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Effects of hepatitis B virus infection on human sperm chromosomes

World Journal of Gastroenterology 2003年第4期9卷 736-740页

作者： Jian-Minhuang Tian-Huahuang huan-yingqiu Xiao-WuFang Tian-GangZhuang Hong-XiLiu Yong-HuaWang, Li-ZhiDeng Jie-WenQiu DepartmentofCellBiologyandMedicalGenetics ShantouUniversityMedicalCollegeShantou515031GuangdongProvinceChina ShantouUniversityHospital Shantou515063GuangdongProvinceChina DepartmentofInternalMedicine SecondAffiliatedHospitalShantouUniversityMedicalCollegeShantou515041GuangdongProvinceChina

AIM: To evaluate the level of sperm chromosome aberrations in male patients with hepatitis B, and to directly detect whether there are HBV DNA integrations in sperm chromosomes of hepatitis B ***: Sperm chromosomes of 14 tested subjects (5healthy controls, 9 patients with HBV infection, including 1with acute hepatitis B, 2 with chronic active hepatitis B, 4with chronic persistent hepatitis B, 2 chronic HBsAg carriers with no clinical symptoms) were prepared using interspecific in vitro fertilization between zona-free golden hamster ova and human spermatozoa, and the frequencies of aberration spermatozoa were compared between subjects of HBV infection and controls. Fluorescence in situ hybridization (FISH) to sperm chromosome spreads was carried out with biotin-labeled full length HBV DNA probe to detect the specific HBV DNA sequences in the sperm ***: The total frequency of sperm chromosome aberrations in HBV infection group (14.8%, 33/223) was significantly higher than that in the control group (4.3%,5/116). Moreover, the sperm chromosomes in HBV infection patients commonly presented stickiness, clumping, failure to staining, etc, which would affect the analysis of sperm chromosomes. Specific fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one patient with chronic persistent hepatitis. In 9 (9/42) sperm chromosome complements containing fluorescent signal spots, one presented 5 obvious FISH spots, others presented 2 to 4signals. There was significant difference of fluorescence intensity among the signal spots. The distribution of signal sites among chromosomes was ***: HBV infection can bring about mutagenic effects on sperm chromosomes. Integrations of viral DNA into sperm chromosomes which are multisites and nonspecific, can further increase the instability of sperm chromosomes. This study suggested that HBV infection can create extensively hereditary effects by alteration genetic constituent and/o

关键词： Adult Chromosomes, Human DNA, Viral Hepatitis B Antigens Hepatitis B Surface Antigens Hepatitis B, Chronic Humans In Situ Hybridization, Fluorescence Karyotyping Male Reference Values Research Support, Non-U.S. Gov't Semen Spermatozoa

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Two paths, one goal: mechanism of calcium homeostasis to balance plant growth and immunity

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Science China Life Sciences 2025年第1期68卷 306-308页

作者： Aziz Ul Ikram Yanping Jing huan Chen Jian Chen International Genome Center Jiangsu University Joint Center for Single Cell Biology School of Agriculture and BiologyShanghai Jiao Tong University

Calcium(Ca2+) is an essential nutrient and a cellular signal in plants. The level of free Ca2+in the apoplast is ～10,000-fold higher than that in the cytoplasm at the resting state. This large Ca2+level gradient across the membrane between the interior and exterior can cause Ca2+overload and toxicity. Plants have evolved mechanisms to export Ca2+out of the cytoplasm or store it in intracellular organelles, such as endoplasmic reticulum（ER）, mitochondria, and vacuole.

