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Modulating the Oxidation Degree of Linear Polyethyleneimine for Preparation of Highly Efficient and Low-cytotoxicity Degradable Gene Delivery Carriers

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Chinese Journal of Polymer Science 2024年第11期42卷 1699-1709,I0008页

作者： Xiao-Ya Guo Zhi-yu Yang hua-Pan Fang Dan-hua Zhou Xuan Pang hua-yu tian Xue-Si Chen Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefei230026China State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringXiamen UniversityXiamen361005China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) Xiamen361005China

Polyethyleneimine(PEI),as a widely used polymer material in the field of gene delivery,has been extensively studied for modification and shielding to reduce its ***,research aimed at preparing degradable PEI is *** this work,the hydrogen peroxide(H_(2)O_(2))oxidation method was used to introduce degradable amide groups in the PEI and a series of oxidized PEI22k(oxPEI22k)with different degrees of oxidation were synthesized by regulating the dosage of H_(2)O_(2).The relationship between the oxidation degree of oxPEI22k and the gene transfection efficiency of oxPEI22k was studied in detail,confirming that the oxPEI22k with oxidation degrees of 16.7%and 28.6%achieved improved transfection efficiency compared to unmodified *** oxPEI22k also proved reduced cytotoxicity and improved ***,this strategy was extended to the synthesis of low-molecular-weight *** oxPEI1.8k with suitable oxidation degree also achieved improved transfection efficiency and reduced *** brief,this work provided high-efficiency and low-cytotoxicity degradable gene delivery carriers by regulating the oxidation degree of PEI,which was of great significance for promoting clinical applications of PEI.

关键词： Polyethyleneimine Gene delivery Gene transfection Degradable gene carriers

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PLG-g-mPEG Mediated Multifunctional Nanoparticles for Photoacoustic Imaging Guided Combined Chemo/Photothermal Antitumor Therapy

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Chinese Journal of Polymer Science 2023年第4期41卷 538-546,I0007页

作者： Chong Liu Ling Li Zhao-Pei Guo Lin Lin Yan-Hui Li hua-yu tian School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun130022China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China College of Chemistry and Chemical Engineering Xiamen UniversityXiamen361005China School of Materials Science and Engineering Xiamen University of TechnologyXiamen361024China

Under laser irradiation,photothermal therapy(PTT)effectively ablates tumors above 50℃.However,hyperthermia can cause additional damage due to the inevitable heat spread to surrounding healthy ***,nanoparticles named as GI@P NPs were designed for enhanced PTT with heat shock protein 90(HSP90)inhibition at temperatures below 50℃to achieve optimal cancer therapy and avoid surrounding ***@P NPs were done by co-loading Garcinia cambogia acid(GA)and photosensitizer IR783 in polymer PLG-g-mPEG to form a nanomedicine,where IR783 with excellent photoacoustic(PA)signal acted as an excellent photothermal therapeutic agent that converted the laser energy into heat to kill tumor cells,GA was used as antitumor drug for chemotherapy and an inhibitor of HSP90 to overcome the heat resistance of tumors for efficient cryo-photothermal therapy,and PLG-g-mPEG can encapsulate IR783 and GA to increase biocompatibility and accumulate effectively in the *** GI@P NPs were injected into the mice,we could observe that the PA signals gradually increased in the tumor region and showed the strongest PA signals at 12 *** laser irradiation,the tumor temperature of the mice could raise to about 43.5℃,and the tumor was significantly inhibited after long-term monitoring by PA *** a result,gentle PTT produced by GI@P NPs exhibited good antitumor effects at relatively low temperature and minimized nonspecific thermal damage to normal *** GI@P NPs as nanomedicine enriched our understanding of various applications of polymeric carriers,especially in the biomedical field.

关键词： Mild-temperature photothermal therapy Photoacoustic imaging Polymer carrier Combination therapy Co-loading

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Anti-tumor effects of combined doxorubicin and siRNA for pulmonary delivery

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Chinese Chemical Letters 2017年第4期28卷 807-812页

作者： Cai-Na Xu hua-yu tian Yan-Bing Wang Yang Du Jie Chen Lin Lin Zhao-Pei Guo Xue-Si Chen Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Sdences Changchun 130022 China

Direct administration of drugs and genes to the lungs by pulmonary delivery offers a potential effective therapy for lung *** this study,combined doxorubicin（DOX） and Bcl2 siRNA was employed for cancer therapy using polyethylenimine（PEI） as the carrier of Bcl2 *** of the DOX and siRNA possessed high cellular uptake efficiency in B16F10 cells,which was proved by FCM and CLSM ***-time PCR showed that PEI/Bcl2 siRNA exhibited high gene silencing efficiency with 70%Bcl2 mRNA being knocked *** combination of DOX and siRNA could enhance the cell proliferation inhibition and the cell apoptosis against B16F10 cells compared to free DOX or PEI/Bcl2 ***,the biodistribution of DOX and siRNA via pulmonary administration was studied in mice with B16F10 metastatic lung *** results showed that most of the DOX and siRNA were accumulated in lungs and lasted at least for 3 days,which suggested that combined DOX and siRNA by pulmonary administration may have high anti-tumor effects for metastatic lung cancer treatment in vivo.

