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Carbon Catabolite Repressor UvCreA is Required for Development and Pathogenicity in Ustilaginoidea virens

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Rice science 2024年第2期31卷 203-214,I0029-I0031页

作者： XIE Shuwei shi huanbin WEN Hui LIU Zhiquan QIU Jiehua JIANG Nan KOU Yanjun State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 311400China

The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritionalconditions. Although significant progress has been made in understanding carbon catabolite repression infungi such as Aspergillus nidulans and Magnaporthe oryzae, its role in U. virens remains unclear. Toaddress this knowledge gap, we identified UvCreA, a pivotal component of carbon catabolite repression,in U. virens. Our investigation revealed that UvCreA localized to the nucleus. Deletion of UvCreA resultedin decreased growth and pathogenicity in U. virens. Through RNA-seq analysis, it was found that theknockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes. Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genesassociated with carbon metabolism in U. virens. In summary, our findings indicated that UvCreA isimportant in fungal development, virulence, and the utilization of carbon sources through transcriptionalregulation, thus making it a critical element of carbon catabolite repression.

关键词： Ustilaginoidea virens virulence carbon catabolite repression Oryza sativa rice false smut

Two H3K36 methyltransferases differentially associate with transcriptional activity and enrichment of facultative heterochromatin in rice blast fungus

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aBIOTECH 2024年第1期5卷 1-16页

作者： Mengting Xu Ziyue Sun huanbin shi Jiangnan Yue Xiaohui Xiong Zhongling Wu Yanjun Kou Zeng Tao Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China State Key Lab of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310021China

Di-and tri-methylation of lysine 36 on histone H3(H3K36me2/3)is catalysed by histone methyltransferase Set2,which plays an essential role in transcriptional *** there is a single H3K36 methyltransferase in yeast and higher eukaryotes,two H3K36 methyltransferases,Ash1 and Set2,were present in many filamentous ***,their roles in H3K36 methylation and transcriptional regulation remained *** with methods of RNA-seq and ChIP-seq,we revealed that both Ash1 and Set2 are redundantly required for the full H3K36me2/3 activity in Magnaporthe oryzae,which causes the devastating worldwide rice blast ***1 and Set2 distinguish genomic H3K36me2/3-marked regions and are differentially associated with repressed and activated transcription,***,Ash1-catalysed H3K36me2 was co-localized with H3K27me3 at the chromatin,and Ash1 was required for the enrichment and transcriptional silencing of H3K27me3-occupied *** the different roles of Ash1 and Set2,in H3K36me2/3 enrichment and transcriptional regulation on the stress-responsive genes,they differentially respond to various stresses in ***,we reveal a novel mechanism by which two H3K36 methyltransferases catalyze H3K36me2/3 that differentially associate with transcriptional activities and contribute to enrichment of facultative heterochromatin in eukaryotes.

关键词： Ash1 Facultative heterochromatin H3K36me2/3 Magnaporthe oryzae Set2 Transcriptional regulation

Putative Phosphatase UvPsr1 Is Required for Mycelial Growth, Conidiation, Stress Response and Pathogenicity in Ustilaginonidea virens

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Rice science 2020年第6期27卷 529-536页

作者： Xiong Meng Meng Shuai Qiu Jiehua shi huanbin Shen Xiangling Kou Yanjun Key Laboratory of Three Gorges Regional Plant Genetics&Germplasm Enhancement/Biotechnology Research Center China Three Gorges UniversityYichang 443000China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 310006China

Ustilaginoidea virens is the causal agent of rice false smut,which can be a highly destructive disease of *** plasma membrane phosphatase Psr1 proteins,which act as a regulator of the salinity stress response in yeast,are widely distributed across fungi,but their functional characterization is *** this study,we characterized the functions of Psr1 protein,UvPsr1,in *** of the AUvpsr1 and its complementation strain showed that UvPsr1 is required for normal mycelial growth,conidiation and tolerance to oxidative,osmotic and cell wall *** rice panicles were inoculated with the AUvpsr1 strains,no symptoms of false smut disease developed,showing that UvPSR1 also contributes to the pathogenicity of the *** deletion mutant of UvPSR1 also appeared to produce a smaller titer of toxic compounds able to inhibit elongation of the germinated *** conclusion,our results indicated that UvPsr1 is a new pathogenic factor of ***.

