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Distribution characteristics and removal rate of antibiotics and antibiotic resistance genes in different treatment processes of two drinking water plants

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FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING 2024年第9期18卷 117-0页

作者： Wang, Jun huang, mingtao Li, Bolin Mohamed, Hassan Ibrahim Song, Huanjie Li, Gezi Yu, Ying Zhang, Han Xie, Weimin Wuhan Univ Technol Sch Resource & Environm Engn Wuhan 430070 Peoples R China Wuhan Lingang Econ & Technol Dev Zone Serv Ind Dev Wuhan 430040 Peoples R China Assiut Univ Fac Engn Civil Engn Dept Assiut 71516 Egypt Minist Ecol Environm Changjiang Basin Ecol & Environm Adm Changjiang Basin Ecol Environm & Monitoring & Sci Wuhan 430010 Peoples R China

Emerging pollutants, such as antibiotics and antibiotic-resistance genes, are becoming increasingly important sources of safety and health concerns. Drinking water safety, which is closely related to human health, should receive more attention than natural water body safety. However, minimal research has been performed on the efficacy of existing treatment processes in water treatment plants for the removal of antibiotics and antibiotic resistance genes. To address this research gap, this study detected and analyzed six main antibiotics and nine antibiotic resistance genes in the treatment processes of two drinking water plants in Wuhan. Samples were collected over three months and then detected and analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry and fluorescence quantitation. The total concentrations of antibiotics and antibiotic resistance genes in the influent water of the two water plants were characterized as December > March > June. The precipitation and filtration processes of the Zou Maling Water Plant and Yu Shidun Water Plant successfully removed the antibiotics. The ozone-activated carbon process increased the removal rate of most antibiotics to 100%. However, a large amount of antibiotic resistance gene residues remained in the effluents of the two water plants. The experiments demonstrated that the existing ozone-activated carbon processes could not effectively remove antibiotic resistance genes. This study provides a reference for the optimization of drinking water treatment processes for antibiotics and antibiotic resistance gene removal.

关键词： Antibiotics Antibiotics resistance genes Water treatment process unit Spatiotemporal distribution Removal efficacy QUINOLONE ANTIBIOTICS SULFONAMIDE RIVER SEDIMENT CHINA

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国内外科技伦理研究热点及可视化分析

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医学与哲学 2024年第7期45卷 24-29页

作者：黄明涛翟晓梅中国医学科学院/北京协和医学院群医学及公共卫生学院北京100730

对国内外科技伦理研究发展历程进行梳理,应用CiteSpace软件对关键词的聚类、时间线以及突现分析,总结国内外科技伦理研究热点大致分为科技伦理的理论研究、新兴科技及其伦理风险、科技伦理治理及规制三个部分,并对未来研究发展趋势进行... 详细信息

对国内外科技伦理研究发展历程进行梳理,应用CiteSpace软件对关键词的聚类、时间线以及突现分析,总结国内外科技伦理研究热点大致分为科技伦理的理论研究、新兴科技及其伦理风险、科技伦理治理及规制三个部分,并对未来研究发展趋势进行分析,更多的注意力集中到了影响人群健康和卫生保健制度的多种因素上。为促进国内开展科技伦理研究提供了不同的视角,有助于对科技伦理风险进行前瞻性预判,最终提升我国科技伦理治理能力,促进科技事业的健康有序发展。

关键词：科技伦理技术伦理伦理治理生命伦理学

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国际劳动教育研究的知识版图、论域与启示——基于WOS数据库核心文献的实证分析

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职业技术教育 2024年第19期45卷 73-80页

作者：黄明涛彭嫚丽莫玉音黔南民族师范学院马克思主义学院都匀558000 惠州学院广东省教育研究院

在党和国家高度重视劳动教育和劳动育人的背景下,探寻国际劳动教育研究的热点和发展趋势具有十分重要的意义。运用Citespace文献分析工具,对2000-2023年Web of Science数据库收录的1055篇有关劳动教育的核心文献进行实证分析发现,从200... 详细信息

