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Circulating proteomic biomarkers for diagnosing sporadic amyotrophic lateral sclerosis:a cross-sectional study

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Neural Regeneration Research 2024年第8期19卷 1842-1848页

作者： Lu He Qinming Zhou Chaoyang Xiu Yaping Shao Dingding Shen Huanyu Meng Weidong Le Sheng chen Department of Neurology and Institute of Neurology Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Neurology Hospital of Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina Center for Translational Research on Neurological Diseases the First Affiliated HospitalDalian Medical UniversityDalianLiaoning ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Institute of Neurology Sichuan Academy of Medical Sciences-Sichuan Provincial HospitalChengduSichuan ProvinceChina Department of Neurology Xinrui HospitalWuxiJiangsu ProvinceChina

Biomarke rs are required for the early detection,prognosis prediction,and monitoring of amyotrophic lateral sclerosis,a progressive *** is an unbiased and quantitative method that can be used to detect neurochemical signatures to aid in the identification of candidate biomarke *** this study,we used a label-free quantitative proteomics approach to screen for substantially differentially regulated proteins in ten patients with sporadic amyotrophic lateral scle rosis compared with five healthy *** bstantial upregulation of serum proteins related to multiple functional clusters was observed in patients with spo radic amyotrophic lateral *** biomarke rs were selected based on functionality and expression *** validate the proteomics profiles,blood samples from an additional cohort comprising 100 patients with sporadic amyotrophic lateral sclerosis and 100 healthy controls were subjected to enzyme-linked immunosorbent *** substantially upregulated serum proteins in patients with spora dic amyotrophic lateral sclerosis were selected,of which the cathelicidin-related antimicrobial peptide demonstrated the best discriminative ability between patients with sporadic amyotrophic lateral sclerosis and healthy controls(area under the curve[AUC]=0.713,P<0.0001).To further enhance diagnostic accuracy,a multi-protein combined discriminant algorithm was developed incorporating five proteins(hemoglobin beta,cathelicidin-related antimicrobial peptide,talin-1,zyxin,and translationally-controlled tumor protein).The algo rithm achieved an AUC of 0.811 and a P-value of<0.0001,resulting in 79%sensitivity and 71%specificity for the diagnosis of sporadic amyotrophic lateral scle ***,the ability of candidate biomarkers to discriminate between early-stage amyotrophic lateral sclerosis patients and controls,as well as patients with different disease severities,was examined.A two-protein panel comprising talin-1 and transl

关键词： amyotrophic lateral sclerosis cathelicidin-related antimicrobial peptide hemoglobin label-free quantitative proteomics multi-protein combined diagnostic panel serum biomarkers talin-1 translationally-controlled tumor protein zyxin

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精子的体外优化技术

新乡医学院学报 2002年第6期19卷 513-516页

作者：王自然何全中新乡医学院第三附属医院生殖研究所河南新乡453003

目的　为临床精子的体外优化提供一种切实有效选择。方法　将近年来的精子优化技术加以综合分析。结果　临床上见到各种精液原因引起的不育如少精子、精子活力低下、畸形精子、精液液化不良等 ,针对这些原因有着不同的优化方法 ,各种优... 详细信息

目的　为临床精子的体外优化提供一种切实有效选择。方法　将近年来的精子优化技术加以综合分析。结果　临床上见到各种精液原因引起的不育如少精子、精子活力低下、畸形精子、精液液化不良等 ,针对这些原因有着不同的优化方法 ,各种优化方法有着优点和缺点 ,有时需要 2种或 2种以上的方法综合使用 ,才能达到更好的效果。结论　在临床优化技术的选择上一定要根据病人的具体情况 ,选择一种或两种或两种以上的优化方法 ,使优化效果更好。

关键词：精子不育体外优化

鸡源产气荚膜梭菌分离鉴定和药敏试验及枯草芽孢杆菌对其抑制作用

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动物医学进展 2025年第1期46卷 16-22页

作者：叶碧锦欧兰欣王定爱方肆云申翰钦严专强戚南山孙铭飞黄福强佛山科学技术学院广东佛山528225 广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业农村部禽流感等家禽重大疾病防控重点实验室广东广州510640 温氏食品集团股份有限公司广东新兴527400

