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Temporal characteristics of algae-denitrifying bacteria co-occurrence patterns and denitrifier assembly in epiphytic biofilms on submerged macrophytes in Caohai Lake,SW China

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Journal of Oceanology and Limnology 2023年第6期41卷 2276-2291页

作者： Pinhua XIA Guoqing LI xianfei huang Lei SHI Xin DU Tao LIN Guizhou Province Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment Guizhou Normal UniversityGuiyang 550001China

Denitrifying bacteria in epiphytic biofilms play a crucial role in nitrogen cycle in aquatic habitats.However,little is known about the connection between algae and denitrifying bacteria and their assembly processes in epiphytic biofilms.Epiphytic biofilms were collected from submerged macrophytes(Patamogeton lucens and Najas marina L.)in the Caohai Lake,Guizhou,SW China,from July to November 2020 to:(1)investigate the impact of abiotic and biotic variables on denitrifying bacterial communities;(2)investigate the temporal variation of the algae-denitrifying bacteria co-occurrence networks;and(3)determine the contribution of deterministic and stochastic processes to the formation of denitrifying bacterial communities.Abiotic and biotic factors influenced the variation in the denitrifying bacterial community,as shown in the Mantel test.The co-occurrence network analysis unveiled intricate interactions among algae to denitrifying bacteria.Denitrifying bacterial community co-occurrence network complexity(larger average degrees representing stronger network complexity)increased continuously from July to September and decreased in October before increasing in November.The co-occurrence network complexity of the algae and nirS-encoding denitrifying bacteria tended to increase from July to November.The co-occurrence network complexity of the algal and denitrifying bacterial communities was modified by ammonia nitrogen(NH_(4)^(+)-N)and total phosphorus(TP),pH,and water temperature(WT),according to the ordinary least-squares(OLS)model.The modified stochasticity ratio(MST)results reveal that deterministic selection dominated the assembly of denitrifying bacterial communities.The influence of environmental variables to denitrifying bacterial communities,as well as characteristics of algal-bacterial co-occurrence networks and the assembly process of denitrifying bacterial communities,were discovered in epiphytic biofilms in this study.The findings could aid in the appropriate understanding and use of epiphytic biofilms denitr

关键词： denitrifying bacteria epiphytic biofilms co-occurrence networks submerged macrophytes community assembly

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Studies on Organic Matter and Heavy Metals in Soils around the Coal-fired Power Plant in Jinsha

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Meteorological and Environmental Research 2017年第6期8卷 56-62页

作者： Cao Rensheng Fan Mirigyi huang xianfei Yang Hao Zhang Zedong Hu Jiwei Qin Farixin Guizhou Provincial Key Laboratory of Information System of Mountainous Areas and Protection of Ecological Environment Guizhou Normal UniversityGuiyang 550001China College of Forestry Guizhou UniversityGuiyang 550025China Institute of South China Karst Guizhou Normal UniversityGuiyang 550001China

The concentrations of organic matter and heavy metals( Hg,Cu,Pb,Zn,As and Cd) were analyzed in soils around the coal-fired power plant in Jinsha( Guizhou Province,China),and the ecological risks of these heavy metals were evaluated by using the ecological risk warning assessment. The results showed that the concentration of organic matter in soils was high with the average value up to 41. 54 g/kg,which met the threshold limit for the concentration of organic matter at level 1. The concentration of organic matter in the study area was above the level of lack or the level of urgency. The results from the correlation analysis showed that there were correlations between organic matter and speciation of the heavy metals( except Pb). The results from the ecological risk warning assessment showed that the order for the risk probability of individual ecological risk index above the level of no alarm was: Hg > Cu = Pb > Zn > As = Cd. The comprehensive indices revealed that the probability was 15. 62% for the sample locations at no alarm level,while the probabilities were 3. 13%,40. 63%,21. 87% and 18. 75% for sample locations at the levels of early warning,light warning,medium warning and serious warning,respectively. The results indicated that the ecological environment in this area has been adversely affected to some extent,and thus the treatment of heavy metals pollution should be strengthened in the future.

