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阿仑膦酸钠联合金天格对泼尼松龙致斑马鱼骨丢失作用机制的研究

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今日药学 2018年第7期28卷 433-437页

作者：罗群吴铁易子琳罗世英王小平刘华珍蔡德广东医科大学广东湛江524023 湛江中心人民医院药剂科广东湛江524037 湛江中心人民医院保健科广东湛江524037 广东医科大学附属医院药学部广东湛江524000

目的探究阿仑膦酸钠(Aln)联合金天格对泼尼松龙诱导斑马鱼骨丢失的作用机制。方法将受精后3 d的斑马鱼胚胎随机分为空白对照组(0.5%DMSO)、模型组(25μmol·L^(-1)泼尼松龙)、Aln组、金天格组和Aln+金天格组,每组3份。9 d后处死,用茜素... 详细信息

目的探究阿仑膦酸钠(Aln)联合金天格对泼尼松龙诱导斑马鱼骨丢失的作用机制。方法将受精后3 d的斑马鱼胚胎随机分为空白对照组(0.5%DMSO)、模型组(25μmol·L^(-1)泼尼松龙)、Aln组、金天格组和Aln+金天格组,每组3份。9 d后处死,用茜素红染色,用于RNA分析、蛋白定量分析和形态学观察。结果与模型组、Aln组及金天格组比较,联用组的脊柱畸形弯曲率明显降低,Wnt的mRNA表达明显升高,RANKL、OC的mRNA表达明显下降,但对OPG的mRNA表达不如泼尼松龙组的影响显著;与两药单用组比较,Aln+金天格组Wnt-3a蛋白的表达量上升显著,而TNF-α、M-CSF和OSTF1蛋白的表达量降低明显。结论阿仑膦酸钠联合金天格对抗斑马鱼骨丢失的作用,可能与激活Wnt/β-catenin信号通路,进而影响RANKL/RANK/OPG系统,提高Wnt-3a蛋白的表达量,抑制RANKL和OC的mRNA表达,减少TNF-α、M-CSF和OSTF1蛋白的表达量有关,而两者对OPG的mRNA表达的影响需进一步研究。

关键词：阿仑膦酸钠金天格斑马鱼骨质疏松症作用机制

痰热清注射液联合胸腺五肽注射液治疗脑卒中并发肺部感染的疗效观察

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中国医院用药评价与分析 2016年第6期16卷 740-742页

作者：王小平郑小清罗群刘华珍周秋仁湛江市中心人民医院药剂科广东湛江524000

目的:探讨痰热清注射液联合胸腺五肽注射液治疗脑卒中并发肺部感染的疗效。方法:选取2014年9月—2015年9月收治的110例脑卒中并发肺部感染患者,采用随机数字表法分为对照组和观察组各55例。对照组患者给予痰热清注射液,观察组患者在对... 详细信息

目的:探讨痰热清注射液联合胸腺五肽注射液治疗脑卒中并发肺部感染的疗效。方法:选取2014年9月—2015年9月收治的110例脑卒中并发肺部感染患者,采用随机数字表法分为对照组和观察组各55例。对照组患者给予痰热清注射液,观察组患者在对照组治疗的基础上加用胸腺五肽注射液,观察2组患者的疗效、退热时间、咳嗽缓解时间、肺部啰音消失时间、白细胞计数恢复时间、血液生化指标恢复时间、住院时间、痰病原菌培养转阴及不良反应发生情况。结果:治疗后,观察组患者总有效率为94.55%(52/55),明显高于对照组的83.64%(46/55),差异有统计学意义(P<0.05);观察组患者的退热时间、咳嗽缓解时间、肺部啰音消失时间、白细胞计数恢复时间、血液生化指标恢复时间及住院时间均短于对照组,且痰病原菌培养转阴率明显高于对照组,差异均有统计学意义(P<0.05);观察组患者不良反应发生率为5.45%(3/55),明显低于对照组的12.73%(7/55),差异有统计学意义(P<0.05),2组患者均未见明显不良反应。结论:痰热清注射液联合胸腺五肽注射液治疗脑卒中并发肺部感染疗效确切,可明显改善患者的临床症状,且安全性好,值得临床推广。

