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Levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus and its clinical significance

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Levels of autophagy in T cells and B cell of patients with s...

第十届全国免疫学学术大会

作者： Qiaofen Lan Yanwen Chen huanjin liao Tong Xie Huafeng Liu Qingjun Pan Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College

Objective:To investigate levels of autophagy in T cells and B cell of patients with systemic lupus erythematosus(SLE)and its clinical ***:68 SLE patients withought treatment within 4 weeks were enrolled in this *** accessed the levels of autophagy in T and B cells of 23 healthy controls and 68 patients before and after treatment by flow cytometry,and analyzed their correlations with serum levels of C3 and anti-ds DNA antibodies,SLEDAI score,et ***:Before treatment,a significantly increased levels of LC3-II was observed in SLE patients than healthy controls,the active group(SLEDAI score≥10)was significantly higher than the stable group(SLEDAI scorers=0.289,P<0.05),and the levels of C3 were positively correlated the levels of LC3-II in B lymphocyte(rs=0.371,P<0.01).After treatment for five days,levels of autophagy in T lymphocytes of SLE patients with good prognosis(SLEDAI score decreased≥4)significantly decreased(P<0.05);Also,three days after treatment,levels of autophagy in B lymphocytes of SLE patients with good prognosis were increased ***:Levels of autophagy in T and B lymphocytes of SLE patients were abnormal compared to healthy controls,and these changes were associated with disease ***,these changes are expected to be as indicators of disease activity and potential therapeutic targets in SLE.

关键词： Systemic lupus erythematous T lymphocytes B lymphocytes Autophagy LC3-II

Role of basophils in the early onset of collagen-induced arthritis mice

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Role of basophils in the early onset of collagen-induced art...

第十届全国免疫学学术大会

作者： Qiaofen Lan Yanwen Chen huanjin liao Pei Tang Tong Xie Huafeng Liu Qingjun Pan Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College

Objective:To investigate the role of basophil in early onset of collagen-induced arthritis in ***:C57BL/6 mice was immunized with chicken collagen typeⅡto induce arthritis(CIA)mouse *** was depleted with MAR-1 antibody,isotype antibody as *** of arthritis index(AI)of mice were *** levels of antibody anti-typeⅡcollagen,IL-4,IL-6 and IFN-γwere detected by *** was analyzed by HE ***:CIA mouse model was successfully *** with the control group and MARR-1 isotype control group,basophiles depleted can significantly reduce arthritis index,decrease serum levels of antibody anti-typeⅡcollagen,IL-4,but not IL-6 and IFN-γ.Meanwhile,basophils depletion significantly improved appearance of the joints and its pathology ***:Basophil depletion can reduce early onset of collagen-induced arthritis in mice,and this may be related to IL-4 and autoantibody production.

关键词： Basophils collagen-induced arthritis anti-Ⅱ collagen antibody IL-4

Factors affecting the hyperuricemia in IgA nephropathy patients with renal function compensatory phase in western part of Guangdong province

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Factors affecting the hyperuricemia in IgA nephropathy patie...

第十届全国免疫学学术大会

作者： Yanwen Chen Qiaofen Lan huanjin liao Jie Lu Qiuhua Chen Tong Xie Huafeng Liu Qingjun Pan Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College

Objective:To analyze the risk factors of hyperuricemia occurrence in Ig A nephropathy patients with renal function compensatory phase in western part of Guangdong ***:The clinical and pathological data of 328 patients with Ig A nephropathy were selected to investigate the risk factors of ***:The incident of hyperuricemia in Ig A nephropathy was 38.4%.Univariate regression analysis suggested that the risk factors of hyperuricemia in patients with Ig A nephropathy were related to age,serum creatinine,blood urea nitrogen,serum triglyceride,serum total cholesterol,glomerulus sclerosis score,glomerular injury integral,tubular-interstitial injury score,renal small vessel injury score and Lee’s *** regression analysis showed that the independent risk factors for hyperuricemia in patients with Ig A nephropathy were serum triglyceride and renal small vessel injury ***:Among the multiple risk factors of hyperuricemia in Ig A nephropathy with renal function compensatory phase in western part of Guangdong province,the independent risk factors were serum triglyceride and renal small vessel injury *** obtaining therapy strategies with regional characteristics,also provide a reference for other Ig A nephropathy patients with renal function compensatory phase.

关键词： Ig A nephropathy hyperuricemia risk factor

Basophil detection in rheumatoid arthritis patients and its relationship with disease activity and the imbalance of Th1 responses

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Basophil detection in rheumatoid arthritis patients and its ...

