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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 788-794页

作者： Hong-Tao XIE Ming-Gang LEI Yuan-Zhu XIONG* Chang-Yan DENG Si-Wen JIANG Bo ZUO Feng-E LI De-Quan XU Tao WANG Key Laboratory of Swine Genetics and Breeding,Ministry of Agriculture,huazhong agricultural university,Wuhan 430070,China Key Laboratory of Swine Genetics and Breeding Ministry of Agriculture Huazhong Agricultural UniversityWuhan 430070 China

In order to detect the molecular basis of heterosis in pigs,suppression subtractivehybridization was carried out to investigate the difference in gene expression in the Longissimus dorsimuscle tissues between Meishan×Yorkshire F1 crossbreeds and their parents,Meishan *** swinemyosin regulatory light chain 2 (MRLC2) gene differentially expressed between the crossbreeds and thepurebreds was isolated and identified using semi-quantitative reverse transcriptase polymerase chain reactionand its complete cDNA sequence was obtained using the rapid amplification of cDNA ends *** sequence of the gene is not homologous to any of the known porcine *** sequenceprediction analysis reveals that the open reading frame of this gene encodes a protein of 172 amino acidscontaining the putative conserved domain of the EF-hand *** predicted amino acid sequenceof porcine MRLC2 protein exhibits 99%,98%,98%,98% and 97% identity with that of cattle,human,dog,rat and mouse,*** homology analysis revealed that the MRLC2 protein was very muchconserved in *** tissue expression analysis indicated that the swine MRLC2 gene is highlyexpressed in muscle,fat,heart,liver,spleen,lung,kidney,stomach,small intestine,ovary and testis,but notexpressed in pancreas.

关键词： MRLC2 gene pig heterosis Longissimus dorsi suppression subtractive hybridization rapid amplification of cDNA end

Application of Green Fluorescent Protein Gene (gfp) in the Symbiosis between Mesorhizobium Huakuii and Astragalus Sinicus

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Science Foundation in China 2002年第2期10卷 41-44页

作者： Zhou Junchu Shi Qiaojuan Xie Bo(huazhong agricultural university, Key Laboratory of agricultural Microbiology, Wuhan 430070)

Green fluorescent protein (GFP) is a luminescent protein which was first discovered from A equoreavicto-ria[1]. The chromophore of wild type GFP consists of an imidazoione ring formed by cyclization of Ser65, dehydrat... 详细信息

关键词： green fluorescent protein gene (gfp) Mesorhizobiumhuakuii promoter-probe vector

Reproductive biology of Schizopygopsis younghusbandi Regan 1905(Cyprinidae:Schizothoracinae)in the middle reaches of Yarlung Tsangpo River,China

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Journal of Oceanology and Limnology 2018年第5期36卷 1825-1834页

作者： DUAN Youjian HUO Bin MA Baoshan YANG Xin XIE Congxin School of Fisheries and Life Science Dalian Ocean UniversityDalian 116023China College of Fisheries Huazhong Agricultural UniversityWuhan 430070China Yangtze River Fisheries Research Institute Chinese Academy of Uishery SciencesWuhan 430223China Hunan Fisheries Research Institute Changsha 410153China

The reproductive biology of Schizopygopsis younghusbandi(Cyprinidae: Schizothoracinae) was studied by 719 individuals that collected from August 2008 to August 2009 in the middle reaches of Yarlung Tsangpo River, China. We coupled monthly variations of the gonadosomatic index(GSI), monthly proportions of the macroscopic maturity stages with size distribution of oocytes to evaluate spawning seasons. Taken as a set, these results indicated that S. younghusband i spawned from March to April with high degree of the spawning synchronicity. The standard lengths and the ages at 50% maturity for male were 222 mm and 4.4 year, and 308 mm and 7.0 year for female. In addition, the estimated mean fecundity and mean relative fecundity were 18682 and 57.8 eggs per g body weight. The fecundity of S. younghusbandi increased linearly with increasing of standard length and body weight. This study provides details about the S. younghusbandi reproduction suggesting that may be this species is vulnerable to exploitation in the middle reaches of Yarlung Tsangpo River.

关键词： reproduction fecundity spawning season Tibet

用电超滤(EUF)法预测湖北省水稻土供钾状况的研究

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华中农业大学学报 1989年第S1期8卷 106-113页

作者：刘雄德华中农业大学土化系

用电超滤(EUF)法预测湖北省48种水稻土供钾状况的研究表明,EUF法0—35分钟解吸土壤钾量与耗竭盆栽试验水稻吸钾量、1mol NH_4OAC化学提取钾量之间都有极紧密相关,其直线和关系数(r)分别为0.7596和0.6576,均达到极显著水准。EUF法0—35... 详细信息