在党和国家高度重视劳动教育和劳动育人的背景下,探寻国际劳动教育研究的热点和发展趋势具有十分重要的意义。运用Citespace文献分析工具,对2000-2023年Web of Science数据库收录的1055篇有关劳动教育的核心文献进行实证分析发现,从2009年起,国际劳动教育研究的文献迅速增长,2017年至今,年均发文量超过60篇。美国学者在该领域的发文量最高,英国、德国、中国、澳大利亚、西班牙和巴西也是劳动教育研究的重要国家。从发文期刊来看,《社会和行为科学》《教育经济学评论》《高等教育》和《应用经济学》等期刊发文量位居前列。研究热点主要包括劳动教育理念及相关政策、劳动教育与个体成长、劳动教育课程与师资建设、劳动教育与劳动力市场、劳动教育与经济社会之间的关系研究等方面。其中高被引论文的研究主题主要集中在劳动教育与劳动力市场之间的关系研究方面,说明了学术研究的实践导向。基于此,我国继续深化劳动教育研究的方向是:加强劳动教育的政策体系研究,加强对劳动教育实施效果和劳动力市场问题的研究,加强劳动课程体系和劳动师资问题的研究,加强劳动教育的实证研究。

关键词：劳动教育劳动育人劳动力市场

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出场、模仿与超越:中国式现代化的“他者”视野

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河南科技大学学报（社会科学版） 2024年第2期42卷 8-14页

作者：黄明涛余建军安徽大学马克思主义学院安徽合肥230601

中国式现代化需要置于与西方现代化这一“他者”比较的框架中去定位与理解。在发端处,西方现代化凭借其现代性霸权,吸引中国走上现代化道路来为建立合乎资产阶级利益需要的现代化秩序服务。同时,中国传统社会因开启现代化内源动力不足,... 详细信息

中国式现代化需要置于与西方现代化这一“他者”比较的框架中去定位与理解。在发端处,西方现代化凭借其现代性霸权,吸引中国走上现代化道路来为建立合乎资产阶级利益需要的现代化秩序服务。同时,中国传统社会因开启现代化内源动力不足,也需要“他者”的楔入才能正常开启现代化的历史进程。在发展中,中国现代化既有对资本主义现代化从器物、制度、思想层面的模仿以及对苏联现代化模式的模仿,也在模仿过程中对其是否为中国向何处去的答案进行反思。通过与西方现代化的深入比较,中国式现代化立足唯物史观,蕴含着独特的世界观、价值观、历史观、文明观、民主观和生态观。

关键词： “他者” 中国式现代化现代性资本主义现代化比较研究

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毛泽东农村调查研究中的群众路线意蕴、方法及其当代启示——以大革命与土地革命战争时期为中心

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山西高等学校社会科学学报 2024年第6期36卷 14-20页

作者：黄明涛余建军安徽大学马克思主义学院安徽合肥230601

大革命与土地革命战争时期是毛泽东开展农村调查研究的主要时期。他的农村调查研究,是在农民问题的重要性凸显、农民组织性需要加强、中国革命道路面临选择的历史背景下展开的。在农村调查研究中他坚持群众路线,解决了农民最关心的土地... 详细信息

大革命与土地革命战争时期是毛泽东开展农村调查研究的主要时期。他的农村调查研究,是在农民问题的重要性凸显、农民组织性需要加强、中国革命道路面临选择的历史背景下展开的。在农村调查研究中他坚持群众路线,解决了农民最关心的土地问题,找到了革命可以依靠的坚实力量,正确处理了农民阶级在革命中的阶级路线问题。毛泽东的农村调查研究方法系统、科学,既有矛盾分析法和阶级分析法两种基本方法,也有躬身实践法、座谈会调查法和数量分析法三种具体方法。新时代,回顾和总结毛泽东农村调查研究中的群众路线,有助于党员干部和调研工作者坚持问题导向,增强党和人民群众的血肉联系,改进调查方法,促进决策与调查研究结果的高度耦合。

关键词：毛泽东农村调查研究群众路线大革命与土地革命战争时期

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Advances in the dynamic control of metabolic pathways in Saccharomyces cerevisiae

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Engineering Microbiology 2023年第4期3卷 80-89页

作者： Chufan Xiao Yuyang Pan mingtao huang School of Food Science and Engineering South China University of TechnologyGuangzhou 510641China

