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Expressing PHB synthetic genes through chloroplast genetic engineering

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Chinese Science Bulletin 2002年第16期47卷 1373-1376页

作者： ZHANG Jingyu SU Ning ZHANG Zhonglin zhao huayan ZHU Shengwei SONG Yanru Key Laboratory of Photosynthesis and Enviromental Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Chloroplast integration and expression vector containing expression cassettes for phbB, phbA, phbC and aadA genes was constructed and bombarded into the tobacco chloroplast genome. Transplastomic plants were analyzed with PCR and Southern blot. Their homoplastomy was also judged. Northern dot and RT-PCR analysis were employed to investigate transgene expression at transcriptional level. The results indicate that the chloroplast transformation system is compatible for poly-3-hydroxybutyrate (PHB) production.

关键词： chloroplast genetic engineering poly-3-hydroxybutyrate transplastomic tobacco transcriptional level gene silencing.

A Chromosome-level Genome Assembly of Wild Castor Provides New Insights into Its Adaptive Evolution in Tropical Desert

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Genomics, Proteomics & Bioinformatics 2022年第1期20卷 42-59页

作者： Jianjun Lu Cheng Pan Wei Fan Wanfei Liu huayan zhao Donghai Li Sen Wang Lianlian Hu Bing He Kun Qian Rui Qin Jue Ruan Qiang Lin Shiyou Lü Peng Cui CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical GardenChinese Academy of SciencesWuhan 430074China Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120China University of Chinese Academy of Sciences Beijing 100049China Sino-Africa Joint Research Center Chinese Academy of SciencesWuhan 430074China State Key Laboratory of Biocatalysis and Enzyme Engineering School of Life SciencesHubei UniversityWuhan 434200China

Wild castor grows in the high-altitude tropical desert of the African Plateau,a region known for high ultraviolet radiation,strong light,and extremely dry *** investigate the potential genetic basis of adaptation to both highland and tropical deserts,we generated a chromosome-level genome sequence assembly of the wild castor accession WT05,with a genome size of 316 Mb,a scaffold N50 of 31.93 Mb,and a contig N50 of 8.96 Mb,*** with cultivated castor and other Euphorbiaceae species,the wild castor exhibits positive selection and gene family expansion for genes involved in DNA repair,photosynthesis,and abiotic stress *** variations associated with positive selection were identified in several key genes,such as LIG1,DDB2,and RECG1,involved in nucleotide excision ***,a study of genomic diversity among wild and cultivated accessions revealed genomic regions containing selection signatures associated with the adaptation to extreme *** identification of the genes and alleles with selection signatures provides insights into the genetic mechanisms underlying the adaptation of wild castor to the high-altitude tropical desert and would facilitate direct improvement of modern castor varieties.

关键词： Ricinus communis L. Adaptive evolution Selection signature Genetic variation Genome assembly

High-static-low-dynamic stiffness isolator based on an electromagnetic negative stiffness spring with long linear stroke

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Science China(Technological Sciences) 2024年第3期67卷 740-752页

作者： YUAN ShuJin WU YaJun SONG DaiPing PU huayan MOU LiSheng HOU Lei zhao JingLei LI XuePing LUO Jun WU Jie HUANG XiaoXu State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China China Aerodynamics Research and Development Center Mianyang 621000China College of Materials Science and Engineering Chongqing UniversityChongqing 400044China

Negative stiffness mechanisms can improve low-frequency vibration isolation performance and have been widely used in the vibration isolation of precision equipment. However, the negative stiffness mechanism usually introduces a nonlinear stiffness,resulting in a nonlinear response and worsening the vibration isolation performance, especially under large amplitude *** this paper, an electromagnetic spring with linear negative stiffness(ESLNS) is proposed, in which the antagonistic ampere forces of the energized coils are used to generate negative stiffness within a long linear stroke. The magnetic field distribution is improved through the design of the magnetic circuit, thereby increasing the stiffness generation efficiency. The stiffness can be adjusted bidirectionally by current within the range of positive and negative stiffness. An electromagnetic stiffness model was established based on the equivalent magnetic circuit method. Experimental measurements verified the accuracy of the model and proved the linearity of the electromagnetic spring. A vibration isolator with high static and low dynamic stiffness(HSLDS) based on the ESLNS is designed and tested. The experimental results prove that the introduction of the ESLNS can effectively expand the isolation frequency band without changing the equilibrium position. Moreover, the vibration isolator with ESLNS does not produce nonlinear response. The proposed electromagnetic spring with linear negative stiffness extends the application range of HSLDS isolators to a large amplitude vibration environment.

