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Nia1 and Nia2 are Involved in Exogenous Salicylic Acid-induced Nitric Oxide Generation and Stomatal Closure in Arabidopsis

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Journal of Integrative Plant Biology 2010年第3期52卷 298-307页

作者： Fushun Hao Shiling Zhao huan Dong huan zhang Lirong Sun Chen Miao Henan Key Laboratory of Plant Stress Biology College of Life Science Henan University Kaifeng 475004 China

Phytohormone salicylic acid （SA） plays important roles in plant responses to environmental stress. However, knowledge about the molecular mechanisms for SA affecting the stomatal movements is limited. In this paper, we demonstrated that exogenous SA significantly induced stomatal closure and nitric oxide （NO） generation in Arabidopsis guard cells based on genetic and physiological data. These effects were significantly inhibited by the NO scavenger c-PTIO, NO synthase （NOS） inhibitor L-NAME or nitrate reductase suppressor tungstate respectively, implying that NOS and nitrate reductase （NR） participate in SA-evoked stomatal closing. Furthermore, the effects of SA promotion of stomatal closure and NO synthesis are significantly suppressed in NR single mutants of nial, nia2 or double mutant niallnia2, compared with the wild type plants. This suggests that both Nial and Nia2 are involved in SA-stimulated stomatal closure. In addition, pharmacological experiments showed that protein kinases, cGMP and cADPR are involved in SA-mediated NO accumulation and stomatal closure induced by SA in Arabidopsis.

关键词： SALICYLIC acid ARABIDOPSIS PLANT hormones NITRIC oxide TUNGSTATES

Dewetting Kinetics of Thin Polymer Films with Different Architectures:Effect of Polymer Adsorption

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Chinese Journal of Polymer Science 2018年第8期36卷 984-990页

作者： Li-Na Wang huan-huan zhang Lin Xu Bin-Yuan Liu Tong-Fei Shi Shi-Chun Jiang Li-Jia An School of Materials Science and Engineering Tianjin University State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied ChemistryChinese Academy of Sciences Laboratory of Surface Physics and Chemistry Guizhou Education University Department of Polymer Science and Engineering Hebei University of Technology

We have investigated the influence of the adsorption process on the dewetting behavior of the linear polystyrene film （LPS）, the 3-arm star polystyrene film （3SPS） and the ring polystyrene film （RPS） on the silanized Si substrate. Results show that the adsorption process greatly influences the dewetting behavior of the thin polymer films. On the silanized Si substrate, the 3SPS chains exhibit stronger adsorption compared with the LPS chains and RPS chains; as a result, the wetting layer forms more easily. For LPS films, with the decrease of annealing temperature, the kinetics of polymer film changes from exponential behavior to slip dewetting. As a comparison, the stability of 3SPS and RPS films switches from slip dewetting to unusual dewetting kinetic behavior. The adsorbed nanodroplets on the solid substrate play an important role in the dewetting kinetics by reducing the driving force of dewetting and increase the resistant force of dewetting. Additionally, Brownian dynamics （BD） simulation shows that the absolute values of adsorption energy （ε） gradually increase from linear polymer （-0.3896） to ring polymer （-0.4033） and to star polymer （-0.4264）, which is consistent with the results of our adsorption experiments.

关键词： Thin polymer film Star polystyrene Ring polystyrene Dewetting Adsorption

Analysis of SSRs Information in Capsicum spp. from EST Database

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Agricultural Sciences in China 2011年第10期10卷 1532-1536页

作者： huanG huan-huan zhang Zhong-hua zhang Zheng-hai MAO Sheng-li WANG Li-hao zhangBao-xi Key Laboratory of Vegetable Genetics and Physiology Ministry of Agriculture~Institute of Vegetables and Flowers Chinese Academy ofAgricultural Sciences Beijing 100081 P.R. China