为探究产气荚膜梭菌在黄羽肉鸡重要养殖区域的流行及耐药情况,采集广东、广西等华南地区,安徽、江苏、福建等华东地区6个规模化肉鸡养殖场9份肠道病变样品进行分离鉴定和毒素分型检测、耐药性分析,琼脂扩散试验检测野外分离枯草芽孢杆菌... 详细信息

为探究产气荚膜梭菌在黄羽肉鸡重要养殖区域的流行及耐药情况,采集广东、广西等华南地区,安徽、江苏、福建等华东地区6个规模化肉鸡养殖场9份肠道病变样品进行分离鉴定和毒素分型检测、耐药性分析,琼脂扩散试验检测野外分离枯草芽孢杆菌BSGZ7对产气荚膜梭菌的抑菌效果。结果显示,从肠道样品中分离到7株A型和2株G型产气荚膜梭菌,9株分离菌和1株标准菌均对阿莫西林、青霉素、头孢噻肟、氨苄西林、头孢氨苄、氟苯尼考、头孢曲松具有较高的敏感性,对庆大霉素、卡那霉素、四环素、多西环素、链霉素、磺胺异恶唑、林可霉素、红霉素、多黏菌素B等抗菌药物普遍耐药,枯草芽孢杆菌BSGZ7发酵液均对10株菌有抑菌效果。结果表明枯草芽孢杆菌对产气荚膜梭菌具有一定抑菌作用,有望成为防控鸡坏死性肠炎的新型绿色安全的抗菌制剂,同时也为临床上鸡坏死性肠炎的防控和治疗提供了参考依据。

关键词：产气荚膜梭菌分离鉴定药敏试验枯草芽孢杆菌抑菌活性

Review Article Electrocatalysts in lithium-sulfur batteries

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Nano Research 2023年第4期16卷 4438-4467页

作者： Shanying Wang Ziwei Wang Fangzheng chen Bo Peng Jie Xu Junzhe Li Yaohui Lv Qi Kang Ailin Xia Lianbo Ma Key Laboratory of Green Fabrication and Surface Technology of Advanced Metal Materials&Low-Carbon New Materials Research Center&School of Materials Science and Engineering Anhui University of TechnologyMaanshan 243002China Department of Polymer Science and Engineering Shanghai Key Laboratory of Electrical Insulation and Thermal AgeingShanghai Jiao Tong UniversityShanghai 200240China Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology(HKUST)Clear Water BayHong KongChina

Lithium-sulfur(Li-S)batteries with the merits of high theoretical capacity and high energy density have gained significant attention as the next-generation energy storage ***,the main pressing issues of sluggish reaction kinetics and severe shuttling of polysulfides hampered their practical *** overcome these obstacles,various strategies adopting high-efficient electrocatalysts have been explored to enable the rapid polysulfide conversions and thereby suppressing the polysulfide *** review first summarizes the recent progress on electrocatalysts involved in hosts,interlayers,and protective ***,these electrocatalysts in Li-S batteries are analyzed by listing representative works,from the viewpoints of design concepts,engineering strategies,working principles,and electrochemical ***,the remaining issues/challenges and future perspectives facing electrocatalysts are given and *** review may provide new guidance for the future construction of electrocatalysts and their further utilizations in high-performance Li-S batteries.

关键词： lithium-sulfur batteries slow reaction kinetics polysulfide shuttling electrocatalysts electrochemical energy storage

脐血间充质干细胞与骨髓间充质干细胞对淋巴细胞增殖的抑制效果比较

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中国组织工程研究与临床康复 2007年第24期11卷 4678-4681页

作者：贺文凤石庆之华建媛李剑李洁吴琼南昌大学医学院第二附属医院血液科江西省医学分子重点实验室江西省南昌市330006

目的:比较脐血间充质干细胞和骨髓间充质干细胞对植物血凝素诱导的淋巴细胞增殖的抑制作用。方法:实验于2005-07/2006-03在南昌大学第二附属医院血液病研究所及江西省医学分子重点实验室完成。①实验材料:脐带血9份,由南昌大学第二附属... 详细信息