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Dual modulation of homogeneous nanomaterialization and electrochemical activation enhancing zinc ion storage

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Science China Chemistry 2025年第1期68卷 385-393页

作者： Jianwei Wang huan Wang Kangning Wang Wenhui Wang Wenlin Zhang Yanzhong Zhen School of Chemistry & Chemical Engineering Yan'an University

Vanadium-based electrode materials are widely investigated,but the low specific capacity and slow electrochemical kinetics in aqueous zinc-ion batteries still limit their commercial ***,the VS2/Ca V4O9material with the morphology of nanoflower was synthesized by a one-step hydrothermal *** to the blocky structure of pure VS2material,the VS2/Ca V4O9material is composed of thinner homogeneous *** open structures could provide abundant electrochemical active sites and ion transport channels,and then promote the electrochemical reaction *** addition,they can also buffer the bulk strain during the reaction *** improve the utilization of vanadium elements,an in-situ electrochemical activation strategy is used to explore the storage performance of the VS2/Ca V4O9material,the different activation voltage range of 0.4–1.6 and 0.4–1.4 V are selected,*** with the longer activation plateau of activated-VS2,the VS2/Ca V4O9cathode could quickly reach the activation state in the range of 1.4–1.6 V and cause the release of additional Zn storage sites *** VS2/Ca V4O9cathode delivers a higher power density of 37,000 W kg-1and a significant energy density of 423 Wh *** the high current density of 15 A g-1,the VS2/Ca V4O9cathode still has a discharge capacity of 183.9m Ah g-1after 5,000 cycles,and the capacity decay rate per cycle is only 0.0042%.Continuous cyclic voltammetry （CV） curves,electrochemical impedance spectroscopy （EIS） measurements,density functional theory （DFT） calculation and galvanostatic intermittent titration technique （GITT） measurements demonstrate that the VS2/Ca V4O9cathode has a faster ion diffusion/charge transfer ***,the assembled flexible device has an excellent mechanical stability.

关键词： dual modulation homogeneous nanomaterialization electrochemical activation zinc ion storage

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Study on the hydrogen absorption properties of a YGdTbDyHo rare-earth high-entropy alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 127-135页

作者： Tongyue Li Ziliang Xie Wenjiao Zhou huan Tong Dawen Yang Anjia Zhang Yuan Wu Xiping Song State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing 100083China Whole Win(Beijing)Materials Sci.&Tech.Co. Ltd.Beijing 100083China

This study investigated the microstructure and hydrogen absorption properties of a rare-earth high-entropy alloy(HEA),*** indicated that the YGdTbDyHo alloy had a microstructure of equiaxed grains,with the alloy elements distributed *** hydrogen absorption,the phase structure of the HEA changed from a solid solution with an hexagonal-close-packed(HCP)structure to a high-entropy hydride with an faced-centered-cubic(FCC)structure without any secondary phase *** alloy demonstrated a maximum hydrogen storage capacity of 2.33 H/M(hydrogen atom/metal atom)at 723 K,with an enthalpy change(ΔH)of-141.09 kJ·mol^(-1)and an entropy change(ΔS)of-119.14 J·mol^(-1)·K^(-1).The kinetic mechanism of hydrogen absorption was hydride nucleation and growth,with an apparent activation energy(E_(a))of 20.90 kJ·mol^(-1).Without any activation,the YGdTbDyHo alloy could absorb hydrogen quickly(180 s at 923 K)with nearly no incubation period *** reason for the obtained value of 2.33 H/M was that the hydrogen atoms occupied both tetrahedral and octahedral *** results demonstrate the potential application of HEAs as a high-capacity hydrogen storage material with a large H/M ratio,which can be used in the deuterium storage field.

关键词： rare-earth high-entropy alloy hydrogen absorption capacity pressure–composition–temperature curves kinetics

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Deep learning identification of novel autophagic protein-protein interactions and experimental validation of Beclin 2-Ubiquilin 1 axis in triple-negative breast cancer

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Oncology Research 2025年第1期33卷 67-81页

作者： XIANG LI WENKE JIN LIFENG WU huan WANG XIN XIE WEI huanG BO LIU State Key Laboratory of Southwestern Chinese Medicine Resources School of Pharmacy and College of Medical TechnologyChengdu University of Traditional Chinese MedicineChengdu611137China Department of Biotherapy Cancer Center and State Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu610041China

