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A single silk-and multiple pollen-expressed PMEs at the Ga1 locus modulate maize unilateral cross-incompatibility

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Journal of Integrative Plant Biology 2023年第5期65卷 1344-1355页

作者： Zhaogui Zhang Kai Li Huairen Zhang Qiuxia Wang Li Zhao Juan Liu huabang chen State Key Laboratory of Plant Cell and Chromosome Engineering Innovative Academy of Seed DesignInstitute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

The Gametophyte factor1(Ga1) locus in maize confers unilateral cross-incompatibility(UCI), and it is controlled by both pollen and silk-specific determinants. Although the Ga1 locus has been reported for more than a century and is widely utilized in maize breeding programs, only the pollen-specific ZmGa1P has been shown to function as a male determinant;thus, the genomic structure of the Ga1 locus and all the determinants that control UCI at this locus have not yet been fully characterized.Here, we used map-based cloning to confirm the determinants of UCI at the Ga1 locus and maize pan-genome sequence data to characterize the genomic structure of the Ga1 locus. The Ga1 locus comprises one silk-expressed pectin methylesterase gene(PME, ZmGa1F) and eight pollenexpressed PMEs(ZmGa1P and ZmGa1PL1-7).Knockout of ZmGa1F in Ga1/Ga1 lines leads to the complete loss of the female barrier function. The expression of individual ZmGa1PL genes in a ga1/ga1 background endows ga1 pollen with the ability to overcome the female barrier of the Ga1 locus. These findings, combined with genomic data and genetic analyses, indicate that the Ga1 locus is modulated by a single female determinant and multiple male determinants, which are tightly linked.The results of this study provide valuable insights into the genomic structure of the Ga2 and Tcb1 loci and will aid applications of these loci in maize breeding programs.

关键词： Gametophyte factor1 maize unilateral cross-incompatibility ZmGa1F ZmGa1PL

ORF355 confers enhanced salinity stress adaptability to S-type cytoplasmic male sterility maize by modulating the mitochondrial metabolic homeostasis

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Journal of Integrative Plant Biology 2023年第3期65卷 656-673页

作者： Senlin Xiao Wei Song Jinfeng Xing Aiguo Su Yanxin Zhao Chunhui Li Zi Shi Zhiyong Li Shuai Wang Ruyang Zhang Yuanrong Pei huabang chen Jiuran Zhao Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Maize Research InstituteBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China State Key Laboratory of Plant Cell and Chromosome Engineering Innovative Academy of Seed DesignInstitute of Genetics and Developmental Biologythe Chinese Academy of SciencesBeijing 100101China

Moderate stimuli in mitochondria improve wideranging stress adaptability in animals, but whether mitochondria play similar roles in plants is largely unknown. Here, we report the enhanced stress adaptability of S-type cytoplasmic male sterility(CMS-S) maize and its association with mild expression of sterilizing gene ORF355. A CMS-S maize line exhibited superior growth potential and higher yield than those of the near-isogenic N-type line in saline fields. Moderate expression of ORF355 induced the accumulation of reactive oxygen species and activated the cellular antioxidative defense system. This adaptive response was mediated by elevation of the nicotinamide adenine dinucleotide concentration and associated metabolic homeostasis. Metabolome analysis revealed broad metabolic changes in CMS-S lines, even in the absence of salinity stress. Metabolic products associated with amino acid metabolism and galactose metabolism were substantially changed, which underpinned the alteration of the antioxidative defense system in CMS-S plants. The results reveal the ORF355-mediated superior stress adaptability in CMS-S maize and might provide an important route to developing salt-tolerant maize varieties.

关键词： cytoplasmic male sterility metabolic homeostasis mitochondria salinity stress adaptability Zea mays L.

Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

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Journal of Genetics and Genomics 2017年第9期44卷 465-468页

作者： Jun Li Huawei Zhang Xiaomin Si Youhui Tian Kunling chen Jinxing Liu huabang chen Caixia Gao State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental Biology Chinese Academy of Sciences University of Chinese Academy of Sciences State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Maize（Zea mays L.）is one of the most important cereal crops,with a global production of 1.02 billion tons in 2013（Baldaufa et al.,2016）.Heterosis is widely used to increase the productivity of maize,and the first ... 详细信息

关键词： PCR RE Generation of thermosensitive male-sterile maize by targeted knockout of the ZmTMS5 gene

Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize

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Molecular Plant 2020年第9期13卷 1270-1283页

作者： Senlin Xiao Jie Zang Yuanrong Pei Jie Liu Juan Liu Wei Song Zi Shi Aiguo Su Jiuran Zhao huabang chen State Key Laboratory of Plant Cell and Chromosome Engineer ngInnovative Academy of Seed DesignInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100864China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Maize Research CenterBeijing Academy of Agriculture and Forestry SciencesBeijing100097China

Coordination between mitochondria and the nucleus is crucial for fertility determination in plants with cytoplasmic male sterility(CMS).Using yeast one-hybrid screening,we identified a transcription factor,ZmDREB1.7,that is highly expressed in sterile microspores at the large vacuole stage and activates the expression of mitochondria-encoded CMS gene orf355.Δpro,a weak allele of ZmDREB1.7 with the loss of a key unfolded protein response(UPR)motif in the promoter,partially restores male fertility of CMS-S maize.ZmDREB1.7 expression increases rapidly in response to antimycin A treatment,but this response is attenuated in theΔpro allele.Furthermore,we found that expression of orf355 in mitochondria activates mitochondrial retrograde signaling,which in turn induces ZmDREB1.7 expression.Taken together,these findings demonstrate that positive-feedback transcriptional regulation between a nuclear regulator and a mitochondrial CMS gene determines male sterility in maize,providing new insights into nucleus-mitochondria communication in plants.

关键词： maize cytoplasmic male sterility transcription factor nucleus-mitochondria communication

Immature Pollen Exine(IPE) is required for pollen wall development in maize

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Immature Pollen Exine(IPE) is required for pollen wall devel...

The Genetic and Molecular Mechanisms of the Ga1 Locus Underlying Unilateral Cross-incompatibility in Maize

2016年全国植物生物学大会

作者： Youhui Tian Senlin Xiao huabang chen State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Exine,the outer layer of pollen wall,is more structured than any other plant walls due to its vital role in protecting genetic material from hostile environment. It is composed of sporopollenin,an undeciphered complicated lipidic compound. Tapetum,the innermost layer of sporophytic anther wall,serves as the provider for sporopollenin precursor. We reported here a male sterile mutant immature pollen exine(ipe) in maize. It showed shrunk anther and immature pollen wall at large vacuole stage. We cloned the gene by map based cloning. It encoded a fatty acyl carrier protein(ACP) reductase which was localized in plasmid mediated by N-terminal transit peptide. Expression analysis indicated that IPE was expressed mainly in tapetum and microspore after the microspore was released from tetrad. Functional complementation of homologous Arabidopsis mutant demonstrated that IPE is conserved in monocot and dicot,and may even in flowering plants.

关键词： male sterile exine IPE tapetum maize

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The Genetic and Molecular Mechanisms of the Ga1 Locus Underl...

船舶铝空气电池-锂离子电池混合动力系统能量管理策略研究

2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： chen huabang

＜正＞Maize gametophyte factor1 （Ga1） has been discovered in the early 1900s and represents a unique unilateral cross-incompatibility （UCI） system. Although Ga1 has been studied and utilized in maize breeding for about a century, the mechanism underlying maize UCI specificity determination remains unknown. The major reason for this is the complexity of this locus, making cloning of the UCI determinants a daunting challenge. The maize Ga1 locus is constituted of three different haplotypes, in which Ga1-S haplotype

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储能科学与技术 2023年第9期12卷 2871-2880页

作者：张华榜陈月李钦王红强秦国锋吴强李庆余广西师范大学化学与药学学院广西新能源船舶电池工程技术研究中心广西师范大学职业技术师范学院广西桂林541000 安徽益佳通电池有限公司安徽宣城242000

将铝空气电池与锂离子电池组成混合动力系统,分析不同拓扑结构的优缺点,确定混合动力系统的拓扑结构,对其系统原理进行了设计,完成了系统关键零部件的选型参数,搭建了混合动力系统的试验平台。为了提升铝空气电池的效率和锂离子电池使... 详细信息

