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Journal of Animal Science and Biotechnology 2024年第3期15卷 971-986页

作者： Haorong He Yuanhang Wei Yuqi Chen Xiyu Zhao Xiaoxu Shen Qing Zhu huadong yin Key Laboratory of Livestock and Poultry Multi‑Omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengduSichuan 611130China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengduSichuan 611130China

Background The reproductive performance of chickens mainly depends on the development of ***-mal follicle development can lead to decreased reproductive performance and even ovarian disease among *** is the only non-human animal with a high incidence of spontaneous ovarian *** recent years,the involvement of circRNAs in follicle development and atresia regulation has been *** In the present study,we used healthy and atretic chicken follicles for circRNA *** results showed differential expression of *** was then confirmed that circRALGPS2 can translate into a protein,named cir-cRALGPS2-212aa,which has IRES ***,we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and *** Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

关键词： Apoptosis Autophagy Chicken CircRALGPS2 Follicle atresia PARP1 RNC-seq

Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-βsignaling

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Journal of Integrative Agriculture 2024年第8期23卷 2764-2777页

作者： Xiaoxu Shen Yongtong Tian Wentao He Can He Shunshun Han Yao Han Lu Xia Bo Tan Menggen Ma Houyang Kang Jie Yu Qing Zhu huadong yin Key Laboratory of Livestock and Poultry Multi-omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China College of Forestry Sichuan Agricultural UniversityChengdu 611130China College of Resources Sichuan Agricultural UniversityChengdu 611130China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China Key Laboratory for Animal Disease Resistance Nutrition of China Institute of Animal NutritionMinistry of Education/Sichuan Agricultural UniversityChengdu 611130China

MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely ***,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and ***,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing ***,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of ***1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell ***,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken ***2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 *** findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.

关键词： miRNA-181-5p family SMSCs differentiation TGFBR1 TGF-βsignaling

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高地应力下深部硐室底鼓破坏围岩稳定性分析

防灾减灾工程学报 2024年第3期44卷 660-669页

作者：张道兵尹华东胡阿平朱远嫘彭学军张升安方信湖南科技大学资源环境与安全工程学院湖南湘潭411201 中铁五局集团第一工程有限责任公司湖南长沙410117

为研究西部山区高地应力作用下深部硐室的稳定性问题,根据普氏压力拱理论,综合考虑深部硐室冒顶,片帮以及底鼓破坏,建立了高地应力下深部硐室底鼓破坏模式,基于极限分析上限法和非线性Hoek-Brown破坏准则,推导了该破坏模式下深部硐室的... 详细信息

为研究西部山区高地应力作用下深部硐室的稳定性问题,根据普氏压力拱理论,综合考虑深部硐室冒顶,片帮以及底鼓破坏,建立了高地应力下深部硐室底鼓破坏模式,基于极限分析上限法和非线性Hoek-Brown破坏准则,推导了该破坏模式下深部硐室的围岩压力理论公式,求解了该破坏模式下深部硐室围岩压力上限解,并将本文结果与数值模拟和已有成果进行对比分析。此外,通过该破坏模式研究了各参数对围岩压力和潜在破坏面的影响。结果表明:(1)随着初始地应力场参数中σ_(V)和λ增大,深部地下硐室围岩压力q逐渐增大,硐室围岩潜在破坏面逐渐增大,其中σ_(V)表现尤为显著;(2) Hoek-Brown破坏准则参数中GSI、σ_(ci)和m_(i)的增大对深部硐室围岩稳定性有明显的提高效应,而D增加则会降低硐室围岩的稳定性;(3)随着底部压力相关系数μ增加,深部地下硐室顶板和两帮的围岩压力表现为减小的趋势。研究成果可为深部硐室的支护设计提拱有效的理论支撑。

关键词：高地应力硐室底鼓破坏模式围岩压力 Hoek-Brown破坏准则

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肥胖对体外冲击波治疗膝骨关节炎疗效的影响

中国医学前沿杂志（电子版） 2024年第6期16卷 38-43页

作者：刘彧邬春虎吴坤邢更彦胡晓华张联合陈长松尹华东马玉海武警浙江省总队医院骨科浙江杭州310051 北京大学第三医院骨科北京100191 中国人民解放军总医院第三医学中心骨科北京100080