The metabolic engineering of Saccharomyces cerevisiae has great potential for enhancing the production of high-value chemicals and recombinant *** studies have demonstrated the effectiveness of dynamic regu-lation as a strategy for optimizing metabolic flux and improving production *** this review,we provide an overview of recent advancements in the dynamic regulation of *** ***,we focused on the successful utilization of transcription factor(TF)-based biosensors within the dynamic regulatory network of *** biosensors are responsive to a wide range of endogenous and exogenous signals,including chemical inducers,light,temperature,cell density,intracellular metabolites,and ***,we ex-plored the potential of omics tools for the discovery of novel responsive promoters and their roles in fine-tuning metabolic *** also provide an outlook on the development trends in this field.

关键词： Dynamic control Response element Metabolic engineering Transcription regulation

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Identifying drivers of urban landuse changes in the Wenchuan earthquake- affected area by using night-time light data

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Journal of Mountain Science 2024年第4期21卷 1140-1159页

作者： huang Tao DING mingtao GENG Dongxian GAO Zemin ZHENG Hao School of Civil Engineering and Architecture Southwest University of Science and TechnologyMianyang 621010China Faculty of Geosciences and Environmental Engineering Southwest Jiaotong UniversityChengdu 611756China Analysis and Test Center of Sichuan Province Chengdu 610023China

To learn the process of urban land evolution before and after an earthquake is vital to formulate the urban reconstruction control policies and recovery measures in the earthquake-stricken ***,spatiotemporal evolution and its driving factors of urban land in earthquake-prone areas remains limited due to the scarcity of ground observation *** research,leveraging night-time light remote sensing imagery and land cover data,conducted a comprehensive analysis of the long-term evolution characteristics of urban land in earthquake-prone *** introduced methodologies for assessing the socio-economic impact and the primary natural environmental factors driving urban land evolution in these *** validate the proposed methods,the 2008 Wenchuan earthquake-affected area in China was selected as a representative study *** results indicated that the average Digital Number(DN)values in socio-economically impacted areas showed a trend of rising,falling,and then rising again after the *** values in three types of damaged areas including Type Ⅱ,Type Ⅲ,and Type Ⅳ exceeded pre-earthquake *** analysis of determinative factors influencing urban land evolution revealed that slope and elevation were key elements in controlling urban land expansion before the earthquake,whereas factors such as slope,elevation,lithology,and faults had a stronger influence on urban land expansion after the *** can be seen that,in view of the differences in the natural conditions of regions for post-disaster reconstruction,the local government need to actively adjust and adapt to urban spatial planning,so as to leverage the scale effect of large-scale inputs of funds,facilities,human resources and other factors after the disaster,thus enhancing resilience and recovery efficiency in response to disaster impacts.

关键词： Wenchuan earthquake Night-time lights Urban land Post-earthquake reconstruction

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Chromosomal Engineering of Escherichia coli for Efficient Production of Coenzyme Q_(10)

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Chinese Journal of Chemical Engineering 2014年第5期22卷 559-569页

作者：黄明涛陈韵妍刘建忠 Biotechnology Research Center and MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of Life Science Sun Yat-Sen University

The plasmid-expression system is routinely plagued by potential plasmid instability. Chromosomal integration is one powerful approach to overcome the problem. Herein we report a plasmid-free hyper-producer *** strain for coenzyme Q10 production. A series of integration expression vectors, pxKC3T5b and pxKT5b, were constructed for chemically inducible chromosomal evolution(multiple copy integration) and replicon-free and markerless chromosomal integration(single copy integration), respectively. A coenzyme Q10 hyper-producer Escherichia coli TBW20134 was constructed by applying chemically inducible chromosomal evolution,replicon-free and markerless chromosomal integration as well as deletion of menaquinone biosynthetic *** engineered E. coli TBW20134 produced 10.7 mg per gram of dry cell mass(DCM) of coenzyme Q10 when supplemented with 0.075 g·L-1of 4-hydroxy benzoic acid; this yield is unprecedented in E. coli and close to that of the commercial producer Agrobacterium tumefaciens. With this strain, the coenzyme Q10 production capacity was very stable after 30 sequential transfers and no antibiotics were required during the fermentation process. The strategy presented may be useful as a general approach for construction of stable production strains synthesizing natural products where various copy numbers for different genes are concerned.