SSR markers are useful in pepper linkage mapping and gene location. 446 SSR markers have been reported, but they are insufficient. It is costly to develop SSR markers from DNA library, whereas it seems much easy to find in EST sequences in the GenBank of pepper through internet. In this study, attempts have been made to develop SSR markers in the EST sequences by using bioinformatics. EST sequences were trimmed by ‘***’ software, while 116 915 EST sequences were obtained without poly ‘A’ or poly ‘T’, ranged between 100 and 700 bp. Using ‘e-PCR’ and ‘***’ softwares, SSR sequences were identified. 2 508 microsatellite loci （larger than 20 repeats） were established and 755 SSR primers were designed using SSR finder software and Primer 3 software. There were 498 （0.43%） mono-, 1 026 （0.89%） di-, 518 （0.45%） tri-, 245 （0.21%） tetra-, 114 （0.10%） penta-, and 107 （0.09%） hexa-nucleotide SSRs. The estimated frequency of SSRs was approximately 1/25.12 kb. According to the distribution of SSRs in pepper, the mean length of pepper SSRs was 22.68 bp and the adenine rich repeats such as A/T, AG, AT, AAG, AAAT, and AAAC were predominant in each type of SSRs （mono-, di-, tri-, tetra-, penta-, and hexa-）, whereas the C/G, CG, CCG repeats were less abundant. 210 primers were tested in 8 pepper cultivars and the PCR result revealed the existence of polymorphism among 127 （60.48%） SSR primers within 8 pepper cultivars. It is confirmed that pepper EST database could be efficiently exploited for availability of SSR markers.

关键词： pepper expressed sequence tags microsatellite bioinformatics e-PCR

Functionalization of nanodiamond with epoxy monomer

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Chinese Chemical Letters 2011年第4期22卷 485-488页

作者： huan huan zhang Ya Ting Liu Rong Wang Xiao Yan Yu Xiong Wei Qu Qing Xin zhang Institute of Polymer Science and Engineering School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300130China

A novel nanodiamond-epoxy derivative（ND-EP） was synthesized by grafting epoxy monomers onto the surface of nanodiamond（ND）,and characterized by FTIR and *** ratio of grafted epoxy groups was determined to be 32.5 wt% by *** developed methodology provides an efficient method for the functionalization of nanodiamond material,which enables a variety of advanced engineering and biomedical applications of ND.

关键词： Nanodiamond Epoxy Surface modification

Mutations of beta-amyloid precursor protein alter the consequence of Alzheimer's disease pathogenesis

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Neural Regeneration Research 2019年第4期14卷 658-665页

作者： Nuo-Min Li Ke-Fu Liu Yun-Jie Qiu huan-huan zhang Hiroshi Nakanishi Hong Qing School of Life Science Beijing Institute of Technology Department of Aging Science and Pharmacology Faculty of Dental Science Kyushu University

Alzheimer's disease is pathologically defined by accumulation of extracellular amyloid-β(Aβ). Approximately 25 mutations in β-amyloid precursor protein(APP) are pathogenic and cause autosomal dominant Alzheimer's disease. To date, the mechanism underlying the effect of APP mutation on Aβ generation is unclear. Therefore, investigating the mechanism of APP mutation on Alzheimer's disease may help understanding of disease pathogenesis. Thus, APP mutations(A673T, A673 V, E682 K, E693 G, and E693Q) were transiently co-transfected into human embryonic kidney cells. Western blot assay was used to detect expression levels of APP, beta-secretase 1, and presenilin 1 in cells. Enzyme-linked immunosorbent assay was performed to determine Aβ_(1–40) and Aβ_(1–42) levels. Liquid chromatography-tandem mass chromatography was used to examine VVIAT, FLF, ITL, VIV, IAT, VIT, TVI, and VVIA peptide levels. Immunofluorescence staining was performed to measure APP and early endosome antigen 1 immunoreactivity. Our results show that the protective A673 T mutation decreases Aβ_(42)/Aβ_(40) rate by downregulating IAT and upregulating VVIA levels. Pathogenic A673 V, E682 K, and E693 Q mutations promote Aβ_(42)/Aβ_(40) rate by increasing levels of CTF99, Aβ_(42), Aβ_(40), and IAT, and decreasing VVIA levels. Pathogenic E693 G mutation shows no significant change in Aβ_(42)/Aβ_(40) ratio because of inhibition of γ-secretase activity. APP mutations can change location from the cell surface to early endosomes. Our findings confirm that certain APP mutations accelerate Aβ generation by affecting the long Aβ cleavage pathway and increasing Aβ_(42/40) rate, thereby resulting in Alzheimer's disease.