目的:比较脐血间充质干细胞和骨髓间充质干细胞对植物血凝素诱导的淋巴细胞增殖的抑制作用。方法:实验于2005-07/2006-03在南昌大学第二附属医院血液病研究所及江西省医学分子重点实验室完成。①实验材料:脐带血9份,由南昌大学第二附属医院妇产科提供,产妇及其家属均知情同意。正常骨髓9份,由南昌大学第二附属医院血液科门诊及住院患者提供,均附有捐赠同意书。外周血9份,取自正常自愿者。本实验经医学伦理委员会批准。②实验方法:无菌条件下采集脐血、骨髓和正常人外周血,分离单个核细胞。脐血及骨髓单个核细胞以1×109 L-1接种于培养瓶中,加入含体积分数为0.1的小牛血清、1×10-3mol/L氢化可的松的IMDM培养液,7d后换液,去除悬浮细胞,以后每3～4d换液1次,待细胞90%融合后,胰蛋白酶+乙二胺四乙酸混合消化,传代培养,取第3代细胞用于实验。正常人外周血单个核细胞以1×109 L-1接种于培养瓶中,加入含体积分数为0.1的小牛血清、10mg/L植物血凝素的IMDM液,培养3d后收集细胞用于实验。③实验评估:采用流式细胞术检测脐血间充质干细胞及淋巴细胞表面分子。取第3代的脐血和骨髓间充质干细胞,体外诱导培养后以脂肪染色液鉴定其向脂肪细胞的分化情况。将不同细胞浓度的脐血和骨髓间充质干细胞分别加入到植物血凝素诱导的外周血淋巴细胞增殖体系中,比较两种不同来源的间充质干细胞对淋巴细胞增殖的调控作用。结果:①脐血间充质干细胞和骨髓间充质干细胞的培养及鉴定:第3代脐血间充质干细胞表面分子标记率CD29为85.18%,CD166为72.52%,CD54为33.70%,CD45为6.70%,CD13为1.51%,CD34为0.23%。正常人外周血单个核细胞中的CD3+T细胞为60%～70%,CD20+B细胞为3.5%～4.5%。脐血间充质干细胞诱导培养3周,可见呈桔黄色的脂肪细胞。②脐血间充质干细胞及骨髓间充质干细胞对淋巴细胞增殖的抑制:淋巴细胞:间充质干细胞为1∶1,1∶0.5,1∶0.1,1∶0.05,1∶0.02,1∶0.01时,脐血间充质干细胞对淋巴细胞增殖的抑制率分别为(49.5±4.8)%,(58.4±6.0)%,(38.1±4.0)%,(31.4±3.2)%,(24.3±3.2)%,(12.6±6.7)%,而骨髓间充质干细胞对淋巴细胞增殖的抑制率分别为(52.4±8.4)%,(65.1±9.7)%,(34.7±4.5)%,(13.0±6.4)%,(-10.7±12.6)%,(-43.9±9.4)%。后3种细胞浓度比例脐血间充质干细胞对淋巴细胞的抑制作用明显强于骨髓间充质干细胞(t=7.72,8.14,14.68,P均<0.05)。结论:脐血间充质干细胞与骨髓间充质干细胞一样,也具有抑制植物血凝素诱导淋巴细胞增殖的作用,抑制效果与间充质干细胞数量有关。低浓度的脐血间充质干细胞对淋巴细胞的抑制作用明显强于骨髓间充质干细胞。

关键词：骨髓脐血免疫抑制间充质干细胞

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不同胎龄胎心二尖瓣复合体的形态学观测

中国组织工程研究与临床康复 2007年第49期11卷 9921-9924页

作者：李思忠雒国胜申彪郭志坤王庆志商丘市医学高等专科学院解剖学教研室河南省商丘市476100 新乡医学院河南省新乡市453003

目的:二尖瓣复合体在结构和功能上是一个整体,它构成了左心室流入道主体。其任何一部分异常,均可导致二尖瓣功能障碍,引起先天性心脏病。观测各月龄胎儿心脏二尖瓣复合体的形态特征,分析其变化规律,为解剖学及临床诊治先天性心脏病积累... 详细信息