关键词： coenzyme Q10 Escherichia coli chemically inducible chromosomal evolution replicon-free and markerless chromosomal integration chromosomal engineering

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Multiple Strategies for Metabolic Engineering of Escherichia coli for Efficient Production of Coenzyme Q_（10）

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Chinese Journal of Chemical Engineering 2011年第2期19卷 316-326页

作者：黄明涛王玥刘建忠毛宗万 The Key Laboratory of Gene Engineering of Ministry of Education State Key Laboratory of BiocontrolSun Yat-Sen UniversityGuangzhou 510275China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry Sun Yat-Sen UniversityGuangzhou 510275China

Escherichia coli BW25113 was metabolically engineered for CoQ10 production by replacing ispB with ddsA from Gluconobacter *** of precursor balance and reduced nicotinamide-adenine dinucleotide phosphate （NADPH） availability on CoQ10 production in *** were *** knockout of pykFA along with pck overexpression could maintain a balance between glyceraldehyde 3-phosphate and pyruvate,increasing CoQ10 *** of native NAD-dependent gapA with NADP-dependent gapC from Clostridium acetobutylicum,together with the overexpression of gapC,could increase NADPH availability and then enhanced CoQ10 *** effects,overexpressions of various genes in CoQ biosynthesis and central metabolism,different vectors and culture conditions on CoQ10 production in ***,were all *** investigation of different vectors indicated that low copy number vector may be more beneficial for CoQ10 production in *** recombinant *** （△ispB：：ddsA,△pykFA and △gapA：：gapC）,harboring the two plasmids encoding pck,dxs,idi and ubiCA genes under the control of PT5 on pQE30,ispA,ddsA from Gluconobacter suboxydans and gapC from Clostridium acetobutylicum under the control of PBAD on pBAD33,could produce CoQ10 up to 3.24 mg·g-1 dry cell mass simply by changing medium from M9YG to SOB with phosphate salt and initial culture pH from 7.0 to *** yield is unprecedented and 1.33 times of the highest production so far in ***.

关键词： coenzyme Q10 Escherichia coli gene replacement NADPH availability precursor balance

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Tomographic particle image velocimetry measurement on three-dimensional swirling flow in dual-stage counter-rotating swirler

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Chinese Journal of Aeronautics 2024年第3期37卷 7-20页

作者： Yuyang ZHOU Ling DONG mingtao SHANG Jibao LI Akira RINOSHIKA Key Laboratory of Fluid Mechanics of Ministry of Education Beihang UniversityBeijing 100191China School of Mechanical and Automotive Engineering Shanghai University of Engineering ScienceShanghai 201620China AECC Commercial Aircraft Engine Co. Ltd.Shanghai 200241China Department of Mechanical Systems Engineering Yamagata UniversityYamagata 992-8510Japan

Three-Dimensional(3D)swirling flow structures,generated by a counter-rotating dualstage swirler in a confined chamber with a confinement ratio of 1.53,were experimentally investigated at Re=2.3×10^(5)using Tomographic Particle Image Velocimetry(Tomo-PIV)and planar Particle Image Velocimetry(PIV).Based on the analysis of the 3D time-averaged swirling flow structures and 3D Proper Orthogonal Decomposition(POD)of the Tomo-PIV data,typical coherent flow structures,including the Corner Recirculation Zone(CRZ),Central Recirculation Zone(CTRZ),and Lip Recirculation Zone(LRZ),were *** counter-rotating dual-stage swirler with a Venturi flare generates the independence process of vortex breakdown from the main stage and pilot stage,leading to the formation of an LRZ and a smaller CTRZ near the nozzle *** confinement squeezes the CRZ to the corner and causes a reverse rotation flow to limit the shape of the CTRZ.A large-scale flow structure caused by the main stage features an explosive breakup,flapping,and Precessing Vortex Core(PVC).The explosive breakup mode dominates the swirling flow structures owing to the expansion and construction of the main jet,whereas the flapping mode is related to the wake *** limits the expansion of PVC and causes it to contract after the impacting area.

关键词： Coherent structures Dual-stage counter-rotating swirler cup Precessing vortex core Proper orthogonal decomposition Swirling flow Tomographic particle image velocimetry

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