第十届全国免疫学学术大会

作者： Yanwen Chen Qiaofen Lan huanjin liao Qiuhua Chen Pei Tang Tong Xie Jie Lu Huafeng Liu Qingjun Pan Institute of Nephrology Affiliated Hostiptal of Guangdong Medical College Department of Rheumatology and Immunology Affiliated Hospital of Guangdong Medical College

Objective:To investigate the activation state of peripheral basophils from RA patients and its mechanism,and the relationship between disease activity and imbalance of Th1/Th2 ***:In this study,flow cytometry was employed to test the ratio of the number of Th1 to Th2 cell and levels ofbasophil and its activation state in peripheral blood mononuclear cells(PBMC)cells from rheumatoid arthritis(RA).ELISA method was used to measure serum levels of IFN-γand *** Blot was employed to detect serum circulating immune complexes containing Ig E(Ig E-CIC).Results:The ratio of the number of Th1 to Th2 cell have a low linear correlation with the DAS28 score;Serum levels of IFN-γin patients with RA was significantly higher than the control group,and correlated with the disease activity,but not IL-4;Levels of basophils of RA patients was significantly reduced compared with the control group,and highly activated(high expression of CD203c and CD62L);The presence of high levels of Ig E-CIC in RA patients,but not in control ***:Peripheral basophil of RA patients was highly activated and significantly reduced,may migrate to secondary lymphoid tissues and sites of inflammation,and against the imbalance of Th1 response,which may provide new clues for the prevention and treatment of RA.

关键词： rheumatoid arthritis Basophil Th1 Th2

间接ELISA定量检测抗IgE自身抗体方法的建立

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中华检验医学杂志 2019年第9期42卷 782-786页

作者：尹悦党宏高闯彭霞梁玉婷廖焕金李莉上海交通大学附属第一人民医院检验科上海200080 上海交通大学附属第一人民医院皮肤科上海200080

目的建立一种间接ELISA法定量检测IgG型抗人IgE自身抗体,并初步评估慢性荨麻疹(CU)患者血清抗IgE含量。方法选取2018年1至12月于上海市第一人民医院首诊的75例CU患者及120名健康体检者,收集相应血清样本及临床资料。以人IgE包被微孔板,... 详细信息

目的建立一种间接ELISA法定量检测IgG型抗人IgE自身抗体,并初步评估慢性荨麻疹(CU)患者血清抗IgE含量。方法选取2018年1至12月于上海市第一人民医院首诊的75例CU患者及120名健康体检者,收集相应血清样本及临床资料。以人IgE包被微孔板,加入人源化抗人IgE标准品(奥马珠单抗)或待检血清,再加入辣根过氧化物酶(HRP)标记的抗人IgG,建立间接酶联免疫法,并进行条件优化及方法学评价。采用Mann-WhitneyU检验、卡方检验分析CU患者及健康人血清IgE水平,采用受试者工作曲线(ROC)分析抗IgE对CU的诊断效能。结果IgE包被量5.0×10^-4mg/ml,血清稀释度1∶300,酶标抗体稀释度1∶100000;标准曲线R2=0.996;批内及批间变异系数3.9%~7.5%、6.0%~8.2%;血红蛋白≤1.3g/L、三酰甘油≤4.6mmol/L、胆红素≤171μmol/L时,对检测结果无明显影响;低、高浓度样品回收率95.9%、108.4%;空白限5.8×10^-4mg/ml,检出限1.8×10^-3mg/ml,定量限2.0×10^-3mg/ml;线性范围约2.0×10^-3~354.4mg/ml;抗IgE达354.4mg/ml时未见钩状效应;血清4℃过夜、反复冻融6次后抗IgE水平保持稳定。CU患者血清抗IgE水平[19.0(1.9,58.6)mg/ml]显著高于健康人血清抗IgE水平[0.7(0.002,11.1)mg/ml],P<0.001;当抗IgE截断值定为15.3mg/ml时,CU患者阳性率为54.7%,显著高于健康对照(18.3%),P<0.001,AUC达0.736。结论成功建立血清抗IgE的间接ELISA法,CU患者血清中存在抗IgE自身抗体。

关键词：免疫球蛋白E 自身抗体酶联免疫吸附测定荨麻疹

慢性荨麻疹患者血清IgE和IgE受体及其自身抗体的表达及意义

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中华检验医学杂志 2020年第4期43卷 411-415页