关键词：痰热清注射液胸腺肽脑卒中肺部感染

Distribution Law of Goblet Cells in the Intestinal Tracts of African Ostrich Chicks

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Agricultural Science & Technology 2013年第1期14卷 103-106页

作者：金春艳彭克美刘婷婷王蕾曹维维宋卉刘华珍华中农业大学动物科技-动物医学学院湖北武汉430070

[Objective] This study aimed to investigate the distribution law of different goblet cells in the intestinal tracts of African ostrich chicks. [Method] Alcian blue/pe- riodic acid-schiff reaction （AB/PAS） was adopted to observe and analyze the types and distribution of goblet cells in the intestinal tracts of ostrich chicks. Acid mu- copolysaccharide could be stained blue with alcian blue （AB）, and neutral mu- copolysaccharide could be stained red with periodic acid-schiff reagent （PAS）. [Result] According to AB/PAS results, goblet cells in the intestinal tracts were divided in- to four types： TypeⅠ was pure red, with AB negative result and PAS positive result containing neutral mucoitin; type Ⅱ was pure blue, with AB positive result and PAS negative result containing acidic mucoitin; type Ⅲ was purple reddish, with PAS posi- tive result greater than AB; typeⅣ was purple blue, with AB positive result greater than PAS. Large quantities of goblet cells were found in the intestinal tracts of os- trich chicks, mostly type III and *** quantities of goblet cells were decreased gradually in the duodenum, jejunum and ileum, while the quantities were increased in the cecum, colon and rectum. The goblet cells in the large intestine are more than that in the small intestine. The most quantities of goblet cells were contained in rectum. [Conclusion] These results indicate that the distribution of goblet cells is closely related with the structure and function of intestinal tracts. The mucus secret- ed by the goblet Cells plays a series of important roles in the digestion and mucosal immunization.

关键词： AB/PAS Ostrich chicks Goblet cell Mucosal immunization

根皮苷对LO2正常人肝细胞体外细胞毒性的实验研究

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吉林中医药 2015年第11期35卷 1159-1161页

作者：陈署贤郭建茹朱冬梅刘华桢林继宗侯少贞中山大学附属第三医院肝病外科广州510630 澳门科技大学中药质量研究国家重点实验室澳门999078 广州中医药大学中药学院广州510006

目的探究根皮苷对正常人肝细胞LO2的体外细胞毒性。方法实验分为正常组和根皮苷组。将从甜茶中提取根皮苷配以不同浓度根皮苷的培养液,浓度分别为4.0、8.0、16.0、32.0、64.0、128、256、512、800、1 000μmol/L。分别在24、48和72 h,使... 详细信息

目的探究根皮苷对正常人肝细胞LO2的体外细胞毒性。方法实验分为正常组和根皮苷组。将从甜茶中提取根皮苷配以不同浓度根皮苷的培养液,浓度分别为4.0、8.0、16.0、32.0、64.0、128、256、512、800、1 000μmol/L。分别在24、48和72 h,使用MTT比色法测定并计算出细胞活力率。结果给药24、48和72 h后,根皮苷在4.0∽1 000μmol/L浓度的范围内对正常人肝细胞LO2无毒性作用,细胞的存活率在90%以上。结论在4.0∽1 000μmol/L浓度范围内,根皮苷对正常人肝细胞的正常增殖无明显抑制,未显示有肝细胞毒性作用。

关键词：根皮苷肝细胞 MTT

Histological Study on the Adrenal Gland of Zebra

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Agricultural Science & Technology 2013年第3期14卷 523-526页

