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Coupling WO_(3-x) dots-encapsulated metal-organic frameworks and template-free branched polymerization for dual signal-amplified electrochemiluminescence biosensing

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Chinese Chemical Letters 2024年第4期35卷 469-474页

作者： Fei Yin Erli Yang Xue Ge Qian Sun Fan mo Guoqiu wu Yanfei Shen Medical School Southeast UniversityNanjing 210009China Center of Clinical Laboratory Medicine Zhongda HospitalSoutheast UniversityNanjing 210009China Jiangsu Provincial Key Laboratory of Critical Care Medicine Southeast UniversityNanjing 210009China

Developing accurate and sensitive DNA methyltransferase(MTase) analysis methods is essential for early clinical diagnosis and development of antimicrobial drug targets. In this work, by coupling WO_(3-x) dotsencapsulated metal-organic frameworks(moFs) as co-reactants and terminal deoxynucleotidyl transferase(Td T)-mediated template-free branched polymerization, a dual signal-amplified electrochemiluminescent(ECL) biosensor was constructed to detect DNA adenine methylation(Dam) MTase. The employment of WO_(3-x) dots-encapsulated moFs(i.e., NH_(2)-UIO66@WO_(3-x) ) was not only beneficial for biomolecule conjugation because of the abundant amino groups but also led to a 7-fold enhanced ECL response due to the increased loading of WO_(3-x). moreover, Td T-mediated template-free branched polymerization promoted the capture of ECL emitters on the electrode surface, achieving 20-fold enhanced signal amplification. The presented ECL biosensor demonstrated a low detection limit of 2.4 × 10^(-4)U/m L, and displayed high reliability for the detection of Dam MTase in both spiked human serum and E. coli cell samples, and for the screening of potential inhibitors. This study opens a new avenue for designing a dual signal amplificationbased ECL bioassay for Dam MTase and screening inhibitors in the fields of clinical diagnosis and drug development.

关键词： Dual signal-amplified electrochemiluminescence WO_(3-x)dots moFs Template-free branched polymerization DNA methyltransferase

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Accelerated aging and frailty in cardiovascular diseases

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Journal of Geriatric Cardiology 2024年第8期21卷 828-830页

作者： Xue-hua CHENG Tao wu Li HAN Department of Traditional Chinese Medicine Geriatrics Huadong Hospital Affiliated to Fudan UniversityShanghaiChina

Aging is the greatest risk factor for cardiovascular disease(CVD),which is the most common cause of death in European countries.[1]It is estimated that during the period from 2020 to 2022,there were 9.0%more excess deaths from CVD than expected.[2]By 2030,approximately 20%of the population will be aged 65 years and older,and CVD will account for 40%of deaths in this age group,[3]imposing a significant burden on the healthcare system and society as a *** is related to frailty,which represents the dynamic progression of physical and physiological decline in older *** limits an individual’s ability to recover from acute stressors and serves as a predictor of clinical adverse *** the uncertainties in screening,assessing,and managing frailty in clinical practice,emphasizing the connections between frailty,aging,and CVD will provide new insights for the prediction,prevention and management of CVD in the aging population.

关键词： aging prevention prediction

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Identification and Characteristics Analysis of Micro-Seismic Signals in the Haima Seep Area

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Journal of Earth Science 2024年第1期35卷 288-291页

作者： Xiangchun Wang Bing Nie Zhiyu wu Weiwei Wang hua Chen China University of Geosciences Beijing 100083China CNOOC China Limited Shenzhen BranchShenzhen 518000China Guangzhou Marine Geological Survey Guangzhou 511458China CNOOC China Limited Shanghai BranchShanghai 200335China

*** recent years,with the steady development of offshore energy exploration technology,submarine gas seepage sites,and their products are gradually being identified and *** submarine cold seep is a seepage phenomenon in which hydrocarbon gases stored deep in the seafloor are transported to seawater by gushing or seepage under the action of tectonic compression(Roy et al.,2019;Tinivella and Giustiniani,2016).