关键词： Pulmonary delivery Anti-tumor effects Metastatic lung cancer Combined DOX and siRNA therapy Synergistic anti-tumor

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A preliminary report on soil dematiaceous hyphomycetes from the three river gorge regions in eastern Tibet

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菌物学报 2008年第1期27卷 39-47页

作者： GENG yue-hua ZHANG tian-yu WANG Hong-Feng Department of Plant Pathology Shandong Agricultural University Tai ＇an 271018 China

A total of 47 isolates of soil dematiaceous hyphomycetes belonging to 29 species in 15 genera were found from the gorge regions of three rivers (the Jinsha River, the Nu River and the Lantsang River) in eastern Tibet. Most of the genera and species found are new for the regions. Among them Eladia tibetensis is a new species, and Scytalidium acidophilum and S. circinatum are new records to China. Latin description is given for the new species. Brief descriptions and illustrations of the new records are also given based on Chinese isolates. The other 26 species being previously known from China in several genera are also listed. Dried cultures and living cultures studied have been deposited in Herbarium of Shandong Agricultural University: Plant Pathology (HSAUP).

关键词：三江峡谷地带西藏土壤真菌中国新种新记录生物学特征暗色丝孢菌

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Emissions of volatile organic compounds from heated needles and twigs of Pinus pumila

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Journal of Forestry Research 2011年第2期22卷 243-248页

作者： ZHAO Feng-jun SHU Li-fu WANG Qiu-hua WANG Ming-yu tian Xiao-ruiInstitute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry Forest Protection Laboratory of State Forestry Administration,Beijing 100091,P.R.China. Institute of Forest Ecology Environment and Protection Chinese Academy of Forestry Forest Protection Laboratory of State Forestry Administration Beijing P. R. China

A study was conducted to explore the mechanism that emissions of volatile organic compounds（VOC） from heated needles and twigs（200°C,within 15 min） of Pinus pumila affect fire behaviours using the technology of Thermal Desorption-Gas Chromatography-Mass Spectrometry（TD-GC-MS）.The results indicated that the main components of VOC from heated needles and twigs are *** of these terpenoids are *** account for 72.93% for the needles and 92.40% for the twigs of the total VOC,and their emission ratios are 61.200 μg·g-1 and 217.060 μg·g-1 *** twigs can emit more terpenoids than heated needles because twigs had more volatile oils than *** actual fires,these large amounts of terpenoid emissions,especially the monoterpene emissions,have strong effects on fire behaviors that are not only in the initial stage but also in the fast propagation stage of *** flammable gases are capable of causing violent combustion and creating crown *** addition,if these gases accumulate in an uneven geographical area,there will be a possible for eruptive fires and/or fires flashover to occur.

关键词： forest fire Pinus pumila volatile oil volatile organic compounds(VOC) terpenoids

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聚乙烯亚胺介导siRNA和顺铂协同抗肿瘤治疗

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高分子学报 2015年第1期25卷 127-132页

作者：焦自学陈杰田华雨陈学思中国科学院长春应用化学研究所生态环境高分子材料重点实验室长春130022 中国科学院大学北京100049

用聚乙烯亚胺(PEI)为载体,介导siRNA(si Survivin)沉默肿瘤细胞抗凋亡基因survivin,并与抗癌药物(顺铂)进行协同抗肿瘤治疗.凝胶阻滞电泳实验显示,PEI能够对siRNA进行有效复合,在PEI/siRNA质量比为0.4时实现完全阻滞.细胞耐药性实验证... 详细信息

用聚乙烯亚胺(PEI)为载体,介导siRNA(si Survivin)沉默肿瘤细胞抗凋亡基因survivin,并与抗癌药物(顺铂)进行协同抗肿瘤治疗.凝胶阻滞电泳实验显示,PEI能够对siRNA进行有效复合,在PEI/siRNA质量比为0.4时实现完全阻滞.细胞耐药性实验证明了耐顺铂细胞(A549DDP细胞)的survivin基因过度表达且耐顺铂能力是顺铂敏感细胞(A549细胞)的8倍.RT-PCR实验验证了PEI担载si Survivin后对survivin基因实现了有效沉默,与顺铂药物共同作用后不影响基因沉默效果.细胞凋亡实验验证了基因与药物协同作用后细胞的凋亡率达到60.9%,而单独药物或PEI/si Survivin复合物分别作用后的细胞凋亡率仅分别为30.2%和19.8%.细胞增殖实验进一步验证了PEI介导si Survivin与顺铂联合治疗能够实现有效地协同抗肿瘤效果.