目的探讨肥胖对体外冲击波治疗(extracorporeal shockwave therapy,ESWT)膝骨关节炎(knee osteoarthri-tis,KOA)疗效的影响。方法选取2021年1月至2022年12月于武警浙江省总队医院进行ESWT的123例KOA患者,其中男性38例,女性85例;年龄(59.36±5.69)岁;病程(14.92±6.9)个月。患者根据体质量指数(body mass in-dex,BMI)分为正常组(BMI<25 kg/m^(2))和肥胖组(BMI≥25 kg/m^(2))。所有患者接受ESWT,每周1次,连续治疗8周。比较两组治疗前,治疗第2周后及第8周后患者的视觉模拟评分法(Visual Analogue Scale,VAS)、膝骨性关节炎功能指数(Lequesne评分)及西大略和麦克马斯特大学骨关节炎指数(McMaster University Osteoarthritis Index,WOMAC)评分,以评价患者疼痛和功能改善情况。结果两组患者治疗前VAS评分差异无统计学意义,治疗第2周后肥胖组VAS疼痛评分疼痛缓解优于正常组[(2.40±0.79)分比(3.28±1.17)分,P<0.01];肥胖组Lequesne评分改善优于正常组[(4.40±2.54)分比(5.81±2.58)分,P<0.01];肥胖组WOMAC评分中的疼痛评分、功能评分改善优于正常组[(6.76±2.58)比(7.98±2.24),P<0.01;(21.14±8.59)比(25.91±8.09),P<0.01]。治疗第8周后,VAS疼痛评分显示与正常组相比,肥胖组疼痛改善明显[(1.63±0.85)分比(2.27±1.44)分,P<0.01];Lequesne评分显示治疗第8周后与正常组相比,肥胖组患者治疗后功能改善明显[(3.09±1.68)分比(4.47±2.76)分,P<0.01];WOM-AC评分中疼痛评分、僵硬评分、功能评分治疗第8周后正常组为(5.00±2.74)、(2.54±1.15)、(22.25±8.17)分,与肥胖组(3.27±1.78)、(2.17±0.75)、(18.45±5.84)分相比,肥胖组患者治疗第8周后疼痛及功能改善明显(P<0.05),在治疗第2周后、第8周后,WOMAC评分中疼痛评分结果与VAS评分结果一致,疼痛明显减轻,WOMAC评分中的功能评分与Lequesne功能评分相一致,功能明显改善。结论ESWT可以早期缓解患者的疼痛及功能,早期治疗效果肥胖患者优于正常体重患者;在ESWT治疗过程中,对于肥胖的患者,ESWT对于膝骨关节炎的治疗效果优于正常体重患者;肥胖可以影响KOA发生发展,但是不影响ESWT对KOA的治疗,相较正常体重患者,肥胖患者更有利于ESWT对KOA的治疗。

关键词：肥胖骨关节炎膝关节体外冲击波

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A Non-canonical Excitatory PV RGC–PV SC Visual Pathway for Mediating the Looming-evoked Innate Defensive Response

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Neuroscience Bulletin 2024年第3期40卷 310-324页

作者： Man Yuan Sen Jin Gao Tan Siyuan Song Yizong Liu huadong Wang yin Shen Eye Center Renmin Hospital of Wuhan UniversityWuhan UniversityWuhan430060China The Brain Cognition and Brain Disease Institute Shenzhen Key Laboratory of Viral Vectors for BiomedicineShenzhen Institute of Advanced TechnologyChinese Academy of Sciences(CAS)Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research InstitutionsNational Medical Products Administration Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsShenzhen Key Laboratory of Quality Control Technology for Virus-Based TherapeuticsGuangdong Provincial Medical Products AdministrationShenzhen518055China Jan and Dan Duncan Neurological Research Institute Baylor College of MedicineHouston77030USA State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics Key Laboratory of Magnetic Resonance in Biological SystemsWuhan Center for Magnetic ResonanceInnovation Academy for Precision Measurement Science and TechnologyCASWuhan430071China Frontier Science Center of Immunology and Metabolism Medical Research InstituteWuhan UniversityWuhan430071China

Parvalbumin-positive retinal ganglion cells(PV+RGCs)are an essential subset of RGCs found in various ***,their role in transmitting visual information remains ***,we characterized PV+RGCs in the retina and explored the functions of the PV+RGC-mediated visual *** applying multiple viral tracing strategies,we investigated the downstream of PV+RGCs across the whole ***,we found that the PV+RGCs provided direct monosynaptic input to PV+excitatory neurons in the superficial layers of the superior colliculus(SC).Ablation or suppression of SC-projecting PV+RGCs abolished or severely impaired the flight response to looming visual stimuli in mice without affecting visual ***,using transcriptome expression profiling of individual cells and immunofluorescence colocalization for RGCs,we found that PV+RGCs are predominant glutamatergic ***,our findings indicate the critical role of PV+RGCs in an innate defensive response and suggest a non-canonical subcortical visual pathway from excitatory PV+RGCs to PV+SC neurons that regulates looming visual *** results provide a potential target for intervening and treating diseases related to this circuit,such as schizophrenia and autism.

