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杉木扦插苗生长规律与成苗质量的研究

华中农业大学学报 1989年第3期8卷 254-258页

作者：崔满志杨昌岩沈宝仙华中农业大学林学系湖南省通道县林科所

根据试验结果,对杉木实生苗、萌条扦插苗和苗梢扦插苗的生长过程和质量进行了分析。结果表明:扦插苗的年生长节律与实生苗基本一致,生长盛期为8—10月中旬。两类苗木的质量,除了平均高、冠根比和合格苗的百分率差异不显著外,其它各项指... 详细信息

根据试验结果,对杉木实生苗、萌条扦插苗和苗梢扦插苗的生长过程和质量进行了分析。结果表明:扦插苗的年生长节律与实生苗基本一致,生长盛期为8—10月中旬。两类苗木的质量,除了平均高、冠根比和合格苗的百分率差异不显著外,其它各项指标,扦插苗均显著或极显著地优于实生苗。两种扦插苗之间,除一级侧枝长度和Ⅰ级苗百分率差异达显著和极显著水平外,其它各项指标都无差异。

关键词：杉木扦插苗无性繁殖采穗圃

Positive Selection Drives Lactoferrin Evolution in Mammals

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Positive Selection Drives Lactoferrin Evolution in Mammals

Prediction of Protein Homo-Oligomer Types by Using IB1 Algorithm

第四届中国畜牧科技论坛

作者： Liang-guoming,Jiang-xunping~* (Animal Science Department,huazhong agricultural university,Wuhan,430070 China)

＜正＞A maximum-likelihood method was used to estimate nonsynonymous-to-synonymous substitution ratios between lactoferrin（LF） genes and the results suggested that the evolution of LF gene was characterized by positive *** amino acid sites were identified and subjected to positive selection,among of those four sites were convergences located in the N1 *** force analysis revealed the constant pressure may be come from genetic arms *** studies had suggested that the positive charge and amphipathic antibacterial peptides of N1 domain play an important role in the anti-bacteria *** study reported that the positive selection sites were definite correlation with antimicrobial N1 domain and we postulate that the divergence in the N1 domain of LF proteins may result from rapid mutation to adapt relate bacteria,driven by positive *** research also supported the host-pathogen antagonism,as previously hypothesis that some sites involved in the interactions between transferrin and bacterial iron deprived proteins.

关键词： lactoferrin positive selection evolution force

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Wuhan university Journal of Natural Sciences 2011年第2期16卷 121-124页

作者： SONG Chaohong SHI Feng XIONG Huijuant College of Science Huazhong Agricultural University Wuhan430070 Hubei China

Protein homo-oligomers play an important role in various vital activities. Successful prediction of protein homo-oligomers directly from primary sequence is very beneficial to understand their protein function. In this paper, a total of 14 238 homo-oligomeric protein sequences are predicted by IB1 algorithm. 10-fold cross-validation test is applied to test the predictive capability of the proposed method. The predictive results show that overall prediction accuracy is 90.46%, which is at least 9% higher than that of previous results; furthermore,the sensitivity and Matthew＇s correlation coefficient for each class of homo-oligomers are also improved significantly. The results show that IB1 algorithm is effective and feasible,and very suitable for predicting protein homo-oligomer types.

关键词： homo-oligomers IB1 algorithm amino acids composition

Factors governing formation of todorokite at atmospheric pressure

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Science China Earth Sciences 2005年第10期48卷 1678-1689页

作者： CUI Haojie1, FENG Xionghan1, LIU Fan1, TAN Wenfeng1 & HE Jizheng2 1. College of Resources and Environment, huazhong agricultural university, Wuhan 430070, China 2. Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China huazhong agricultural university Chinese Academy of Sciences

Todorokite commonly occurs in Earth surface environments. The factors governing formation of todorokite, such as reaction temperature, metal ions, dissolved O2 and pH, were investigated in this paper. Results showed that the forming rate of todorokite and its crystallinity decreased with falling reaction temperature, and the effect of temperature was more significant than that of other parameters. Nature of metal ions in the interlayer of buserite precursor and the structure of the buserite precursor obviously affected buserite transformation into todorokite. Weak bonding between the metal ions and MnO6 layer of buserite was favorable to todorokite formation. The rate of todorokite formation was promoted at a lower temperature with appropriate bubbling of O2. The pH in the system slightly influenced the todorokite formation, and todorokite could also be formed in a weak alkali medium or in a slightly acidic medium. Aged buserite pre-cursor more easily form todorokite than the unaged one.