作者：尹悦高闯党宏许雯彭霞梁玉婷廖焕金李莉上海交通大学附属第一人民医院检验科上海200080 上海交通大学附属第一人民医院皮肤科上海200080

目的:探讨慢性荨麻疹(CU)患者血清免疫球蛋白E(IgE)、可溶性IgE高亲和力受体α(sFcεRⅠα)、IgG型抗IgE、IgG型抗FcεRⅠ自身抗体的水平及意义。方法:收集2018年1至7月于上海市第一人民医院首诊的43例CU患者作为CU组,同时选择37例皮炎... 详细信息

目的:探讨慢性荨麻疹(CU)患者血清免疫球蛋白E(IgE)、可溶性IgE高亲和力受体α(sFcεRⅠα)、IgG型抗IgE、IgG型抗FcεRⅠ自身抗体的水平及意义。方法:收集2018年1至7月于上海市第一人民医院首诊的43例CU患者作为CU组,同时选择37例皮炎湿疹患者及51名健康体检者分别作为疾病对照(DC)组及健康对照(NC)组。采用免疫比浊法检测血清IgE,酶联免疫吸附试验(ELISA)检测抗IgE、抗FcεRⅠ和sFcεRⅠα。采用非参数检验分析各组指标水平的差异,Spearman相关分析评估各指标间的相关性及其与病情的关系,受试者工作特征曲线分析各指标诊断价值。结果:CU组血清IgE、抗IgE、抗FcεRⅠ水平显著高于NC组,中位数分别为65.70 IU/ml比17.10 IU/ml,χ^2=28.541,P=0.001;0.61比0.39,χ^2=27.408,P=0.001;0.64比0.51,χ^2=29.102,P0.05。IgE、抗IgE、抗FcεRⅠ对CU的诊断效能(AUC均=0.72)优于sFcεRⅠα(AUC=0.61),四项联合检测可达最高诊断效能(AUC=0.83);抗FcεRI对CU与DC具鉴别诊断价值,AUC=0.70,P=0.002。结论:CU患者血清IgE、抗IgE及抗FcεRⅠ水平显著升高。肥大细胞活化相关分子具有成为CU诊断指标的潜能。

关键词：荨麻疹免疫球蛋白E 受体 IgE 自身抗体

p2PSA、p2PSA%、PHI在前列腺癌辅助诊断中的价值

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检验医学 2021年第7期36卷 705-709页

作者：权衡朱婧廖焕金伍均上海交通大学附属第一人民医院检验科上海200080 上海交通大学附属第一人民医院嘉定分院检验科上海201803

目的探讨前列腺特异性抗原同源异构体2(p2PSA)、前列腺特异性抗原同源异构体2百分比(p2PSA%)和前列腺健康指数(PHI)在鉴别良性、恶性前列腺疾病中的价值。方法选取行经直肠超声引导前列腺穿刺活检的男性患者250例,根据病理结果分为良性... 详细信息

目的探讨前列腺特异性抗原同源异构体2(p2PSA)、前列腺特异性抗原同源异构体2百分比(p2PSA%)和前列腺健康指数(PHI)在鉴别良性、恶性前列腺疾病中的价值。方法选取行经直肠超声引导前列腺穿刺活检的男性患者250例,根据病理结果分为良性前列腺疾病组(145例)和前列腺癌组(105例),将所有患者按年龄分为≤65岁和>65岁2个年龄段。检测所有患者血清总前列腺特异性抗原(t-PSA)、游离前列腺特异性抗原(f-PSA)和p2PSA,并计算游离前列腺特异性抗原百分比(f-PSA%)、p2PSA%和PHI。采用受试者工作特征(ROC)曲线评估各项指标诊断前列腺癌的效能。结果与良性前列腺疾病组比较,前列腺癌组t-PSA、p2PSA、p2PSA%、PHI均显著升高(P0.05)。ROC曲线分析结果显示,在≤65岁的患者中,t-PSA、f-PSA、f-PSA%、p2PSA、p2PSA%、PHI诊断前列腺癌的曲线下面积(AUC)分别为0.687、0.500、0.792、0.815、0.795、0.892;在>65岁的患者中,t-PSA、f-PSA、f-PSA%、p2PSA、p2PSA%、PHI诊断前列腺癌的AUC分别为0.748、0.542、0.900、0.780、0.843、0.929。结论前列腺癌标志物在不同年龄段中的诊断效能有一定差异。新型前列腺癌标志物PHI、p2PSA、p2PSA%可提高良性、恶性前列腺疾病的鉴别诊断效能。

关键词：前列腺特异性抗原同源异构体2 前列腺特异性抗原前列腺癌

AML患者异基因造血干细胞移植后复发危险因素分析

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检验医学 2023年第12期38卷 1160-1166页