关键词： submarine Seismic offshore

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Experimental investigation on using CO_(2)/H_(2)O emulsion with high water cut in enhanced oil recovery

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Petroleum Science 2024年第2期21卷 974-986页

作者： Xi-Dao wu Peng Xiao Bei Liu Guang-Jin Chen Jian-hua Pang State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing102249China Ocean Intelligence Technology Center Shenzhen Institute of Guangdong Ocean UniversityShenzhen518120GuangdongChina

CO_(2) emulsions used for EOR have received a lot of interest because of its good performance on CO_(2)mobility ***,most of them have been focusing on the high quality CO_(2) emulsion(high CO_(2) fraction),while CO_(2) emulsion with high water cut has been rarely *** this paper,we carried out a comprehensive experimental study of using high water cut CO_(2)/H_(2)O emulsion for enhancing oil ***,a nonionic surfactant,alkyl glycosides(APG),was selected to stabilize CO_(2)/H_(2)O emulsion,and the corresponding morphology and stability were evaluated with a transparent PVT ***,plugging capacity and apparent viscosity of CO_(2)/H_(2)O emulsion were measured systematically by a sand pack displacement apparatus connected with a 1.95-m long capillary ***,a high water cut(40 vol%) CO_(2)/H_(2)O emulsion was selected for flooding experiments in a long sand pack and a core sample,and the oil recovery,the rate of oil recovery,and the pressure gradients were *** results indicated that APG had a good performance on emulsifying and stabilizing CO_(2) *** inversion from H_(2)O/CO_(2) emulsion to CO_(2)/H_(2)O emulsion with the increase in water cut was ***_(2)/H_(2)O emulsions with lower water cuts presented higher apparent viscosity,while the optimal plugging capacity of CO_(2)/H_(2)O emulsion occurred at a certain water ***,the displacement using CO_(2)/H_(2)O emulsion provided 18.98% and 13.36% additional oil recovery than that using pure CO_(2) in long sand pack and core tests,*** work may provide guidelines for EOR using CO_(2) emulsions with high water cut.

关键词： CO_(2)/H_(2)O emulsion High water cut CO_(2) mobility control Enhanced oil recovery

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Ganoderma lucidum:a comprehensive review of phytochemistry,efficacy,safety and clinical study

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Food Science and Human Wellness 2024年第2期13卷 568-596页

作者： Sijia wu Siyuan Zhang Bo Peng Dechao Tan Mingyue wu Jinchao Wei Yitao Wang hua Luo Macao Centre for Research and Development in Chinese Medicine State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical SciencesUniversity of MacaoMacao SAR 999078China College of Pharmacy Guangxi Medical UniversityNanning 530021China Guangxi University of Chinese Medicine Nanning 530001China

Ganoderma lucidum,one of the most well-known edible fungi,is believed to be very beneficial for longevity and vitality.A long usage history suggests that *** has various clinical therapeutic *** experimental studies have confirmed that *** has multiple pharmacological effects,including antitumor,anti-microbial,anti-HIV protease,and antidiabetic activity and so *** the deepening of research,more than 300 compounds have been isolated from *** is an increasing population of ***-based products,and its international development is ***,*** has drawn much attention to its chemical composition,therapeutic effect,clinical value,and *** paper provides a comprehensive review of these aspects to enhance the global promotion of ***.

关键词： Ganoderma lucidum Phytochemistry Efficacy Safety Clinical study

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The RNA-binding domain of DCL3 is required for long-distance RNAi signaling

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aBIOTECH 2024年第1期5卷 17-28页

作者： Jie Li Bo-Sen Zhang hua-Wei wu Cheng-Lan Liu Hui-Shan Guo Jian-hua Zhao State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBeijing 100049China Qilu Zhongke Academy of modern Microbiology Technology Jinan 250022China

Small RNA(sRNA)-mediated RNA silencing(also known as RNA interference,or RNAi)is a conserved mechanism in eukaryotes that includes RNA degradation,DNA methylation,heterochromatin formation and protein translation *** plants,sRNAs can move either cell-to-cell or systemically,thereby acting as mobile silencing signals to trigger noncell autonomous ***,whether and what proteins are also involved in noncell autonomous silencing have not been *** this study,we utilized a previously reported inducible RNAi plant,PDSi,which can induce systemic silencing of the endogenous PDS gene,and we demonstrated that DCL3 is involved in systemic PDS silencing through its RNA binding *** confirmed that the C-terminus of DCL3,including the predicted RNA-binding domain,is capable of binding short *** affecting RNA binding,but not processing activity,reduced systemic PDS silencing,indicating that DCL3 binding to RNAs is required for the induction of systemic *** mosaic virus infection assays showed that the RNA-binding activity of DCL3 is required for antiviral RNAi in systemically noninoculated *** findings demonstrate that DCL3 acts as a signaling agent involved in noncell autonomous silencing and an antiviral effect in addition to its previously known function in the generation of 24-nucleotide sRNAs.