关键词： todorokite, buserite, manganese oxide mineral, atmospheric pressure, transformation.

Discovering Genes for Drought Resistance Improvement of Rice by Genetic and Genomic Approaches

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Discovering Genes for Drought Resistance Improvement of Rice...

Water Budget Analysis of Red Soils in Central Jiangxi Province, China

中国遗传学会植物遗传和基因组学专业委员会2009年学术研讨会

作者： Lizhong Xiong~* National Key Laboratory of Crop Genetic Improvement,huazhong agricultural university,Wuhan, 430070

Drought frequently occurs in late stages of growth and development of rice and causes significant yield *** have integrated approaches including germplasm screening,genetic analysis and marker-assisted selection,funct... 详细信息

关键词： Oryza abiotic stress screening transgenic testing T-DNA mutant

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Pedosphere 2004年第2期14卷 241-246页

作者： CHENJia-Zhou HEYuan-Qiu CHENMing-Liangt CollegeofResourcesandEnvironment HuazhongAgriculturalUniversityWuhan430070（China）.E-mail：whcjz@263net InstituteofSoilScience ChineseAcademyofSciencesNanjing210008（China）

The daily soil water budgets in the red soil areas of central Jiangxi Province,southern China,were investigated with a large-scale weighing lysimeter and runoff plots. From 1998 to 2000,peanuts (Arachis hypogaea L.) and rape (Brassica napus L.) were planted in the lysimeter and in 1999,peanuts were planted in the runoff plots. The soil water budget components including rainfall,runoff,percolation and evapotranspiration were measured directly or calculated by Richards' equation and water balance equation. The results showed that most rainfall,including rainstorms,occurred from March to July,and induced the greatest soil water percolation during the year. The evapotranspiration was still large from July to September when rainfall was minimal. Thus,the lack of synchronization in soil water inputs and losses was disadvantageous to crops growing in this region. Among the soil water losses,percolation was the largest,followed by evapotranspiration,and then soil runoff. Runoff was very small on farmland with crops. It was significantly different from the uncultivated uplands where large-scale runoff was usually reported. The soil water storage fluctuated sinusoidally,with a large amplitude in the rainy season and a small amplitude in the dry season.

关键词： red soil runoff plot water budget weighing lysimeter

Adsorption and Insecticidal Activity of Toxin from Bacillus thuringiensis on Rectorite

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Pedosphere 2007年第4期17卷 513-521页

作者： ZHOU Xue-Yong HUANG Qiao-Yun CAI Peng YU Zi-Niu Hubei Province Key Laboratory of Refractories and Ceramics Wuhan University of Science and Technology Wuhan 430081 （China）. College of Biotechnology and Chemical Engineering Tianjin University of Technology Tianjin 300191 （China） State Key Laboratory of agricultural Microbiology College of Resources and Environment Huazhong Agricultural Uni- versity Wuhan 430070 （China）

The adsorption and desorption of the toxin from Bacillus thuringiensis strain WG-001 on rectorite were studied at different toxin and/or rectorite concentrations, pH values and temperatures. The insecticidal activity of the adsorbed toxin was evaluated by determining the lethal concentration to kill 50% of the larvae of Heliothis armigera （LC50）. The adsorption of the toxin on rectorite in sodium carbonate buffer （pH 9） reached equilibrium within 0.5-1.0 h and the adsorption isotherm of the toxin followed the Langmuir equation （R^2 〉 0.99）. In the pH range from 9 to 11 （carbonate buffer）, the adsorbed toxin decreased with increasing pH. The adsorption amounts decreased with increasing rectorite：toxin ratio. The adsorption was not significantly affected by the temperature between 10 and 50 ℃. The X- ray diffraction analysis indicated occurrence of the intercalation of the rectorite by the toxin. The infrared absorption spectrum showed that the binding of the toxin did not alter its structure. The LC50 wlues of the adsorbed toxin were smaller than those of the free toxin. The rectorite protected the toxin from ultraviolet irradiation damage. The desorption of the adsorbed toxin in water ranged from 37.5% to 56.4% and from 27.4% to 41.8% in a carbonate buffer. The desorption percentage also decreased with increasing rectorite：toxin ratio.