Background:Triple-negative breast cancer(TNBC),characterized by its lack of traditional hormone receptors and HER2,presents a significant challenge in oncology due to its poor response to conventional *** is an important process for maintaining cellular homeostasis,and there are currently autophagy biomarkers that play an effective role in the clinical treatment of *** contrast to targeting protein activity,intervention with proteinprotein interaction(PPI)can avoid unrelated crosstalk and regulate the autophagy process with minimal interference ***:Here,we employed Naive Bayes,Decision Tree,and k-Nearest Neighbors to elucidate the complex PPI network associated with autophagy in TNBC,aiming to uncover novel therapeutic ***,the candidate proteins interacting with Beclin 2 were initially screened in MDA-MB-231 cells using Beclin 2 as bait protein by immunoprecipitation-mass spectrometry assay,and the interaction relationship was verified by molecular docking and CO-IP experiments after *** formation,cellular immunofluorescence,cell scratch and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)tests were used to predict the clinical therapeutic effects of manipulating candidate ***:By developing three PPI classification models and analyzing over 13,000 datasets,we identified 3733 previously unknown autophagy-related *** network analysis revealed the central role of Beclin 2 in autophagy regulation,uncovering its interactions with 39 newly identified ***,the CO-IP studies identified the substantial interaction between Beclin 2 and Ubiquilin 1,which was anticipated by our model and discovered in immunoprecipitation-mass spectrometry assay ***,in vitro investigations showed that overexpressing Beclin 2 increased Ubiquilin 1,promoted autophagy-dependent cell death,and inhibited proliferation and metastasis in MDA-MB-231 ***:This study not

关键词： Triple-negative breast cancer(TNBC) Autophagy Protein-protein interactions(PPI) Artificial intelligence(AI) Beclin 2 Ubiquilin 1

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Research progress in metal-organic frameworks and their derivatives in electrochemistry

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Science China Chemistry 2025年

作者： Lingling Chen Yuxin Liu Yichun Su Wenchen Zhu Guangyu Du huan Pang School of Chemistry and Chemical Engineering Yangzhou University Department of Applied Physics The Hong Kong Polytechnic University Interdisciplinary Materials Research Center Institute for Advanced Study Chengdu University State Key Laboratory of Coordination Chemistry Nanjing University

Metal-organic frameworks (MOFs) are distinguished by their unique porosity and meticulously ordered framework structures,setting them apart from conventional complexes. These materials are defined by their highly organized crystalline and pore structures, extensive surface areas, robust porosity, and superior adsorption capabilities. Owing to their exceptional structural and functional attributes, MOFs and their derivatives are leveraged across a broad spectrum of applications, demonstrating significant potential across diverse sectors. In recent times, the scientific community has increasingly focused on MOFs, underscoring their growing importance in research. Despite the promising array of applications for MOFs and their derivatives,challenges persist in practical deployments, and the pathway to research advancement remains daunting. This document delves into the synthesis and various applications of MOFs and their derivatives, particularly in the domains of energy storage, catalysis,sensing, and water treatment. It also highlights the ongoing research progress in the field of electrochemistry, including developments in lithium ion batteries (LIBs), lithium-sulfur batteries (LSBs), zinc-ion batteries (ZIBs), aqueous nickel-zinc batteries (NZBs), and supercapacitors. Finally, it provides an outlook on the future prospects and potential hurdles facing the development of MOF materials.

