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Experimental study of the isothermal sorption properties of late Qing and 1980s grey bricks in Wujiang, Suzhou, China

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Frontiers of Architectural Research 2013年第4期2卷 483-487页

作者： Yonghui li huarong Xie Jianguo Wang Xinjian li School of Architecture Key Laboratory of Urban and Architectural Heritage Conservation of Ministry of Education Southeast University Nanjing 210096 China

Grey brick is a key material in Chinese traditional architecture. The isothermal sorption curve of grey bricks is one of the basic moisture characteristics for studying the heat transfer of brick building enclosure structures and is also a fundamental parameter of research into the degradation mechanisms of Chinese traditional architecture. In this study, we measure the isothermal sorption curves of Wujiang （Suzhou, China） bricks from the 1980s and the late Qing period, by applying constant relative humidity controlled by saturated salt-water solutions under isothermal conditions. We then present their fitting formulas for humidity bound ranges of 5-92%. The results indicate that samples of the same-area traditional Suzhou grey bricks from different time periods show relatively large differences in their isothermal sorption properties, with the isothermal sorption property values of the late Qing grey bricks about three times those of the 1980s grey bricks.

关键词： Grey brick Periods Equilibrium moisture content Isothermal sorption Suzhou

Multiplex genome editing targeting soybean with ultra-low anti-nutritive oligosaccharides

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The Crop Journal 2023年第3期11卷 825-831页

作者： Wenxin lin Huaqin Kuang Mengyan Bai Xiaomeng Jiang Pengfei Zhou Yinghua li Bo Chen huarong li Yuefeng Guan College of Resources and Environment Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Guangzhou Key Laboratory of Crop Gene EditingInnovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006GuangdongChina FAFU-UCR Joint Center for Horticultural Biology and Metabolomics Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina College of life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Bioinformatics Department School of Life Sciences and TechnologyTongji UniversityShanghai 200092China KingAgroot CropScience Co. Ltd.Qingdao 266500ShandongChina

Soybean is the primary source of plant protein for *** to the indigestibility of the raffinose family of oligosaccharides(RFO),raffinose and stachyose are considered anti-nutritive factors in soybean ***-RFO soybean cultivars are generated by mutagenesis of RFO biosynthesis genes,but the carbohydrate profiles invite further modification to lower *** study employed a pooled multiplex genome editing approach to target four seed-specifically expressed genes mediating RFO biosynthesis,encoding three raffinose synthases(RS2,RS3,and RS4)and one stachyose *** T1progeny,rs2/rs3 and rs4/sts homozygous double mutants and a rs2/rs3/rs4/sts quadruple mutant(rfo-4m)were *** rs2/rs3 mutant showed reduced raffinose and stachyose contents,but the rs4/sts mutant showed only reduced stachyose in *** RFO contents in the rfo-4m mutant were almost *** analysis showed that the mutation of four RFO biosynthesis genes led to a shift of metabolic profile in the seeds,including the accumulation of several oligosaccharides-related *** mutants could contribute to precision breeding of soybean cultivars for soy food production.

关键词： Soybean Genome editing Raffinose family of oligosaccharides Raffinose Stachyose Precision breeding

Programmed sequential cutting endows Cas9 versatile base substitution capability in plants

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Science China(life Sciences) 2021年第6期64卷 1025-1028页

作者： Wei Yang Wei Qi Yucai li Jiyao Wang Yanmin Luo Dehui Ding Sudong Mo Bo Chen Yu Lu huarong li linjian Jiang Key Laboratory of Pest Monitoring and Green Management Ministry of Agriculture and Rural AffairsDepartment of Plant PathologyCollege of Plant ProtectionChina Agricultural UniversityBeijing 100193China Qingdao Kingagroot Compounds Co.Ltd. Qingdao 266426China

Dear Editors,CRISPR/Cas9-mediated genome editing techniques have triggered a revolution in biology research(Jinek et al.,2012).Cas9 coupled with guide RNAs cuts DNA at precise positions,and the resulting double stranded breaks(DSB)were effectively repaired by non-homologous end joining(NHEJ)pathway in higher eukaryotic cells,including animals and plants.