关键词： nerve regeneration Alzheimer’s disease β-amyloid precursor protein amyloidβ APP mutations liquid chromatography-tandem mass chromatography cellular localization long Aβ neural regeneration

Scalable synthesis of mesoporous FeS_(2) nanorods as highperformance anode materials for sodium-ion batteries

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Rare Metals 2022年第1期41卷 21-28页

作者： Hong-Yan Liang Zhi-Wen zhang Xiao-Bin Zhong Yao-Hui zhang Meng-Yao Tang Shu-Xian Li huan-huan zhang Peng-Fei Hu Jun-Fei Liang School of Energy and Power Engineering North University of ChinaTaiyuan 030051China School of Chemistry Beihang UniversityBeijing 100191China

Sodium-ion batteries(SIBs),as highly promising alternatives to lithium-ion batteries(LIBs),can be widely used in a variety of next-generation energy storage ***,the current commercial graphite anodes of LIBs could not intercalate sodium ions to appreciable extent,and the electrochemical irreversibility hinders further *** for a suitable anode material is a critical issue for the successful development of ***,we report a convenient,fast,and large-scale preparation method of mesoporous FeS_(2) *** specially designed one-dimensional mesoporous structure of FeS_(2) takes full advantage of ultra-high strain relaxation as well as fast Na^(+)transport rate arising from microstructural *** a result,the mesoporous FeS_(2) nanorods exhibited excellent sodium storage *** discharge capacity was retained at 711.1 mAh·g^(-1) after 450 cycles at a current density of 1000 mA·g^(-1).The special microstructure and superior performance of mesoporous FeS_(2) nanorods represent a critical step for transition metal sulfides electrode materials toward practical SIBs application.

关键词： FeS_(2) critical relaxation

Baculoviral inhibitor of apoptosis protein repeatcontaining protein 3 delays early Wallerian degeneration after sciatic nerve injury

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Neural Regeneration Research 2022年第4期17卷 845-853页

作者： Min Cai Jian Shao Bryant Yung Yi Wang Nan-Nan Gao Xi Xu huan-huan zhang Yu-Mei Feng Deng-Bing Yao Nantong University Medical School NantongJiangsu ProvinceChina School of Life Sciences Jiangsu Key Laboratory of NeuroregenerationCo-innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of Hand Surgery China-Japan Union Hospital of Jilin UniversityChangchunJilin ProvinceChina

Wallerian degeneration is a complex biological process that occurs after nerve injury,and involves nerve degeneration and *** cells play a crucial role in the cellular and molecular events of Wallerian degeneration of the peripheral nervous ***,Wallerian degeneration regulating nerve injury and repair remains largely unknown,especially the early *** have previously reported some key regulators of Wallerian degeneration after sciatic nerve *** inhibitor of apoptosis protein repeat-containing protein 3(BIRC3)is an important factor that regulates apoptosis-inhibiting *** this study,we established rat models of right sciatic nerve *** vitro Schwann cell models were also established and subjected to gene transfection to inhibit and overexpress *** data indicated that BIRC3 expression was significantly up-regulated after sciatic nerve *** BIRC3 upregulation and downregulation affected the migration,proliferation and apoptosis of Schwan cells and affected the expression of related factors through activating c-fos and ERK signal *** of BIRC3 delayed early Wallerian degeneration through inhibiting the apoptosis of Schwann cells after sciatic nerve *** findings suggest that BIRC3 plays an important role in peripheral nerve injury repair and *** study was approved by the Institutional Animal Care and Use Committee of Nantong University,China(approval No.2019-nsfc004)on March 1,2019.

关键词： apoptosis baculoviral inhibitor of apoptosis protein repeat-containing protein 3 nerve degeneration rat Schwann cell sciatic nerve injury signal pathway Wallerian degeneration

Interfacial active sites on Co-Co_(2)C@carbon heterostructure for enhanced catalytic hydrogen generation

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Rare Metals 2023年第6期42卷 1935-1945页