关键词： RNAi signaling DCL3 RBD RNAs Cucumber mosaic virus

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PACS gene family-related neurological diseases: limited genotypes and diverse phenotypes

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World Journal of Pediatrics 2024年第1期20卷 82-91页

作者： Han Zhang Kai Gao Shuang Wang Yue-hua Zhang Zhi-Xian Yang Ye wu Yu-wu Jiang Department of Pediatrics Peking University First Hospital.No.1 Xi'an Men StreetWest DistrictBeijing 100034China Bejing Key Laboratory of molecular Diagnosis and Study on Pediatric Genetic Diseases BeijingChina Children Epilepsy Center Peking University First HospitalBeijingChina Key Laboratory for Neuroscience Ministry of Education/National Health and Family Planning CommissionPeking UniversityBeijingChina Center of Epilepsy Beijing Institute for Brain DisordersBeijingChina

Background The PACS gene family has been demonstrated to be related to intracellular vesicular *** phenotypic manifestations caused by the pathogenic variants of PACS include epilepsy,intellectual disability/developmental delay,and malformations,such as facial *** We identified seven new cases with pathogenic or likely pathogenic PACS variants using next-generation *** information obtained from these patients was analyzed along with that obtained from previously reported *** With the inclusion of the newly diagnosed cases in this study,103 cases with PACS gene family-related neurological diseases were reported,of which 43 were PACS2-related cases and the remaining were PACSI-related *** patients had seizures,which have been reported to be effectively controlled by several types of anti-seizure medications(ASMs).The most efficacious and frequently prescribed ASMs included sodium valproate(43.3%,13/30),oxcarbazepine/carbamazepine(26.7%,8/30),and levetiracetam(20%,6/30).Almost all patients had intellectual disability/developmental *** most common pathogenic missense variants were PACSI ***203Trp and PACS2 *** addition,we report a patient carrying a likely pathogenic copy number variation(CNV)(de novo heterozygous deletion of chr14:105821380-106107443,286 kilobase,destroyed part of the furin-binding region domain and the protein structure after it)with more severe and refractory late-onset *** The clinical phenotypes of the different PACS heterozygous missense variants were *** pathogenic variant sites of PACSI and PACS2 were quite limited but located in different regions.A CNV destroying part of the PACS2 gene might also be *** findings may provide an important clue for further functional studies on the pathogenic mechanism of neurological disorders related to the PACS gene family.

关键词： Developmental delay Epilepsy Intellectual disability PACSI PACS2

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One-Pot Stereoselective Synthesis of Different Fused Multicyclic Iminosugars Based on the Iminium-lon Intermediate

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Chinese Journal of Chemistry 2024年第2期42卷 142-150页

作者： Song Xie Jilai wu Likai Zhou Chao Wei Xiaoliu Li hua Chen Key Laboratoryof Chemical Biology of Hebei Province College of Chemistry and Material ScienceHebei UniversityBaodingHebei 071002China College of Chemistry and Chemical Engineering Xingtai UniversityXingtaiHebei 054001China

Different novel fused multicyclic iminosugars were synthesized from D-ribose tosylate,aniline and vinyl ethyl ether by one-pot three-component stereoselective[4+2]reaction at different *** iminium-ion is the key intermediate for the *** a result,several complex fused iminosugars 3a were obtained by aza-Diels-Alder mechanism at 60℃,while a series of aza-C-glycosides 5a were prepared by Mannich reaction at room temperature accompanied by another tetrahydroquinoline-fused iminosugars 4a(tricyclic derivatives)through aza-Diels-Alder *** strategy will help to construct structurally diverse and bioactive iminosugar analogues.