关键词： substitution versatile base

Systemic RNAi in western corn rootworm, Diabrotica virgifera virgifera, does not involve transitive pathways

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Insect Science 2018年第1期25卷 45-56页

作者： huarong li Andrew J. Bowling Premchand Gandra Murugesan Rangasamy Heather E. Pence Robert E. McEwan Chitvan Khajuria Blair D. Siegfried Kenneth E. Narva Dow AgroSciences LLC 9330 Zionsville Rd. Indianapolis Indiana USA Department of Entomology University of Nebraska Lincoln Nebraska USA Monsanto Company Chesterfield Missouri USA Entomology and Nematology Department University of Florida Gainesville Florida USA

Western corn rootworm （WCR, Diabrotica virgifera virgifera LeConte） is highly sensitive to orally delivered double-stranded RNA （dsRNA）. RNAi in WCR is systemic and spreads throughout the insect body. This raises the question whether transitive RNAi is a mechanism that functions in WCR to amplify the RNAi response via production of secondary siRNA. Secondary siRNA production is achieved through RNA-dependent RNA polymerase （RdRP） activity in other eukaryotic organisms, but RdRP has not been identified in WCR and any other insects. This study visualized the spread of the RNAi- mediated knockdown of Dr v-ATPase C mRNA throughout the WCR gut and other tissues using high-sensitivity branched DNA in situ hybridization. Furthermore, we did not detect either secondary siRNA production or transitive RNAi in WCR through siRNA sequence profile analysis. Nucleotide mismatched sequences introduced into either the sense or antisense strand of v-ATPase C dsRNAs were maintained in siRNAs derived from WCR fed with the mismatched dsRNAs in a strand specific manner. The distribution of all siRNAs was restricted to within the original target sequence regions, which may indicate the lack of new dsRNA synthesis leading to production of secondary siRNA. Thus, the systemic spread of RNAi in WCR may be derived from the original dsRNA molecules taken up from the gut lumen. These results indicate that the initial dsRNA dose is important for a lethal systemic RNAi response in WCR and have implications in developing effective dsRNA traits to control WCR and in resistance management to prolong the durability of RNAi trait technology.

关键词： corn rootworm Diabrotica secondary siRNA production systemic RNAi transitive RNAi

Expressing a human RNA demethylase as an assister improves gene-editing efficiency in plants

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Molecular Plant 2024年第3期17卷 363-366页

作者： Mengyan Bai Wenxin lin Chunyan Peng Peizhe Song Huaqin Kuang Jieni lin Jieping Zhang Jiyao Wang Bo Chen huarong li Fanjiang Kong Guifang Jia Yuefeng Guan Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Innovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China College of Agronomy China Agricultural UniversityBeijing 100193China College of life Sciences Fujian Agriculture and Forestry UniversityFuzhouFujian 350002China Synthetic and Functional Biomolecules Center Bejing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Kingagroot Co. Ltd.QingdaoShandong 266000China Tsinghua University-Peking University Joint Center for life Sciences Bejing 100871China

Dear Editor,Despite myriad successful applications of gene editing in plant functional genomics research and precision breeding,many challenges persist around the efficiency of gene-editing tools for many plant *** instance,soybean(Glycine max)is a major crop providing oil and protein to human diets and feedstock,but its gene-editing efficiency remains relatively low(Bai et al.,2019).

关键词： editing breeding

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室内空间要素重要性影响因素的探究

西南交通大学学报 2022年第2期57卷 440-446,454页

作者：李华蓉李天童陈涛重庆交通大学土木工程学院重庆400074

随着经济技术的快速发展,室内空间越来越复杂,对室内位置服务的需求日益增多,作为位置服务信息载体的室内地图产品也越来越多.但是,目前大多室内地图呈现的内容多于用户需求.针对这一问题,提出了基于用户需求的室内空间要素重要性影响... 详细信息

随着经济技术的快速发展,室内空间越来越复杂,对室内位置服务的需求日益增多,作为位置服务信息载体的室内地图产品也越来越多.但是,目前大多室内地图呈现的内容多于用户需求.针对这一问题,提出了基于用户需求的室内空间要素重要性影响因素的探究方法.该方法利用空间认知理论进行不同室内空间的多组心理行为试验,分析组内、组间试验结果,提取用户需求共性,得到室内空间要素的影响因素;通过验证试验最终确定室内空间要素重要性影响因素为:要素知名度、空间位置分布、要素独特性和相对规模.