用电超滤(EUF)法预测湖北省48种水稻土供钾状况的研究表明,EUF法0—35分钟解吸土壤钾量与耗竭盆栽试验水稻吸钾量、1mol NH_4OAC化学提取钾量之间都有极紧密相关,其直线和关系数(r)分别为0.7596和0.6576,均达到极显著水准。EUF法0—35分钟解吸钾量与盆栽NP、NPK处理水稻干重百分比值(以NP/NPK×100%表示)之间也有紧密相关,其相关回归方程为y=41.60X^(0.1889),相关系数(r)=0.3415,达到显著水准.以EUF法的自5—10分钟解吸钾量(AB)与30—35分钟解吸钾量(CD)的AB/CD比值大小,反映着湖北省各地水稻土均有不同程度的固钾能力,以鄂南、鄂东南水稻土固钾能力相对较小,鄂北、鄂西北较大,这与其粘土矿物的组成、数量及土壤质地有关。利用EUF法可确定湖北省水稻土到达“最低水平”时交换钾的含量即“最低水平值”,这对恰当地评价1mol NH_4OAC法所测得的土壤钾量具有实际意义。原土壤以1 mol NH_4OAC法测得的钾量与土壤达到“最低水平值”时的差值(暂称可利用钾量)和EUF法测定值之间有紧密相关,其直线相关系数(r)为0.7340,达到极显著水准。

关键词： EUF 土壤钾电超滤水平值直线相关粘土矿物黄泥田稻株供试土样施钾

Effects of Phosphate Adsorption on Adsorption-Desorption and Availability of Cu and Zn Ions in Ultisols and Alfisols

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Pedosphere 2000年第4期10卷 355-362页

作者： LIXUEYUAN DONGYUANYAN 等 KeyLaboratoryofSubtropicalSoilResourcesandEnvironment ChineseMinistryofAgricultureHuazhongAgriculturalUniversityWuhan430070China KeyLaboratoryofSubtropicalSoilResourcesandEnvironment ChineseMinistr

Surface charge, secondary adsorption- desorption and form distribution of Cu2+ and Zn2+ in Ultisols and Alfisols having adsorbed phosphate were studied by potentiometric titration, adsorption equilibrium and sequential extraction method, respectively. The soil surface negative charges increased whereas the amount of positive charges decreased with increase of P adsorbed. The soil secondary adsorption capacity for Cu2+ and Zn2+ was positively significantly correlated with the amount of P adsorbed by the soils, which could be described by the Langmuir equation. The amounts of Cu2+ and Zn2+ desorption from soils were decreased after P adsorption by the soils and the relationship between them was linear. After the soils adsorbed P, form distribution of Cu2+ and Zn2+ in soils changed remarforbly.

关键词： adsorption-desorption Alfisol and Ultisol availability Cu2+ and Zn2+ phosphate adsorption

Incompatibility behavior of a symbiotic plasmid pMH7653Rb in Mesorhizobium huakuii 7653R

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Science China(Life Sciences) 2010年第6期53卷 738-742页

作者： HU GuoYuan1,LI WeiWei1 & ZHOU JunChu2 1Key Laboratory for Green Chemical Process of Ministry of Education,Wuhan Institute of Technology,Wuhan 430073,China 2State Key Laboratory of agricultural Microbiology,huazhong agricultural university,Wuhan 430070,China Key Laboratory for Green Chemical Process of Ministry of Education Wuhan Institute of Technology Wuhan China State Key Laboratory of agricultural Microbiology Huazhong Agricultural University Wuhan China

Mesorhizobium huakuii strain 7653R harbored two indigenous plasmids named pMH7653Ra and *** larger plasmid pMH7653Rb (symbiotic plasmid) was transferred to *** HN308SR harboring three plasmids: pMHHN308a,pMHHN308b and pMHHN308c,and HN3015SR harboring three plasmids: pMHHN3015a,pMHHN3015b and pMHHN3015c by tri-parent *** stable indigenous plasmids,pMHHN308b and pMHHN308c of HN308SR,were co-eliminated due to the introduction of pMH7653Rb,and the transconjugant was named *** results implied that pMH7653Rb and pMHHN308b,pMHHN308c were incompatible and might have been ascribed to the same incompatible *** plasmid profiles of transconjugant HN3015SRN14 showed that the second largest plasmid pMHHN3015b of HN3015SR was cured due to the introduction of *** results also implied that pMH7653Rb and pMHHN3015b were *** from plant nodulation tests showed that pMH7653Rb could only maintain the nodulation ability in transconjugant HN308SRN14 and its nodule number was more than that of wild strain HN308SR,but could not replace the nitrogen fixation effect of pMHHN308b and *** plasmid cured mutant HN308SRN14D harboring only pMHHN308a formed null nodules that demonstrated pMHHN308a was relevant to nodulation ***3015SRN14 harboring pMH7653Rb,pMHHN3015a and pMHHN3015c formed null nodules while HN3015SRN14D containing pMHHN3015a and pMHHN3015c lost the nodulation *** plasmid replication repC-like gene sequences were detected by a polymerase chain reaction from 7653R,HN308,HN3015,HN308SRN14 and *** repC gene sequence similarities of the strains tested attained 99%.

