检索结果-南通市图书馆

Microbial treatment in chronic constipation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2018年第7期61卷 744-752页

作者： Linsheng Huang Qi Zhu Xiao Qu huanlong qin Department of General Surgery Shanghai Tenth People's Hospital Affiliated to Tongji UniversityShanghai 200072China Clinical Medical College of Shanghai Tenth People's Hospital of Anhui Medical University Shanghai 200072China

Chronic functional constipation is a kind of common intestinal disease that occurs in children, adults and elderly people. This disease not only causes great influence to physiological function, but also results in varying degrees of psychological barriers. At present, constipation treatments continue to rely on traditional methods such as purgative therapy and surgery. However, these approaches can disrupt intestinal function. Recent research between intestinal diseases and gut microbiota has gradually revealed a connection between constipation and intestinal flora disturbance, providing a theoretical basis for microbial treatment in chronic constipation. Microbial treatment mainly includes probiotic preparations such as probiotics, prebiotics, synbiotics and fecal microbiota transplantation(FMT). Due to its safety, convenience and curative effect, probiotic preparations have been widely accepted, especially gradually developed FMT with higher curative effects. Microbial treatment improves clinical symptoms, promotes the recovery of intestinal flora, and has no complications during the treatment process. Compared with traditional treatments, microbial treatment in chronic constipation has advantages, and is worthy of further promotion from clinical research to clinical application.

关键词： constipation probiotics prebiotics synbiotics FMT

Fecal microbiota transplantation: standardization or diversification?

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第12期62卷 1714-1716页

作者： Xueying Zhang Hongliang Tian Qiyi Chen huanlong qin Ning Li Intestinal Microenvironment Treatment Center Shanghai Tenth People’s Hospital Tongji University School of Medicine

Call for standardization of fecal microbiota transplantation(FMT)The gut microbiota plays an important role in human health and *** is often recognized in patients with diseases of the digestive and other systems(Blaser,2019;Li et al.,2018b;qin et al.,2018).FMT refers to the administration of fecal material containing distal gut microbiota from a healthy person(donor)to a patient(recipient)to restore the gut microecology of the recipient(Kelly et al.,2015).Clinical guidelines have strongly recommended FMT for the treatment of relapsed or refractory Clostridium difficile infection(CDI)(Mullish et al.,2018).FMT has also shown potential clinical benefits in many other diseases related to dysbiosis,including inflammatory bowel disease(IBD),irritable bowel syndrome(IBS),functional constipation(FC),liver diseases,metabolic syndrome,autism,and cancer(Huang et al.,2018;Li et al.,2018a;Zhang et al.,2018).Because of its broad curative potential,FMT has become a topic of great *** than 3,000 published articles have reported treatment of different diseases with FMT,and over 380 FMT trials have been registered on the *** website.

关键词： donor standardization registered

Dysbiosis of gut microbiota was closely associated with psoriasis

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第6期62卷 807-815页

作者： Linsheng Huang Renyuan Gao Ning Yu Yefei Zhu Yangfeng Ding huanlong qin Shanghai Clinical College Anhui Medical University Shanghai 200072 China Department of General Surgery Shanghai Tenth People's Hospital Affiliated to Tongji University Shanghai 200072 China Department of Dermatology Shanghai Dermatology Hospital Shanghai 200043 China

Psoriasis is an autoimmune disease and gut microbiota participate in the establishment of intestinal immunity. This study was performed to identify the fecal microbial composition of psoriasis patients, and investigated the influence of subgroup(type and severity) on the fecal microbial composition, and to define the key microbiota in the pathogenesis of psoriasis. Fecal samples from 35 psoriasis patients and 27 healthy controls were sequenced by 16 S rRNA and then analyzed by informatics methods. We found that the microbiota of the psoriasis group differed from that of the heathy group. The relative abundances of Firmicutes and Bacteroidetes were inverted at the phylum level, and 16 kinds of phylotype at the genus level were found with significant difference. No microbial diversity and composition alteration were observed among the four types of psoriasis. The microbiota of psoriasis patients in the severe state differs from those of psoriasis patients with more mild conditions and also the healthy controls. The veillonella in fecal microbiota showed a positive relationship with h-CRP in blood. This research proved that psoriasis patients have a significant disturbed microbiota profiles. Further study of psoriasis based on microbiota may provide novel insights into the pathogenesis of psoriasis and more evidence for the prevention and treatment of psoriasis.