关键词： Heavy metals Organic matter Effect Warning assessment

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Estimation of soil organic carbon storage and its fractions in a small karst watershed

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Acta Geochimica 2018年第1期37卷 113-124页

作者： Zhenming Zhang Yunchao Zhou Shijie Wang xianfei huang Forest Resource and Environment Research Center of Guizhou Province College of Forestry Guizhou UniversityGuiyang 550025 China College of Forestry Guizhou University Guiyang 550025China Puding Karst Ecosystem Research Station of Guizhou Province Puding 562100 China State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of ScienceGuiyang 550002 China

With few available soil organic carbon(SOC)profiles and the heterogeneity of those that do exist, the estimation of SOC pools in karst areas is highly uncertain.Based on the spatial heterogeneity of SOC content of 23,536 samples in a karst watershed, a modified estimation method was determined for SOC storage that exclusively applies to karst areas. The method is a "soil-type method" based on revised calculation indexes for SOC storage. In the present study, the organic carbon contents of different soil types varied greatly, but generally decreased with increasing soil depth. The organic carbon content decreased nearly linearly to a depth of 0–50 cm and then varied at depths of 50–100 cm. Because of the large spatial variability in the karst area, we were able to determine that influences of the different indexes on the estimation of SOC storage decreased as follows: soil thickness > boulder content > rock fragment content > SOC content > bulk density. Using the modified formula, the SOC content in the Houzhai watershed in Puding was estimated to range from 3.53 to 5.44 kg m^(-2), with an average value of 1.24 kg m^(-2) to a depth of 20 cm, and from 4.44 to 14.50 kg m^(-2), with an average value of 12.12 kg m^(-2) to a depth of 100 cm. The total SOC content was estimated at 5.39*10^(5) t.

关键词： Bare rock rate Estimation method Soil organic carbon storage Small watershed Karst

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Application of chemical fractionation and X-ray powder diffraction to study phosphorus speciation in sediments from Lake Hongfeng,China

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Chinese Science Bulletin 2011年第20期56卷 2098-2108页

作者： JIANG CuiHong WU Di HU JiWei LIU Feng huang xianfei LI CunXiong JIN Mei Guizhou Provincial Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment Guizhou Normal UniversityGuiyang 550001China

Based on the techniques of sequential extraction and X-ray powder diffraction(XRD) ,the distribution characteristics of phosphorus speciation were investigated in 13 sediment samples from Lake Hongfeng,an important drinking water source for Guiyang with a typical karstic limestone environment.The total phosphorus(TP) concentrations in the sediments ranged from 973.36 to 2334.18 mg/kg with a mean concentration of 1635.87 mg/kg(dry weight) ,and it was evident that the phosphorus level in the sediments was high compared with other eutrophic lakes such as Lake Chaohu and Lake Xihu.The results of the two methods,sequential extraction and XRD,demonstrated the presence of different phosphorus species in the lake sediments.Being compatible with local geophysical characteristics,the results of sequential extraction techniques indicate that the average percentages of dissolved phosphorus(DP) ,aluminum-bound phosphorus(Al-P) ,iron-bound phosphorus(Fe-P) ,calcium-bound phosphorus(Ca-P) and organic phosphorus(OP) in the sediments were 0.40%,7.06%,5.07%,45.48%and 38.49%,respectively.Notably,the concentrations of inorganic phosphorus(IP) were higher than those of OP,and the IP consisted primarily of Ca-P.The high concentrations of Ca-P may temporarily control the release of phosphorus from the sediments because it is a relatively stable,inert and non-bioavailable phosphorus fraction.The results of XRD analysis indicated that,among phosphorus species,AlPO4 was found in all sediment samples at relatively high concentrations.Moreover,the TP concentrations in the sediments were high;thus the release potential and bioavailability of phosphorus in the sediments was important.Further statistical analyses of the results revealed significant correlations between phosphorus species in the sediments with two extractable principal components and four selectable cluster levels allowing interpretation of possible origins of phosphorus loading and transformation mechanisms of phosphorus species.Furthermore,available remediation measures were