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Modeling and control of a rigid-flexible coupling robot for narrow space manipulations

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Science China Technological Sciences 2025年

作者： Kuangda CHEN huan ZHAO Jiexin ZHANG Mei FENG Yiwei WANG Jinliang WANG Han DING State Key Laboratory of Intelligent Manufacturing Equipment and Technology Huazhong University of Science and Technology Key Laboratory of CNC Equipment Reliability Ministry of Education Jilin University School of Medical Equipment Science and Engineering Huazhong University of Science and Technology

Narrow space manipulations are crucial for equipment maintenance, as they eliminate the need for disassembly, thus reducing costs and increasing efficiency. Cable-driven flexible robots possess exceptional capabilities in maneuvering through narrow spaces, thanks to their interaction compliance and distal dexterity. However, their inherent low stiffness causes a decline in the location accuracy of the robot and could even lead to manipulation failure. To address this challenge, this paper proposes a novel type of rigid-flexible coupling robot, which increases the stiffness of the robot while ensuring the reachability within a narrow space. To achieve high-accuracy location in a narrow space, kinematic and control methods are proposed. Specifically,the robot forward kinematics are uniformly represented, and the closed-form inverse kinematics solution is derived. Furthermore, in cases where the robot?s structure fails to align with Pieper?s solution, a two-stage search strategy is utilized to solve the inverse kinematics, resulting in a high success rate of 99.1%. Based on the proposed kinematics, a rigid-dominated control method is then proposed to facilitate localization and pose alignment of target objects in narrow spaces. The practical comparative experiments show that the proposed robot has the capability of high accuracy control in narrow spaces.

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Insufficient MIRO1 contributes to declined oocyte quality during reproductive aging

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Science China Life Sciences 2025年

作者： Zhen-Nan Pan Hao-Lin Zhang Kun-huan Zhang Jia-Qian Ju Jing-Cai Liu Shao-Chen Sun College of Animal Science and Technology Nanjing Agricultural University Reproductive Medicine Centre The Affiliated Yantai Yuhuangding Hospital of Qingdao University

Mitochondrial Rho-GTPase 1 (MIRO1) is an outer mitochondrial membrane protein which regulates mitochondrial transport and mitophagy in mitosis. In present study, we reported the crucial roles of MIRO1 in mammalian oocyte meiosis and its potential relationship with aging. We found that MIRO1 expressed in mouse and porcine oocytes, and its expression decreased in aged mice. MIRO1 deficiency caused the failure of meiotic resumption and polar body extrusion in both mouse and porcine oocytes, which could be rescued by exogenous MIRO1supplementation. Mass spectrometry data indicated that MIRO1 associated with several cytoskeleton and cell cycle-related proteins, and MIRO1 regulated motor protein Dynein for microtubule-organizing centers (MTOCs) dynamics at germinal vesicle (GV) stage, which determined meiotic resumption. Furthermore, we found that MIRO1 regulated Aurora A and kinesin family member 11 (KIF11) for meiotic spindle assembly in oocytes. Besides, MIRO1 associated with several mitochondria-related proteins dynamic-related protein 1 (DRP1),Parkin and lysosomal-associated membrane protein 2 (LAMP2) for mitochondrial dynamics and mitophagy during oocyte meiosis. Taken together, our results suggested that MIRO1 played pivotal roles in meiotic resumption, spindle assembly and mitochondrial function in mouse and porcine oocytes, and its insufficiency might contribute to the oocyte maturation defects during aging.

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几种海洋硬骨鱼类快、慢肌的化学组分差异分析

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渔业科学进展 2025年第1期46卷 59-70页

作者：王焕柳淑芳海水养殖生物育种与可持续产出全国重点实验室中国水产科学研究院黄海水产研究所山东青岛266071 青岛海洋科技中心海洋渔业科学与食物产出过程功能实验室山东青岛266237

硬骨鱼类的骨骼肌根据收缩特征主要分为快肌和慢肌,分别支撑爆发性和持续性游泳。为认识这2种骨骼肌的化学组分特征,解析快、慢肌功能差异的物质基础,本研究整合3类不同游泳习性鱼类:黄带拟鲹(Pseudocaranx dentex)、梭鱼(Liza haematoc... 详细信息