关键词： Parvalbumin-positive retinal ganglion cell Innate fear Superior colliculus Excitatory-excitatory neuronal connection Looming-evoked defensive response Subcortical pathway

不同公母比对本交笼模式下种公鸡性行为影响的研究

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中国畜禽种业 2024年第6期20卷 54-60页

作者：张姚陈玉琪杜金花齐莎日娜李文艳朱庆尹华东四川农业大学四川成都611130 四川圣迪乐村生态食品股份有限公司四川绵阳622150 四川省盐亭县农业农村局四川盐亭621600

为探究本交笼养模式下种公鸡的行为学特征,了解本交笼种公鸡的行为习惯。该试验在本交笼模式下选择594只50周龄的罗曼粉蛋种鸡,随机分为2组,每组3个重复,每个重复99只鸡,试验1、 2组公母比分别为1∶8和1∶10,连续1周观察各处理组种公鸡... 详细信息

为探究本交笼养模式下种公鸡的行为学特征,了解本交笼种公鸡的行为习惯。该试验在本交笼模式下选择594只50周龄的罗曼粉蛋种鸡,随机分为2组,每组3个重复,每个重复99只鸡,试验1、 2组公母比分别为1∶8和1∶10,连续1周观察各处理组种公鸡的行为,并进行记录和分类统计。结果表明:本交笼养蛋种鸡的交配活动显著发生在下午,同时试验组1鸡只交配频率显著高于试验组2 (P0.05)。对种公鸡的等级进行划分,试验组1等级地位较高的种公鸡体型偏小,而试验组2等级地位较高的种公鸡体型则偏大;剔除笼内无效种公鸡后,种蛋受精率显著提高(P<0.05)。可见,试验组1种公鸡的均匀度和配种能力要优于试验组2。

关键词：本交笼种公鸡交配行为公母比

miRNA sequencing analysis of healthy and atretic follicles of chickens revealed that miR-30a-5p inhibits granulosa cell death via targeting Beclin1

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Journal of Animal Science and Biotechnology 2022年第5期13卷 1351-1372页

作者： Haorong He Dongmei Li Yongtong Tian Qinyao Wei Felix Kwame Amevor Congjiao Sun Chunlin Yu Chaowu Yang Huarui Du Xiaosong Jiang Menggen Ma Can Cui Zhichao Zhang Kai Tian Yao Zhang Qing Zhu huadong yin Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130SichuanChina National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and ReproductionMinistry of Agriculture and Rural AffairsCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Animal Breeding and Genetics key Laboratory of Sichuan Province Sichuan Animal Science AcademyChengdu 610066China College of Resources Sichuan Agricultural UniversityChengdu 611130China

Background:The egg production performance of chickens is affected by many factors,including genetics,nutrition and environmental *** factors all play a role in egg production by affecting the development of ***(miRNAs)are important non-coding RNAs that regulate biological processes by targeting genes or other non-coding RNAs after *** the animal reproduction process,miRNA is known to affect the development and atresia of follicles by regulating apoptosis and autophagy of granulosa cells(GCs).Results:In this study,we identified potential miRNAs in the atretic follicles of broody chickens and unatretic follicles of healthy *** identified gga-miR-30a-5p in 50 differentially expressed miRNAs and found that gga-miR-30a-5p played a regulatory role in the development of chicken *** function of miR-30a-5p was explored through the transfection test of miR-30a-5p inhibitor and miR-30a-5p *** the study,we used qPCR,western blot and flow cytometry to detect granulosa cell apoptosis,autophagy and steroid hormone *** microscopy and transmission electron microscopy are used for the observation of *** levels of estradiol(E2),progesterone(P4),malondialdehyde(MDA)and superoxide dismutase(SOD)were detected by *** results showed that miR-30a-5p showed a negative effect on autophagy and apoptosis of granulosa cells,and also contributed in steroid hormones and reactive oxygen species(ROS)*** addition,the results obtained from the biosynthesis and dual luciferase experiments showed that Beclin1 was the target gene of *** rescue experiment conducted further confirmed that Beclin1 belongs to the miR-30a-5p regulatory ***:In summary,after deep miRNA sequencing on healthy and atretic follicles,the results indicated that miR-30a-5p inhibits granulosa cell death by inhibiting Beclin1.