关键词： X射线衍射分析湖泊沉积物粉末衍射法磷形态红枫湖化学分馏地球物理特征饮用水水源

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Application of City Wetland Park on Protection of Urban Rivers

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环境科学前沿（中英文版） 2015年第3期4卷 77-85页

作者： xianfei huang Jiwei Hu Fanxin Qin Guizhou Provincial Key Laboratory for Ecological Environment Guizhou Normal UniversityGuiyang 550001P.R.China College of forestry Guizhou UniversityGuiyang 550025P.R.China

With the rapid development of urbanization and the lack of sustainable regulation policies,large cities have become a critical pollution source to urban rivers.To elucidate the pollution status of the Nanming River,an urban river in Guiyang City,SW China,water samples were collected along the river from the upstream(through the urbanization area)to the downstream locations.Pollution levels of heavy metals,NH4+and total phosphorus(TP)were investigated,and dissolved organic matter(DOM)was analyzed with fluorescence spectrophotometer.The results reveal that huangjindadao,a local famous scenic spot,is a notable pollution source to the Nanming River,and the Huaxi National City Wetland Park plays an important role in protection of the water quality of this river.Analysis of 3DEEM characteristics of DOM indicated that the main pollution sources of DOM in this water body was municipal waste water.Protein-like compounds and humic acid with high molecular weight was the main components of DOM in this river.Organic compounds with low molecular weight represented a very small portion of the DOM in water of the Nanming River,especially in the downstream part of this river.

关键词： Pollution Heavy Metals Dissolved Organic Matter Urbanization Nanming River

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基于精细化管理的石油混炼造粒机寿命延长控制方法

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机械设计与制造工程 2022年第6期51卷 121-125页

作者：黄贤飞中韩(武汉)石油化工有限公司湖北武汉430082

在石油混炼造粒机挤出过程中,物料熔融的电阻加热器热惯性较大,导致能耗负荷过大,机器使用寿命降低,为此提出基于精细化管理的石油混炼造粒机寿命延长控制方法。根据物料熔融特性,探索造粒机运转过程中黏性耗散热、塑性能耗散以及摩擦... 详细信息

在石油混炼造粒机挤出过程中,物料熔融的电阻加热器热惯性较大,导致能耗负荷过大,机器使用寿命降低,为此提出基于精细化管理的石油混炼造粒机寿命延长控制方法。根据物料熔融特性,探索造粒机运转过程中黏性耗散热、塑性能耗散以及摩擦能耗散关联性,设计混炼造粒机机筒温控函数;令加热器加热形式为脉冲热流模式,设定机筒传热单元为矩形,简化温控算法,通过加热器断开与启动的预设温度,得到不同时刻下机筒径向温度分布情况;总结温度滞后与非线性影响因素,设计增强疲劳性的机筒温度控制策略。结果表明,该方法可有效控制机筒温度,能耗负荷处于平稳状态,从而延长造粒机使用寿命。

关键词：精细化管理石油混炼造粒机寿命延长物料熔融

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喀斯特山区土壤溶解性有机质光谱特征及来源解析

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环境工程 2023年第5期41卷 115-124页

作者：黄凯文马珍苫君月张珍明王兴富黄先飞贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室贵阳550001 贵州省生物研究所贵阳550008 贵州大学林学院/贵州省森林资源与环境研究中心贵阳550025

采用紫外-可见光谱(UV-vis)与三维荧光光谱(EEMs),结合平行因子分析(PARAFAC)方法,对贵州省典型喀斯特山区土壤溶解性有机质(SDOM)的特征、来源及组成进行分析。结果表明:土壤溶解性有机碳(SDOC)含量因土地利用类型不同而存在差异,其中... 详细信息