关键词：聚乙烯亚胺 Survivin基因顺铂协同作用抗肿瘤

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Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： Jing Li Zixuan He Weitai Chai Meng tian huali yu Xiaoxiao He Xiaojuan Zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan

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部分发酵类肠杆菌科细菌耐药性及耐碳青霉烯类基因检测分析

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中国病原生物学杂志 2017年第9期12卷 818-824页

作者：王祖华田雨桐景瑞婷余春芳金志雄湖北医药学院基础医学院湖北十堰442000

目的检测部分发酵类肠杆菌科细菌对临床推荐用抗生素的敏感性并筛查耐碳青霉烯类基因。方法采集鄂西北地区2013年1-12月间住院病人的血、痰、中段尿、前列腺液、分泌物或引流液等,常规琼脂培养法分离发酵类肠杆菌肺炎克雷伯菌(Klebsiell... 详细信息

目的检测部分发酵类肠杆菌科细菌对临床推荐用抗生素的敏感性并筛查耐碳青霉烯类基因。方法采集鄂西北地区2013年1-12月间住院病人的血、痰、中段尿、前列腺液、分泌物或引流液等,常规琼脂培养法分离发酵类肠杆菌肺炎克雷伯菌(Klebsiella pneumoniae)、产酸克雷伯菌(Klebsiella oxytoca)、大肠埃希菌(Escherichia coli)、阴沟肠杆菌(Enterbacter cloacae)及沙雷氏菌(Serratia);利用Vitek-2分析系统鉴定菌株并检测其耐药性;PCR法扩增抗碳青霉烯类(亚胺培南和/或美罗培南)菌株耐药基因。结果共获得2 291株肺炎克雷伯菌、大肠埃希菌、阴沟肠杆菌和沙雷氏菌等分离株,产ESBLs率分别为47.4%、62.6%、9.1%、22.6%;临床推荐用抗生素耐药率:头孢唑啉(Ⅰ)分别为45.4%、73.7%、93.3%、100%,头孢西丁(Ⅱ)分别为17.3%、15.2%、84.9%、33.3%,头孢曲松(Ⅲ)分别为48.4%、65.0%、43.8%、44.4%;阿米卡星分别为17.4%、10.4%、28.1%、24.4%,氨曲南分别为35.4%、38.9%、49.1%、38.9%,亚胺培南(分别为1.8%、1.8%、17.6%、2.4%,美罗培南分别为1.1%、0.8%、6.7%、20%。其中分离出46株耐亚胺培南和/或美罗培南菌株,肺炎克雷伯菌P2964和肺炎克雷伯菌B195携带blaKPC-2基因,肺炎克雷伯菌P6558和产酸克雷伯菌B1645-1携带blaNDM-1基因。结论鄂西北地区部分发酵类肠杆菌耐药率呈上升趋势并出现耐碳青霉稀类菌株,菌株间可能存在耐药基因的传递并有造成感染扩散的风险,尤其在住院病人之间,应予以高度重视。

关键词：肠杆菌科细菌碳青霉烯类 NDM-1 耐药性

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EST-SSR Sequences Revealed the Relationship of D Genome in Diploid and Tetraploid Species in Gossypium

EST-SSR Sequences Revealed the Relationship of D Genome in D...

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江苏省遗传学会第七届二次代表大会暨学术研讨会

作者： hua-yu Zhu, tian-Zhen Zhang, Lu-Ming Yang, Wang-Zhen Guo * (National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China)

＜正＞Tetraploid cotton species were synthesized by both A- and D-compound genomes. Prior to this study, the D-genome donor in tetraploid cotton species remained elusive. In a previous study,

关键词： Gossypium cotton EST-SSR D-genome diploid tetraploid genetic relationship

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超声波辅助钎焊铜与石墨的组织及性能

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兰州理工大学学报 2017年第5期43卷 30-34页

作者：俞伟元尚江旭颜泽华张天宇高盟召兰州理工大学省部共建有色金属先进加工与再利用国家重点实验室甘肃兰州730050

采用四元合金Ag30Cu25Zn4Ti作为活性钎料,在大气中利用超声波辅助钎焊技术对铜和石墨进行了钎焊.通过改变超声波作用时间和方式,研究了不同超声波作用时间下钎料氧化膜破碎的效果,及其与TiC反应层形貌之间的关系.采用扫描电子显微镜对... 详细信息

采用四元合金Ag30Cu25Zn4Ti作为活性钎料,在大气中利用超声波辅助钎焊技术对铜和石墨进行了钎焊.通过改变超声波作用时间和方式,研究了不同超声波作用时间下钎料氧化膜破碎的效果,及其与TiC反应层形貌之间的关系.采用扫描电子显微镜对钎焊接头的微观组织进行了观察,最后对钎焊的接头进行了力学性能分析.结果表明:随着超声波作用时间的延长,液态钎料中的氧化膜逐渐减少,最终完全消除;与此同时,TiC反应层的厚度也在逐渐增加,最终趋于稳定.钎焊接头的组织由铜侧到石墨侧依次主要为Cu/Cu-Zn金属间化合物,Ag基固溶体和Cu基固溶体/TiC/石墨,钎焊接头的剪切强度最大值为18 MPa,界面的结合主要是超声波作用下活性元素Ti在石墨侧的偏聚形成TiC反应层而形成的.

关键词：超声波辅助铜石墨氧化膜反应层

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