关键词： Bacillus thuringiensis insecticidul uctivity rectorite toxin udsorption und desorption

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苏云金芽胞杆菌cryⅡ基因的克隆和表达

生物工程学报 1995年第2期11卷 167-172页

作者：韩明喻子牛华中农业大学农业部农业微生物重点开放实验室武汉430070

分离的苏云金芽胞杆菌(Bacillus thuringiensis)YBT-791对鳞翅目小菜蛾(Plutellaxylostella)有高毒力,将其质粒DNA提纯,经HindⅢ酶切后与杀虫晶体蛋白cryⅡ基因探针杂交,显示出分子量分别为5kb和9.4kb两条DNA阳性片段。把5kb的DNA阳性... 详细信息

分离的苏云金芽胞杆菌(Bacillus thuringiensis)YBT-791对鳞翅目小菜蛾(Plutellaxylostella)有高毒力,将其质粒DNA提纯,经HindⅢ酶切后与杀虫晶体蛋白cryⅡ基因探针杂交,显示出分子量分别为5kb和9.4kb两条DNA阳性片段。把5kb的DNA阳性片段克隆到pUC18的HindⅢ位点上并转化大肠杆菌TG1,经酶切和杂交检测,证明斑点杂交阳性克隆子中含有5kb的cryⅡ片段。把这个含cryⅡ基因的5kb HindⅢ片段进行亚克隆,将4kb的BamHI-PstI酶切片段插入穿梭载体pXI61中,并用电脉冲法克隆于苏云金杆菌不产伴胞晶体的突变株中,得到产生单一cryⅡ基因编码的杀虫晶体蛋白的克隆菌株M-5。经电镜观察,该克隆菌株能形成出发菌YBT-791多种形态伴胞晶体中的一种方形伴胞晶体;经免疫双扩试验,它只能与CryⅡ晶体蛋白抗血清形成沉淀线;经SDS-PAGE电泳,克隆菌的伴胞晶体只含有一种65kDa的晶体蛋白。生物测定结果表明它既对鳞翅目小菜蛾(Plutella xylostella)有毒性,又对双翅目致倦库蚊(Culex quinquefasciatus)有毒性。

关键词：苏云金芽胞杆菌 cryⅡ基因基因表达基因克隆

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发状病毒属病毒研究进展

武汉大学学报（理学版） 2003年第2期49卷 271-276页

作者：洪霓华中农业大学植物科技学院湖北武汉430072

发状病毒属 (Trichovirus)是 1993年建立的一个新的病毒属 ,现有成员 4个 ,苹果褪绿叶斑病毒 (A CLSV)为该属典型成员 .近年来 ,该属有关病毒的分子生物学研究取得很大进展 ,已完全或部分揭示了各成员病毒的基因组结构与功能 ,同时这些... 详细信息

发状病毒属 (Trichovirus)是 1993年建立的一个新的病毒属 ,现有成员 4个 ,苹果褪绿叶斑病毒 (A CLSV)为该属典型成员 .近年来 ,该属有关病毒的分子生物学研究取得很大进展 ,已完全或部分揭示了各成员病毒的基因组结构与功能 ,同时这些研究结果促进了病毒分类的完善 ,并使该属成员组成也发生了较大变化 .本文从分类地位的演变、基因组结构和功能。

关键词：发状病毒属研究进展分子生物学基因组结构病毒分类基因工程作物病害