作者：刘婷婷金春艳曹维维王蕾刘华珍宋卉彭克美华中农业大学动物医学院武汉430070

In order to explore the histological structure of zebra and to protect en- dangered species diversity, it is especially important to study the zebra adrenal his- tological structure. 2 zebra adrenals were studied by means of histological methods. The adrenal gland tissues were dissected into serial paraffin sections at the thick- ness of 3 μm. After HE and Masson staining, they were observed and photographed by the light microscope and Image-Pro Plus 6.0. The results show that zebra adrenal capsule is covered with thick connective tissue, which is about 230 μm. A large number of nerves and blood vessels are distributed in the capsule. The area of cortex is about 5 times larger than medullae. Zona glomerulosa is thin, about 300？m. The cells were arranged as column and ball. Zona fasciculata with the thickness of 1 750 μm is 6 times as thick as zona glomerulosa. The cells were ar- ranged in cord. Zona reticular with the thickness of 250 wm was closely adjacent to medulla and formed a jagged boundary. The medulla with the area of 4 mm2 was deeply dyed. It was concluded that some sympathetic ganglion cells were found in the medulla arranged in groups and surrounded with zona reticular cells but lots of the cells were found in zona reticular. Some connective tissue with chromaffin cells was found in the medulla center vein. The purpose of the present study provides reliable evidences for further study on morphology, physiology and pathology of the zebra.

关键词： Zebra Adrenal gland Zona glomerulosa Zona fasciculate Medulla

益气扶正复方提取物对小鼠免疫功能及荷瘤生存时间的影响

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吉林中医药 2015年第6期35卷 620-622页

作者：梁健梁楚燕吴亚运刘华桢朱冬梅侯少贞广州中医药大学中药学院广州510006

目的研究益气扶正复方提取物对小鼠免疫功能及荷瘤生存时间的影响。方法采用小鼠碳廓清法和鸡红细胞所致的血清溶血素抗体生成实验来观察益气扶正复方提取物对免疫的调节作用,通过检测S180腹水瘤小鼠生命延长率实验来测定药物对荷瘤小... 详细信息

目的研究益气扶正复方提取物对小鼠免疫功能及荷瘤生存时间的影响。方法采用小鼠碳廓清法和鸡红细胞所致的血清溶血素抗体生成实验来观察益气扶正复方提取物对免疫的调节作用,通过检测S180腹水瘤小鼠生命延长率实验来测定药物对荷瘤小鼠的影响。结果益气扶正复方提取物低、中、高剂量组的脾脏系数,廓清指数K的值均大于醋酸泼尼松模型组(P<0.05);益气扶正复方提取物低、中、高剂量组及青春宝组的血清溶血素水平的值均大于空白组(P<0.01);益气扶正复方提取物低、中、高剂量组及氟尿嘧啶组的生命延长率均大于空白组(P<0.01)。结论益气扶正复方提取物具有提高机体免疫力和抗肿瘤作用。

关键词：益气扶正免疫功能荷瘤小鼠

阿仑磷酸钠联合金天格治疗糖皮质激素性骨质疏松的临床观察

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今日药学 2015年第10期25卷 715-717,723页

作者：罗群蔡静月郑小清刘华珍王小平张华辉蔡德湛江中心人民医院药剂科广东湛江524037 湛江中心人民医院骨科广东湛江524037 广东医学院附属医院药学部广东湛江524001

目的探讨阿仑磷酸钠联合金天格胶囊治疗糖皮质激素性骨质疏松患者的临床疗效。方法采用随机对照试验设计,对46例糖皮质激素性骨质疏松患者分组,治疗组(n=23)使用阿仑磷酸钠片联合金天格胶囊,同时服用碳酸钙;对照组(n=23)给予骨化三醇和... 详细信息