硬骨鱼类的骨骼肌根据收缩特征主要分为快肌和慢肌,分别支撑爆发性和持续性游泳。为认识这2种骨骼肌的化学组分特征,解析快、慢肌功能差异的物质基础,本研究整合3类不同游泳习性鱼类:黄带拟鲹(Pseudocaranx dentex)、梭鱼(Liza haematocheila)和金枪鱼类,通过自测结合文献资料,比较了快、慢肌在蛋白质、氨基酸、脂肪、脂肪酸及矿物元素等化学组分特征方面的差异。结果显示,快肌的粗蛋白及12种氨基酸含量更为丰富,其中尤以组氨酸含量差异最大(快肌为慢肌的1.22~3.83倍);快、慢肌的主要氨基酸组成相似,必需氨基酸含量均占氨基酸总量的40%左右,谷氨酸和天冬氨酸是含量最丰富的2种氨基酸类型,赖氨酸和亮氨酸是含量最高的2种必需氨基酸;慢肌的粗脂肪及每种脂肪酸的含量均显著高于快肌;在脂肪酸组成方面,慢肌中饱和脂肪酸(saturated fatty acid,SFA)的比例较快肌高,而快肌中的多不饱和脂肪酸(polyunsaturated fatty acid,PUFA)比例则比慢肌高;慢肌含有更为丰富的微量元素铁(Fe)和锌(Zn)。结果表明,海洋硬骨鱼类的快肌和慢肌在蛋白质、氨基酸、脂肪、脂肪酸及矿物元素组成方面存在较大的差异,这些差异为其分别支撑爆发性游泳运动和持续性游泳运动提供了一定的物质基础。

关键词：海洋硬骨鱼类快肌慢肌化学成分特征

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The shifts of precipitation phases and their impacts

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Science China Earth Sciences 2025年

作者： Xuemei LI Tao CHE Yuanlong TANG huan'e DUAN Guigang WANG Xu ZHANG Chuanming YANG Jun WU Yue ZHANG Lanhai LI Faculty of Geomatics Lanzhou Jiaotong University National-Local Joint Engineering Research Center of Technologies and Applications for National Geographic State Monitoring Key Laboratory of Science and Technology in Surveying & Mapping Gansu Province Key Laboratory of Cryospheric Science and Frozen Soil Engineering Heihe Remote Sensing Experimental Research StationNorthwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Xinjiang Key Laboratory of Water Cycle and Utilization in Arid Zone Xinjiang Institute of Ecology and Geography Chinese academy of Sciences Tianshan Snow Cover and Avalanche Observation and Research Station of Xinjiang

Precipitation comes in various phases, including rainfall, snowfall, sleet, and hail. Shifts of precipitation phases, as well as changes in precipitation amount, intensity, and frequency, have significant impacts on regional climate, hydrology,ecology, and the energy balance of the land-atmosphere system. Over the past century, certain progress has been achieved in aspects such as the observation, discrimination, transformation, and impact of precipitation phases. Mainly including:since the1980s, studies on the observation, formation mechanism, and prediction of precipitation phases have gradually received greater attention and reached a certain scale. The estimation of different precipitation phases using new detection theories and methods has become a research focus. Avariety of discrimination methods or schemes, such as the potential thickness threshold method of the air layer, the temperature threshold method of the characteristic layer, and the near-surface air temperature threshold method,have emerged one after another. Meanwhile, comparative studies on the discrimination accuracy and applicability assessment of multiple methods or schemes have also been carried out simultaneously. In recent years, the shift of precipitation from solid to liquid (SPSL) in the mid-to-high latitudes of the Northern Hemisphere has become more pronounced due to global warming and human activities. It leads to an increase in rain-on-snow (ROS) events and avalanche disasters, affecting the speed, intensity, and duration of spring snow-melting, accelerating sea ice and glacier melting, releasing carbon from permafrost, altering soil moisture, productivity, and phenological characteristics of ecosystems, and thereby affecting their structures, processes, qualities, and service functions. Although some progress has been made in the study of precipitation phases, there remains considerable research potential in terms of completeness of basic data, reliability of discrimination schemes,

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