关键词： dysbiosis gut microbiota psoriasis severity

Activation of autophagy by in situ Zn^(2+) chelation reaction for enhanced tumor chemoimmunotherapy

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第11期29卷 116-131页

作者： Yang Yang Yefei Zhu Kairuo Wang Yunqiu Miao Yuanyuan Zhang Jie Gao huanlong qin Yang Zhang Nanomedicine and Intestinal Microecology Research Center Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai200072PR China School of Medicine Shanghai UniversityShanghai200444PR China Changhai Clinical Research Unit Shanghai Changhai HospitalNaval Medical UniversityShanghai200433PR China

Chemotherapy can induce a robust T cell antitumor immune response by triggering immunogenic cell death(ICD),a process in which tumor cells convert from nonimmunogenic to immunogenic ***,the antitumor immune response of ICD remains limited due to the low immunogenicity of tumor cells and the immunosuppressive tumor *** autophagy is involved in activating tumor immunity,the synergistic role of autophagy in ICD remains elusive and ***,we report an autophagy amplification strategy using an ion-chelation reaction to augment chemoimmunotherapy in cancer treatments based on zinc ion(Zn^(2+))-doped,disulfiram(DSF)-loaded mesoporous silica nanoparticles(DSF@Zn-DMSNs).Upon pH-sensitive biodegradation of DSF@Zn-DMSNs,Zn2+and DSF are coreleased in the mildly acidic tumor microenvironment,leading to the formation of toxic Zn2+chelate through an in situ chelation ***,this chelate not only significantly stimulates cellular apoptosis and generates damage-associated molecular patterns(DAMPs)but also activates autophagy,which mediates the amplified release of DAMPs to enhance *** vivo results demonstrated that DSF@Zn-DMSNs exhibit strong therapeutic efficacy via in situ ion chelation and possess the ability to activate autophagy,thus enhancing immunotherapy by promoting the infiltration of T *** study provides a smart in situ chelation strategy with tumor microenvironment-responsive autophagy amplification to achieve high tumor chemoimmunotherapy efficacy and biosafety.

关键词： Chemoimmunotherapy Immunogenic cell death(ICD) Damage-associated molecular patterns(DAMPs) Autophagy In situ chelation

Alterations in intestinal microbiota of colorectal cancer patients receiving radical surgery combined with adjuvant CapeOx therapy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2019年第9期62卷 1178-1193页

作者： Cheng Kong Renyuan Gao Xuebing Yan Linsheng Huang Jide He Hao Li Jie You huanlong qin Department of General Surgery Shanghai Tenth People’s Hospital Affiliated to Tongji UniversityShanghai 200072China Research Institute of Intestinal Diseases Tongji University School of MedicineShanghai 200072China Department of Oncology Affiliated Hospital of Yangzhou UniversityYangzhou 225000China Department of Traditional Chinese Medicine Shanghai Ninth People’s HospitalShanghai 200011China

An intricate relationship exists and interactions occur between gut microbiota and colorectal cancer(CRC).Radical surgery combined with adjuvant chemotherapy(AC) serves as the mainstream therapeutic scheme for most CRC *** current research was conducted to assess the effect of surgery or chemotherapy on gut ***-three CRC patients who received radical surgery and AC were *** samples were collected preoperatively,postoperatively,and after the first to fifth cycles of postoperative *** microbial community of each sample was analyzed using high throughput 16S rRNA amplicon *** with preoperative samples,fecal samples collected postoperatively exhibited a significant decrease of obligate anaerobes,tumor-related bacteria,and butyric acid-producing ***,a significant increase of some conditional pathogens was *** addition,the AC regimen(CapeOx) was found to alter intestinal microbiota *** particular,several changes were observed after chemotherapy including an increase of pathogenic bacteria,the "rebound effect" of chemotherapy-adapted bacteria,the shift of lactate-utilizing microbiota from Veillonella to Butyricimonas and Butyricicoccus,as well as the decrease of *** radical surgery and CapeOx chemotherapy exert a non-negligible effect on the gut microbiota of CRC ***-based intervention may be beneficial for patients during postoperative clinical management.

关键词： surgery chemotherapy intestinal microbiota colorectal cancer

Ketogenic diet for human diseases:the underlying mechanisms and potential for clinical implementations

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第2期7卷 402-422页

作者： Huiyuan Zhu Dexi Bi Youhua Zhang Cheng Kong Jiahao Du Xiawei Wu qing Wei huanlong qin Department of Pathology Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina Research Institute of Intestinal Diseases Tongji University School of MedicineShanghaiChina Department of Gastrointestinal Surgery Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina Shanghai Clinical College Anhui Medical UniversityHefeiChina

The ketogenic diet(KD)is a high-fat,adequate-protein,and very-low-carbohydrate diet regimen that mimics the metabolism of the fasting state to induce the production of ketone *** KD has long been established as a remarkably successful dietary approach for the treatment of intractable epilepsy and has increasingly garnered research attention rapidly in the past decade,subject to emerging evidence of the promising therapeutic potential of the KD for various diseases,besides epilepsy,from obesity to *** this review,we summarize the experimental and/or clinical evidence of the efficacy and safety of the KD in different diseases,and discuss the possible mechanisms of action based on recent advances in understanding the influence of the KD at the cellular and molecular *** emphasize that the KD may function through multiple mechanisms,which remain to be further *** challenges and future directions for the clinical implementation of the KD in the treatment of a spectrum of diseases have been *** suggest that,with encouraging evidence of therapeutic effects and increasing insights into the mechanisms of action,randomized controlled trials should be conducted to elucidate a foundation for the clinical use of the KD.