关键词： linear negative stiffness mechanism low-frequency vibration isolation electromagnetic spring vibration control

民族自治区域省际边缘区团结协作公共空间的发展建构——基于渝东南民族地区的调研

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民族学刊 2024年第3期15卷 46-54,134页

作者：张华艳赵心宪重庆旅游职业学院马克思主义学院重庆409000 重庆第二师范学院重庆400067

民族自治区域省际边缘区团结协作公共空间的发展与建构是全面推进民族团结进步事业的重要方面。重庆渝东南民族地区是重庆市唯一集中连片的以土家族苗族为主的少数民族聚居区。调研发现,该区域团结协作公共空间的建构主要存在四方面问题... 详细信息

民族自治区域省际边缘区团结协作公共空间的发展与建构是全面推进民族团结进步事业的重要方面。重庆渝东南民族地区是重庆市唯一集中连片的以土家族苗族为主的少数民族聚居区。调研发现,该区域团结协作公共空间的建构主要存在四方面问题:共治共建理念不牢,资源整合度较低;共享意识不足,配合意识欠缺;“六联”机制不够深入,涉及面较窄;民众交往交流交融度不够,情感体验较弱。其原因有五个方面:区域顶层设计不足,缺乏具体政策和标准试点;公共空间协作政策不统一,基层实施有较大难度;考核评价机制缺失,干部和民众内生动力不足;品牌意识不强,团结协作缺乏精准目标;思想认识不足,未能形成社会合力。基于此,本文提出五个对策建议:加强区域顶层设计,创设精准目标;整合资源,打造品牌完善管理体系;加大宣传力度,提高思想认识;加强文化联谊活动,促进民族交往交流交融;加强校园教育,不断铸牢中华民族共同体意识。

关键词：民族自治区域省际边缘区团结协作公共空间重庆渝东南民族地区公共空间发展与建构

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The over-expression of AtTCP5 leads to the defected leaf development through suppressing cell proliferation and endoreduplication in Arabidopsis

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The over-expression of AtTCP5 leads to the defected leaf dev...

基于二氧化钒和狄拉克半金属的太赫兹传感器/滤波器设计

2006年拟南芥研究学术研讨会

作者： huayan zhao, Qing Tao, Dongxue Hu, Hongya Gu, Li-Jia Qu National Laboratory for Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China

＜正＞The TCP gene family plays important roles in leaf development. One of the family members in Arabidopsis, AtTCP5, shows high sequence similarity with CINCINNATA, a leaf curvature-related protein from snapdragon. Expression pattern analysis showed that AtTCP5, highly expressed in leaves, was expressed precisely at the onset of cell differentiation and expressed abundantly in the area that is

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光学学报 2024年第2期44卷 292-303页

作者：张璐陈哲孙超逸赵玉媛孙华燕许永平黄泽宇云南大学信息学院云南昆明650500

设计了一种基于二氧化钒和狄拉克半金属的滤波/传感太赫兹超表面。该超表面由基于狄拉克半金属的镜面对称双开口环以及具有矩形孔的二氧化钒薄膜构成。通过调控超表面中二氧化钒的电导率,可以实现传感器/滤波器两种功能的切换。结果显示... 详细信息

设计了一种基于二氧化钒和狄拉克半金属的滤波/传感太赫兹超表面。该超表面由基于狄拉克半金属的镜面对称双开口环以及具有矩形孔的二氧化钒薄膜构成。通过调控超表面中二氧化钒的电导率,可以实现传感器/滤波器两种功能的切换。结果显示:当二氧化钒处于绝缘态时,超表面工作在传感器模式,此时,增大样品厚度及样品与传感器的距离均可增大传感器的灵敏度;当二氧化钒处于金属态时,超表面工作在滤波器模式,滤波器在中心频率处的插入损耗为1.3 dB,回波损耗为12.7 dB。这种具有多功能的超表面在太赫兹传感器和滤波器等方面具有较高的应用价值。