目的探讨阿仑磷酸钠联合金天格胶囊治疗糖皮质激素性骨质疏松患者的临床疗效。方法采用随机对照试验设计,对46例糖皮质激素性骨质疏松患者分组,治疗组(n=23)使用阿仑磷酸钠片联合金天格胶囊,同时服用碳酸钙;对照组(n=23)给予骨化三醇和碳酸钙,连续治疗6个月,记录2组患者治疗前后的临床疗效、骨密度变化及碱性磷酸酶等数据。结果治疗6个月后,治疗组有效率高于对照组(P>0.05),治疗组患者的骨密度值增加明显(P<0.05),碱性磷酸酶下降显著(P<0.000 1)。结论阿仑磷酸钠联合金天格治疗糖皮质激素性骨质疏松的效果较好,能够有效改善临床症状,明显增加骨密度,安全性好,但长期疗效有待进一步研究。

关键词：阿仑膦酸钠金天格糖皮质激素性骨质疏松症临床疗效

Splicing Signals in the Human Hemoglobin Genes at the Sequence and Folding Levels

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Wuhan University Journal of Natural Sciences 2011年第2期16卷 93-100页

作者： ZHANG Wen XIE huazhen LI Qing ZHANG Lu liu Ciquan Modem Biology Research Center Yunnan University Kunming650091 Yunnan China Department of Cell Biology and Genetics Kunming MedicalCollege Kunming 650031 Yurman China Kunming Institute of Zoology Chinese Academy of SciencesKunming 650223 Yunnan China

Identification of the splice sites is a critical and tough issue in eukaryotic genome annotation. Here, a statistical study is introduced for detecting the splicing signals in the human hemoglobin （Hb） pre-mRNAs by using the approaches of regional pairwise alignment, splicing weight matrix scoring, and dynamic extended folding. First, the regional pairwise alignment results show that the coding regions of the human Hb genes are at a high level for both conservation and fluctuation. Second, the weighted matrix scoring results indicate that, although the authentic splicing motifs are always scored the highest in a sequence, the sequence motif alone is inadequate to precisely define the splice sites. Finally, we deduce the RNA frame structures by applying an extended folding approach to analyze the stable folding elements. We find out that the splice sequences tend to take stretching and partially paired conformations, which benefit recognition and competitive binding of the splicing factors. These results indicate that precise splicing is an integrated effect of multiple mechanisms of signal recognition at the level of sequence and structure.

关键词： splice site regional pairwise alignment splicing weight matrix dynamic extended folding

Dual-templating surface gel into thin SSZ-13 zeolite membrane for fast selective hydrogen separation

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Aggregate 2022年第6期3卷 157-165页

作者： huazhen Rong Yixing Guan Jun Li Ziyi Li Dan Ma Jingjing Zhang Terence Xiaoteng liu Xiaoqin Zou Faculty of Chemistry Northeast Normal UniversityChangchunChina School of Energy and Environmental Engineering University of Science and Technology BeijingBeijingChina Department of Mechanical and Construction Engineering Faculty of Engineering and EnvironmentNorthumbria UniversityNewcastle Upon TyneUK

Highly permeable zeolite membranes are desirable for fast gas separation in the *** the membrane’s thickness is deemed to be an optimal solution for permeability ***,we report the synthesis route of thin SSZ-13 zeolite membranes via the conversion of template-contained surface *** synthesis gel is fully crystallized into crack-free SSZ-13 membranes assisted with dual templates of N,N,N-trimethyl-1-adamantammonium hydroxide(TMAdaOH)and tetraethylammonium hydroxide(TEAOH).The specific functions of TMAdaOH for structure directing and TEAOH for crystallization regulating are well *** surface gel layer is impregnated onto porous alumina with subsequent crystallization into a 500 nm thick *** submicron-thick membrane exhibits high H_(2)permeance with 50×10^(-8)mol s^(-1)m^(-2)Pa^(-1)during hydrogen ***,the separation factors are retained around 23.0 and 31.5 for H_(2)/C_(2)H_(6)and H_(2)/C_(3)H_(8),*** approach offers a possibility for obtaining high-quality zeolite membranes for efficient hydrogen separation.

关键词： gas separation hydrogen selectivity zeolite membrane