关键词：因素分析要素分类空间认知室内空间

室内POI显著度评价的Fisher判别法模型

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测绘学报 2023年第4期52卷 679-688页

作者：李华蓉郑嘉欣李天童重庆交通大学智慧城市学院重庆400074 重庆市规划和自然资源调查监测院重庆401120

随着社会经济的快速发展,大型建筑物内部结构越来越复杂,室内空间的位置服务愈显重要。地标作为室内空间中的基础要素,能够帮助人们在室内认清方向和定位。但是,现有的室内地标的选取方式大多牵引于室外准则,未考虑室内POI独有的特点,... 详细信息

随着社会经济的快速发展,大型建筑物内部结构越来越复杂,室内空间的位置服务愈显重要。地标作为室内空间中的基础要素,能够帮助人们在室内认清方向和定位。但是,现有的室内地标的选取方式大多牵引于室外准则,未考虑室内POI独有的特点,导致所选取出的地标与用户的认知不匹配。针对这一问题,本文在室内空间POI显著度影响因素探究试验基础上,采用Fisher判别法(FDA)对试验数据进行语义信息量化、构建模型、模型回判和交叉互判处理,确定了室内POI显著度评价模型,并用于室内地标的提取。结果表明,建立的判别模型对室内POI显著度的判别和分级效果显著,选取出的地标能够与用户认知结果相一致。

关键词：空间认知室内空间显著度模型 POI Fisher判别分析

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基于图段连通体的线符号提取

测绘信息与工程 2010年第6期35卷 46-48页

作者：李华蓉武汉大学资源与环境科学学院武汉市珞喻路129号430079 重庆交通学院土木建筑学院重庆市学府大道66号400074

研究了基于符号连通体特征差异的不精准推理方法,分析了基于行邻接图的点、线、面符号图段连通体的特征,并根据三种符号在几何特征、形状特征和拓扑关系上的差异,提出了七种易于表达的特征参数,基于特征参数实现了线符号的自动提取。

关键词：图段连通体地图识别几何特征形状特征拓扑关系

空心锌铬铁氧体/二氧化钛复合物的制备及去污性能

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高等学校化学学报 2016年第11期37卷 2050-2059页

作者：孙代红刘华荣李睿哲长江大学化学与环境工程学院荆州434020

以葡萄糖溶液为原料,通过水热法制备了分散性良好的碳微球;以碳微球为模板,应用化学沉淀法和热处理技术除去碳核获得空心铬锌铁氧体;用钛酸四丁酯作为钛源,在空心铁氧体表面包覆二氧化钛制备了锌铬铁氧体/二氧化钛二元复合物.采用现代... 详细信息

以葡萄糖溶液为原料,通过水热法制备了分散性良好的碳微球;以碳微球为模板,应用化学沉淀法和热处理技术除去碳核获得空心铬锌铁氧体;用钛酸四丁酯作为钛源,在空心铁氧体表面包覆二氧化钛制备了锌铬铁氧体/二氧化钛二元复合物.采用现代分析技术表征了制备样品的微观结构、形貌和电磁性能,并对其吸附和降解染料废水的性能进行了研究.结果表明,Zn Cr_(0.25)Fe_(1.75)O_4对染料废水具有较好的去污效果,对甲基橙和罗丹明B的去污效率分别为87%和83%;TiO_2对铁氧体的包覆能提高复合物对染料废水的光催化降解性能,其中m_(T/ZCF)=0.3的ZnCr_(0.25)Fe_(1.75)O_4/TiO_2复合物的去污效果最佳,几乎与纯二氧化钛相仿.

关键词：空心铁氧体二氧化钛光催化甲基橙罗丹明B