将铝空气电池与锂离子电池组成混合动力系统,分析不同拓扑结构的优缺点,确定混合动力系统的拓扑结构,对其系统原理进行了设计,完成了系统关键零部件的选型参数,搭建了混合动力系统的试验平台。为了提升铝空气电池的效率和锂离子电池使用寿命,提出了一种基于规则控制的能量管理策略,该策略主要考虑了锂离子电池的SOC和负载功率变化,使铝空气电池在大功率范围恒功率放电,锂离子电池变功率工作,通过试验测试验证该策略的有效性。

关键词：铝空气电池混合动力系统能量管理策略规则控制

转基因玉米目标基因纯合体快速准确的PCR鉴定方法(英文)

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分子植物育种 2009年第3期7卷 619-623页

作者：刘楠陈化榜山东农业大学作物生物学国家重点实验室泰安271018

本文以转基因玉米NK603为例,介绍了一种检测目标基因纯和体的PCR方法。该方法利用4个引物的多重PCR反应,这4个PCR引物分别来自于目标基因的5'端(5'TP)和3'端(3'TP)DNA序列,目标基因5'端一侧的玉米基因组DNA序列(5'GP),以及目标基因3'端... 详细信息

本文以转基因玉米NK603为例,介绍了一种检测目标基因纯和体的PCR方法。该方法利用4个引物的多重PCR反应,这4个PCR引物分别来自于目标基因的5'端(5'TP)和3'端(3'TP)DNA序列,目标基因5'端一侧的玉米基因组DNA序列(5'GP),以及目标基因3'端一侧的玉米基因组DNA序列(3'GP)。视玉米个体有无目标基因以及目标基因的杂合/纯和状态,PCR扩增可得到三种不同的结果:如果被检测个体无目标基因,5'GP与3'GP间的玉米基因组DNA将被扩增,PCR只产生一条条带;如果被检测个体是目标基因的纯合体,5'GP与5'TP及3'TP与3'GP间的DNA将被扩增,PCR则产生两条条带;如果被检测个体是目标基因的杂合体,5'GP与3'GP、5'GP与5'TP以及3'TP与3'GP间的DNA将都被扩增,PCR则产生三条条带。三条不同长短的PCR产物条带在琼脂糖凝胶上清晰可分,易于辨别。和费时昂贵的定量PCR相比,该方法简单、快速、结果准确,在目标基因位点玉米基因组DNA序列已知的前提下,该方法可扩展到诸如Bt11、Event176、GA21、MON810、MON863 和 TC1507 等任何转基因玉米的回交转育程序中。

关键词：转基因玉米 NK603 纯合性检测多重PCR

2,6-二甲基苯酚催化胺化过程Pd/γ-Al_2O_3催化剂的失活和改性

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石油学报（石油加工） 2012年第4期28卷 571-576页

作者：王化邦马建超吴志威陈立功李阳天津大学化工学院天津300072

采用固定床反应器,研究了Pd/γ-Al2O3催化剂在催化2,6-二甲基苯酚气相胺化合成2,6-二甲基苯胺反应中的稳定性,实验结果发现该催化剂较易失活。采用BET、XPS、TG、TEM和IR手段对失活前后的Pd/γ-Al2O3催化剂进行了表征分析,并采用BaO对Pd... 详细信息

采用固定床反应器,研究了Pd/γ-Al2O3催化剂在催化2,6-二甲基苯酚气相胺化合成2,6-二甲基苯胺反应中的稳定性,实验结果发现该催化剂较易失活。采用BET、XPS、TG、TEM和IR手段对失活前后的Pd/γ-Al2O3催化剂进行了表征分析,并采用BaO对Pd/γ-Al2O3进行改性。结果表明,Pd/γ-Al2O3催化剂失活的原因主要是反应过程生成的胺类化合物吸附在γ-Al2O3载体的酸性中心产生积炭沉积所致。Pd/γ-Al2O3催化剂添加BaO,生成了铝钡尖晶石结构,有效地减少了Pd/γ-Al2O3的酸性,加快了反应中生成的2,6-二甲基环己胺和2,6-二甲基苯胺的脱附,有效地避免了催化剂因积炭失活,提高了催化剂的稳定性。

关键词： Pd/γ-Al2O3 2,6-二甲基苯酚胺化失活改性