作者：王洪玲刘梦娜白萍廖焕金上海交通大学医学院附属第一人民医院检验医学中心上海201600

目的探讨急性髓系白血病(AML)患者异基因造血干细胞移植(allo-HSCT)后复发的影响因素,为临床早期识别高危因素,及时采取干预措施提供依据。方法回顾性分析2017年1月1日—2020年12月31日上海交通大学医学院附属第一人民医院行allo-HSCT的... 详细信息

目的探讨急性髓系白血病(AML)患者异基因造血干细胞移植(allo-HSCT)后复发的影响因素,为临床早期识别高危因素,及时采取干预措施提供依据。方法回顾性分析2017年1月1日—2020年12月31日上海交通大学医学院附属第一人民医院行allo-HSCT的132例非M3型AML患者移植相关临床数据。采用Cox回归分析评价AML患者移植后复发和无复发生存的影响因素。结果132例患者中,复发25例,中位复发时间为5个月。复发组与非复发组TP53基因突变情况、移植前状态比较,差异有统计学意义(P<0.05)。多因素Cox回归分析结果显示,初诊白细胞计数≥100×10^(9)/L、TP53基因突变、移植前未缓解(NR)是AML患者移植后复发的独立危险因素(P<0.05)。Log-rank χ^(2)检验结果显示,初诊白细胞计数≥100×10^(9)/L组与白细胞计数<100×10^(9)/L组、TP53基因突变组与野生型组,移植前NR组与完全缓解(CR)组总无复发生存(RFS)率差异有统计学意义(P<0.05)。结论初诊时高白细胞计数、TP53基因突变、移植前NR可导致AML患者移植后复发风险增加。

关键词：急性髓系白血病异基因造血干细胞移植复发危险因素

RSV感染气液相界面培养人支气管上皮细胞模型的建立及其对HMGB1和pMLKL表达的影响

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检验医学 2022年第2期37卷 177-182页

作者：王娟廖焕金李延宁戈伊芹李佳上海交通大学附属第一人民医院检验医学中心上海200080

目的构建呼吸道合胞病毒(RSV)感染气液相界面(ALI)培养的人支气管上皮细胞(hBEC)模型,为深入研究呼吸道病毒致病机制提供更接近体内环境的细胞模型;通过分析RSV感染对高迁移率族蛋白1(HMGB1)和磷酸化混合系列蛋白激酶样结构域(pMLKL)表... 详细信息

目的构建呼吸道合胞病毒(RSV)感染气液相界面(ALI)培养的人支气管上皮细胞(hBEC)模型,为深入研究呼吸道病毒致病机制提供更接近体内环境的细胞模型;通过分析RSV感染对高迁移率族蛋白1(HMGB1)和磷酸化混合系列蛋白激酶样结构域(pMLKL)表达的影响,探讨RSV感染损伤支气管上皮的致病机制。方法将hBEC接种到Transwell膜上,进行液体浸没式培养,细胞汇合度达100%后转ALI培养,倒置显微镜观察细胞生长状态。细胞分化成熟后按照以下分组分别感染RSV病毒:6 h对照组,6 h感染复数(MOI)1.0组,6 h MOI 3.0组,24 h对照组,24 h MOI 1.0组,24 h MOI 3.0组。每组3个复孔。通过免疫荧光染色确认RSV感染效果和RSV感染对细胞核蛋白HMGB1和pMLKL表达的影响。结果经细胞扩展期液体浸没式培养4~10 d后,细胞汇合度达100%。转ALI培养约4周后,细胞条索状分布越来越清晰,且分泌肉眼可见的黏液,形成黏液层,将Transwell膜石蜡包埋切片,得到典型的假复层上皮HE染色结果。经抗RSV抗体免疫荧光染色确认,MOI为3.0感染24 h RSV病毒成功感染细胞;抗HMGB1和抗pMLKL免疫荧光双染色结果显示,该感染条件下,细胞核内出现粉色荧光[为蓝色4',6-二脒基-2-苯基吲哚(DAPI)和红色HMGB1荧光Merge结果],证实RSV感染后出现HMGB1核表达;而RSV感染前后均未见pMLKL表达。结论通过在Transwell膜上液体浸没式扩展培养和ALI分化培养,可获得分化良好的hBEC,且能较长时间维持其形态及功能,可为呼吸道病毒感染及其他常见呼吸道疾病研究提供更接近体内环境的细胞模型。在MOI 3.024 h条件下,RSV成功感染该细胞模型,并引起损伤相关分子蛋白HMGB1核表达。

关键词：人支气管上皮细胞气液相界面培养呼吸道合胞病毒高迁移率族蛋白1 磷酸化混合系列蛋白激酶样结构域