关键词： Fused multicyclic iminosugar Aza-Diels-Alder cycloaddition Mannich reaction Tetrahydroquinoline Multicomponent reactions Enantioselectivity Cyclization

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SiC Double Trench moSFET with Split Gate and Integrated Schottky Barrier Diode for Ultra-low Power Loss and Improved Short-Circuit Capability

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Chinese Journal of Electronics 2024年第5期33卷 1127-1136页

作者： Jinping ZHANG Qinglin wu Zixun CHEN hua ZOU Bo ZHANG State Key Laboratory of Electronic Thin Films and Integrated Devices University of Electronic Science and Technology of China

A silicon carbide（SiC） double trench metal-oxide-semiconductor field effect transistor（DTmoS） with split gate（SG） and integrated Schottky barrier diode（SBD） is proposed for the first *** proposed device features two enhanced deep trenches in the surface,in which a source-connected SG with a thicker dielectric layer is located at the bottom of the deep gate trench and an integrated SBD is located at the sidewall of the deep source trench（DST）.Combined with shielding effect provided by the P+shield layer under the DST and integrated SBD,the proposed structure not only reduces the reverse transfer capacitance(Crss) and gate-drain charge(Qgd) but also restrains the saturation drain current(Id,sat) and improves the diode performance of the *** analysis results show that compared with the Con-DTmoS and Con-DTmoS with external SBD diode,the turn-on loss(Eon) and turn-off loss(Eoff) for the proposed device are reduced by 56.4%/70.4% and 56.6%/69.9%,***,the Id,satat the Vgsof 18 V for the proposed device is reduced by 74.4% and the short-circuit withstand time(tSC) is improved by about 7.5 *** a result,an ultra-low power loss and improved short-circuit capability is obtained for the proposed device.

关键词： Performance evaluation Schottky diodes Time-frequency analysis Silicon carbide Simulation Schottky barriers Low power electronics Metal-oxide-semiconductor field effect transistor (moSFET) Specific on resistance Reverse transfer capacitance High frequency figure of merit Forward conduction voltage drop Turn-on loss Turn-off loss Saturated drain current Short-circuit withstand time

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Regulating the key performance parameters for Hg-based IR NLO chalcogenides via bandgap engineering strategy

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Chinese Chemical Letters 2024年第10期35卷 516-520页

作者： A-Yang Wang Sheng-hua Zhou Mao-Yin Ran Xin-Tao wu hua Lin Qi-Long Zhu State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences (CAS) Fujian Science & Technology Innovation Laboratory for Optoelectronic Information of China College of Chemistry Fuzhou University University of Chinese Academy of Sciences

Recently, non-centrosymmetric（NCS） Hg-based chalcogenides have garnered significant interest due to their strong second-harmonic-generation intensities(deff), making them attractive candidates for infrared nonlinear optical（IR-NLO） application. However, achieving both wide band gaps（Eg） and large phasematched deffsimultaneously in these materials remains a challenge due to their inherent constraints on each other. In this research, we have successfully obtained two quaternary NCS Hg-based chalcogenides, Rb2HgGe3S8and Cs2HgGe3S8, by implementing a bandgap engineering strategy that involves alkali metal introduction and Hg/Ge ratio regulation. Both compounds consist of 2D [Hg Ge3S8]2–anionic layers made of 1D [HgGeS6]6–chains and dimeric [Ge2S6]4–polyhedra arranged alternately, and the charge-balanced Rb+/Cs+cations located between these layers. Remarkably, Rb2HgGe3S8and Cs2HgGe3S8exhibit overall properties required for promising IR-NLO materials, including sufficient PM deff（0.55–0.70 × AgGaS2@2050 nm）, large Eg（3.27–3.41 e V）, giant laser-induced damage thresholds（17.4–19.7 ×AgGaS2@1064 nm）, broad optical transmission intervals（0.32–17.5 μm）, and suitable theoretical birefringence（0.069–0.086@2050 nm）. Furthermore, in-depth theoretical analysis reveals that the exceptional IRNLO performance is attributed to the synergy effects of distorted [HgS4] and [GeS4] tetrahedra. Our study provides a useful strategy for enhancing the Egand advancing Hg-based IR-NLO materials, which is expected to extended and implemented in other chalcogenide systems.

关键词： Bandgap engineering strategy Hg-based chalcogenides IR nonlinear material Structure-activity relationship Wide energy gap

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