目的:二尖瓣复合体在结构和功能上是一个整体,它构成了左心室流入道主体。其任何一部分异常,均可导致二尖瓣功能障碍,引起先天性心脏病。观测各月龄胎儿心脏二尖瓣复合体的形态特征,分析其变化规律,为解剖学及临床诊治先天性心脏病积累资料。方法:①实验材料:选择2004-02/2005-12新乡地区非正常分娩的正常胎儿心脏40例,医院死亡鉴定小组确认胎儿已死亡,经死者家属同意捐献给医院处理,其中男21例,女19例,按胎儿发育特征及临床资料确定月龄,研究方案获伦理委员会批准。②实验方法:将40例心脏按不同月龄平均分为≤20周组,21～24周组,25～36周组,>36周组,每组10例,并记录胎儿的基本情况。③实验评估:解剖观测其二尖瓣复合体的形态特征。结果:纳入非正常分娩的正常胎儿心脏40例,均进入结果分析。①胎儿心脏二尖瓣复合体由二尖瓣瓣环、瓣膜、腱索和乳头肌组成。②由小到大4组的二尖瓣瓣环周长分别为(1.48±0.51)、(1.55±0.35)、(2.38±0.48)、(3.05±0.45)cm。前尖、后尖的高度分别为(0.33±0.13)、(0.20±0.03)cm;(0.34±0.10)、(0.21±0.04)cm;(0.51±0.15)、(0.28±0.01)cm;(0.62±0.01)、(0.39±0.10)cm。前、后乳头肌的最大高度分别为(0.46±1.13)、(0.44±0.19)cm;(0.52±0.10)、(0.50±0.11)cm;(0.65±0.17)、(0.70±0.12)cm;(0.73±0.10)、(0.74±0.13)cm。前、后乳头肌发出腱索的数目分别为(11.15±0.71)、(12.00±2.83)cm;(8.50±1.29)、(8.67±3.63)cm;(13.00±2.83)、(0.00±9.90)cm;(21.00±3.61)、(21.67±0.58)cm。结论:①乳头肌的增长变化与胎儿心脏整体增长变化是一致的,胎心部分结构随着胎龄增长可能发生重吸收。②胎儿心脏二尖瓣复合体的形态变化总体上与月龄变化呈正相关趋势。

关键词：二尖瓣复合体不同月龄胎心观测临床意义

Oxidation of norbornadiene:Theoretical investigation on H-atom abstraction and related radical decomposition reactions

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Propulsion and Power Research 2023年第1期12卷 104-113页

作者： Jintao chen Mingxia Liu Yuxiang Zhu Kairu Jin Zhenyu Tian Lijun Yang Chong-Wen Zhou School of Energy and Power Engineering Beihang UniversityBeijing 100191China Institute of Engineering Thermophysics Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China School of Astronautics Beihang UniversityBeijing 100191China Combustion Chemistry Centre School of Biological and Chemical SciencesUniversity of GalwayGalway H91TK33Ireland

The chemical kinetics of hydrogen atom(H-atom)abstraction reactions from norbornadiene(NBD)by five radicals(H,O(^(3)P),OH,CH_(3),and HO_(2)),and the unimolecular reactions of three NBD derived radicals,were studied through high-level ab-initio *** geometries optimization and vibrational frequencies calculation for all the reactants,transition states,and products were obtained at the M06-2X/6-311ttG(d,p)level of *** zeropoint energy(ZPE)corrected potential energy surfaces(PESs)were determined at the QCISD(T)/cc-pVDZ,TZ level of theory with basis set corrections from MP2/cc-pVDZ,TZ,qz methods for single point energy *** transition state theory(TST)was used for the rate constants calculations of H-atom abstraction reactions by five radicals(H,O(^(3)P),OH,CH_(3),and HO_(2))at temperatures from 298.15 to 2000 K,while the a-site H-atom abstraction reaction rate constant of NBD by OH radical has been obtained through variational transition state theory(VTST).The results show that the H-atom abstraction reactions from the α-carbon atom of NBD are the most critical channels at low *** rate constants for H-atom abstraction reactions by OH radical are also the fastest among all of the reaction channels investigated at the temperature range from 298.15 to 2000 ***-Ramsperger-Kassel-Marcus/Master Equation(RRKM/ME)has been used to calculate the pressure-and temperature-dependent rate constants for the unimolecular reactions of three related C7H7 product radicals which generated from H-atom abstraction reaction within temperature ranges of 300-2000 K and pressures of 0.01-100 atm.A combination of composite methods has been used to calculate the temperature-dependent thermochemical properties of NBD and related *** the calculated kinetics and thermochemistry data can be utilized in the model development for NBD oxidation.