关键词： diseases epilepsy clinical

Optimization of Hydrolysis Conditions for the Isolation of Angiotensin-I Converting Enzyme(ACE) Inhibitory Peptides from Rhopilema hispidum

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2018年第6期17卷 1458-1464页

作者： SUN Zhenliang qin huanlong CAO Duo YAN Xuebing LI Hao HUANG Linsheng QU Xiao KONG Cheng WANG Man Department of General Surgery Shanghai Tenth People's Hospital Tongji University School of Medicine Shanghai Jiaotong University Affiliated Sixth People's Hospital South Campus The College of Life Science Northwest University

To obtain the maximum angiotensin-I converting enzyme(ACE) inhibitory activity, the protein hydrolysis conditions of the jellyfish Rhopilema hispidum were optimized using response surface methodology(RSM). Trypsin was selected to produce R. hispidum protein hydrolysates(RPH) with ACE inhibitory activity. The optimal parameters for producing protein hydrolysates with the highest ACE inhibitory activity were as follows: hydrolysis time 5 h, hydrolysis temperature 50℃, and the enzyme-to-substrate ratio 6%. Under these conditions, the ACE inhibitory rate of RPH could reach 64.28% ± 5.72%. In addition, RPH contained high levels of Gly, Glu, Pro, Ala, Asp and Arg, with a molecular weight distribution range of 0.32–6.84 kDa. The following three novel ACE inhibitory peptides were isolated and identified: Ile-Gly-Glu-Thr-Gly-Pro, Gly-Ala-Thr-Gly-Pro-Ala-Gly-Tyr-Val and Gly-AlaPhe-Gly-Pro-Gly-Gly-Leu-Val-Gly-Arg-Pro. The IC_(50) values of the ACE inhibitory activity of these three purified peptides were 19.07, 27.42 and 31.26 μmol L^(-1), respectively. These results suggested that proteins and peptides isolated from R. hispidum could be utilized as antihypertensive functional food sources.

关键词： Rhopilema hispidum ACE inhibitory peptide response surface methodology protein hydrolysate

Enzyme-like biomimetic oral-agent enabling modulating gut microbiota and restoring redox homeostasis to treat inflammatory bowel disease

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2024年第5期35卷 167-180页

作者： Zhangpeng Shi Xiaohong Li Jufeng Chen Zideng Dai Yefei Zhu Tan Wu qing Liu huanlong qin Yang Zhang Hangrong Chen Nanotechnology and Intestinal Microecology Research Center Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai200072PR China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050PR China School of Pharmacy Anhui Medical UniversityHefei230032PR China

Reactive oxygen species(ROS),immune dysregulation-induced inflammatory outbreaks and microbial imbalance play critical roles in the development of inflammatory bowel disease(IBD).Herein,a novel enzyme-like biomimetic oral-agent ZnPBA@YCW has been developed,using yeast cell wall(YCW)as the outer shell and zinc-doped Prussian blue analogue(ZnPBA)nanozyme *** orally administered,the ZnPBA@YCW is able to adhere to Escherichia coli occupying the ecological niche in IBD and subsequently release the ZnPBA nanozyme for removal of ***,meanwhile exhibiting improved intestinal epithelial barrier ***,it is found that the ZnPBA nanozyme exhibits remarkable capability in restoring redox homeostasis by scavenging ROS and inhibiting NF-κB signaling *** importantly,the 16S ribosomal RNA gene sequencing results indicate that post-oral of ZnPBA@YCW can effectively regulate gut microbiota by enhancing the bacterial richness and diversity,significantly increasing the abundance of probiotics with anti-inflammatory phenotype while downgrading pathogenic *** to the same level as normal *** a novel nanomedicine provides a new idea for efficient treating those ROS-mediated diseases accompanying with flora disorders.

关键词： Inflammatory bowel disease Biomimetic oral-agent Gut microbiota modulation ROS-Inflammation Yeast cellular wall

Targeting TM4SF1 exhibits therapeutic potential via inhibition of cancer stem cells

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第11期7卷 4018-4021页

作者： Guang Chen Xiaofei She Yanxin Yin Junxian Ma Yaqun Gao Hua Gao huanlong qin Jianmin Fang Cancer Center and Research Institute of Intestinal Diseases Shanghai Tenth People’s HospitalSchool of Life Sciences and Technology and School of MedicineTongji UniversityShanghaiPR China Shanghai Key Laboratory of Signaling and Disease Research School of Life Sciences and TechnologyTongji UniversityShanghaiPR China Biomedical Research Center Tongji University Suzhou Institute and Department of NeurologyTongji HospitalTongji UniversityShanghaiPR China

Dear Editor,Cancer stem cells(CSCs)are thought to be responsible for cancer initiation,growth,recurrence,metastasis,and drug resistance.1 Therefore,targeting CSCs is an effective therapeutic approach for cancer.2 However,there are few CSC-specific targets with functional extracellular domains for the development of antibody drugs.3 Our published paper demonstrated that transmembrane 4 L six family member 1(TM4SF1)coupled discoidin domain receptor tyrosine kinase 1(DDR1)under collagen I stimulation activated JAK2-STAT3 *** noncanonical DDR1 signaling sustained the manifestation of CSC traits by inducing SOX2 and NANOG expression and drove multiorgan metastases.

关键词： drugs STAT3 metastasis