关键词： Apoptosis Autophagy Beclin1 Chicken granulosa cells MiR-30a-5p Oxidative stress RNA-seq Steroid hormone

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孔隙水作用下深埋硐室稳定性分析及破坏机制

中国安全科学学报 2023年第10期33卷 62-70页

作者：张道兵朱远嫘尹华东胡阿平汤宇湖南科技大学资源环境与安全工程学院湖南湘潭411201 中铁五局集团第一工程有限责任公司湖南长沙410117

为获得孔隙水作用下深埋硐室的围岩压力及其潜在破坏面,将Hoek-Brown破坏准则和极限分析定理结合,改进传统的深埋硐室“楔形体”破坏机制,并将孔隙水作用考虑到优化的深埋硐室围岩稳定性计算模型中;然后根据虚功率原理推导出孔隙水作用... 详细信息

为获得孔隙水作用下深埋硐室的围岩压力及其潜在破坏面,将Hoek-Brown破坏准则和极限分析定理结合,改进传统的深埋硐室“楔形体”破坏机制,并将孔隙水作用考虑到优化的深埋硐室围岩稳定性计算模型中;然后根据虚功率原理推导出孔隙水作用下深埋硐室围岩压力解析解,通过序列二次规划(SQP)算法优化得到围岩压力上限解以及拱顶、拱肩破坏范围。研究表明:Hoek-Brown准则参数中地质强度指数(GSI)、岩体常数、岩体单轴抗压强度增大时围岩压力减小,硐室的潜在塌落范围也减小,而扰动因子、岩体容重和硐室直径的增大会对硐室的稳定性产生不利影响。此外,孔隙水压力系数增大时围岩压力和塌落范围也不断增大,且随着水位线高度的增加,影响程度越加明显。

关键词：孔隙水深埋硐室围岩稳定性围岩压力破坏机制 Hoek-Brown破坏准则

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对国内外翻转课堂热的冷思考:实证与反思

民族教育研究 2016年第1期27卷 25-30页

作者：尹华东首都师范大学大学英语教研部北京100048

本文回顾了翻转课堂的起源和发展历程,分析了其研究现状及存在的主要问题,探讨了大学英语翻转课堂实验研究结果。结果显示翻转课堂在增进学习兴趣方面效果明显,但对增强学习动机作用有限,能否显著提升学习成绩还需进一步论证。本研究说... 详细信息

本文回顾了翻转课堂的起源和发展历程,分析了其研究现状及存在的主要问题,探讨了大学英语翻转课堂实验研究结果。结果显示翻转课堂在增进学习兴趣方面效果明显,但对增强学习动机作用有限,能否显著提升学习成绩还需进一步论证。本研究说明翻转课堂有一定积极意义,但也有自身局限性,由于对翻转课堂的认识还不够全面,需冷静面对翻转课堂"热潮",理性看待其作用和价值。

关键词：翻转课堂学习成绩学习兴趣学习动机

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试论知识经济下的21世纪国企管理

煤炭经济研究 2000年第4期20卷 43-46页

作者：殷华东大屯煤电(集团)有限责任公司

新的经济形式———知识经济或者是后工业经济来临是必然的。不管最终以什么样的面目出现 ,一些基本的特征和要求已经确定无疑。因此 ,作为知识创新经济活动主体的企业 ,必须未雨绸缪。对于国有企业来说 ,由于受传统管理影响很大 ,更应... 详细信息

新的经济形式———知识经济或者是后工业经济来临是必然的。不管最终以什么样的面目出现 ,一些基本的特征和要求已经确定无疑。因此 ,作为知识创新经济活动主体的企业 ,必须未雨绸缪。对于国有企业来说 ,由于受传统管理影响很大 ,更应该早做准备 ,从观念、体制、最终是行动上 ,去实现管理的变革和创新。当然 ,国有企业管理是个系统问题 ,需要政府、企业以及每个参与者的共同努力 ,不可能一蹴而就 ,因此 ,希望有更多的人来关注和研究。

关键词： 21世纪知识经济国有企业经营管理