关键词： Psr1 rice false smut disease pathogenic factor clavicipitaceae Ustilaginoidea virens

UvWhi2 Is Required for Stress Response and Pathogenicity in Ustilaginoidea virens

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Rice science 2022年第1期29卷 47-54,I0018-I0023页

作者： MENG Shuai QIU Jiehua XIONG Meng LIU Zhiquan Jane Sadhna JAGERNATH LIN Fucheng shi huanbin KOU Yanjun State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310021China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of BiotechnologyZhejiang UniversityHangzhou 310058China

Some stress response-related genes have been identified in Ustilaginoidea virens,but it is not clear whether and how defects of stress responses affect the pathogenesis processes of *** this study,we identified a general stress response factor UvWHI2 as a homolog of Saccharomyces cerevisiae Whi2 in *** relative expression level of Uv Whi2 was significantly up-regulated during infection,suggesting that UvWHI2 may be involved in ***,knockout of Uv Whi2 showed decreased mycelial growth,increased conidiation in the potato sucrose medium and a defect in *** addition,the RNA-Seq and phenotypic analysis showed that UvWHI2 was involved in response to oxidative,hyperosmotic,cell wall stress and nutrient *** studies revealed that the defects of stress responses of the?Uvwhi2 mutant affected the formation of secondary spores on the nutrient limited surface and the rice surface,resulting in a significant reduction of pathogenicity of *** results suggest that UvWHI2 is necessary for fungal growth,stress responses and the formation of secondary spores in *** addition,the defects of stress responses can affect the formation of secondary spores on the rice surface,and then compromise the pathogenicity of ***.

关键词： Whi2 rice false smut Ustilaginoidea virens pathogenesis secondary spore

Warm temperature compromises JA-regulated basal resistance to enhance Magnaporthe oryzae infection in rice

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Molecular Plant 2022年第4期15卷 723-739页

作者： Jiehua Qiu Junhui Xie Ya Chen Zhenan Shen huanbin shi Naweed I.Naqvi Qian Qian Yan Liang Yanjun Kou State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China Temasek Life Sciences Laboratory and Department of Biological Sciences1 Research LinkNational University of SingaporeSingapore 117604Singapore College of Agriculture and Biotechnology Zhejiang UniversityHangzhou 310058China

Changes in global temperatures profoundly affect the occurrence of plant *** is well known that rice blast can easily become epidemic in relatively warm ***,the molecular mechanism remains *** this study,we show that enhanced blast development at a warm temperature(22C)compared with the normal growth temperature(28C)is rice *** transcriptome analysis revealed that jasmonic acid(JA)biosynthesis and signaling genes in rice could be effectively induced by Magnaporthe oryzae at 28C but not at 22*** analyses of the osaoc1 and osmyc2 mutants,OsCOI1 RNAi lines,and OsMYC2-OE plants further demonstrated that compromised *** JA biosynthesis and signaling lead to enhanced blast susceptibility at the warm *** with these results,we found that exogenous application of methyl jasmonate served as an effective strategy for improving blast resistance under the warm environmental ***,decreased activation of JA signaling resulted in the downregulated expression of some key basal resistance genes at 22C when compared with 28*** these affected genes,OsCEBiP(chitin elicitorbinding protein precursor)was found to be directly regulated by OsMYB22 and its interacting protein OsMYC2,a key component of JA signaling,and this contributed to temperature-modulated blast *** together,these results suggest that warm temperature compromises basal resistance in rice and enhances *** infection by reducing JA biosynthesis and signaling,providing potential new strategies for managing rice blast disease under warm climate conditions.

关键词： temperature-modulated resistance rice blast jasmonic acid CEBiP MYB22 plant immunity

Polycomb Repressive Complex 2-Mediated H3K27 Trimethylation Is Required for Pathogenicity in Magnaporthe oryzae

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Rice science 2022年第4期29卷 363-374页

作者： WU Zhongling QIU Jiehua shi huanbin LIN Chuyu YUE Jiangnan LIU Zhiquan XIE Wei Naweed INAQVI KOU Yanjun TAO Zeng Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China Temasek Life Sciences Laboratory and Department of Biological Sciences 1 Research LinkNational University of SingaporeSingapore 117604Singapore

Polycomb repressive complex 2(PRC2)contributes to catalyze the methylation of histone H3 at lysine 27 and plays vital roles in transcriptional silencing and growth development in various *** Magnaporthe oryzae,histone H3K27 is found to associate with altered transcription of in planta induced ***,it is still unknown whether and how H3K27me3 modification is involved in pathogenicity to rice and stress *** this study,we found that core subunits of PRC2,Kmt6-Suz12-Eed,were required for fungal pathogenicity to rice in ***6-Suz12-Eed localized in the nuclei and was necessary for the establishment of H3K27me3 *** ChIP-seq analysis,9.0%of genome regions enriched with H3K27me3 occupancy,which corresponded to 1033 genes in ***,deletion of Kmt6,Suz12 or Eed altered genome-wide transcriptional expression,while the de-repression genes in theΔkmt6 strain were highly associated with H3K27me3 ***,plenty of genes which encode effectors and secreted enzymes,secondary metabolite synthesis genes,and cell wall stress-responsive genes were directly occupied with H3K27me3 modification and de-repression in theΔkmt6 *** results elaborately explained how PRC2 was required for pathogenicity,which is closely related to effector modulated host immunity and host environment adaption.