采用紫外-可见光谱(UV-vis)与三维荧光光谱(EEMs),结合平行因子分析(PARAFAC)方法,对贵州省典型喀斯特山区土壤溶解性有机质(SDOM)的特征、来源及组成进行分析。结果表明:土壤溶解性有机碳(SDOC)含量因土地利用类型不同而存在差异,其中草地表层SDOC含量显著高于林地。利用PARAFAC模型解析出3种荧光组分,C1(类腐殖质)、C2(类色氨酸)和C3(类酪氨酸),3种组分含量间存在显著正相关(P<0.01),且在不同土地利用方式下的分布有所差异。紫外特征参数显示:草地表层SDOM相对分子质量小于林地,旱地SDOM中芳香物质和疏水性组分较多。荧光特征参数显示:研究区SDOM总体腐殖化程度较低、稳定性较弱,且不同土地利用类型SDOM来源存在差异。林地以微生物源输入为主,旱地兼具微生物源与陆源共同作用,但以新生自生源为主。草地表层SDOM来源特征与旱地相同,亚表层则以微生物源输入为主。随着海拔升高,组分C1占比增加,C3占比降低,SDOM腐殖化程度增强,微生物源贡献减弱。SDOM荧光与吸光特征可有效表征喀斯特山地SDOM组分与来源特性,可作为区域土地利用与环境决策的评价指标。

关键词：土壤溶解性有机质喀斯特紫外-可见光谱三维荧光光谱平行因子分析

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贵州草海光合有效辐射衰减特征及影响因素研究

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人民长江 2023年第12期54卷 42-49页

作者：田源斌董继兴李安艳黄先飞夏品华贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室贵州贵阳550001 贵州草海湿地生态系统国家定位观测研究站贵州毕节553100

水下光环境对湖泊生态系统具有重要影响,是限制沉水植物生长、发育的重要因素,研究光合有效辐射衰减特征对湖泊沉水植物恢复具有重要指导意义。于2022年3~8月对草海水下光合有效辐射(PAR)时空分布进行调查,研究了光合有效辐射衰减系数(K... 详细信息

水下光环境对湖泊生态系统具有重要影响,是限制沉水植物生长、发育的重要因素,研究光合有效辐射衰减特征对湖泊沉水植物恢复具有重要指导意义。于2022年3~8月对草海水下光合有效辐射(PAR)时空分布进行调查,研究了光合有效辐射衰减系数(K d(PAR))与总悬浮物(TSS)、无机悬浮物(ISS)、有机悬浮物(OSS)、叶绿素a(Chl.a)和有色可溶性有机物(CDOM)等光衰减物质的关系,以及真光层深层(Z_(eu))的时空分布特征。结果表明:草海春夏季K_(d)(PAR)平均值为(2.90±1.12)m^(-1),Z_(eu)平均值为(1.84±0.69)m;光衰减因子TSS、ISS、OSS、Chl.a浓度和CDOM(380)吸收系数分别为(20.11±4.76)mg/L、(8.68±4.91)mg/L、(11.66±12.43)mg/L、(21.88±8.49)mg/m^(3)和(3.11±0.68)m^(-1);草海K_(d)(PAR)北部水域高于南部;草海水体K_(d)(PAR)与TSS(P<0.01)、ISS(P<0.01)和浊度(P<0.001)相关性显著,与Chl.a相关性不显著;春季影响草海K_(d)(PAR)的主导因子是ISS,夏季影响草海K_(d)(PAR)的因子是由ISS和CDOM共同主导,以春夏季整体来看,影响草海K_(d)(PAR)的因子是由ISS和Chl.a共同主导,这主要是由于草海水生植被衰退后风浪等导致沉积物再悬浮的缘故,且CDOM和Chl.a浓度的增加也是影响K_(d)(PAR)的关键因子;草海Z_(eu)平均值大于水深平均值,说明草海水体总体光环境有利于沉水植物生长发育,但是草海水体悬浮物质含量较高,其遮蔽可能导致沉水植物生长发育受阻,这也许是草海降低水位后沉水植物恢复不理想的一个重要原因。

关键词：光合有效辐射衰减系数沉水植物真光层深度悬浮物叶绿素a 草海

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黔西北茶园土壤活性铝的形态分布及影响因素

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环境科学研究 2015年第6期28卷 943-950页

作者：秦樊鑫魏朝富黄先飞姜鑫刘文政吴迪贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室贵州贵阳550001 西南大学资源环境学院重庆400716