关键词： Astragalus sinicus conjugative plasmid transfer indigenous plasmid Mesorhizobium huakuii plasmid curing plasmid incompatibility Tn5-sacB

Influence of Formate on Bioactivity Material-thuringiensin Synthesized by Bacillus thuringiensis YBT-032

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Journal of Wuhan university of Technology(Materials Science) 2008年第5期23卷 691-693页

作者：王志喻子牛 College of Bioengineering Hubei University of Technology State Key Laboratoy of agricultural Microbiology National Engineering Research Center for Microbial Posticides Huazhong Agricultural University

The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1 · h and transformation ratio from glucose to thuringiensin increased by 43.30%.

关键词： bacillus thuringiensis precursor formate thuringiensin

Types　of　Crystalline　Iron　Oxides　and　Phosphate　Ad－sorption　in　Variable　Charge　Soils

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Pedosphere 1994年第1期4卷 35-46页

作者： LIU FAN XU FENG-LIN LI XUE-YUAN WANG YI-JUN and ZENG GUANG-QIAO(Department of Soil and Agrochemistry, huazhong agricultural university, Wuhan 430070 (China))

The types, contents and morphologies of crystalline Fe oxides and their relations to phosphate adsorptionon the clay fractions in soils with variable charge in southern China were investigated by means of XRD, TEM,EMA and chemical analysis *** indicated that the types and contents of crystalline Fe oxidesvaried with the soils examined. The dominant crystalline Fe oxide was hematite in the latosols and goethitesin the red *** yellow-brown soils, the only crystalline Fe oxide was *** difference between Aldand Alo came mainly from the Al substituting for Fe in the pe oxides. The crystal morphology of goethiteappeared mainly as subrounded flat or iso-dimensional rather than acicular particles. Hematites occurredin plates of various thickness. Their MCDa/MCDc ratios in the latosols and red soils were generally above1.5 and below 1.5, respectively. The MCD values of goethites and hematites were 15-25nm and 20-35nm,and their specific surface areas were 80-120m￣2/g and 35-75m￣2/g, *** goethite crystals weregenerally smaller. Variations of the total amounts of crystalline Fe oxi es in clay fractions were not related tophosphate adsorption. The types, contents and morphologies of crystalline Fe oxides in the soils remarkablyaffected phosphate adsorption characteristics of the soils. The phosphate adsorption of goethite was muchgreater than that of hematite. The higher the MCD /MCDc rotio of hematite, the lower the phosphateadsorption.

关键词： crystalline Fe oxides goethite hematite phosphate adsorption variable charge soils

ASYMPTOTIC STABILITY OF RAREFACTION WAVE FOR GENERALIZED BURGERS EQUATION

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Acta Mathematica Scientia 2005年第1期25卷 119-129页

作者：徐艳玲蒋咪娜 Department of Mathematics and Information Science Basic Sciences Huazhong Agricultural University Wuhan 430070 China Department of Mathematical and Statistical Sciences Central China Normal university Wuhan 430079 China

This paper is concerned with the stability of the rarefaction wave for the Burgers equationwhere 0 ≤ a < 1/4p (q is determined by (2.2)). Roughly speaking, under the assumption that u_ < u+, the authors prove the existence of the global smooth solution to the Cauchy problem (I), also find the solution u(x, t) to the Cauchy problem (I) satisfying sup |u(x, t) -uR(x/t)| → 0 as t → ∞, where uR(x/t) is the rarefaction wave of the non-viscous Burgersequation ut + f(u)x = 0 with Riemann initial data u(x, 0) =

关键词： Burgers equation rarefaction wave the method of successive approximation maximum principle a priori estimatc stability

Study on Molecular Chain Morphology of Konjac Glucomannan

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agricultural Sciences in China 2003年第7期2卷 798-803页

作者： LI Bin and XIE Bi-jun(Natural Product Chemistry Research Laboratory , Food Science Department, huazhong agricultural university , Wuhan 430070, P. R. China) Natural Product Chemistry Research Laboratory Food Science Department Huazhong Agricultural University Wuhan 430070 P. R. China

The long-range structure of konjac glucomannan(KGM)is studied by using laser light scatter(LLS), gel permeation chromatography(GPC)and method of viscosidity. The weight-average molecular weight(Mw), root-mean-square ratio of gyration[(S2)1/2], second viral coefficient(A2)and multi-dispersion coefficient(Mw/Mn)are 1.04×106, 105. 0±0. 9 nm,(-1. 59±0.28)×10-3 mol ml g-2 and 1.015±0.003, respectively. Mark-Houwink equation is established as [η] = 5. 96×10-2Mw0.73 and the molecular chain parameters are as follows: ML=982. 82 nm-1, Lp = 27. 93 nm, d = 0. 74 nm, h = 0. 26 nm, L = l 054.11 nm. Further more molecular chain morphology of KGM is studied by using atom force microscope(AFM)and transmission electronic microscope(TEM), and the result shows that the KGM molecular is an extending semi-flexible linear chain without branch. Therefore, the image of molecular chain morphology confirms the deduction drawn by Mark-Houwink equation and molecular chain parameters.

关键词： Konjac glucomannan Molecular chain parameter Molecular morphology Laser light scatter Atom force microscope