251 at position 300(FPV-271 has alanine,while FPV-251 has proline).After oral infection,both FPV-251 and FPV-271 isolates caused feline panleukopenia,which is characterized by clinical signs of ***,FPV-251 can infect dogs through the oral route and cause gastrointestinal(GI)symptoms with lesions in the intestine and mesenteric lymph nodes(MLNs)of infected *** is the frst report on the presence of an A300P substitution in VP2 protein of feline-derived ***,FPV isolate with a substitution of A300P at VP2 protein demonstrated

关键词： Feline panleukopenia virus FPV Dogs VP2 gene characteristic Host range