土壤中各形态活性铝的质量分数决定了铝毒性的强弱,为了解茶园土壤中活性铝的形态分布及其影响因素,以采集于贵州西北部的106个茶园土壤样品为材料,采用化学连续提取法测定土壤中各形态活性铝的质量分数并分析其影响因素.结果表明:不同... 详细信息

土壤中各形态活性铝的质量分数决定了铝毒性的强弱,为了解茶园土壤中活性铝的形态分布及其影响因素,以采集于贵州西北部的106个茶园土壤样品为材料,采用化学连续提取法测定土壤中各形态活性铝的质量分数并分析其影响因素.结果表明:不同类型土壤中w(Tac-Al)(Tac-Al为活性铝)表现为黄壤>黄棕壤>棕壤>褐土;土壤中各形态活性铝质量分数分布略有差异,表现为w(Hy-Al)(Hy-Al为铝的水合氧化物与氢氧化物)>w(Ha-Al)(Ha-Al为腐殖酸螯合态铝)>w(Ex-Al)(Ex-Al为可交换态铝)>w(Or-Al)(Or-Al为有机结合态铝)>w(In-Al)(In-Al为无机吸附态铝),其中,结合较稳定的Hy-Al和Ha-Al占优势,二者质量分数之和在w(Tac-Al)中所占比例接近70.0%.相关性分析显示,土壤中各形态活性铝质量分数之间均呈极显著正相关(P<0.01),其中,w(Hy-Al)与w(Tac-Al)之间的R高达0.975(P<0.01),接近线性关系;w(Tac-Al)随着土壤p H的降低而升高,随着w(有机质)的增加而增大.因此,土壤中w(Tac-Al)并不由w(总Al)决定,而是受土壤p H和w(有机质)等因素的综合影响.

关键词：茶园土壤活性铝形态分布影响因素

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贵州草海湿地不同水位梯度沉积物中汞、砷形态分布及风险评价

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湖泊科学 2020年第1期32卷 100-110页

作者：汤向宸林陶夏品华黄先飞马莉杨羽贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室贵阳550001 贵州师范大学高原湿地生态与环境研究中心贵阳550001

通过改进Tessier连续提取法对贵州草海黑颈鹤栖息地不同水位梯度下沉积物汞(Hg)、砷(As)形态及生态风险进行了研究.结果表明,草海湿地沉积物中Hg含量在0.45~1.51 mg/kg之间,超过国家土壤环境质量农用地土壤风险管控标准;形态组成上,残... 详细信息

通过改进Tessier连续提取法对贵州草海黑颈鹤栖息地不同水位梯度下沉积物汞(Hg)、砷(As)形态及生态风险进行了研究.结果表明,草海湿地沉积物中Hg含量在0.45~1.51 mg/kg之间,超过国家土壤环境质量农用地土壤风险管控标准;形态组成上,残渣态汞(Res-Hg)>有机结合态汞(Org-Hg)>碳酸盐结合态(Car-Hg)>铁锰氧化态(FeO-Hg)>可交换态(Ex-Hg),不同水位梯度下含量和赋存形态在不同区域不一致.As含量在16.4~23.8 mg/kg之间,形态依次为残渣态砷(Res-As)>有机结合态砷(Org-As)>铁锰氧化态砷(FeO-As)>碳酸盐结合态砷(Car-As)>可交换态砷(Ex-As).As含量与贵州省土壤背景值持平,随着水位梯度的抬升,其总量呈增加趋势,残渣态占比逐步增多,性质逐渐稳定.采用地积累指数(I geo)、潜在生态风险指数(E i r)、风险评价编码法(RAC)对Hg、As的危害程度进行分析表明,基于草海较高Hg环境背景值,Hg整体污染风险较高,As处于低水平的污染风险等级且对环境影响较小.该研究揭示了不同水位梯度下Hg、As总量及形态分布特征,对草海湿地水位抬升恢复湿地提供了参考.

关键词：草海湿地水位梯度汞砷形态分布风险评价

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