作者： huan-huan zhang Yang-Bin Ren Zhen-Luo Yuan Nai-Xin Kang Sehrish Mehdi Cong-Cong Xing Xian-Yun Liu Yan-Ping Fan Bao-Jun Li Bao-Zhong Liu College of Chemistry and Chemical Engineering Henan Polytechnic UniversityJiaozuo454003China ISM UMR CNRS No 5255Univ.BordeauxTalence Cedex33405France Department of Chemistry The Women University MultanKutchery CampusMultan66000Pakistan Department of Materials Science and Engineering Pennsylvania State UniversityUniversity ParkPA16802USA Research Center of Green Catalysis College of ChemistryZhengzhou UniversityZhengzhou450001China Henan Key Laboratory of Coal Green Conversion Henan Polytechnic UniversityJiaozuo454003China

Designing catalysts with capable dual-active sites to drive catalytic hydrogen generation is necessary for the future hydrogen ***,the interfacial active sites consisting of Co and Co-C on Co-Co_(2)C@carbon heterostructure are designed through annealing and highpressure *** operating temperature during the high-pressure carbonization under a CO-reducing environment is responsible for the construction and regulation of Co-Co_(2)C@C *** optimal catalyst has a high turnover frequency(TOF) of33.1 min^(-1) and low activation energy(E_a) of27.3 kJ-mol^(-1) during the hydrolysis of NH_(3)BH_(3).The catalytic stability of Co-Co_(2)C@C has no dramatic deterioration even after 5 cyclic *** interfacial active sites and the carbon on the catalyst surface enhance hydrogen generation kinetics and catalytic *** construction of interfacial active sites in Co-Co_(2)C@C prompts the dissociation of reactants(NH_(3)BH_(3) and H_(2)O molecules),leading to an enhanced catalytic hydrogen generation from NH_(3)BH_(3) hydrolysis(Co activates NH_(3)BH_(3) and Co-C activates H_(2)O).The construction of hetero-structural catalysts provides theoretical direction for the rational design of advanced transition metal carbide materials in the field of energy catalysis and conversion.

关键词： Interfacial active sites Bimolecular activation Heterostructure Hydrogen generation Catalytic mechanism

Effect and mechanism of baicalin and geniposide on excitotoxicity of acute cerebral ischemia

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中国药理学与毒理学杂志 2018年第4期32卷 333-334页

作者： huan-huan zhang Han LIU Yuan-xue GAO Lin HE Jie WU Jing-yun XIANG Min LI Bin WANG Ya-guo KANG Shaanxi University of Traditional Chinese Medicine Xianyang 712046 China

OBJECTIVE Based on the methods of microdialysis,HPLC-MS/MS and gene chip ***,the mechanism of Baicalin and Geniposide(BC/GP) against excitatory amino acid toxicity in *** ischemia was *** will provide guidance for the clinical application of BC/GP and the study of excitatory amino acid toxicity in cerebral ***(1) Microdialysis technique and HPLC-MS/MS was performed to study the pharmacodynamics of BC/GP against cerebral ischemia.(1)18 SD rats with body weight of(280±20) g were randomly divided into control group,treatment groups with BC/CP at low dose,medium dose and high dose(equal to the dosage of crude drugs for 30 mg·kg^(-1),45 mg·kg^(-1) and 60 mg·kg^(-1) respectively).Rats in each group were given intragastric administration for seven days to establish cerebral ischemia ***,microdialysis probe was applied to collect cerebrospinal fluid from hippocampus before and after cerebral ischemia.(2) First,we established the HPLC-MS/MS method for measuring drugs and excitatory amino *** we detected the *** samples and observed their changes in animals.(2) The mechanism of BC/GP against excitatory toxicity of cerebral ischemia were observed at gene level by chip technique.(1) 16 SD rats with body weight of 240±20 g were randomly divided into sham group,model group,treatment group of BC(60 mg·kg^(-1)),treatment group of GP(60 mg·kg^(-1)) and treatment group of BC/GP(7:3)(60 mg·kg^(-1)).Rats in eachgroup were given intragastric administration for seven days to establish cerebral ischemia *** the rats were sacrificed,and the hippocampus were rapidly harvested and stored at-80℃ for further detection.(2) After the quality inspection of the hippocampal,the qualified samples were subjected to detect the levels of neurotransmitter receptor gene in the ischemic of rats by gene chip ***,the results were analyzed by the method of ΔΔ***(1) Only three compounds *** GP,glutamic acid and asp

关键词：基因芯片技术脑缺血治疗方法临床分析