关键词： rice blast H3K27me3 transcriptional regulation pathogenicity Polycomb repressive complex 2

Selective Degradation of Mitochondria by Mitophagy in Pathogenic Fungi

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American Journal of Molecular Biology 2021年第1期11卷 15-27页

作者： Jane Sadhna Jagernath Shuai Meng Jiehua Qiu huanbin shi Yanjun Kou State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou China

Selective mitochondrial autophagy or mitophagy is an evolutionarily conserved cellular process that selectively degrades superfluous, damaged, and dysfunctional mitochondria. This process is believed to be a mitochondrial quality control system crucial for intracellular homeostasis. Recently, researchers developed a range of methods to induce mitophagy and a variety of assays to monitor this process. With these new methods, the research on mitophagy has been developed rapidly. In particular, some key receptors and regulatory factors in fungi have been identified, which provides a basis for further understanding of the mechanism of this process. Although it has been studied extensively in the model yeast Saccharomyces cerevisiae, mitophagy in pathogenic fungi remains poorly understood. However recent studies have shown that mitophagy is involved in the regulation of pathogenicity of pathogenic fungi, which greatly increases the importance of mitophagy. Therefore, it is necessary to review the current research on mitophagy in order to provide an accurate understanding of mitophagy and promote mitophagy research in the pathogenic fungi.

关键词： Mitochondria Mitophagy Yeast Pathogenic Fungi

The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast

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Plant Communications 2023年第5期4卷 309-323页

作者： Zhiquan Liu Jiehua Qiu Zhenan Shen Congcong Wang Nan Jiang huanbin shi Yanjun Kou State Key Laboratory of Rice Biology China National Rice Research InstituteHangzhou 311400China

Rice blast,caused by Magnaporthe oryzae,is one of the most devastating diseases of *** infection,*** secretes effectors to facilitate blast *** these effectors,the avirulence factor AvrPi9 is recognized by Pi9,a broad-spectrum blast resistance protein that triggers Pi9-mediated resistance in ***,little is known about the interaction between AvrPi9 and Pi9 and how AvrPi9 exerts virulence to promote *** this study,we found that ectopic expression of AvrPi9 in the Pi9-lacking cultivar TP309 suppressed basal resistance against ***,we identified an AvrPi9-interacting protein in rice,which we named OsRGLG5,encoding a functional RING-type E3 ubiquitin *** infection,AvrPi9 was ubiquitinated and degraded by ***,AvrPi9 affected the stability of *** assays revealed that OsRGLG5 is a positive regulator of basal resistance against ***,but it is not essential for Pi9-mediated blast resistance in *** conclusion,our results revealed that OsRGLG5 is targeted by the *** effector AvrPi9 and positively regulates basal resistance against rice blast.

关键词： Oryza sativa disease resistance avirulence factor rice-Magnaporthe oryzae interaction plant defense

南方稻区稻瘟病菌株中无毒基因AvrPi9的变异分析

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植物病理学报 2022年第4期52卷 565-572页

作者：沈浙南时焕斌邱结华中国农业科学院中国水稻研究所/水稻生物学国家重点实验室杭州311400

本研究通过PCR(Polymerase Chain Reaction)和测序技术以及稻瘟病菌致病力检测手段,分析了2020年来自安徽、重庆、福建、广西、湖南、江苏、江西、云南以及浙江等省(直辖市、自治区)的300个稻瘟病分离菌株的无毒基因AvrPi9的分布和变异... 详细信息

本研究通过PCR(Polymerase Chain Reaction)和测序技术以及稻瘟病菌致病力检测手段,分析了2020年来自安徽、重庆、福建、广西、湖南、江苏、江西、云南以及浙江等省(直辖市、自治区)的300个稻瘟病分离菌株的无毒基因AvrPi9的分布和变异情况。结果表明,298份稻瘟病菌分离菌株的AvrPi9位点能被有效扩增,扩增产物经测序分析后显示,其中8株供试菌株的AvrPi9基因位点的外显子区域253位发生单碱基变异(C碱基替换成T碱基,AvrPi9^(C253T)),导致转录提前终止。稻瘟病菌致病力分析表明,AvrPi9^(C253T)菌株对带有Pi9基因的植株TP309-Pi9产生了致病性,说明该类菌株的AvrPi9位点变异后不能被Pi9识别。上述结果提示培育和种植携带有抗稻瘟病基因Pi9的品种可以较大程度上对南方稻区稻瘟病起到防控作用。这对保障水稻生产安全具有重要意义。

关键词：稻瘟病无毒基因 AvrPi9 毒力变异