关键词： Norbornadiene Ab-initio calculations Rate constants Kinetic Thermochemistry

Surface passivation by multifunctional carbon dots toward highly efficient and stable inverted perovskite solar cells

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Journal of Energy Chemistry 2023年第11期86卷 9-15,I0001页

作者： Qi Cao Yixin Zhang Xingyu Pu Junsong Zhao Tong Wang Kui Zhang Hui chen Xilai He Jiabao Yang cheng Zhang Xuanhua Li State Key Laboratory of Solidification Processing Center for Nano Energy MaterialsSchool of Materials Science and EngineeringNorthwestern Polytechnical UniversityXi'an 710072ShaanxiChina School of Chemistry and Chemical Engineering Anhui University of TechnologyMaanshan 243032AnhuiChina

Interfacial imperfections between the perovskite layer and the electron transport layer(ETL)in perovskite solar cells(PSCs)can lead to performance loss and negatively influence long-term operational ***,we introduce an interface engineering method to modify the interface between perovskite and ETL by using multifunctional carbon dots(CDs).C=O in the CDs can chelate with the uncoordinated Pb2+in the perovskite material,inhibit interfacial recombination,and enhance the performance and stability of *** addition,–OH in CDs forms hydrogen bonds with I-and organic cation in perovskite,inhibiting light-induced I2release and organic cation volatilization,causing irreversible degradation of perovskite films,thereby enhancing the long-term operational stability of ***,we achieve the champion inverted device with an efficiency of 24.02%.The CDs-treated PSCs exhibit high operational stability,and the maximum power point tracking only attenuates by 12.5%after 1000 *** modification engineering supported by multifunctional quantum dots can accelerate the road to stable PSCs.

关键词： Interfacial engineering Carbon dots Non-radiative recombination

Precipitation Behavior and Microstructural Evolution of Ferritic Ti–V–Mo Complex Microalloyed Steel

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Acta Metallurgica Sinica(English Letters) 2018年第9期31卷 997-1005页

作者： Ke Zhang Hui Wang Xin-Jun Sun Feng-Li Sui Zhao-Dong Li En-Xiang Pu Zheng-Hai Zhu Zhen-Yi huang Hong-Bo Pan Qi-Long Yong Key Laboratory of Metallurgical Emission Reduction and Resources Recycling Anhui University of TechnologyMinistry of EducationMaanshan 243032China School of Metallurgical Engineering Anhui University of TechnologyMaanshan 243032China Central Iron and Steel Research Institute Beijing 100081China

Precipitation behavior of （Ti, V, Mo）C and microstructural evolution of the ferritic Ti-V-Mo complex microalloyed steel were investigated through changing coiling temperature （CT）. It is demonstrated that the strength of the Ti-V-Mo microalloyed steel can be ascribed to the combination of grain refinement hardening and precipitation hardening. The variation of hardness （from 318 to 415 HV, then to 327 HV） with CT （from 500 to 600-625 ℃, then to 700 ℃） was attributed to the changes of volume fraction and particle size of （Ti, V, Mo）C precipitates. The optimum CT was considered as 600-625 ℃, at which the maximum hardness value （415 HV） can be obtained. It was found that the atomic ratios of Ti, V and Mo in （Ti, V, Mo）C carbides were changed as the CT increased. The precipitates with the size of 〈 10 nm were the V-rich particles at higher CT of 600 and 650 ℃, while the Ti-rich particles were observed at lower CT of 500 and 550 ℃. Theoretical calculations indicated that the maximum nucleation rate of （Ti, V, Mo）C in ferrite matrix occurred around 630 ℃, which was consistent with the 625 ℃ obtained from experiment results.

关键词： Nano-sized precipitates Precipitation hardening Ti-V-Mo Coiling temperature Ferrite