关键词：光学器件太赫兹超表面多功能狄拉克半金属二氧化钒

基于五项核心指标的公立医院高质量发展路径探析

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中华医院管理杂志 2024年第1期40卷 7-11页

作者：陈浩夏小哲钱招昕周花燕赵红中南大学湘雅医院办公室长沙410008 中南大学湘雅医院医务部长沙410008 湖南省卫生健康委员会体制改革处长沙410008 中南大学湘雅二医院医务部长沙410011

高水平公立医院高质量发展试点目标确定的五项核心指标及目标值,即病例组合指数达到2左右,四级手术占比、技术服务性收入占医疗收入比例、人员支出占业务支出比例、人员薪酬中固定部分占比均达到60%,是评价公立医院发展质量和效益的核... 详细信息

高水平公立医院高质量发展试点目标确定的五项核心指标及目标值,即病例组合指数达到2左右,四级手术占比、技术服务性收入占医疗收入比例、人员支出占业务支出比例、人员薪酬中固定部分占比均达到60%,是评价公立医院发展质量和效益的核心指标。本研究从医院内部改革创新举措以及"三医"协同发展和治理的角度出发,结合某所高质量发展试点医院现状,逐一梳理针对五项核心指标的优化提升举措,并提出当前"三医"协同发展和治理推进公立医院高质量发展的发力点,包括落实政府投入责任、理顺医疗服务比价关系、深化医保支付方式改革、加快药品耗材集中采购扩面、促进前沿技术产品应用等,以期为深入推动公立医院高质量发展提供参考。

关键词：医院,公立高质量发展核心指标改革路径

不同人员辅助疏散模式下老年人照料设施设计研究

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城市建筑 2024年第2期21卷 177-180页

作者：吴岩喻华燕张鸽赵科科郑州大学建筑学院中国城市建设研究院有限公司

老年人照料设施中老年人大多自主行为能力弱,灾害发生时需要借助护理员的辅助才能完成疏散。本研究通过pathfinder软件对老年人照料设施护理员值班室位置的两种疏散设计进行仿真模拟,分别对比护理员分区辅助疏散和就近辅助疏散下老年人... 详细信息

老年人照料设施中老年人大多自主行为能力弱,灾害发生时需要借助护理员的辅助才能完成疏散。本研究通过pathfinder软件对老年人照料设施护理员值班室位置的两种疏散设计进行仿真模拟,分别对比护理员分区辅助疏散和就近辅助疏散下老年人疏散时间。研究表明:护理员分区辅助疏散时,在靠近被辅助老人位置增加护理员值班室,能减少护理员的实际辅助疏散距离,有效降低疏散时长;护理员就近辅助疏散时,辅助顺序是影响疏散效率的重要因素。

关键词：老年人照料设施辅助疏散仿真模拟

cDNA cloning and functional analysis of 4-coumarate ——CoA: ligase (4CL) gene in Chinese white aspen

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Progress in Natural Science:Materials International 2003年第12期 15-20页

作者： zhao huayan 1, WEI Jianhua 2, LU Jing 1, SONG Yanru 1*, WANG Tai 1 and ZHU Zhiqing 1(1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Beijing Center of Agro Biotechnology, Beijing Institute of Agriculture and Forestry, Beijing 100089, China) Key Laboratory of Photosynthesis and Environmental Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China Beijing Center of Agro-Biotechnology Beijing Institute of Agriculture and Forestry Beijing 100089 China

cDNA encoding 4 coumarate: CoA ligase (4CL) was isolated from the secondary developing xylem of Chinese white aspen ( Populus tomentosa ) by RT PCR for the first time, which was 1619 bp in length. The coding sequence and putative amino acid sequence showed 97.53% and 97.00% identity to that of Pt4CL1 in quaking aspen ( P. tremuloids ), respectively. Molecular analysis indicated that 4CL was encoded by multiple genes in *** , its mRNA was highly accumulated in xylem and the expression of 4CL revealed a biphasic pattern in one growing season, almost in phase with the expression of other related enzymes in lignin biosynthesis. Transgenic research showed that expression of antisense 4 CL cDNA led to the decreasing of lignin content in transgenic tobaccos, among which the average reduction was 10.3% and the highest could be up to 18.9%. These data suggested that 4 CL gene was a potential gene used in altering lignin biosynthesis by biotechnology for producing new materials of papermaking.

关键词： Populus tomentosa RT PCR 4CL antisense RNA lignin.